
Group Leader, Membrane Protein Biology
International Centre for Genetic Engineering and Biotechnology
Aruna Asaf Ali Marg
110 067 New Delhi, India
E-mail: [email protected]
Tel: +91-11-26741358 ext. 172
Fax: +91-11-26742316
Education
Madurai Kamarai University, Madurai, India, MSc, 1993
Madurai Kamarai University, Madurai, India, PhD, 2000
Career History
Since 2016, Group Leader, Membrane Protein Biology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB) New Delhi, India
2006-2015, Staff Research Scientist, Structural and Computational Biology Group, ICGEB, New Delhi, India
2003-2006, Assistant Research Scientist, Texas A&M University, Texas, USA
2001-2003, Post-doctoral Research Associate, Texas A&M University
2000-2001, Post-doctoral Research Associate,
1994-2000, Junior and Senior Research Fellow (CSIR-NET), Madurai Kamaraj University, Tamil Nadu, India
1993-1994, Junior Research Fellow, Anna University, Chennai, Tamil Nadu, India
Scientific Activity
In Membrane Protein Biology we try to understand a few complex biological processes by basic research, using the tools of modern biology, and attempt to translate them for Atherosclerosis, Cancer and Sepsis therapy. Our research focus is on: 1) Dimorphic and bifunctional Dehydroascorbate reductases (DHARs)-cum-Chloride Intracellular Channels (CLICs) that recycle Vitamin-C as soluble enzymes and act as ion channels while membrane inserted, which are
Selected publications
Das B.K., Kumar A., Sreekumar S.N., Ponraj K., Gadave K., Kumar S., Murali Achary V.M., Ray P., Reddy M.K., Arockiasamy A. 2021. Comparative kinetic analysis of ascorbate (Vitamin-C) recycling dehydroascorbate reductases from plants and humans. Biochem Biophys Res Commun.; 591:110-117. doi: 10.1016/j.bbrc.2021.12.103. PMID: 35007834.
Panchariya L., Khan W.A., Kuila S., Sonkar K., Sahoo S., Ghoshal A., Kumar A., Verma D.K., Hasan A., Khan M.A., Jain N., Mohapatra A.K., Das S., Thakur J.K., Maiti S., Nanda R.K., Halder R., Sunil S., Arockiasamy A. 2021. Zinc2+ ion inhibits SARS-CoV-2 main protease and viral replication in vitro. Chem Commun (Camb). doi: 10.1039/d1cc03563k
Babu, K., Arulandu, A., Sankaran, K. 2017. The structure of DLP12 endolysin exhibiting alternate loop conformation and comparative analysis with other endolysins. Proteins https://doi.org/10.1002/prot.25428
Pothineni, N.V.K., Karathanasis, S. K., Ding, Z., Arulandu, A., Varughese, K.I., Mehta, J.L. 2017. LOX-1 in Atherosclerosis and Myocardial Ischemia. Journal of the American College of Cardiology. https://doi.org/10.1016/j.jacc.2017.04.010
Krishna Das, B., Kumar, A., Maindola, P., Mahanty, S., Jain, S.K., Reddy, M.K., Arockiasamy, A. 2016. Non-native ligands define the active site of Pennisetum glaucum (L.) R. Br dehydroascorbate reductase. Biochemical and Biophysical Research Communications, 473. https://doi.org/10.1016/j.bbrc.2016.04.031
Verma, A., Chandele, A., Nayak, K., Kaja, M.K., Arulandu, A., Lodha, R., Ray, P. 2016. High yield expression and purification of Chikungunya virus E2 recombinant protein and its evaluation for serodiagnosis. Journal of Virological Methods, 235(July), 73–79. https://doi.org/10.1016/j.jviromet.2016.05.003
Maindola, P., Raina, R., Goyal, P., Atmakuri, K., Ojha, A., Gupta, S., Arockiasamy, A. 2014. Multiple enzymatic activities of ParB/Srx superfamily mediate sexual conflict among conjugative plasmids. Nature Communications, 5. https://doi.org/10.1038/ncomms6322
Balasubramaniam, D., Arockiasamy, A., Kumar, P.D., Sharma, A., Krishnaswamy, S. 2012. Asymmetric pore occupancy in crystal structure of OmpF porin from Salmonella typhi. Journal of Structural Biology, 178(3). https://doi.org/10.1016/j.jsb.2012.04.005
Kashyap, M., Jagga, Z., Das, B.K., Arockiasamy, A., Bhavesh, N.S. 2012. 1H, 13C and 15N NMR assignments of inactive form of P1 endolysin Lyz. Biomolecular NMR Assignments, 6(1). https://doi.org/10.1007/s12104-011-9331-4
Arockiasamy, A., Aggarwal, A., Savva, C.G., Holzenburg, A., Sacchettini, J.C. 2011. Crystal structure of calcium dodecin (Rv0379), from mycobacterium tuberculosis with a unique calcium-binding site. Protein Science, 20(5). https://doi.org/10.1002/pro.607
Sun, Q., Kuty, G. F., Arockiasamy, A., Xu, M., Young, R., Sacchettini, J. C. 2009. Regulation of a muralytic enzyme by dynamic membrane topology. Nature Structural and Molecular Biology, 16(11). https://doi.org/10.1038/nsmb.1681
Xu, M., Arulandu, A., Struck, D.K., Swanson, S., Sacchettini, J.C., Young, R. 2005. Disulfide isomerization after membrane release of its SAR domain activates P1 lysozyme. Science, 307(5706), 113–117. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/15637279
Arockiasamy, A., Murthy, G.S., Rukmini, M.R., Sundara Baalaji, N., Katpally, U.C., Krishnaswamy, S. 2004. Conformational epitope mapping of OmpC, a major cell surface antigen from Salmonella typhi. Journal of Structural Biology, 148(1). https://doi.org/10.1016/j.jsb.2004.03.011
Arockiasamy, A..Kumar, P.D., Sundara Baalaji, N., Rukmini, M.R., Krishnaswamy, S. 2004. Folding and structural stability of OmpC from Salmonella typhi: Role of LPS and environment. Curr. Sci. 87, 197-202
Sharma, V., Arockiasamy, A., Ronning, D.R., Savva, C.G., Holzenburg, A., Braunstein, M., Sacchettini, J.C. 2003. Crystal structure of Mycobacterium tuberculosis SecA, a preprotein translocating ATPase. Proceedings of the National Academy of Sciences of the United States of America, 100(5). https://doi.org/10.1073/pnas.0538077100
Sujatha, S., Arockiasamy, A., Krishnaswamy, S.,Usha, R. 2001. Molecular modelling of epitope presentation using membrane protein OmpC. Indian J. Biochem. Biophys. 38, 294-297
Arockiasamy, S., Krishnaswamy, S. 2000. Homology model of major surface antigen OmpC from Salmonella typhi and its functional implications. J. Biomol. Struct. Dyn. 18, 261-271
Arockiasamy, A., Krishnaswamy, S. 2000. Purification of integral outermembrane protein OmpC, a surface antigen from Salmonella typhi for structure-function studies; a method applicable to Enterobacterial major outer membrane protein. Anal. Biochem. 283, 64-70
Gnanasekaran, T.V., Suraj Peri, A. Arockiasamy, A.,Krishnaswamy, S. 2000. Profiles from structure based sequence alignment of porins can identify b-stranded membrane proteins. Bioinformatics 16, 839-842
Arockiasamy, A., Krishnaswamy, S. 1999. Crystallization of the immunodominant outer membrane protein OmpC; the first protein crystals from Salmonella typhi, a human pathogen. FEBS Letters, 453(3). https://doi.org/10.1016/S0014-5793(99)00746-2
Arockiasamy, A., Krishnaswamy, S. 1995. Prediction of B-cell epitopes for Salmonella typhi OmpC. Journal of Biosciences, 20(2). https://doi.org/10.1007/BF02703271