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Facilities

ICGEB Working Group on Zika Virus

In February 2016, the World Health Organization (WHO) declared the Zika virus outbreak in South and Central American a Public Health Emergency of International Concern (PHEIC). A coordinated international response is foreseen in order to concentrate resources on disease surveillance, mosquito control and research in an attempt to control the spread of infection in the… Read more »

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ARMOUR Arice miRNA

mRNA Interaction Resource Link at: https://armour.icgeb.trieste.it/login Plant RNAi Biology Biology Laboratory Research Interests and Description Neeti Sanan-Mishra International Centre for Genetic Engineering and Biotechnology Aruna Asaf Ali Marg 110 067 New Delhi, India E-mail: Tel: +91-11-26741358, ext. 280Fax: +91-11-26742316

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avu-core-facility

AVU Core Facility The AVU (AAV Vector Unit) is a Core Facility for the production and characterization of viral vectors based on the Adeno-Associated Virus (AAV), a small and defective human parvovirus. The characteristics of recombinant AAV vectors, including the non-pathogenicity, the low immunogenicity and the capacity to transduce quiescent cells, render them suitable for… Read more »

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avu-facilities-d1

AVU Core Facility Head: Lorena Zentilin Technicians: Marina Dapas, Michela Zotti AAV has a single-stranded DNA genome containing two genes. The Rep genes codes for the replicative proteins; the Cap genes gives rise, through alternative splicing, to 3 structural capsid proteins. One is the Rep gene coding for the replicative proteins, and the other is… Read more »

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Facilities

Pathogenic Virus Manipulation (BL3) The Biosafety Level 3 (BL3) Laboratory at ICGEB Trieste was designed in accordance with the guidelines of the Italian Ministry of Health. This facility has special engineering and containment features that allow investigators to work safely with known or possible human pathogens classified at level 3 such as the human immunodeficiency… Read more »

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Safety Procedures

Biosafety Level 3 Laboratory (BL3) Personnel The BL3 facility should be used routinely within working hours (8.00 – 17.00). No unauthorised person are permitted in the BL3 area. Certain experienced visitors may be permitted to enter the BL3 if accompanied by an authorised senior staff individual, upon prior clearance. Personnel with prior HIV or BL3… Read more »

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Facilities

Flow Cytometry (FACS) The ICGEB Flow Cytometry Facility is supplied with three different instruments, for a wide range of possible flow cytometry applications. Simple analysis of few parameters is performed on the BD Accuri C6 Plus, a cytometer with two excitation lasers (blue, 488 nm, and red, 633 nm) and four fluorescence reading channels at… Read more »

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Facilities

Fluorescence Microscopy (FMCF) The Fluorescence Microscopy Core Facility (FMCF) at the ICGEB Trieste Component offers a collection of state-of-the-art fluorescence microscopy equipment and accessory services to support in-house scientists and visitors in the use of fluorescence microscopy methods for their research. Currently, the facility offers: (i) two upright fluorescence microscopy settings, one equipped with Leica… Read more »

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Gel/Immunoblot Imaging

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High Throughput Screening

Overview The High-Throughput Screening (HTS) Facility was established at the ICGEB Trieste in 2008 as a result of a collaborative project involving four research centres in Northeast Italy (ICGEB Trieste, LNCIB, SISSA, CIME), financed by the Friuli-Venezia-Giulia Regional Government. The main goal of the High-Throughput Screening (HTS) Facility is to provide the research community with a platform of… Read more »

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High Throughput Screening libraries

Libraries siRNA libraries Aiming at providing the best available RNA interference technology with maximum flexibility, the HTS Facility at the ICGEB offers to researchers both a human and a mouse whole-genome siRNA library. These libraries are composed of pools of 4 different siRNAs per gene (siGENOME SMARTPool technology, Dharmacon). The availability of both a human… Read more »

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High Throughput Screening data analysis

Data Analysis For storage of screening data, in particular from high-content screening experiments, a dedicated server with a redundant storage capacity of 64Tb has been implemented in the HTS Facility; two computers are dedicated to offline data analysis. The following software packages are available at the HTS Facility for data analysis: MetaXpress, including 10 application… Read more »

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