Group Leader, Omics of Algae
International Centre for Genetic Engineering and Biotechnology
Aruna Asaf Ali Marg 110 067 New Delhi, India
E-mail: [email protected]; [email protected]
Office tel: +91-11-26741358 ext 451 Office fax: +91-11-26742316
Pondicherry University, Pondicherry, India, PhD, 2005
Pondicherry University, Pondicherry, India, MPhil, 2001
Kuvempu University, Davanagere, Karnataka, India, MSc, 1999
Since 2017, Group Leader, Omics of Algae Group, Integrative Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
2012-2017, Team Leader, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
2009-2012, Postdoctoral Research Associate, University of Nebraska, Lincoln, USA
2008-2009, Lecturer, BITS-Pilani (Goa Campus), Goa, India
2006-2008, ISSAR Pharma Pvt Ltd/USP India Pvt Ltd, Hyderabad, India
2005-2006, Research Associate, University of Hyderabad, Hyderabad, India
Decreasing fossil fuels and its impact on global warming have led to an increasing demand for its replacement by sustainable renewable biofuels. Microalgae may offer a potential feedstock for renewable biofuels capable of converting atmospheric CO2 to substantial biomass and valuable biofuels, which is of great importance for the food and energy industries. Parachlorella kessleri, a marine unicellular green alga belonging to class Trebouxiophyceae, accumulates large amount of lipids under nutrient-deprived conditions. The present study aims to understand the metabolic imprints in order to elucidate the physiological mechanisms of lipid accumulations in this microalga under nutrient deprivation. Molecular profiles were obtained using gas chromatography-mass spectrometry (GC-MS) of P. kessleri subjected to nutrient deprivation. Relative quantities of more than 60 metabolites were systematically compared in all the three starvation conditions. Our results demonstrate that in lipid metabolism, the quantities of neutral lipids increased significantly followed by the decrease in other metabolites involved in photosynthesis, and nitrogen assimilation. Nitrogen starvation seems to trigger the triacylglycerol (TAG) accumulation rapidly, while the microalga seems to tolerate phosphorous limitation, hence increasing both biomass and lipid content. The metabolomic and lipidomic profiles have identified a few common metabolites such as citric acid and 2-ketoglutaric acid which play significant role in diverting flux towards acetyl-CoA leading to accumulation of neutral lipids, whereas other molecules such as trehalose involve in cell growth regulation, when subjected to nutrient deprivation. Understanding the entire system through qualitative (untargeted) metabolome approach in P. kessleri has led to identification of relevant metabolites involved in the biosynthesis and degradation of precursor molecules that may have potential for biofuel production, aiming towards the vision of tomorrow’s bioenergy needs.
Photosynthetic organisms ﬁx inorganic carbon through carbon capture machinery (CCM) that regulates the assimilation and accumulation of carbon around ribulose-1,5bisphosphate carboxylase/oxygenase (Rubisco). However, few constraints that govern the central carbon metabolism are regulated by the carbon capture and partitioning machinery. In order to divert the cellular metabolism toward lipids and/or biorenewables it is important to investigate and understand the molecular mechanisms of the CO2 -driven carbon partitioning. In this context, strategies for enhancement of CO2 ﬁxation which will increase the overall biomass and lipid yields, can provide clues on understanding the carbon assimilation pathway, and may lead to new targets for genetic engineering in microalgae. In the present study, we have focused on the physiological and metabolomic response occurring within marine oleaginous microalgae Microchloropsis gaditana NIES 2587, under the inﬂuence of very-low CO2 (VLC; 300 ppm, or 0.03%) and high CO2 (HC; 30,000 ppm, or 3% v/v). Our results demonstrate that HC supplementation in M. gaditana channelizes the carbon ﬂux toward the production of long chain polyunsaturated fatty acids (LC-PUFAs) and also increases the overall biomass productivities (up to 2.0-fold). Also, the qualitative metabolomics has identiﬁed nearly 31 essential metabolites, among which there is a signiﬁcant fold change observed in accumulation of sugars and alcohols such as galactose and phytol in VLC as compared to HC. In conclusion, our focus is to understand the entire carbon partitioning and metabolic regulation within these photosynthetic cell factories, which will be further evaluated through multiomics approach for enhanced productivities of biomass, biofuels, and bioproducts (B3).
The economic feasibility of the next-generation algal biofuels relies largely on the simultaneous production of high-value-added bioproducts (HVABs) in these photosynthetic cell factories. In this study, a newly isolated indigenous microalga belonging to the selenastraceae family Monoraphidium sp. (designated as CABeR41) has been isolated and characterized for its relevant biofuel precursors along with other HVABs, i.e., tocopherols. Our preliminary data sets demonstrate accumulation of higher amounts of lipids (266.6 mg g−1 dcw) along with antioxidant molecules, such as α- and δ-tocopherols (456 µg g−1 dcw and 974 µg g−1 dcw), simultaneously when subjected to nutrient deprivation. The total tocopherol content has been increased in nitrogen-deplete conditions to 1450.24 µg g−1 dcw from 638.2 µg g−1 dcw. In conclusion, bioprospecting of new isolates from natural habitats and ﬁne-tuning of speciﬁc conditions without compromising growth has potential for developing into a sustainable industrial strain for concomitant production of biofuels and bioproducts.
The need for mass production of biofuels has been pushed up by rising energy demand in the world population. In this context, microalgae have emerged as a potential source for biodiesel production because of its high lipid content and ability to grow on minimal media sources. However, the knowledge of the metabolic processes in these systems is fairly naive and therefore hinders the genetic progress that can be proven to improve the development of biofuel precursors. In the present study, a comprehensive analysis of an indigenous oleaginous microalgae P. kessleri has been performed, using hybrid assembly of both short Illumina and long Nanopore read, leading to assembly of a highly contiguous 64.90 Mb genome with 8,371 functional genes and 874 putative transcription factors. The genome reconstruction is complemented using phylogenetic analysis of acyl lipid metabolism revealed the evolutionary conservation of these proteins among various microalgal species. Henceforth, understanding and reconstruction of global metabolic pathways will provide clues towards engineering of algal strains for enhanced production of biomass, biofuels and bioproducts (B3).
Aurantiochytrium sp. is a marine thraustochytrid that accumulates high levels of docosahexaenoic acid, a long-chain polyunsaturated fatty acid essential for cellular components. In the present study, the impact of different carbon substrates on the productivity of docosahexaenoic acid in the native marine isolate has been investigated to understand metabolomic changes within the strain. Our preliminary data demonstrates that overall biomass and docosahexaenoic acid productivities were maximal at 3% (w/v) glycerol concentration, i.e., 1.91 g L-1 D-1, and 134.96 mg L-1 D-1, respectively. Further investigations were done using qualitative metabolomics to identify metabolites that delineate their physiological changes under specific conditions. Nearly 34 metabolites comprising of fatty acids, sugars, and organic acids were found to be significantly regulated, thus underlining the importance of these functional metabolites in the polyunsaturated fatty acid biosynthesis i.e., Omega. This led to our hypothesis that utilization of carbon substrate such as glycerol is able to induce cell growth along with the production of these essential fatty acids via., upregulation of pentose phosphate pathway. In conclusion, this study provides us an alternative and cost-effective carbon source for the production of high-value added omega’s employing valorization of by-products (e.g., glycerol) from biorefineries in heterotrophic thraustochytrids.
The major concern among microalgae genetic and metabolic engineering is lack of molecular tools and overall poor expression of heterologous genes from the nuclear genome of many microalgae species, at least partially due to their rapid silencing. Tools for synthetic biology and genetic engineering in algae are yet to be developed or still infancy. Our future perspectives will be development of basic interchangeable elements for regulating the metabolic pathways such as common promoters, transcriptional terminators, ribosome binding sites, and other regulatory factors in microalgae. The following strategies will be employed on the fundamentals, and engineering process of microalgae together with global R&D efforts and attempts, thus further aiming towards commercialization.
- Understanding the overall regulatory genes in TAG biosynthesis and catabolism pathways, oil mobilization and also gene silencing altering the lipid content to increase in overall lipid production or shift the balance of lipid production without comprising growth.
- Studies are underway for determining the role played by chemical molecules having any effect on microalgae metabolism i.e., growth and/or lipid biosynthesis.
- Integrated Omics and Big Data approach to understanding the complex mechanisms in microalgae for biofuel production.
- Development of genome editing tools for genetic engineering in microalgae.
- Identification of high-value renewables from microalgae such as OMEGAs, Lutein, Astaxanthin, natural pigments etc. of industrial relevance and scale-up.
Microalgal growth can be better understood by the interaction of algal biofuel production with the environment through development of models describing their usefulness for designing efficient bioreactor, predicting process performance and optimizing operating conditions. Models for life cycle assessment (LCA) provide information about technology, economics, and sustainability along with evaluation of the inputs and outputs and their potential environmental impacts of a product, and the final outcome may vary with different culture systems, and the different methods used for biomass harvest and oil refinery.
Our major futuristic improvements will be focused for economical microalgal fuel production:
- Improving photosynthetic efficiency through genetic/metabolic engineering in microalgal mass culturing along with economical harvesting procedures.
- Channeling of fixed carbon into higher fuel value products of commercial relevance.
- Development of robust, ‘engineered’ algal cells that will persist and compete in lower-cost open or semi-open environments.
These insights will provide solutions for some of major ongoing concerns like high capital investments, operational costs and as well as contamination problems in microalgal biofuel production.
Jutur, P.P., Kumar, A., Rehmanji, M., Singh, R. and Nesamma, A.A. In silico genome assembly and functional annotation of algae. In Methods in Cyanobacterial Research. Singh, P. S. (ed), 2022 (in press)
Singh, R., Kuberwa, A. M., Nesamma, A. A. and P.P. Jutur. iOMICS: New paradigm in algal biotechnology. In Genetics and Genomics of Algae, Thangadurai, D. (ed), AAP/CRC Press, USA and Springer Nature, Switzerland, 2022 (in press)
Singh, R., Paliwal, C., Rehmanji, M., Nesamma, A. A. and P.P. Jutur. Biomass valourization: A new perspective in the algal-based sustainable biorefineries. In Prospect and Potential of Algal Biomass, Ghosh, D. & Tiwari, O. N. (eds), Apple Academic Press (Taylor & Francis Group), UK, 2022 (in press)
Makaranga, A., and P.P. Jutur. Algae-bacteria interactomics unveils their role in growth and production of high-value biorenewables. In Micro-algae: Next-generation Feedstock for Biorefineries. Clean Energy Production Technologies, Verma, P. (ed), Springer, Singapore, pp. 165-176, 2022. doi: 10.1007/978-981-19-0680-0_8
Zafar, U, S., Mehra, A., and P.P. Jutur. Synthetic biology based advanced biotechnological approach in micro-algal biorefinery. In Micro-algae: Next-generation Feedstock for Biorefineries. Clean Energy Production Technologies, Verma, P. (ed), Springer, Singapore, pp. 205-230, 2022. doi: 10.1007/978-981-19-0680-0_10.
Singh, R., Rehmanji, M., Nesamma, A. A., and P.P. Jutur.Algal cell factories as a source of marine antioxidants. In Marine Antioxidants – Preparations, Syntheses, and Applications, edited by Kim, S-K., Shin, K-H., Venkatesan, J. (eds), Elsevier Publishers Inc., USA, 2022 (in press)
Naira, V. R., Kareya, M. S., Nesamma, A. A., and P.P. Jutur. Mass spectrometry. In Handbook of Biomolecules: Fundamentals, Properties and Applications, edited by Verma, C., Verma, D. K. (eds), Elsevier Publishers Inc., USA, 2022 (in press).
Makaranga, A., Shaikh, K. M., Nesamma, A. A., and P.P. Jutur. Bioremediation by Microalgae: Current Progress and Future Perspectives. In Sustainable Materials: Processes, Technologies and Applications, edited by Inamuddin, Bentham Science Publishers, 2022 (in press).
Paliwal, C., Rehmanji, M., Shaikh, K. M., Zafar, S. U., and P.P. Jutur. Green extraction processing of hydrophobic value-added carotenoids in water-base ionic liquids as a sustainable innovation in algal biorefineries. Algal Research, 66: 102809, 2022 [VSI: ‘Innovations in algal farming and value chain development; Guest Editors: Drs. CRK Reddy, Ana Otero and Pascale Champagne’] doi: 10.1016/j.algal.2022.102809 [IF: 5.276]
Mehra, A., Zafar, S. U., and P.P. Jutur.Optimization of Biomass production by Chlorella saccharophila UTEX 247 employing response surface methodology. Biomass Conversion and Biorefinery, 2022. doi: 10.1007/s13399-022-02966-4 [IF: 4.05]
Rehmanji, M., Nesamma, A., Khan, N., Fatma, T., and P.P. Jutur. Media engineering in marine diatom Phaeodactylum tricornutum employing cost-effective substrates for sustainable production of high value renewables. Biotechnology Journal, e2100684, 2022. doi: 10.1002/biot.202100684 [IF: 5.726]
Kareya, M. S., Mariam, I., Rajacharya, G. H., Nesamma, A. A., and Jutur, P. P. Valorization of carbon dioxide (CO2) to enhance production of biomass, biofuels and biorenewables (B3) in Chlorella saccharophila UTEX247: A circular bioeconomy perspective. Biofuels, Bioproducts & Biorefining, 16: 682-697, 2022. doi: 10.1002/BBB.2295 [IF: 5.239]
Mehra, A., and P.P. Jutur.Application of response surface methodology (RSM) for optimizing biomass production in Nannochloropsis oculata UTEX 2164. Journal of Applied Phycology, 34: 1893-1907, 2022. doi: 10.1007/ s10811-022-02774-3 [IF: 3.404]
Singh, R., Nesamma, A. A., Narula, A., and P.P. Jutur. Multi-fold enhancement of tocopherol yields employing high CO2 supplementation and nitrate limitation in Monoraphidium sp. Cells, 11: 1315, 2022 [Special Issue “Growth and Division in Algae”, Academic Editor: Kateřina Bišová], doi: 10.3390/cells11081315 [IF: 7.666]
Rehmanji, M., Suresh, S., Nesamma, A.A., and P.P. Jutur. Microalgae: A multifaceted treasure of pharmaceuticals and nutraceuticals. In Algal Genetic Resources: Cosmeceuticals, Nutraceuticals and Pharmaceuticals, Shivasharana C. T., Devarajan T., Jeyabalan S. (eds), AAP/CRC Press, USA, pp. 211-266, 2022. doi: 10.1201/9781003277095
Nesamma, A. A., Mariam, I., Kareya, M. S., and P.P. Jutur. Application of microalgae for CO2 mitigation and sequestration of flue gas. In Algal Biorefineries and the Circular Bioeconomy, Volume 2: Industrial Applications and Future Prospects, edited by Sanjeet Mehariya, Shahsi Bhatia and Obulisamy Parthiba Karthikeyan, CRC Press | Taylor & Francis Group, USA, pp. 93-114, 2022. doi: 10.1201/9781003188094-3.
Kareya, M.S., Mehra, A., Nesamma, A.A., and Jutur, P.P. Valorisation of macroalgal biomass for sustainable biorefineries. In Sustainable Global Resources of Seaweeds Volume 1: Bioresources, cultivation, trade and multifarious applications, edited by Ranga Rao A. and Ravishankar G.A., Springer International Publishing, Cham, pp. 603-626, 2022. doi: 10.1007/978-3-030-91955-9_32.
Rehmanji, M., Singh, R., Nesamma, A. A., Fatma, T., Khan, N.J., Narula, A., and P.P. Jutur. Multifaceted applications of microalgal biomass valorization to enriched biorenewables, a review of futuristic biorefinery paradigm. Bioresource Technology Reports, 17: 100972, 2022. doi: 10.1016/j.biteb.2022.100972.
Mariam, I., Kareya, M, S., Rehmanji, M., Nesamma, A. A., and Jutur, P. P. Channelling of carbon flux towards carotenogenesis in Botryococcus braunii: A media engineering perspective. Frontiers in Microbiology, 12: 693106, 2021. doi: 10.3389/fmicb.2021.693106 [IF: 6.064]
Ghosh, T., Singh, R., Nesamma, A. A., and P.P. Jutur. Marine Polysaccharides: Properties and Applications. In Polysaccharides: Properties and Applications, edited by Inamuddin, M. I. Ahamed, R. Boddula, T. A. Altalhi, Scrivener Publishing LLC., USA, pp. 37-60, 2021. doi: 10.1002/9781119711414.ch3.
Mariam, I., Kareya, M, S., Nesamma, A. A., and P.P. Jutur. Delineating metabolomic changes in native isolate Aurantiochytrium for production of docosahexaenoic acid in presence of varying carbon substrates. Algal Research, 55: 102285, 2021. doi: 10.1016/j.algal.2021.102285 [IF: 5.276]
Paliwal, C., and P. P. Jutur. Dynamic allocation of carbon flux triggered by task-specific chemicals is an effective non-gene disruptive strategy for sustainable and cost-effective algal biorefineries. Chemical Engineering Journal, 418: 129413, 2021. doi: 10.1016/j.cej.2021.129413 [IF: 16.744]
Paliwal, C., Kareya, M. S., Singh, R., Nesamma, A.A., and P.P. Jutur. Integrated omics perspective to understand the production of high-value added biomolecules (HVABs) in microalgal cell factories. In Microbial Cell Factories Engineering for Production of Biomolecules, edited by Vijai Singh, Elsevier Publishers Inc., USA, pp. 303-317, 16th February 2021. doi: 10.1016/B978-0-12-821477-0.00020-9.
Rehmanji, M., Suresh, S., Nesamma, A.A., and P.P. Jutur. Microalgal cell factories, a platform for high-value added biorenewables to improve economics of the biorefinery. In Microbial and Natural Macromolecules, edited by Surajit Das, Hirak Dash, Elsevier Publishers Inc., USA, pp. 689-731, 2021. doi: 10.1016/B978-0-12-820084-1.00027-2.
Shaikh, K.M., Kumar, P., Nesamma, A. A., Abdin, M.Z., and P.P. Jutur. Hybrid genome assembly and functional annotation reveals insights on lipid biosynthesis of oleaginous native isolate Parachlorella kessleri, a potential industrial strain for production of biofuel precursors. Algal Research, 52: 102118, 2020. doi: 10.1016/j.algal.2020.102118 [IF: 5.276]
Singh, R., Paliwal, C., Nesamma, A. A., Narula, A., and P.P. Jutur. Nutrient deprivation mobilizes the production of unique tocopherols as a stress-promoting response in a new indigenous isolate Monoraphidium sp. Frontiers in Marine Science, 7: 575817, 2020. doi: 10.3389/fmars.2020. 575817 [IF: 5.247]
Kareya, M. S., Mariam, I., Shaikh, K. M., Nesamma, A. A., and P.P. Jutur. Photosynthetic carbon partitioning and metabolic regulation in response to very-low and high CO2 in M. gaditana NIES 2587. Frontiers in Plant Science, 11: 981, 2020. doi: 10.3389/fpls.2020.00981 [IF: 6.627]
Kumari, S., Nesamma, A. A., Lali, A. M., Jutur, P. P., and G. Prakash. The chloroplast genome of a resilient chlorophycean microalga Asterarcys sp. Algal Research, 49: 101952, 2020. doi: 10.1016/j.algal.2020.101952[IF: 5.276]
Shaikh, K. M., Mariam, I., Nesamma, A.A., Abdin, M.Z., and P.P. Jutur. Application of transgenic technologies in biofuel production through photosynthetic chassis – new paradigms from gene mining to genome editing. In Transgenic Technology Based Value addition in Plant Biotechnology, edited by M. Z. Abdin, Usha Kiran, Elsevier Publishers Inc., USA, pp. 227-245, July 2020. doi: 10.1016/B978-0-12-818632-9.00010-1.
Kareya, M. S., Mariam, I., Nesamma, A. A., and P.P. Jutur. CO2 sequestration by hybrid integrative photosynthesis (CO2SHIP) – A green initiative for multi-product biorefineries. Materials Science for Energy Technologies, 3: 420-428, 2020. doi: 10.1016/j.mset.2020.03.002.
Kannikka, B., Pasupuleti, S. C., Monika, J., Mahima, S., Ashish, M., Kareya, M. S., Jutur, P. P., Amit, B., and Subhasha, N.Multifaceted applications of isolated microalgae Chlamydomonas sp. TRC-1 in wastewater remediation, lipid production and bioelectricity generation. Bioresource Technology, 304: 122993, 2020. doi: 10.1016/j.biortech.2020.122993 [IF: 11.846]
Shaikh, K. M., Nesamma, A. A., Abdin, M.Z., and P.P. Jutur. Molecular profiling of an oleaginous trebouxiophycean Alga Parachlorella kessleri subjected to nutrient deprivation for enhanced biofuel production. Biotechnology for Biofuels, 12: 182, 2019. doi: 10.1186/s13068-019-1521-9 [IF: 7.67]
Akanksha, A., Shaikh, K. M., Krushna, G., Jutur, P. P., Reena A. P., Lali, A. M. New insights under high light in mixotrophic alga Asteracys sp. to understand the regigging of molecules for production of biomass – A metabolomic approach. Algal Research, 43: 101646, 2019. doi: 10.1016/j.algal.2019.101646 [IF: 5.276]
Paliwal, C., Nesamma, A.A., and P. P. Jutur. Industrial scope with high-value biomolecules from microalgae. In Sustainable Downstream Processing of Microalgae for Industrial Applications, edited by Gayen K., Bhowmick T., Maity S., CRC Press, Boca Raton, USA, pp. 83-98, November 2019. doi: 10.1201/9780429027970.
Paliwal, C., Ghosh, T., Nesamma, A.A., and P. P. Jutur. Functional omics and big data analysis in microalgae: The repertoire of molecular tools in algal technologies. In Algal Technologies and Phytochemicals, Volume I – Food, Health and Nutraceutical Applications, edited by G. A. Ravishankar, R. R. Ambati, CRC Press, Boca Raton, USA, pp. 261-271, July 2019. doi: 10.1201/9780429054242.
Kapase, V.U., Nesamma, A.A., and P.P. Jutur. Characterization of OMEGA fatty acids in microalgae: A gene mining and phylogenomics approach. Preparative Biochemistry and Biotechnology, 48: 619-62, 2018. doi: 10.1080/10826068.2018.1476886 [IF: 3.141]
Shaikh, K. M., Nesamma, A.A., Abdin, M.Z., and P.P. Jutur. Evaluation of growth and lipid profiles in six different microalgal strains for biofuel production. In Recent Advances in Bioenergy Research, Proceedings of the Second International Conference. edited by Kumar S, et al. Springer Singapore; pp. 3-16, 2018. doi: 10.1007/978-981-10-6107-3_1.
Rai, A.K., Nesamma, A.A., and P.P. Jutur. Stress biology in microalgae depicts molecular insights for simultaneous production of lipids and high value precursors. Advances in Biotechnology and Microbiology, 6(5): 555699, 2017. doi: 10.19080/AIBM.2017.06.555699 [IF: 1.023]
Sardar, R., Shaikh, K. M., and P.P. Jutur. Identification of transcription hubs that control lipid metabolism and carbon concentrating mechanism in model microalgae Chlamydomonas reinhardtii using regulatory networks. IEEE – International Conference on Bioinformatics and Systems Biology (BSB), Allahabad, pp. 1-4, 2016. doi: 10.1109/BSB.2016.7552116.
Jutur. P.P., Nesamma, A.A., and K. M. Shaikh. Algae-derived marine oligosaccharides and their biological applications. Frontiers in Marine Science 3: 83, 2016. doi: 10.3389/fmars. 2016.00083 [IF: 5.247]
Nesamma, A.A., Shaikh, K.M., and P.P. Jutur. De novo transcriptomics in marine microalgae: an advanced genetic engineering approach for next-generation renewable biofuels. In Marine OMICS: Principles and Applications. edited by S.K. Kim: CRC Press, pp. 283-296, August 2016. doi: 10.1201/9781315372303-16.
Singh, R., Mattam, A.J., Jutur, P.P., and S.S. Yazdani. Synthetic biology in biofuels production. In Reviews in Cell Biology and Molecular Medicine. 2: 144-176, 2016. doi: 10.1002/3527600906.mcb.201600003.
Jutur, P.P., and A.A. Nesamma. Marine Microalgae: Exploring the systems through an omics approach for biofuel production. In Marine Bioenergy: Trends and Developments. edited by S.K. Kim and C.G. Lee: CRC Press, pp. 149-162, 2015. doi: 10.1201/b18494-12.
Jutur, P.P., and A.A. Nesamma. Genetic engineering of marine microalgae to optimize bioenergy production. In Handbook of Marine Microalgae: Biotechnology Advances. edited by S.K. Kim: Elsevier Science, pp. 371-381, 2015. doi: 10.1016/B978-0-12-800776-1.00024-8.
Nesamma, A.A., Shaikh, K. M., and P.P. Jutur. Genetic engineering of microalgae for production of value-added ingredients. In Handbook of Marine Microalgae: Biotechnology Advances. edited by S.K. Kim: Elsevier Science, pp. 405-414, 2015. doi: 10.1016/B978-0-12-800776-1.00026-1.
Jutur, P.P., and A.R. Reddy. Isolation, purification and properties of new restriction endonucleases from Bacillus badius and Bacillus lentus. Microbiological Research 162: 378-383, 2007. doi: 10.1016/j.micres. 2006.01.008 [IF: 5.07]
Jutur, P.P., and A.A. Nesamma. Microarrays and their applications – an versatile tool in understanding gene expression profiling. Bioinformatics Trends 1: 69-80, 2007.
Sumithra, K., Jutur, P.P., Dalton, B.C., and A.R. Reddy. Salinity-induced changes in two cultivars of Vigna radiata: responses of antioxidative and proline metabolism. Plant Growth Regulation 50: 11-22, 2006. doi: 10.1007/s10725-006-9121-7 [IF: 3.242]
Desingh, R., Jutur, P.P., and A.R. Reddy. Salinity stress-induced changes in photosynthesis and antioxidative systems in three Casuarina species. Journal of Plant Biology 33: 155-161, 2006 [IF: 3.15]
Reddy, A.R., Chaitanya, K.V., Jutur, P.P., and A. Gnanam. Photosynthesis and oxidative stress responses to water deficit in five different mulberry (Morus alba. L) cultivars. Physiology and Molecular Biology of Plants 11: 291-298, 2005.
Jutur, P.P., and A. R. Reddy. BpaI and BpnI: Type II restriction endonucleases from Bacillus pasteurii and Bacillus pantothenticus. Biotechnology Letters 26: 929-932, 2004. doi: 10.1023/b: bile.0000025905.14101.39 [IF: 2.716]
Jutur, P.P., Hoti, S.L., and A.R. Reddy. Bsu2413I and Bfi2411I, two new thermophilic type II restriction endonucleases from Bacillus subtilis and Bacillus firmus: Isolation and partial purification. Molecular Biology Reports 31:139-142, 2004. doi: 10.1023/b:mole.0000031503.19851.3b [IF: 2.742]
Swarnalatha, M., Jutur, P.P., Amouda, V., Mathur, P.P., and A.R. Reddy. Prediction of transcriptional regulation by Dlx and Msx homeodomain proteins. Online Journal of Bioinformatics 5: 132-145, 2004.
Reddy, A.R., Chaitanya, K.V., and P.P. Jutur. Photosynthesis and drought adaptive characteristics in eight open pollinated families of E. camaldulensis Dehn and E. tereticornis Sm. In Tree Improvement and Biotechnology. edited by P. Shanmugavel and S. Ignachimuthu, Pointer publications, India, pp. 130-140, 2004.
Reddy, A.R., Chaitanya, K.V., Jutur, P.P., and K. Sumithra. Differential anti oxidative responses to water stress among five Mulberry (Morus alba L.) cultivars. Environmental and Experimental Botany 52: 33-42, 2004. doi: 10.1016/j.envexpbot.2004.01.002 [IF: 6.028]
Sundar, D., Chaitanya, K.V., Jutur, P.P., and A.R. Reddy. Low temperature-induced changes in antioxidant metabolism in rubber-producing shrub, guayule (Parthenium argentatum Gray). Plant Growth Regulation 44: 175-181, 2004. doi: 10.1007/s10725-004-4030-0 [IF: 3.242]
Chaitanya, K.V., Jutur, P.P., Sundar, D., and A.R. Reddy. Water stress effects on photosynthesis in different mulberry cultivars. Plant Growth Regulation 40: 75-80, 2003. doi: 10.1023/a:10230 64328384 [IF: 3.242]
Chaitanya, K.V., Jutur, P.P., Masilamani, S., and A.R. Reddy. Biomass yields in relation to photosynthesis in four different cultivars of mulberry. Ind. J. of Plant Physiology 8: 158-163, 2003.
Jutur, P.P., and A.R. Reddy. A novel restriction endonuclease Bsu121I from Bacillus subtilis. Journal of Mycopathological Research 41: 247-250, 2003.
Jutur, P.P., and A.R. Reddy. Bioinformatics: an overview of its molecular tools in a user’s perspective. Bioinformatics India 1: 13-22, 2003.
Jutur, P.P., Chaitanya, K.V., and A.R. Reddy. Proteomics: an overview in developmental biology. Bioinformatics India 1: 65-73, 2003.
Jutur, P.P., and A.R. Reddy. Isolation and partial purification of novel restriction endonuclease Bsu121I, from Bacillus subtilis. Molecular Biology Reports 29: 383-385, 2002. doi: 10.1023/a:1021260803765 [IF: 2.742]
Chaitanya, K.V., Masilamani, S., Jutur, P.P., and A.R. Reddy. Variation in photosynthetic rates and biomass productivity among four Mulberry cultivars. Photosynthetica 40: 305-308, 2002. doi: 10.1023/a:1021318412861 [IF: 2.482]