Archive of Kirill's P450 Café

Archive of Kirill's P450 Café - 2002

Last modified: Fri Dec 5 16:37:07 GMT 2003

20 December 2002.

Mifsud, W. and Bateman, A. (2002) Membrane-bound progesterone receptors contain a cytochrome b₅-like ligand-binding domain. Genome Biol. 3, 0068.

13 December 2002.

Porter, T.D. (2002) The roles of cytochrome b₅ in cytochrome P450 reactions. J. Biochem. Mol. Toxicol. 16, 311-316.

6 December 2002.

Mouse Genome Sequencing Consortium (2002) Initial sequencing and comparative analysis of the mouse genome. Nature 420, 520-562.

CYP families in mouse, human and pufferfish

29 November 2002.

Oesch-Bartlomowicz, B. and Oesch, F. (2002) Fast regulation of cytochrome P450 activities by phosphorylation and consequences for drug metabolism and toxicity. Biol. Chem. 383, 1587-1592.

22 November 2002.

Leys, D., Mowat, C.G., McLean, K.J., Richmond, A., Chapman, S.K., Walkinshaw, M.D. and Munro, A.W. (2003) Atomic structure of Mycobacterium tuberculosis CYP121 to 1.06 Å reveals novel features of cytochrome P450. J. Biol. Chem. 278, 5141-5147.

15 November 2002.

Tijet, N. and Brash, A.R. (2002) Allene oxide synthases and allene oxides. Prostaglandins Other Lipid Mediat. 68-69, 423-431.

8 November 2002.

De Mot, R. and Parret, A.H.A. (2002) A novel class of self-sufficient cytochrome P450 monooxygenases in prokaryotes. Trends Microbiol. 10, 502-508.

1 November 2002.

Yano, J.K., Blasco, F., Li, H., Schmid, R.D., Henne, A. and Poulos, T.L. (2003) Preliminary characterization and crystal structure of a thermostable cytochrome P450 from Thermus thermophilus. J. Biol. Chem. 278, 608-616.

25 October 2002.

Glieder, A., Farinas, E.T. and Arnold, F.H. (2002) Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase. Nature Biotechnol. 20, 1135-1139.

18 October 2002.

Elmore, C.L. and Porter, T.D. (2002) Modification of the nucleotide cofactor-binding site of cytochrome P450 reductase to enhance turnover with NADH in vivo. J. Biol. Chem. 277, 48960-48964.

11 October 2002.

Knight, K. and Scrutton, N.S. (2002) Stopped-flow kinetic studies of electron transfer in the reductase domain of neuronal nitric oxide synthase: re-evaluation of the kinetic mechanism reveals new enzyme intermediates and variation with cytochrome P450 reductase. Biochem. J. 367, 19-30

4 October 2002.

Dabrowski, M.J., Schrag, M.L., Wienkers, L.C. and Atkins, W.M. (2002) Pyrene·pyrene complexes at the active site of cytochrome P450 3A4: evidence for a multiple substrate binding site. J. Am. Chem. Soc. 124, 11866-11867

27 September 2002.

de Visser, S.P., Ogliaro, F., Sharma, P.K. and Shaik, S. (2002) What factors affect the regioselectivity of oxidation by cytochrome P450? A DFT study of allylic hydroxylation and double bond epoxidation in a model reaction. J. Am. Chem. Soc. 124, 11809-11826 [Supplementary Material]

20 September 2002.

Deprez, E., Gill, E., Helms, V., Wade, R. and Hui Bon Hoa, G. (2002) Specific and non-specific effects of potassium cations on substrate-protein interactions in cytochromes P450cam and P450lin. J. Inorg. Biochem. 91, 597-606.

This review appears in the special issue on Advances in the Inorganic Biochemistry of Cytochrome P450 (Journal of Inorganic Biochemistry vol. 91, no. 4, 2002). This issue contains selected papers presented on 12th International Conference on Cytochrome P450, 11-15 September 2001, La Grande Motte, France.

13 September 2002.

Zhang, L., Kudo, T., Takaya, N. and Shoun, H. (2002) The B' helix determines cytochrome P450nor specificity for the electron donors NADH and NADPH. J. Biol. Chem. 37, 33842-33847.

6 September 2002.

Hahn, M.E. (2002) Aryl hydrocarbon receptors: diversity and evolution. Chem. Biol. Interact. 141, 131-160.

This review appears in the Special Issue on AhR biology (Chemico-Biological Interactions vol. 141, no. 1-2, 2002).

30 August 2002.

Dunn, A.R., Hays, A.-M.A., Goodin, D.B., Stout, C.D., Chiu, R., Winkler, J.R. and Gray, H. (2002) Fluorescent probes for cytochrome P450 structural characterization and inhibitor screening. J. Am. Chem. Soc. 124, 10254-10255. [Supplementary Material]

23 August 2002.

Grogan, G., Roberts, G.A., Parsons, S., Turner, N.J. and Flitsch, S.L. (2002) P450_camr, a cytochrome P450 catalysing the stereospecific 6-endo-hydroxylation of (1R)-(+)-camphor. Appl. Microbiol. Biotechnol. 59, 449-454.

16 August 2002.

Taoka, S., Lepore, B.W., Kabil, Ö., Ojha, S., Ringe, D. and Banerjee, R. (2002) Human cystathionine ß-synthase is a heme sensor protein. Evidence that the redox sensor is heme and not the vicinal cysteines in the CXXC motif seen in the crystal structure of the truncated enzyme. Biochemistry 41, 10454-10461.

9 August 2002.

Suzuki, N., Higuchi, T. and Nagano, T. (2002) Multiple active intermediates in oxidation reaction catalyzed by synthetic heme-thiolate complex relevant to cytochrome P450. J. Am. Chem. Soc. 124, 9622-9628. [Supplementary Material]

Structure of the synthetic heme-thiolate complex

2 August 2002.

Gan, J.-H., Wu, J., Wang, Z.-Q., Wang, Y.-H., Huang, Z.-X. and Xia, Z.-X. (2002) Structures of V45E and V45Y mutants and structure comparison of a variety of cytochrome b₅ mutants. Acta Crystallogr. D58, 1298-1306.

The heme and ligands of V45Y and wild-type cytb5

26 July 2002.

Kim, J.-S., Ahn, T., Yim, S.-K. and Yun, C.-H. (2002) Differential effect of copper (II) on the cytochrome P450 enzymes and NADPH-cytochrome P450 reductase: inhibition of cytochrome P450-catalyzed reactions by copper (II) ion. Biochemistry 41, 9438-9447.

19 July 2002.

Roberts, G.A., Grogan, G., Greter, A., Flitsch, S.L. and Turner, N.J. (2002) Identification of a new class of cytochrome P450 from a Rhodococcus sp. J. Bacteriol. 184, 3898-3908.

At the C-terminus, the P450 is fused to a reductase domain which displays sequence conservation for dioxygenase reductase proteins.

12 July 2002.

Fradera, X., de la Cruz, X., Silva, C.H.T.P., Gelpí, J.L., Luque, F.J. and Orozco, M. (2002) Ligand-induced changes in the binding sites of proteins. Bioinformatics 18, 939-948.

5 July 2002.

Takaya, N., Uchimura, H., Lai, Y. and Shoun, H. (2002) Transcriptional control of nitric oxide reductase gene (CYP55) in the fungal denitrifier Fusarium oxysporum. Biosci. Biotechnol. Biochem. 66, 1039-1045.

21 June 2002.

Beilke, D., Weiss, R., Löhr, F., Pristovsek, P., Hannemann, F., Bernhardt, R. and Rüterjans, H. (2002) A new electron transport mechanism in mitochondrial steroid hydroxylase systems based on structural changes upon the reduction of adrenodoxin. Biochemistry 41, 7969-7978.

14 June 2002.

Yu, B., Ruppert, M. and Stöckigt, J. (2002) Deoxysarpagine hydroxylase - a novel enzyme closing a short side pathway of alkaloid biosynthesis in Rauvolfia. Bioorg. Med. Chem. 10, 2479-2483.

31 May 2002.

Dixon, R.A. and Ferreira, D. (2002) Genistein. Phytochemistry 60, 205-211.

24 May 2002.

Lamb, D.C., Fowler, K., Kieser, T., Manning, N., Podust, L.M., Waterman, M.R., Kelly, D.E. and Kelly, S.L. (2002) Sterol 14-demethylase activity in Streptomyces coelicolor A3(2) is associated with an unusual member of the CYP51 gene family. Biochem. J. 364, 555-562.

17 May 2002.

Guengerich, F.P. (2002) Cytochrome P450 enzymes in the generation of commercial products. Nature Rev. Drug Discovery 1, 359-366.

10 May 2002.

Bayburt, T.H. and Sligar, S.G. (2002) Single-molecule height measurements on microsomal cytochrome P450 in nanometer-scale phospholipid bilayer disks. Proc. Natl. Acad. Sci. USA 99, 6725-6730.

Two models of P450 interacting with membrane

3 May 2002.

Hui Bon Hoa, G., McLean, M.A. and Sligar, S.G. (2002) High pressure, a tool for exploring heme protein active sites. Biochim. Biophys. Acta 1595, 297-308.

26 April 2002.

Dawson, J.H., Pond, A.E. and Roach, M.P. (2002) H93G myoglobin cavity mutant as versatile template for modeling heme proteins: Magnetic circular dichroism studies of thiolate- and imidazole-ligated complexes. Biopolymers 67, 200-206.
This review appears in the Special Issue: Selections from the 9th European Conference on the Spectroscopy of Biological Materials (Biopolymers vol. 67, no. 4-5, 2002).

19 April 2002.

Guallar, V., Gherman, B.F., Lippard, S.J. and Friesner, R.A. (2002) Quantum chemical studies of methane monooxygenase: comparision with P450. Curr. Opin. Chem. Biol. 6, 236-242.
This review appears in the Guest section on Computational bioinorganic chemistry (Part II) (Current Opinion in Chemical Biology vol. 6, no. 2, 2002).

12 April 2002.

Mowat, C.G., Leys, D., McLean, K.J., Rivers, S.L., Richmond, A., Munro, A.W., Ortiz Lombardia, M., Alzari, P.M., Reid, G.A., Chapman, S.K. and Walkinshaw, M.D. (2002) Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis. Acta Crystallogr. D58, 704-705.

P450 MT2 (CYP121) has been crystallised. The native crystal diffracts to 1.6 Å resolution and Hg-derivative crystal data were collected to a resolution of 2.5 Å.

5 April 2002.

Yao, P., Wang, Y.-H., Sun, B.-Y., Xie, Y., Hirota, S., Yamauchi, O. and Huang, Z.-X. (2002) Kinetic studies on the oxidation of cytochrome b₅ Phe35 mutants with cytochrome c, plastocyanin and inorganic complexes. J. Biol. Inorg. Chem. 7, 375-383.

29 March 2002.

Breslow R. (2001) Biomimetic selectivity. Chem. Rec. 1, 3-11.

22 March 2002.

Raucy, J.L. and Allen, S.W. (2001) Recent advances in P450 research. Pharmacogenomics J. 1, 178-186.

15 March 2002.

Champier, L., Sibille, N., Bersch, B., Brutscher, B., Blackledge, M. and Covès, J. (2002) Reactivity, secondary structure, and molecular topology of the Escherichia coli sulfite reductase flavodoxin-like domain. Biochemistry 41, 3770-3780.

8 March 2002.

Orellana, M., Varela, N., Guajardo, V., Araya, J. and Rodrigo R. (2002) Modulation of rat liver cytochrome P450 activity by prolonged red wine consumption. Comp. Biochem. Physiol. C 131, 161-166.

1 March 2002.

Gilardi, G., Meharenna, Y.T., Tsotsou, G.E., Sadeghi, S.J., Fairhead, M. and Giannini, S. (2002) Molecular Lego: design of molecular assemblies of P450 enzymes for nanobiotechnology. Biosensors and Bioelectronics 17, 133-145.

22 February 2002.

Ekins, S. and Schuetz, E. (2002) The PXR crystal structure: the end of the beginning. Trends Pharmacol. Sci. 23, 49-50.

Related publication:

Watkins, R.E., Wisely, G.B., Moore, L.B., Collins, J.L., Lambert, M.H., Williams, S.P., Willson, T.M., Kliewer, S.A. and Redinbo, M.R. (2001) The human nuclear xenobiotic receptor PXR: structural determinants of directed promiscuity. Science 292, 2329-2333.

15 February 2002.

Ourlin, J.C., Handschin, C., Kaufmann, M. and Meyer, U.A. (2002) A link between cholesterol levels and phenobarbital induction of cytochromes P450. Biochem. Biophys. Res. Commun. 291, 378-384.

8 February 2002.

Matsunaga, I., Yamada, A., Lee, D.-S., Obayashi, E., Fujiwara, N., Kobayashi, K., Ogura, H. and Shiro, Y. (2002) Enzymatic reaction of hydrogen peroxide-dependent peroxygenase cytochrome P450s: kinetic deuterium isotope effects and analyses by resonance Raman spectroscopy. Biochemistry 41, 1886-1892.

substrate relative to the heme moiety of peroxygenases

1 February 2002.

LeBrun, L.A., Hoch, U. and Ortiz de Montellano, P.R. (2002) Autocatalytic mechanism and consequences of covalent heme attachment in the cytochrome P4504A family. J. Biol. Chem. 277, 12755-12761.

25 January 2002.

Boireau, W., Bombard, S., Sari, M.-A. and Pompon, D. (2002) Bioengineering and characterization of DNA-protein assemblies floating on supported membranes. Biotechnol. Bioeng. 77, 225-231.

A biodevice involving thiolated ssDNA and engineered cytochrome b₅ linked through a cisplatin bridge is described.

18 January 2002.

Cavalli, A. and Recanatini, M. (2002) Looking for selectivity among cytochrome P450s inhibitors. J. Med. Chem. 45, 251-254.

11 January 2002.

Haralampidis, K., Trojanowska, M. and Osbourn, A.E. (2002) Biosynthesis of triterpenoid saponins in plants. Adv. Biochem. Eng. Biotechnol. 75, 31-49.

4 January 2002.

Lewis, D.F.V. and Dickins, M. (2002) Factors influencing rates and clearance in P450-mediated reactions: QSARs for substrates of the xenobiotic-metabolizing hepatic microsomal P450s. Toxicology 170, 45-53.

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Archive of Kirill's P450 Café - 2001
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