Pavan Jutur

Group Leader, Omics of Algae
International Centre for Genetic Engineering and Biotechnology
Aruna Asaf Ali Marg
110 067 New Delhi, India
E-mail: [email protected] 
Tel: +91-11-26741358 ext 451

Education

Pondicherry University, Pondicherry, India, PhD, 2005
Pondicherry University, Pondicherry, India, MPhil, 2001
Kuvempu University, Davanagere, Karnataka, India, MSc, 1999

Career History

Since 2012, Team Leader, DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
2009-2012, Postdoctoral Research Associate, University of Nebraska, Lincoln, USA
2008-2009,
Lecturer, BITS-Pilani (Goa Campus), Goa, India
2006-2008, ISSAR Pharma Pvt Ltd/USP India Pvt Ltd, Hyderabad, India
2005-2006, Research Associate, University of Hyderabad, Hyderabad, India

Scientific Activity

Our objective is to understand basic biology and metabolic/regulatory pathways in unicellular photosynthetic model microalga Chlamydomonas reinhardtii regarded as non-oleaginous. While under optimal growth conditions, it synthesizes fatty acids principally for esterification into membrane lipids, under conditions limiting to growth, particularly nutrient deprivation, it slows down the cell proliferation and alters the lipid biosynthetic pathways towards the formation and accumulation of the carbohydrates (starch) and neutral lipid triacylglycerols (TAGs). TAGs are one of the most energy-rich forms of reduced carbon available from nature. They can be converted to biodiesel by trans-esterification with methanol in the presence of an acid or alkali catalyst. C. reinhardtii is unlikely to be chosen for biofuel production. However, it is a model alga whose complete genome is available and used as a reference to understand metabolic and regulatory networks involved in lipid metabolism and TAG accumulation. For economic viability and sustainability of algal biofuels, it is essential to understand the basic biology of potential microalgae, particularly the effects of environmental stresses on cellular metabolisms and the regulation of biosynthetic pathways of fatty acids and TAGs, as well as carbon fixation and allocation. Neutral lipid biosynthesis and turnover have been studied in higher eukaryotes particularly yeast, mice, and the model plant Arabidopsis thaliana where extensive knowledge exists on lipid metabolism and several key enzymes identified. However, these pathways are not fully documented in microalgae and little is known about the enzymes involved in the formation, accumulation, or degradation of TAGs. More research is needed to understand signal perception and transduction under stress and different molecular mechanisms leading to TAG and starch accumulations in microalgae. In this context, rapidly developing systems-level “Omics” analyses (transcriptomics, proteomics, metabolomics), which are both sensitive and quantitative, and the availability of annotated genomes allow us to investigate changes in response to environmental stress. This may provide useful hypotheses for microalgal strain improvement to optimize their biofuel production.

Currently, our research project focuses on:

  • Identification and characterization of robust microalgae from various collections for the genetic improvements for biofuel production.
  • Genome sequencing and annotation of selected microalgae for facilitating pathway engineering.
  • Investigating components of lipid biosynthetic pathway and its regulation in microalgae.
  • Characterization of quality of lipid in the microalgae and corresponding biosynthetic pathways. 
  • Engineering microalgae for improving the quality of lipid for biodiesel production.
  • Study of genetic mechanism and enzymes responsible for self-flocculation and its exploitation in algae.

Our findings will provide important breakthrough on central metabolism in these microalgae, which are required for biotechnological improvement of next-generation biofuel production.

Selected publications

Shaikh, K.M., Kumar, P., Nesamma, A. A., Abdin, M.Z., and P.P. Jutur. Hybrid genome assembly and functional annotation reveals insights on lipid biosynthesis of oleaginous native isolate Parachlorella kessleri, a potential industrial strain for production of biofuel precursors. Algal Research, 52: 102118, 2020

Singh, R., Paliwal, C., Nesamma, A. A., Narula, A., and P.P. Jutur. Nutrient deprivation mobilizes the production of unique tocopherols as a stress-promoting response in a new indigenous isolate Monoraphidium sp. Frontiers in Marine Science, 7: 575817, 2020

Kareya, M. S., Mariam, I., Shaikh, K. M., Nesamma, A. A., and P.P. Jutur. Photosynthetic carbon partitioning and metabolic regulation in response to very-low and high CO2 in M. gaditana NIES 2587. Frontiers in Plant Science, 11: 981, 2020

Kumari, S., Nesamma, A. A., Lali, A. M., Jutur, P. P., and G. Prakash. The chloroplast genome of a resilient chlorophycean microalga Asterarcys sp. Algal Research, 49: 101952, 2020

Rehmanji, M., Suresh, S., Nesamma, A.A., and P.P. Jutur. Microalgal cell factories, a platform for high-value added biorenewables to improve economics of the biorefinery. In Microbial and Natural Macromolecules, edited by Surajit Das, Hirak Dash, Elsevier Publishers Inc., USA, pp. 689-731, 16th September 2020

Shaikh, K. M., Mariam, I., Nesamma, A.A., Abdin, M.Z., and P.P. Jutur. Application of transgenic technologies in biofuel production through photosynthetic chassis – new paradigms from gene mining to genome editing. In Transgenic Technology Based Value addition in Plant Biotechnology, edited by M. Z. Abdin, Usha Kiran, Elsevier Publishers Inc., USA, pp. 227-245, July 2020

Kareya, M. S., Mariam, I., Nesamma, A. A., and P.P. Jutur. CO2 sequestration by hybrid integrative photosynthesis (CO2SHIP) – A green initiative for multi-product biorefineries. Materials Science for Energy Technologies, 3: 420-428, 2020

Kannikka, B., Pasupuleti, S. C., Monika, J., Mahima, S., Ashish, M., Kareya, M. S., Jutur, P. P., Amit, B., and Subhasha, N.Multifaceted applications of isolated microalgae Chlamydomonas sp. TRC-1 in wastewater remediation, lipid production and bioelectricity generation. Bioresource Technology, 304: 122993, 2020

Shaikh, K. M., Nesamma, A. A., Abdin, M.Z., and P.P. Jutur. Molecular profiling of an oleaginous trebouxiophycean Alga Parachlorella kessleri subjected to nutrient deprivation for enhanced biofuel production. Biotechnology for Biofuels, 12: 182, 2019

Akanksha, A., Shaikh, K. M., Krushna, G., Jutur, P. P., Reena A. P., Lali, A. M. New insights under high light in mixotrophic alga Asteracys sp. to understand the regigging of molecules for production of biomass – A metabolomic approach. Algal Research, 43: 101646, 2019

Chetan, P., Asha A. N., and P. P. Jutur. Industrial scope with high-value biomolecules from microalgae. In Sustainable Downstream Processing of Microalgae for Industrial Applications, edited by Gayen K., Bhowmick T., Maity S., CRC Press, Boca Raton, USA, pp. 83-98, November 2019

Chetan, P., Tonmoy, G., Asha A. N., and P. P. Jutur. Functional omics and big data analysis in microalgae: The repertoire of molecular tools in algal technologies. In Algal Technologies and Phytochemicals, Volume I – Food, Health and Nutraceutical Applications, edited by G. A. Ravishankar, R. R. Ambati, CRC Press, Boca Raton, USA, pp. 261-271, July 2019

Vikas, U. K., Asha, A.N., and P.P. Jutur. Characterization of OMEGA fatty acids in Microalgae: A Gene Mining and Phylogenomics Approach. Preparative Biochemistry and Biotechnology, 48: 619-62, 2018

Kashif, M.S., Asha, A.N., Abdin, M.Z., and P.P. Jutur. Evaluation of growth and lipid profiles in six different microalgal strains for biofuel production. In Recent Advances in Bioenergy Research, Proceedings of the Second International Conference. edited by Kumar S, et al. Springer Singapore; pp. 3-16, 2018

Ashwani, K.R., Asha, A.N., and P.P. Jutur. Stress biology in microalgae depicts molecular insights for simultaneous production of lipids and high value precursors. Advances in Biotechnology and Microbiology, 6(5): 555699, 2017

Sardar, R., Kashif, M.S., and P.P. Jutur. Identification of transcription hubs that control lipid metabolism and carbon concentrating mechanism in model microalgae Chlamydomonas reinhardtii using regulatory networks. IEEE – International Conference on Bioinformatics and Systems Biology (BSB), Allahabad, pp. 1-4, 2016

Jutur. P.P., Asha, A.N., and M.S. Kashif. Algae-derived marine oligosaccharides and their biological applications. Frontiers in Marine Science 3(83): 1-5, 2016

Asha, A.N., Kashif, M.S., and P.P. Jutur. De novo transcriptomics in marine microalgae: an advanced genetic engineering approach for next-generation renewable biofuels. In Marine OMICS: Principles and Applications. edited by S.K. Kim: CRC Press, pp. 283-296, August 2016

Singh, R., Mattam, A.J., Jutur, P.P., and S.S. Yazdani. Synthetic biology in biofuels production. In Reviews in Cell Biology and Molecular Medicine. 2: 144-176, 2016

Jutur, P.P., and A.N. Asha. Marine Microalgae: Exploring the systems through an omics approach for biofuel production. In Marine Bioenergy: Trends and Developments. edited by S.K. Kim and C.G. Lee: CRC Press, pp. 149-162, 2015

Jutur, P.P., and A.N. Asha. Genetic engineering of marine microalgae to optimize bioenergy production. In Handbook of Marine Microalgae: Biotechnology Advances. edited by S.K. Kim: Elsevier Science, pp. 371-381, 2015

Asha, A.N., Kashif, M.S., and P.P. Jutur. Genetic engineering of microalgae for production of value-added ingredients. In Handbook of Marine Microalgae: Biotechnology Advances. edited by S.K. Kim: Elsevier Science, pp. 405-414, 2015

Jutur, P.P., and A.R. Reddy. Isolation, purification and properties of new restriction endonucleases from Bacillus badius and Bacillus lentus. Microbiological Research 162: 378-383, 2007

Jutur, P.P., and A.N. Asha. Microarrays and their applications – a versatile tool in understanding gene expression profiling. Bioinformatics Trends 1: 69-80, 2007

Sumithra, K., Jutur, P.P., Dalton, B.C., and A.R. Reddy.  Salinity-induced changes in two cultivars of Vigna radiata:  responses of antioxidative and proline metabolism. Plant Growth Regulation 50: 11-22, 2006

Desingh, R., Jutur, P.P., and A.R. Reddy. Salinity stress-induced changes in photosynthesis and antioxidative systems in three Casuarina species. Journal of Plant Biology 33: 155-161, 2006

Reddy, A.R., Chaitanya, K.V., Jutur, P.P., and A. Gnanam.  Photosynthesis   and oxidative stress   responses   to   water   deficit   in   five   different   mulberry (Morus alba.  L)  cultivars. Physiology and Molecular Biology of Plants 11: 291-298, 2005

Jutur, P.P., and A. R. Reddy.  BpaI and BpnI:  Type II restriction endonucleases from Bacillus pasteurii and Bacillus pantothenticus. Biotechnology Letters 26: 929-932, 2004

Jutur, P.P., Hoti, S.L., and A.R. Reddy. Bsu2413I and Bfi2411I, two new thermophilic type II restriction endonucleases from Bacillus subtilis and Bacillus firmus:  Isolation and   partial purification. Molecular Biology Reports 31:139-142, 2004

Swarnalatha, M., Jutur, P.P., Amouda, V., Mathur, P.P., and A.R. Reddy. Prediction of transcriptional regulation by Dlx and Msx homeodomain proteins. Online Journal of Bioinformatics 5: 132-145, 2004

Reddy, A.R., Chaitanya, K.V., and P.P. Jutur. Photosynthesis and drought adaptive characteristics in eight open pollinated families of E. camaldulensis Dehn and E. tereticornis Sm. In Tree Improvement and Biotechnology. edited by P. Shanmugavel and S. Ignachimuthu, Pointer publications, India, pp. 130-140, 2004

Reddy, A.R., Chaitanya, K.V., Jutur, P.P., and K. Sumithra. Differential anti oxidative responses to water stress among five Mulberry (Morus alba L.) cultivars. Environmental and Experimental Botany 52: 33-42, 2004

Sundar, D., Chaitanya, K.V., Jutur, P.P., and A.R. Reddy. Low temperature-induced changes in antioxidant metabolism in rubber-producing shrub, guayule (Parthenium argentatum    Gray). Plant Growth Regulation 44: 175-181, 2004

Chaitanya, K.V., Jutur, P.P., Sundar, D., and A.R. Reddy. Water stress effects on photosynthesis in different mulberry cultivars. Plant Growth Regulation 40: 75-80, 2003

Chaitanya, K.V., Jutur, P.P., Masilamani, S., and A.R. Reddy. Biomass yields in relation to photosynthesis in four different cultivars of mulberry. Ind. J. of Plant Physiology 8: 158-163, 2003

Jutur, P.P., and A.R. Reddy. A novel restriction endonuclease Bsu121I from Bacillus subtilis. Journal of Mycopathological Research 41: 247-250, 2003

Jutur, P.P., and A.R. Reddy. Bioinformatics: an overview of its molecular tools in a user’s perspective. Bioinformatics India 1: 13-22, 2003

Jutur, P.P., Chaitanya, K.V., and A.R. Reddy. Proteomics: an overview in developmental biology. Bioinformatics India 1: 65-73, 2003

Jutur, P.P., and A.R. Reddy.  Isolation and partial purification of novel restriction endonuclease Bsu121I, from Bacillus subtilis. Molecular Biology Reports 29: 383-385, 2002 Chaitanya, K.V., Masilamani, S., Jutur, P.P., and A.R. Reddy. Variation in photosynthetic rates and biomass productivity among four Mulberry cultivars. Photosynthetica 40: 305-308, 2002