Pathogenic Virus Manipulation (BL3) The Biosafety Level 3 (BL3) Laboratory at ICGEB Trieste was designed in accordance with the guidelines of the Italian Ministry of Health. This facility has special engineering and containment features that allow investigators to work safely with known or possible human pathogens classified at level 3 such as the human immunodeficiency […]

Biosafety Level 3 Laboratory (BL3) Personnel The BL3 facility should be used routinely within working hours (8.00 – 17.00). No unauthorised person are permitted in the BL3 area. Certain experienced visitors may be permitted to enter the BL3 if accompanied by an authorised senior staff individual, upon prior clearance. Personnel with prior HIV or BL3 […]

Who we are The ICGEB Flow Cytometry Facility is supplied with three different instruments for a wide range of possible flow cytometry applications. Simple analysis of a few parameters is performed on the BD Accuri C6 Plus, a cytometer with two excitation lasers (blue, 488 nm, and red, 633 nm) and four fluorescence reading channels […]

Fluorescence Microscopy (FMCF) The Fluorescence Microscopy Core Facility (FMCF) at the ICGEB Trieste Component offers a collection of state-of-the-art fluorescence microscopy equipment and accessory services to support in-house scientists and visitors in the use of fluorescence microscopy methods for their research. Currently, the facility offers: (i) two upright fluorescence microscopy settings, one equipped with a […]

Overview The High-Throughput Screening (HTS) Facility was established at the ICGEB Trieste in 2008 as a result of a collaborative project involving four research centres in Northeast Italy (ICGEB Trieste, LNCIB, SISSA, CIME), financed by the Autonomous Region Friuli-Venezia-Giulia. The goal of the High-Throughput Screening (HTS) Facility is to provide the research community with a platform of up-to-date […]

Peptide Synthesis The Peptide Laboratory employs techniques of solid and solution phase chemistry with automated or manual peptide synthesis based on FMOC chemistry on a 0.05 to 0.40 miliomolar scale. The peptides are purified by preparative RP-HPLC up to >98% and liquid chromatography coupled with mass spectrometry used as quality control. The lab has experience […]

Proteomics Facility The Proteomics Facility uses state of the art techniques in mass spectrometry for protein identification, protein quantification, and the mapping of post translational modifications.  The facility is equipped with modern electrophoresis and HPLC equipment for sample preparation and separation.  The facility is currently equipped with two mass spectrometers:  a Thermo Finnigan LCQdeca ion […]