Mirco Vanetti

Thursday, 25 October | 3:00 pm

European Applications manger-Functional Genomics at IDT

Mitigating the risk of off-target effects when using CRISPR genome editing

(Host: A. Marcello)

During this presentation you will learn about:

  • Optimized strategies for CRISPR-Cas9 mediated genome editing, with emphasis on methods commonly used for delivery of guide RNAs and Cas9 as a ribonucleoprotein complex
  • The risk of off-target effects (OTEs): how to find and measure the frequency of off-target effects (OTEs) including discussion of bioinformatic screening for OTEs and methods for unbiased CRISPR OTE detection
  • Recent developments in CRISPR research, including a noval mutant Cas9 protein that has been evolved to reduce off-target gene editing while maintaining on- target potency