25-Hydroxyvitamin D₃

Vitamin D₃ carbon nomenclature
Summary of P450-dependent vitamin D₃ metabolism

Trivial names: 25-Hydroxyvitamin D₃; 25-Hydroxycholecalciferol; Calcidiol
Systematic name: (5Z,7E)-(3S)-9,10-Secocholesta-5,7,10(19)-triene-3,25-diol
Molecular formula: C₂₇H₄₄O₂

Substrate [1]

1,25-Dihydroxyvitamin D₃

steroid -hydroxylation at C1

1

CP27_HUMAN
CP27_RABIT
CP27_RAT

Substrate [2]

CP24_HUMAN
CP24_MOUSE
CP24_RAT

Product [3]

CP27_HUMAN
CP27_RABIT
CP27_RAT

References

1. Axén, E., Postlind, H., Sjöberg, H. and Wikvall, K. (1994) Liver mitochondrial cytochrome P450 CYP27 and recombinant-expressed human CYP27 catalyze 1-hydroxylation of 25-hydroxyvitamin D₃. Proc. Natl. Acad. Sci. USA 91, 10014-10018.
2. Dilworth, F.J., Scott, I., Green, A., Strugnell, S., Guo, Y.-D., Roberts, E.A., Kremer, R., Calverley, M.J., Makin, H.L.J. and Jones, G. (1995) Different mechanisms of hydroxylation site selection by liver and kidney cytochrome P450 species (CYP27 and CYP24) involved in vitamin D metabolism. J. Biol. Chem. 270, 16766-16774.
3. Usui, E., Noshiro, M., Ohyama, Y. and Okuda, K. (1990) Unique property of liver mitochondrial P450 to catalyze the two physiologically important reactions involved in both cholesterol catabolism and vitamin D activation. FEBS Lett. 274, 175-177.

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