Cytokines and Disease

INFECTIOUS DISEASES / Parasitic Diseases

Research Interests

Immunological mechanisms of host protection in infectious and non-infectious diseases.

Description of Research

The Group investigates immunological mechanisms in experimental murine models for human diseases. Major general topics include cytokine network and regulation, lymphocyte differentiation and function, dendritic cell and macrophage activation, as well as the role of non-immunological effector cells in health/disease like smooth muscle cells, goblet cells, activated by IL-4 and IL13. Current disease models under investigations include:

Bacterial Infectious Diseases
– Tuberculosis caused by Mycobacterium tuberculosis (aerosol).
– Listeriosis caused by Listeria monocytogenes.

Parasitic Infectious Diseases
– African trypanosomiasis caused by Trypanosoma brucei/evansi/congolense.
– Cutaneous leishmaniasis caused by Leishmania major.
– Schistosomiasis (Bilharzia) caused by Schistosoma mansoni.
– Hookworm caused by Nippostrongylus brasiliensis.

Non-infectious Diseases
Allergic inflammation, induced by Ovalbumin, House Dust Mite, or Anisakis.
Colitis, chemically induced by Oxazalone.

Our research strategy is based on gain of knowledge by a loss of function approaches in knockout and knockdown animal models. This includes the generation and characterisation of novel conditional gene deficient mouse strains. Together with transcriptomic approaches, the significance of genes, factors and cells for host protection and failure thereof are uncovered and possible factors for host-directed drug targeting identified. This supports our long-term goal for the development of safe and cost-effective drug and vaccination strategies.

Recent Publications

Darby, M.G., Chetty, A., Mrjden, D., Rolot, M., Smith, K., Mackowiak, C., Sedda, D., Nyangahu, D., Jaspan, H., Toellner, K.M., Waisman, A., Quesniaux, V., Ryffel, B., Cunningham, A.F., Dewals, B.G., Brombacher, F., Horsnell, W.G.C. 2019. Pre-conception maternal helminth infection transfers via nursing long-lasting cellular immunity against helminths to offspring Sci Advance 5:eaav3058 PubMed link

Abdel Aziz, N., Nono, J.K., Mpotje, T., Brombacher, F. 2018. The Foxp3+ regulatory T-cell population requires IL-4Rα signaling to control inflammation during helminth infections. PLoS Biology 16:e2005850 PubMed link

Brombacher, T.M., De Gouveia, K.S., Cruywagen, L., Makena, N., Booley, F., Tamgue, O., Brombacher, F. 2018. Nippostrongylus brasiliensis infection leads to impaired reference memory and myeloid cell interference.Sci Rep 8, 2958 PubMed link

Brombacher, T.M., De Gouveia, K.S., Cruywagen, L., Makena, N., Booley, F., Tamgue, O., Brombacher, F. 2018. Nippostrongylus brasiliensis infection leads to impaired reference memory and myeloid cell interference. Sci Rep 8, 2958 PubMed link 

Hurdayal, R., Ndlovu, H.H., Revaz-Breton, M., Parihar, S.P., Nono, J.K., Govender, M., Brombacher, F. 2017. IL-4-producing B cells regulate T helper cell dichotomy in type 1- and type 2-controlled diseases. Proc Natl Acad Sci USA 114,  E8430-E8439 PubMed link 

Parihar, S.P., Ozturk, M., Marakalala, M.J., Loots, D.T., Hurdayal, R., Beukes, D., Van Reenen, M., Zak, D.E., Mbandi, S.K., Darboe, F., Penn-Nicholson, A., Hanekom, W.A., Leitges, M., Scriba, T.J., Guler, R., Brombacher, F. 2017. Protein kinase C-delta (PKCδ), a marker of inflammation and tuberculosis disease progression in humans, is important for optimal macrophage killing effector functions and survival in mice. Mucosal Immunol 11, 579 PubMed link