Shashi Kumar

Group leader, Metabolic Engineering

International Centre for Genetic Engineering and Biotechnology
Aruna Asaf Ali Marg
110 067 New Delhi, India

E-mail: skrhode@icgeb.res.in
Tel: +91-11-26741358/26742357 ext 475

Education

University of Delhi South Campus, Department of Genetics, New Delhi, India, PhD, 1998
India Institute of Technology (IIT), Biosciences and Biotechnology, MSc, 1991
Meerut University, Zoology, Botany, Chemistry, BSc, 1988

Career History

2011- Group Leader, Metabolic Engineering, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
2009-2011, Scientist, Malaria Group, ICGEB New Delhi, India
2006-2009, Senior Scientist, Yulex Inc., USA
2005-2006, Research Specialist, UC Berkeley, CA, USA
2001-2005, Research Associate, UCF, FL, USA
1998-2001, Research Associate, University of Virginia, VA, USA

Scientific Activity

Metabolic engineering
Synthetic biology
Genome editing
Chloroplast genome engineering
Nuclear genome transformation
Sustainable algae biofuel technology
Phycoremediation of wastewater
Drugs biosynthesis in photosynthetic organisms

Selected publications

Bhatnagar VP, Bandyopadhyay P, Rajacharya GH, Sarkar S, Poluri KM, Kumar S (2019) Amelioration of biomass and lipid in marine alga by an endophytic fungus Piriformospora indica. Biotechnology for Biofuels,  2019 Jul 8;12:176. doi: 10.1186/s13068-019-1516-6. eCollection 2019. PMID: 31316583, PMCID: PMC6613240, DOI: 10.1186/s13068-019-1516-6 [IF:5.4]

Augustine A, Tanwar A, Tremblay R, Kumar S. (2019) Flocculation processes optimization for reuse of culture medium without pH neutralization. Algal Research, 39: 101437. https://doi.org/10.1016/j.algal.2019.101437 [IF:5.0]

Nawkarkar P, Singh AK, Abdin MZ, Kumar S (2019) Life cycle assessment of Chlorella species producing biodiesel and remediating wastewater. Journal of Biosciences, 44: 89. https://doi.org/10.1007/s12038-019-9896-0 [IF:1.8]

Nawkarkar P, Chugh S, Tanwar A,  Sharma S, Kaushal C, Abdin MZ, Jain M, Kumar S (2019). Chloroplast genome analysis and genetic transformation of Parachlorella kessleri-I a potential marine alga for biofuel production. BMC Genomics. https://doi.org/10.21203/rs.2.12052/v1 [IF:3.5]

Adeniran AA, Sonibare MA, Rajacharya GH, Kumar S (2018) Assessment of genetic fidelity of Dioscorea bulbifera L. and Dioscorea hirtiflora Benth and medicinal bioactivity produced from the induced tuberous roots. Plant Cell Tiss Organ Cult. 132: 343-357.DOI: 10.1007/s11240-017-1334-0 [IF: 2.0]

Tanwar A, Sharma S, Kumar S (2018). Targeted Genome Editing in Algae using CRISPR/Cas9. Ind J Plant Physiol. 23(4): 653-669. DOI: 10.1007/s40502-018-0423-3

Kaushik P, Kumar S (2018) Transcriptome Analysis of Bael (Aegle marmelos (L.) Corr.) a Member of Family Rutaceae. Forests, 9(8): 450; https://doi.org/10.3390/f9080450

Kaushik P, Kumar S (2018) Data of de novo assembly of the leaf transcriptome in Aegle marmelos. Data in Brief 19: 700–703. https://doi.org/10.1016/j.dib.2018.05.095

Kaushik P, Kumar S, Dhaliwal MS. 2018. Usefulness of Three under Exploited Vegetable Legumes – A Review. Int. J. Curr. Microbiol. App. Sci. 7(03): 861-870. [IF: 4.1; SSN:2319- 7692(Print), ISSN:2319-7706(Online)], https://doi.org/10.20546/ijcmas.2018.703.101

Kunrunmi O, Adesalu T, Kumar S (2017). Genetic identification of new microalgal species from Epe Lagoon of West Africa accumulating high lipids. Algal Research, 22: 68-78. [IF: 5.0; ISSN: 2211-9264]

Singh AK, Sharma N, Farooqi H, Abdin MZ, Mock T, Kumar S (2017) Phycoremediation of municipal wastewater by microalgae to produce biofuel. International J Phytoremediation. 19(9): 805-812. [IF: 2.0; Print ISSN: 1522-6514 Online ISSN: 1549-7879], PMID: 28156133.

Malhotra K, Subramaniyan M, Rawat K, Kalamuddin M, Qureshi MF, Malhotra P, Mohmmed A, Cornish K, Daniell H, Kumar S (2016). Compartmentalized metabolic engineering for artemisinin biosynthesis and effective malaria treatment by oral delivery of plant cells. Molecular Plant, 9(11): 1464-1477.  [IF: 10.8; ISSN 1674-2052]

Singh, D., Kumar, S., Daniell, H. (2016) Expression of Beta-glucosidase increases trichome density and artemisinin content in transgenic Artemisia annua plants. Plant Biotechnology Journal,16: 1034-1045. [IF: 7.4; ISSN 1467-7652]

Ahmad, I., Sharma, A., Daniell, H., Kumar, S. (2015) Altered Lipid Composition and Enhanced Lipid Production in Microalga by Introduction of Brassica Diacylglycerol Acyltransferase 2.Plant Biotechnology Journal, 13: 540-550. [IF: 7.4; ISSN 1467-7652]

Shao, M., Kumar, S., Thomson, J.G. (2014) Precise excision of plastid DNA by the large serine recombinase Bxb1. Plant Biotechnology Journal 12, 322-329. [IF: 7.4; ISSN 1467-7652]

Turan, S., Cornish, K., Kumar, S. (2014) Highly efficient callus-mediated genetic transformation of Parthenium argentatum Gray, an alternate source of latex and rubber. Industrial Crops and Products 62, 212-218. [IF: 4.03; ISSN: 0926-6690]

Truan, S., Kumar, S. Cornish, K., (2014) Photosynthetic response of in vitro guayule plants in low and high lights and the role of non-photochemical quenching in plant acclimation. Industrial Crops and Products 54, 266–271. [IF: 4.03; ISSN 0926-6690]

Heikrujam, M., Kumar, D., Kumar, S., Gupta, S.C., Agrawal, V. (2014) High efficiency cyclic production of secondary somatic embryos and ISSR based assessment of genetic fidelity among the emblings in Calliandra tweedii (Benth.). Scientia Hort, 177, 63-70. [IF: 2.01; ISSN 0304-4238]

Saxena, B., Subramaniyan, M., Malhotra, K., Bhavesh, N.S., Potlakayala, S.D., Kumar, S. 2014. Metabolic engineering of chloroplasts for artemisinic acid biosynthesis and impact on plant growth. J Biosci 39, 1-9. [IF: 1.17; ISSN: 0250-5991 (Print) 0973-7138 (Online)]

Ahmad, I., Fatma, Z., Yazdani, S., Kumar, S. (2013) DNA barcode and lipid analysis of new marine algae potential for biofuel. Algal Research. 2, 10-15. [IF: 5.0; ISSN 2211-9264]

Kumar, S., Baidoo, E., Hahn, F.M., Kahlon, T., Wood, D., McMahan, M.C., Cornish, K., Keasling, J., Daniell, H., Whalen, M.C. (2012) Remodeling the isoprenoid pathway in tobacco by expressing the cytoplasmic mevalonate pathway in chloroplasts. Metabolic Engineering 14: 19-28. [IF: 8.2; ISSN‎ ‎1096-7176 (print); 1096-7184 (web)]

Singh, D., Singh, P.K., Chaudhary, S., Mehla, K., Kumar, S. (2012) Exome sequencing and advances in crop improvement. Advances in Genetics 79, 87-121. [IF: 5; 0065-2660]

Turan, S., Cornish K., Kumar, S. (2012) Salinity Tolerance in Plants: Breeding and Genetic Engineering. Australian Journal of Crop Science  6: 1337-1348. [IF: 1.02; ISSN1835-2693]

Kumar, S., Kahlon, T and Chaudhary, S. (2011) DNA barcodes for unmasking the adulteration in olive oil. Food Chemistry 127: 1335–1341.  [IF: 5; ISSN 0308-8146]

Kumar, S., McMahan, M.C., Cornish, K. and Whalen, M.C. (2009) Comparative analysis of the complete sequence of the plastid genome of Parthenium argentatum and identification of DNA barcodes to differentiate Parthenium species and lines. BMC Plant Biology 2009, 9:131 doi:10.1186/1471-2229-9-131 [IF: 5.3; 1471-2229]

Daniell, H., Kumar, S. and Dufourmantel, N. (2005) Breakthrough in chloroplast genetic engineering of agronomically important crops. Trends in Biotechnology 23: 238-245.  [IF: 14.0; ISSN 0167-7799]

Daniell, H., Chebolu, S., Kumar, S., Singleton, M., Falconer, R. (2005). Chloroplast-derived vaccine antigens and other therapeutic proteins. Vaccine 23:1779-1783. [IF: 3.2; ISSN 0264-410X]

Kumar, S., Dhingra, A. and Daniell, H. (2004) Plastid expressed betaine aldehyde dehydrogenase gene in carrot cultured cells, roots and leaves confers enhanced salt tolerance. Plant Physiology 136:2843-2854 [IF: 6.0; ISSN 1532-2548]

Kumar, S., Dhingra, A. and Daniell, H. (2004) Stable transformation of the cotton plastid genome and maternal inheritance of transgenes. Plant Molecular Biology 56: 203-216. [IF: 3.0; ISSN0167-4412] 

Kumar, S. and Timko, M.P. (2004) Enhanced Tissue-Specific Expression of the Herbicide Resistance bar Gene in Transgenic Cotton (Gossypium hirsutum L cv. Coker 310FR) Using the Arabidopsis rbcS ats1A Promoter. Plant Biotechnology 21: 251-260. [IF: 1.0; ISSN 1342-4580]

Kumar, S. and Daniell, H. (2004) Engineering the chloroplast genome for hyper-expression of human therapeutic proteins and vaccine antigens in Recombinant Protein Protocols. Methods in Molecular Biology 267: 365-383. [IF: 1.4; ISSN 1064-3745]

Kumar, S., Agrawal, V. and Gupta, S.C. (2002) Somatic embryogenesis in the woody legume Calliandra tweedii.Plant Cell, Tissue and Organ Culture. 71:77-80.  [IF: 2.02; ISSN: 0167-6857 (Print) 1573-5044 (Online)]

Kumar, S.  and  Pental, D. (1998) Regeneration of  Indian  Cotton Variety  MCU-5 through  Somatic Embryogenesis. Current Sci 74: 538-540. [IF: 0.8; ISSN 0011-3891]

Kumar, S., Sharma, P. and Pental, D. (1998) A  Genetic Approach to In  Vitro  Regeneration of  Non-Regenerating  Cotton (Gossypium hirsutum L.) Cultivars.  Plant Cell Rep 18: 59-63. [IF: 2.9; ISSN 0721-7714]

Patents

Kumar, S., Singh, A.K. Method of Utilization of Carbon Concentration Mechanism in Micro-Algal Species to Increase Production of Lipids and Obtain Bio-Fuel. Indian Patent Application No: 2856/DEL/2015

Kang, B., McMahan, C.M., Whalen, M.C., Dong, N., Kumar, S. (2016). Engineering rubber production in plants. US 20140325699 (granted). WO/2014/152747 https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2014152747