Head of Genomics Research Centre- Human Technopole, Milano, (ITALY) & Team Leader of Laboratory for Transcriptome Technology, RIKEN Center for Integrative Medical Sciences, Yokohama, JAPAN
Genome regulation by long non-coding RNAs
Host: G. Del Sal
The genome produces a large variety of long non-coding RNAs (lncRNAs). The relatively few well studied lncRNAs are engaged in a variety of activities, including interactions with chromatin, other RNAs, proteins and may have regulatory or structural role. Together with lncRNAs, the genome is also regulated by many proteins, including transcription factors, epigenome modifiers and other DNA interacting proteins. Altogether, all these molecules form complexes that interact and regulate chromatin, promoters and enhancers in the human body with high cell specificity in health and diseases in different genetic background.
To address these challenges, the Carninci Group strive to develop and broadly use technologies to comprehensively study the non-protein-coding part of the genome, its function and interactome. The following are some of the focuses of the laboratory: i) we study the role, structure, modifications and interactome of SINE elements embedded in antisense lncRNAs, which are involved in the regulation of translation of the gene they target; ii) we develop and standardize transcription profiling technologies like the cap-analysis gene expression (CAGE), to develop a universal and finely quantitative transcriptome technology, which will ultimately be used to profile populations of single cells in tissues; finally we further develop approaches to detect interactomes of molecules, like for the RADICL-seq technology, which globally detects interactions of RNA with chromatin and identifies RNAs that are likely to regulate gene activity. –
Altogether, understanding the function of the non-coding regulatory genome is essential to develop genome medicine, to fully realize the potential of genomics for human health.
International Seminar Programme
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