Francesca GIANNESE and Dejan LAZAREVIC

Coordinator of the Innovation Lab research area, Center for Omics Sciences at IRCCS Ospedale San Raffaele, Milan, ITALY

and Coordinator of the Genomics research area, Center for Omics Sciences at IRCCS Ospedale San Raffaele, Milan, ITALY

Friday, 25 January 2022 | 12:00 noon – ICGEB Trieste, ITALY

Single cell analysis: theory and practice

Host: F. Benvenuti

ISingle-cell sequencing technologies allow the analysis of transcriptomic, genomic, epigenetic information of individual cells to characterise cellular heterogeneity and evolutionary relationships. Indeed, this analysis overcome limitations of standard bulk sequencing methods which can provide only an average omic profile of many cells. We will discuss the technological improvements in this field describing the most common approaches available to perform single cell analysis. We will also introduce single-cell sequencing applications, such as describing lineage specification and cellular heterogeneity in disease pathogenesis. Our presentation will be followed by a practical demo of the 10x Genomics microfluidic platform Chromium.

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