May 1, 1995

Summary of P450s by family and subfamily

There are 14 mammalian families, but the CYP2, CYP4 and the CYP51 families
also contain non-mammalian members.

These are the mammalian families:

1, 2, 3, 4, 5, 7, 8, 11, 17, 19, 21, 24, 27, 51

Only five of these families have multiple subfamilies, they are:

CYP1 with two subfamilies
CYP2 with 14 subfamilies (six of these are non-mammalian: 2H = chicken,
        2K, 2M, 2N and 2P are fish, 2L = lobster, 2Q = Xenopus
CYP4 with 14 subfamilies (11 of these are from insects)
CYP7 with two subfamilies
CYP11 with two subfamilies

From this group of 14 families there are 44 subfamilies
and 26 of these are mammalian

Invertebrate families include 2, 4, 6, 9, 10, 12, 13, 14, 15 and 18
The only invertebrate in CYP2 is 2L1 from lobster (unpublished)
CYP4 has many insect subfamilies
CYP6 and CYP9 are exclusively insect families
CYP10 is from snail and it is probably a mitochondrial P450
CYP12 is a new insect family that clusters with mitochondrial P450s
CYP13 and 14 are from C. elegans and constitute rather large clusters of genes
CYP15 is a new insect P450
CYP18 is a new Drosophila sequence.

Lower eukaryotes occupy the numbers from CYP51-CYP62
CYP51 is also present in mammals and plants
CYP55 is from Fusaruim oxysporum but it is actually a bacterial
sequence closely related to the CYP105 family, so there are actually
11 lower eukaryote families.CYP52 has 5 subfamilies, CYP60 has
two subfamilies, while the rest do not have multiple subfamilies.

Plants are numbered CYP71 to CYP90
The CYP71, CYP76 and CYP81 families have multiple subfamilies

Bacteria have 18 families plus the CYP55 sequence = 19 families
There are a total of 30 subfamilies in bacteria if CYP55 is counted.
CYP102, CYP110 and probably CYP118 are eukaryote like and cluster
with the eukaryote sequences, not with the cam-like bacterial sequences.
CYP118 is from Mycobacterium leprae, but the sequence is poor and
needs to be repeated.

Total number of families is 70
Total number of subfamilies is 127
Total number of sequences is 477 in the sequence alignment
Some of these are short ESTs or PCR fragments

David R. Nelson