University of Siena and Istituto Italiano di Tecnologia , Siena, ITALY
Tuesday, 12 April 2022 | 12:00 noon – ICGEB Trieste, ITALY
Robotics and Haptics: a Wearable Perspetive
Host: S. Zacchigna
Caliciviruses are responsible for gastroenteritis outbreaks worldwide. We have examined how different caliciviruses interacts with stress pathways. We show that human norovirus infection represses host cell translation and induces a metabolic stress that is uncoupled from eIF2a signalling. Infection also results in a redistribution of the SG-scaffolding G3BP1 protein interactome, to prevent SG assembly. In contrast, feline calicivirus impairs SGs assembly by cleaving the scaffolding protein G3BP1. We also observed that uninfected bystander cells assembled G3BP1-positive granules, suggesting a paracrine response triggered by infection. We show that paracrine signalling from infected cells induces the formation of SG-like foci, that we named paracrine granules. They are linked to antiviral activity and exhibit specific kinetics of assembly-disassembly, and protein and RNA composition that are different from canonical SGs. We propose that this paracrine induction reflects a novel cellular defence mechanism to limit viral propagation and promote stress responses in bystander cells.
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