INDUSTRIAL BIOTECHNOLOGY / Biofuels and Industrial Biotechnology
Second generation biofuels, fungal and bacterial genome engineering, engineered cellulases, C5/C6 fermentation, n- and iso-butanol production, microbial alkane and cetearyl alcohol production.
Description of Research
Discovery and design of novel enzymes and enzyme systems for biofuels
Recent interest towards shifting to non-food-based feedstock for biofuel production, in addition to biomass burning issue, has resulted in exploiting agricultural residues as feedstock. However, recalcitrant nature of this biomass makes it extremely difficult to hydrolyze into fermentable sugar. The filamentous fungi are found to be efficient in carbon cycling in nature, and thus are treated as potential sources of enzymes for converting recalcitrant lignocellulosic biomass into precursors for industrial purpose. We use intense mathematical model based screen to identify fungal isolates whose secretome can degrade biomass more efficiently than commercial cellulase formulation. Several enzymes have been characterized and among them cellobiohydrolases of the glycoside hydrolase family 7 (CBH1), was found to be the most important cellulolytic enzymes for crystalline cellulose breakdown. CBH1 of a new fungal isolate exhibited 6-fold higher catalytic efficiency as well as a 26-fold higher enzyme-inhibitor complex equilibrium dissociation constant (Ki) than the one from Trichoderma reesei. We have performed genome engineering of the new fungal isolate to disrupt its catabolite repressor and overexpress the cellulase gene activator to enhance the enzyme production by several folds. Many leads have been obtained to understand the role of several unannotated transcription factors involved in the cellulase production via transcriptomic and proteomic studies, which are being valorized to construct superior biocatalyst.
Engineering bacteria to produce biofuels and biochemicals
Lignocellulosic biomass consists of ~30% of pentose sugars, and therefore its effective fermentation will certainly have a positive impact on the economy of biofuel production. The traditional yeast, Saccharomyces cerevisiae, is unable to ferment pentose sugars into ethanol. E. coli is a robust host for various genetic manipulations and can readily consume both hexose and pentose sugars. However, availability of limited reducing equivalence and generation of competing co-products undermine ethanol yield and productivity in this microbe. In our lab, we have constructed an E. coli strain to produce high yield of ethanol from hexose and pentose sugars by modulating endogenous pathway without the need of foreign genes. We further worked towards formation of more energy dense fuel molecules, such as butanol, hexanol, pentadecane and hexadecene. Butanol was made in E. coli by integrating Clostridial pathway in its genome via CRISPR/Cas9 technique. On the other hand, long chain alkanes and alcohols are being made via metabolic model-assisted engineering of E. coli, with the highest titers reported so far.
Ogunyewo OA, Upadhyay P, Rajacharya GH, Okereke OE, Faas L, Gómez LD, McQueen-Mason SJ, Yazdani SS. Accessory enzymes of hypercellulolytic Penicillium funiculosum facilitate complete saccharification of sugarcane bagasse. Biotechnology for biofuels. 2021 Aug 26;14(1):171.
Sharma A, Shakeel T, Gupta M, Rajacharya GH, Yazdani SS. Biophysical and structural studies reveal marginal stability of a crucial hydrocarbon biosynthetic enzyme acyl ACP reductase. Scientific Reports. 2021 Jun 8;11(1):12045.
Randhawa, A., Ogunyewo, O.A., Eqbal, D., Gupta, Randhawa A, Pasari N, Sinha T, Gupta M, Nair AM, Ogunyewo OA, Verma S, Verma PK, Yazdani SS. Blocking drug efflux mechanisms facilitate genome engineering process in hypercellulolytic fungus, Penicillium funiculosum NCIM1228. Biotechnology for biofuels. 2021 Dec;14(1):31.
Ogunyewo OA, Randhawa A, Gupta M, Kaladhar VC, Verma PK, Yazdani SS. Synergistic Action of a Lytic Polysaccharide Monooxygenase and a Cellobiohydrolase from Penicillium funiculosum in Cellulose Saccharification Under High Substrate Loading. Applied and Environmental Microbiology 2020 Sep 25:AEM.01769-20.
Pasari, N., Adlakha, N., Gupta, M., Bashir, Z., Jawed K, Abdelaal AS, Koffas MAG, Yazdani SS. Improved Butanol Production Using FASII Pathway in E. coli. ACS Synthetic Biology 2020; 9(9):2390-2398.
Abdelaal AS, Jawed K, Yazdani SS. CRISPR/Cas9-mediated engineering of Escherichia coli for n-butanol production from xylose in defined medium. Journal of Industrial Microbiology and Biotechnology 2019 July 1; 46(7):965-975
Yazdani SS, Olusola A. Ogunyewo , Randhawa A, Gupta M. 2021. Enzyme Overexpression for Optimized Lignocellulosic Degradation.PCT filed Granted Patent No.: PCT/IB2021/052791
Yazdani, S.S., Funso, E. 2020. A method for obtaining a composition for biomass hydrolysis. US Patent Application No.: US10526593B2
Yazdani, S.S., Fatma, Z. 2019. CRISPR/CAS9 mediated engineering of Escherichia coli strains for n-butanol production from xylose and glucose in defined medium”.
Indian Patent Application No. 4201911014864
Yazdani, S.S., Munjal, N., Mattam, A.J. 2017. Modified bacteria for the production of bioalcohols. US, Chinese and Indian Patent GrantedNo: US9631206B2; CN104838005B