http://www.icgeb.org Studies in the function of genes and their mutations. Feeder 3 3.7.5(3340) Mac App Store Version; Mac OS X Version 10.16 (Build 21D62) http://reinventedsoftware.com/feeder/ http://blogs.law.harvard.edu/tech/rss en License: Creative Commons BY-NC-ND Fri, 06 Oct 2017 15:03:23 +0200 Fri, 06 Oct 2017 15:03:23 +0200 ICGEB Studies in the function of genes and their mutations. Studies in the function of genes and their mutations. International Centre for Genetic Engineering and Biotechnology, Trieste, Cardiovascular, ICGEB ICGEB itunesuadmin@icgeb.org no episodic Tue, 07 Sep 2021 09:41:16 +0200 76F767F3-EC14-4FB0-973D-0684AA7ADE1C Gianluca Petris, MRC Laboratory of Molecular Biology, UK speaks on "CRISPR-Cas9 and beyond: delivery, specificity and therapy". CRISPR-Cas nucleases can be considered the most powerful technologies for genome editing. However, several difficulties may affect the success of genome editing, in particular in vivo; these include achieving efficient RNA-guided nuclease delivery, appropriate targeted genome editing, absence of off-target activity and potential immune responses (in vivo) associated with long lasting nuclease presence in target cells.I will discuss the technologies I have developed to address these challenges including i) the engineering a lentiviral vector encoding a Self-Limiting Cas9 circuit for Enhanced Specificity (SLiCES), which increases genome editing precision by controlling Cas9 levels in a programmable fashion; ii) the development of a cellular production system to generate exosome-like vesicles (VEsiCas) carrying Cas9-guide RNA ribonucleoprotein complexes, which are efficiently delivered into target cells for gene editing applications; iii) examples of microhomology-based genome engineering with potential therapeutic applications where Cas12a can be exploited more successfully than Cas9 based on the different activity of these two “blades” for genetic surgery. Icgeb,Gianluca Petris 2980 full Mon, 23 May 2016 12:00:48 +0200 5032F875-BF85-44E5-81EC-C6DB06F55FFA Tibor Hortobagyi - Department of Neuropathology, Institute of Pathology, University of Debrecen, Debrecen, HUNGARY. This seminar has been recorded by ICGEB Trieste Tibor Hortobagyi - Department of Neuropathology, Institute of Pathology, University of Debrecen, Debrecen, HUNGARY speaks on “Pathology and current molecular classification of ALS/FTD”. This seminar has been recorded by ICGEB Trieste Icgeb,Tibor Hortobagyi 2103 full Wed, 20 Apr 2016 12:00:43 +0200 2841EAF0-E486-4CDD-A140-95CD3DEACBDE Andrés Klein Andrés Klein TIGEM - Telethon Institute of Genetics and Medicine, Pozzuoli (NA) - ITALY speaks on "Identification of modifier genes in lysosomal storage disorders". This seminar has been recorded by ICGEB Trieste Andrés Klein TIGEM - Telethon Institute of Genetics and Medicine, Pozzuoli (NA) - ITALY speaks on "Identification of modifier genes in lysosomal storage disorders". This seminar has been recorded by ICGEB Trieste Icgeb 2196 full Fri, 27 Feb 2015 12:00:56 +0100 13F0C876-96F2-42CD-A935-30AEFBEFDE7C Steve Oliver Steve OLIVER, Department of Biochemistry, University of Cambridge, Cambridge - UK. This seminar has been recorded by ICGEB Trieste Steve OLIVER, Department of Biochemistry, University of Cambridge, Cambridge - UK speaks on "Making, mending, and extending the yeast metabolic model". This seminar has been recorded by ICGEB Trieste Icgeb 2554 full Fri, 31 Oct 2014 12:00:00 +0100 B79A2AEC-3BE8-4213-8A56-B66A2F50BB5A Alberto Inga Alberto Inga, Centre for Integrative Biology, CIBIO, University of Trento, Trento, ITALY. This seminar has been recorded by ICGEB Trieste Alberto Inga, Centre for Integrative Biology, CIBIO, University of Trento, Trento, ITALY speaks on "p53-directed translational control can shape and expand the universe of p53 target genes". This seminar has been recorded by ICGEB Trieste Icgeb 2419 full Fri, 22 Mar 2013 15:00:00 +0100 9CADD7FA-057A-4214-B8E1-DA44A9B8F68E Michael Breitenbach Michael Breitenbach Department of Cell Biology, University of Salzburg, Salzburg, AUSTRIA.This seminar has been recorded by ICGEB Trieste Michael Breitenbach Department of Cell Biology, University of Salzburg, Salzburg, AUSTRIA speaks on "Yeast aging models".This seminar has been recorded by ICGEB Trieste Icgeb 2961 full Fri, 15 Jun 2012 12:00:00 +0200 8E468E66-C849-4519-A765-CF51CC225B96 John Diffley J. F.X. Diffley Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, UK. This seminar has been recorded by ICGEB Trieste John F.X. Diffley Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, UK speaks on "Investigating how DNA replication initiates in eukaryotes". This seminar has been recorded by ICGEB Trieste Icgeb 3766 full Fri, 09 Mar 2012 00:00:00 +0100 5F548003-F345-46F3-9095-82961E438E8F Jean-Luc Souciet Jean-Luc Souciet, Université de Strasbourg et CNRS, UMR 7156 Génétique Moléculaire, Génomique, Microbiologie, Génomique comparative des Levures Hémiascomycètes, Institut de Botanique, Strasbourg, FRANCE. This seminar has been recorded by ICGEB Trieste Jean-Luc Souciet, Université de Strasbourg et CNRS, UMR 7156 Génétique Moléculaire, Génomique, Microbiologie, Génomique comparative des Levures Hémiascomycètes, Institut de Botanique, Strasbourg, FRANCE,speaks on "The mosaic genome of the yeast Pichia sorbitophila : genomic changes occuring after formation of a recent interspecies hybrid". This seminar has been recorded by ICGEB Trieste Icgeb 2567 full Thu, 16 Feb 2012 00:00:00 +0100 8230C390-1BBD-4370-8269-4F1BEB882C5F Anna Tramontano Anna Tramontano, Department of Physics, Sapienza University of Rome, ITALY. This seminar has been recorded by ICGEB Anna Tramontano, Department of Physics, Sapienza University of Rome, ITALY, speaks on "The surprising transcriptome". This seminar has been recorded by ICGEB Icgeb 2050 full Wed, 10 Mar 2010 12:00:00 +0100 9AE8B22D-74FA-4EC0-880F-00A3465D16C1 Christopher Pearson Christopher Pearson, Department of Genetics, The Hospital for Sick Children, Toronto, CANADA. This seminar has been recorded by ICGEB Trieste Christopher Pearson, Department of Genetics, The Hospital for Sick Children, Toronto, CANADA speaks on "Repeat instability as the basis for human diseases and as a potential target for therapy". This seminar has been recorded by ICGEB Trieste 3810 full Tue, 06 Sep 2011 12:00:00 +0200 DA767525-2ABB-4A63-BD24-8DA34CB573E7 Beatriz Llamusí Troisi Beatriz Llamusí, Troisi Dpto. Genetica, Facultad de Biología Universidad de Valencia, Burjassot Valencia, SPAIN. This seminar has been recorded by ICGEB Trieste Beatriz Llamusí Troisi, Dpto. Genetica, Facultad de Biología Universidad de Valencia, Burjassot Valencia, SPAIN speaks on "TBPH involvement in Myotonic Dystrophy 1". This seminar has been recorded by ICGEB Trieste Icgeb 1661 full