P450s that have appeared since the 1993 P450 nomenclature update.
Part C covering CYP10 to CYP69
Animal P450 names continue at CYP301 in this file
Lower eukaryote names continue at CYP501 in this file
ALL Drosophila melanogaster P450S ARE NOW INCLUDED
This includes list references that were incomplete and duplications of
sequences that were already in the update. If a sequence is assigned an
accession number that was not in the old update it is included in this list.
Some expressed sequence tags (ESTs) are also included from humans.
This list was last revised on Feb. 4, 2003.
Added all Neurospora P450s see 527A1-553A1
Added CYP51 Trypanosoma brucei
Added Fugu P450s, added CYP505A3 added CYP18 Spodoptera
Added all human genes and pseudogenes
Added 104 Anopheles genes
Revised May 13, 2003 named all remaining Dictyostelium P450 genes
Compiled by David R. Nelson
There are 80 C. elegans P450s listed here, surpassing the known mouse and human
complements. The C. elegans genome is now about 90% finished sequence and 9%
unfinished (one strand or one chemistry). About 1Mb is not cloned at three
telomeres and two internal sites. A special issue of Science appeared in Dec. 98 even though the genome is not completely done. The amount of sequence in the Blast searchable database at Washington Univ. is 140Mb, more than the 100Mb size of the genome. Therefore, we can guess that this set includes all the P450 genes in C. elegans, but the distrubution is not even. Most P450 genes (43 genes) are on chromosome V. see additional info on C. elegans P450s see this list. To see the actual sequences go to the C. elegans sequence file. So far we are missing CYP11A, CYP11B, CYP17, CYP19, CYP21, CYP24 and CYP27A and CYP27B. Does C. elegans make steroids? The present evidence would suggest not. Does C. elegans have
mitochondrial P450s? There is one probable mitochondrial P450 in C. elegans on
cosmid ZK177 named CYP44. It was thought to be incomplete but now a full length sequence
has been assembled from the genomic sequence.
10A Subfamily
11A Subfamily
11B Subfamily
12A Subfamily
12B Subfamily
13A Subfamily
13B Subfamily
14A Subfamily
16A Subfamily
17A Subfamily
18A Subfamily
19A Subfamily
21A Subfamily
22A Subfamily
23A Subfamily
24A Subfamily
25A Subfamily
26A Subfamily
27A Subfamily
27B Subfamily
28A Subfamily
29A Subfamily
30A Subfamily
31A Subfamily
32A Subfamily
33A Subfamily
33B Subfamily
33C Subfamily
33D Subfamily
33E Subfamily
34A Subfamily
35A Subfamily
35B Subfamily
35C Subfamily
35D Subfamily
36A Subfamily
37A Subfamily
37B Subfamily
40A Subfamily
41A Subfamily
42A Subfamily
43A Subfamily
44A Subfamily
45A Subfamily
46A Subfamily
51A Subfamily
52A Subfamily
52B Subfamily
52C Subfamily
52D Subfamily
52E Subfamily
52F Subfamily
53A Subfamily
53B Subfamily
54A Subfamily
55A Subfamily
56A Subfamily
57A Subfamily
58A Subfamily
59A Subfamily
60A Subfamily
60B Subfamily
61A Subfamily
62A Subfamily
63A Subfamily
64A Subfamily
65A Subfamily
66A Subfamily
67A Subfamily
10A Subfamily
CYP10 Lymnaea stagnalis (pond snail)
GenEMBL S46130 (1870bp) PIR JX0225 (545 amino acids)
Teunissen,Y., Geraerts,W.P., van Heerikhuizen,H., Planta,R.J.
and Joosse,J.
Molecular cloning of a cDNA encoding a member of a novel
cytochrome P450 family in the mollusc Lymnaea stagnalis.
J. Biochem. 112, 249-252 (1992)
11A Subfamily
CYP11A1 human
PIR A48733 (239 amino acids)
Matteson, K.J., Chung, B.C., Urdea, M.S. and Miller, W.L.
Study of cholesterol side-chain cleavage (20,22 desmolase)
deficiency causing congenital lipoid adrenal hyperplasia
using bovine-sequence P450scc oligodeoxyribonucleotide
probes.
Endocrinology 118, 1296-1305 (1986)
CYP11A1 rabbit
GenEMBL S59219 (1336bp) PIR A49189 (445 amino acids)
Yang,X., Iwamoto,K., Wang,M., Artwhol,J., Mason,J.I. and
Pang,S.
Inherited congenital adrenal hyperplasia in the rabbit is caused by
a deletion in the gene encoding cytochrome P450 cholesterol side
chain cleavage enzyme.
Endocrinol. 132, 1977-1982 (1993)
CYP11A1 bovine
PIR A42033 (18 amino acids)
Pikuleva, I.A., Lapko, A.G., Chashchin, V.L.
Functional reconstitution of cytochrome P-450-scc with hemin
activated with Woodward's reagent K. Formation of a
hemeprotein cross-link.
J. Biol. Chem. 267, 1438-1442 (1992)
CYP11A1 Sus scrofa (pig)
GenEMBL L34259 (2376bp)
Urban,R.J., Shupnik,M.A. and Bodenburg,Y.H.
Insulin-like growth factor-I increases expression of the porcine
P-450 cholesterol side chain cleavage gene through a GC-rich domain.
J. Biol. Chem. 269, 25761-25769 (1994)
CYP11A1 goat
GenEMBL D50058 (1825bp)
Okuyama,E., Okazaki,T., Furukawa,A., Wu,R.-F. and Ichikawa,Y.
Molecular cloning and nucleotide sequences of cDNA clones of sheep
and goat
adrenolcortical cytochrome P450scc.
unpublished (1995)
CYP11A1 sheep
GenEMBL D50057 (1825bp)
Okuyama,E., Okazaki,T., Furukawa,A., Wu,R.-F. and Ichikawa,Y.
Molecular cloning and nucleotide sequences of cDNA clones of sheep
and goat
adrenolcortical cytochrome P450scc.
unpublished (1995)
CYP11A1 bovine
PIR S29644 (21 amino acids)
Tsujita, M. and Ichikawa, Y.
Substrate-binding region of cytochrome P-450(scc) (P-450
XIA1). Identification and primary structure of the
cholesterol binding region in cytochrome P-450(scc).
Biochim. Biophys. Acta 1161, 124-130 (1993)
Cyp11a1 mouse
GenEMBL J05511
Rice,D.A., Kirkman,M.S., Aitkin,L.D., Mouw,A.R., Schimmer,B.P. and
Parker,K.L.
Analysis of the promoter region of the gene encoding mouse
cholesterol side-chain cleavage enzyme
J. Biol. Chem. 265, 11713-11720 (1990)
CYP11A1 Oncorhynchus mykiss (rainbow trout)
GenEMBL S57305 (1789bp) Swiss Q07217 (514 amino acids)
PIR S32197 (514 amino acids)
Takahashi,M., Tanaka,M., Sakai,N., Adachi,S., Miller,W.L.
and Nagahama,Y.
Rainbow trout ovarian cholesterol side-chain cleavage
cytochrome P450 (P450scc). cDNA cloning and mRNA expression
during oogenesis.
FEBS Lett. 319, 45-48 (1993)
CYP11A1 Fugu rubripes (pufferfish)
No accession number
Scaffold_1630
37515 MARWSVWRSPVVLPLSRMEVPMTGARHSSTMPVARQTYSDSSSFV 37381
37380 RSFNDIPGLWKNGVANLYNFWKLDGFRNLHHIMVQNFNTFGPIYR 37246 (2)
37065 EKIGYYESVNIINPEDAAILFKAEGHYPKRLKVEAWTSYRDYRNRKYGVLLK 36904 (2)
36822 NGEEWRCNRVLLNKEVISPKVLENFVPLLDEVGNDFVVRVHKKIARSGQNKWTTDLSQELFKYALES 36628 (1)
36506 VSSVLYGERLGLFLDYIDPEAQHFIDCISLMFKTTSPML
YIPPALLRKVGAKVWRDHVEAWDGIFNQ 36300 (1 expected) bad boundary
36208 ADRCIQNIYRRLRQETGPSKKYPGVLASLLLRDKLSIEDIKASITELMAGGVDT 36047 (0)
35975 TSITLLWTLYELARHPNLQEELRAEVAAARTESQGDMLEMLKRIPLVKGALKETLR 35808 (2)
35731 LHPVAVSLQRYIAEDIIIQNYHIPAG 35654 (0)
35568 TLVQLGLYAMGRDPKVFFRPEQYQPSRWLRSETHYFKSLGFGFGPRQCLGRRIAEAEMQLFLIH 35377 (0)
35297 MLENFRVEKQRHMEVQSTFELILLPDKPIILTLKPLSS* 35181
CYP11A1 Dasyatis americana (southern stingray)
GenEMBL U63299(4619bp)
Nunez,B.S. and Trant,J.M.
Isolation of the cDNA encoding the interrenal form of cholesterol
side chain cleavage cytochrome P450 of the southern stingray
(Dasyatis americana).
unpublished (1996)
11B Subfamily
CYP11B1 human
GenEMBL J05140 M32863 (1482bp) M32878 (2633bp) M32879 (1155bp)
Mornet,E., Dupont,J., Vitek,A. and White,P.C.
Characterization of two genes encoding human steroid 11-beta-
hydroxylase (P-450-11-beta).
J. Biol. Chem. 264, 20961-20967 (1989)
CYP11B1 human
GenEMBL D16155 (156bp)
Naiki,Y., Shizuta,Y., Kawamoto,T., Yasushiro,M., Miyahara,K.,
Toda,K., Tadao,O. and Imura,H.
A nonsense mutation (TGG(116Arg)-TAG(stop)) in CYP11B1
causes steroid 11beta-hydroxylase deficiency.
J. Clin. Endocrinol. Metab. 77, 1677-1682 (1993)
CYP11B1 human
GenEMBL D10169 D90428 (2085bp)
Kawamoto,T., Mitsuuchi,Y., Toda,K., Yokoyama,Y., Miyahara,K.,
Miura,S., Ohnishi,T., Ichikawa,Y., Nakao,K., Imura,H., Ulick,S.
and Shizuta,Y.
Role of steroid 11beta-hydroxylase and steroid 18-hydroxylase in the
biosynthesis of glucocorticoids and mineralocorticoids in humans.
Proc. Natl. Acad. Sci. USA 89, 1458-1462 (1992)
CYP11B1 human
PIR S29068 (30 amino acids)
Kawamoto, T., Mitsuuchi, Y., Toda, K., Miyahara, K.,
Yokoyama, Y., Nakao, K., Hosoda, K., Yamamoto, Y., Imura,
H. and Shizuta, Y.
Cloning of cDNA and genomic DNA for human cytochrome P-450
(11-beta).
FEBS Lett. 269, 345-349 (1990)
CYP11B1 Papio hamadryas ursinus (chacma baboon)
GenEMBL U52085(228bp)
Hampf,M., Swart,A. and Swart,P.
Expression of Papio ursinus steroid 11beta hydroxylase
unpublished (1996)
CYP11B1 rat
GenEMBL D10107 (1528bp) S58847
PIR B46040 (499 amino acids)
Matsukawa,N., Nonaka,Y., Higaki,J., Nagano,M., Mikami,H.,
Ogihara,T. and Okamoto,M.
Dahl's salt-resistant normotensive rat has mutations in
cytochrome P450 (11 beta), but the salt-sensitive hypertensive
rat does not.
J. Biol. Chem. 268, 9117-9121 (1993)
Note: only 1 amino acid difference with 11B1 at position 84
E (normal) changed to G (seen in 11B2 and 11B3)
D10107 has five differences with D11354
CYP11B1 rat
GenEMBL S58858 (330bp) PIR JX0251(499 amino acids)
Nomura,M., Morohashi, K.-i., Kirita, S., Nonaka, Y.,
Okamoto,M., Nawata,H. and Omura,T.
Three forms of rat CYP11B genes: 11 beta-hydroxylase gene,
aldosterone synthase gene, and a novel gene.
J. Biochem. 113, 144-152 (1993)
Note: Fig. 3 has 6 errors. 1,2) aldo-46 amino acids 2 and 3 are
incorrectly translated gctctc = AL not HS. 3) 11B3 codon at 559-561
incorrectly translated gac = D not N. 4,5,6) 11beta-62, 11B1
and 11B3 codon at 964-966 tcc = Ser not Pro.
CYP11B1 rat
GenEMBL S58849, D14086 to D14091 PIR A46039 (499 amino acids)
Mukai,K., Imai,M., Shimada,H. and Ishimura,Y.
Isolation and characterization of rat CYP11B genes involved
in late steps of mineralo- and glucocorticoid syntheses.
J. Biol. Chem. 268, 9130-9137 (1993)
CYP11B1 rat
GenEMBL S63899 (596bp)
Mukai,K., Imai,M., Shimada,H., Okada,Y., Ogishima,T. and
Ishimura,Y.
Structural differences in 5'-flanking regions of rat cytochrome
P-450aldo and P-450(11) beta genes
Biochem. Biophys. Res. Commun. 180, 1187-1193 (1991)
CYP11B1 rat
GenEMBL D11354 (1528bp) PIR A46040 (499 amino acids)
Matsukawa,N., Nonaka,Y., Higaki,J., Nagano,M., Mikami,H.,
Ogihara,T. and Okamoto,M.
Dahl's salt-resistant normotensive rat has mutations in
cytochrome P450 (11 beta), but the salt-sensitive hypertensive
rat does not.
J. Biol. Chem. 268, 9117-9121 (1993)
Note: This sequence is the wild type DS rat sequence. The mutant
DR rat has five amino acids differences accession number D10107
CYP11B1 Cavia porcellus (guinea pig)
GenEMBL Z69785(2028bp)
Bulow,H.E., Mobius,K., Bahr,V. and Bernhardt,R.
Molecular cloning and functional expression of the cytochrome P450
11B-hydroxylase of the guinea pig.
Biochem. Biophys. Res. Commun. 221, 304-312 (1996)
CYP11B1 bovine
PIR JX0151 (503 amino acids)
Kirita, S., Hashimoto, T., Kitajima, M., Honda, S.,
Morohashi, K. and Omura, T.
Structural analysis of multiple bovine P-450(11beta) genes
and their promoter activities.
J. Biochem. 108, 1030-1041 (1990)
CYP11B1 pig
GenEMBL D38590(1671bp)
Sun,T., Zhao,Y., Nonaka,Y. and Okamoto,M.
Cloning and expression of cytochrome P450(11 beta) of porcine
adrenal cortex.
J. Steroid Biochem. Mol. Biol. 52, 227-232 (1995)
CYP11B1 sheep
GenEMBL L34337 (1639bp)
Boon,W.C., Roche,P.J., Hammond,V.E., Jeyaseelan,K.,
Crawford,R.J. and Coghlan,J.P.
Cloning and expression analysis of a cytochrome P450-11-beta
cDNA in sheep.
unpublished (1994)
CYP11B1 Ovis aries (sheep)
GenEMBL L28716 (2300bp)
Anwar,A., Jeyaseelan,K. and Coghlan,J.P.
Molecular cloning and characterization of the ovine CYP11B1
promoter.
Biochem. Mol. Biol. Int. 33, 1169-1178 (1994)
CYP11B1 Ovis ammon (sheep)
GenEMBL L47569(9027bp)
Anwar,A., Jeyaseelan,K. and Coghlan,J.P.
Characterization of an ovine 11-beta hydroxylase (cyp11b) gene.
unpublished (1995)
Cyp11b1 mouse
PIR A41552 (500 amino acids)
Domalik, L.J., Chaplin, D.D., Kirkman, M.S., Wu, R.C., Liu,
W., Howard, T.A., Seldin, M.F., Parker, K.L.
Different isozymes of mouse 11beta-hydroxylase produce
mineralocorticoids and glucocorticoids.
Mol. Endocrinol. 5, 1853-1861 (1991)
Cyp11b1 mouse
PIR A32210 (42 amino acids)
Mouw, A.R., Rice, D.A., Meade, J.C., Chua, S.C., White, P.C.,
Schimmer, B.P. and Parker, K.L.
Structural and functional analysis of the promoter region of
the gene encoding mouse steroid 11beta-hydroxylase.
J. Biol. Chem. 264, 1305-1309 (1989)
CYP11B2 human
GenEMBL J05140 M32864 (1809bp) M32880 (3088bp) M32881 (1101bp)
Mornet,E., Dupont,J., Vitek,A. and White,P.C.
Characterization of two genes encoding human steroid 11-beta-
hydroxylase (P-450-11-beta).
J. Biol. Chem. 264, 20961-20967 (1989)
CYP11B2 human
GenEMBL D90429 D10170 (2114bp)
Kawamoto,T., Mitsuuchi,Y., Toda,K., Yokoyama,Y., Miyahara,K.,
Miura,S., Ohnishi,T., Ichikawa,Y., Nakao,K., Imura,H., Ulick,S.
and Shizuta,Y.
Role of steroid 11beta-hydroxylase and steroid 18-hydroxylase in the
biosynthesis of glucocorticoids and mineralocorticoids in humans.
Proc. Natl. Acad. Sci. USA 89, 1458-1462 (1992)
CYP11B2 human
GenEMBL D13752(6910bp)
Kawamoto,T., Mitsuuchi,Y., Ohnishi,T., Ichikawa,Y., Yokoyama,Y.,
Sumimoto,H., Toda,K., Miyahara,K., Kuribayashi,I., Nakao,K.,
Hosoda,K., Yamamoto,Y., Imura,H. and Shizuta,Y.
Cloning and expression of a cDNA for human cytochrome P-450aldo as
related to primary aldosteronism.
Biochem. Biophys. Res. Commun. 173 (1), 309-316 (1990)
CYP11B2 human
GenEMBL S77398 S77401 S77403 S77406 S77409 (genomic sequences)
Shizuta,Y., Kawamoto,T., Mitsuuchi,Y., Miyahara,K., Rosler,A.,
Ulick,S. and Imura,H.
Inborn errors of aldosterone biosynthesis in humans.
Steroids 60 (1), 15-21 (1995)
CYP11B2 rat
GenEMBL S58859 (353bp) PIR JX0252 (500 amino acids)
Nomura,M., Morohashi, K.-i., Kirita, S., Nonaka, Y.,
Okamoto,M., Nawata,H. and Omura,T.
Three forms of rat CYP11B genes: 11 beta-hydroxylase gene,
aldosterone synthase gene, and a novel gene.
J. Biochem. 113, 144-152 (1993)
CYP11B2 rat
GenEMBL S58850 D14092 to D14097
PIR B46039 (500 amino acids)
Mukai,K., Imai,M., Shimada,H. and Ishimura,Y.
Isolation and characterization of rat CYP11B genes involved
in late steps of mineralo- and glucocorticoid syntheses.
J. Biol. Chem. 268, 9130-9137 (1993)
CYP11B2 rat
GenEMBL S63898 (594bp)
Mukai,K., Imai,M., Shimada,H., Okada,Y., Ogishima,T. and Ishimura,Y.
Structural differences in 5'-flanking regions of rat cytochrome
P-450aldo and P-450(11) beta genes.
Biochem. Biophys. Res. Commun. 180, 1187-1193 (1991)
CYP11B2 rat
GenEMBL S64136 (3001bp) PIR JN0615 (506 amino acids)
GenEMBL U14908 (3000bp)
Zhou,M. and Gomez-Sanchez,C.E.
Cloning and expression of a rat cytochrome P-450 11
beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant.
Biochem. Biophys. Res. Commun. 194, 112-117 (1993)
Erratum: Biochem. Biophys. Res. Commun. 196, 1018 (1993)
CYP11B2 rat
PIR A34281 (20 amino acids) Swiss P30099 (510 amino acids)
Ogishima, T., Mitani, F., Ishimura, Y.
Isolation of aldosterone synthase cytochrome P-450 from zona
glomerulosa mitochondria of rat adrenal cortex.
J. Biol. Chem. 264, 10935-10938 (1989)
Note: sequence is from N-terminal after signal sequence
CYP11B2 rat
Swiss P30099 (510 amino acids) GenEMBL D00567 (2824bp)
Swiss P30100 (500 amino acids) GenEMBL D00568 (2705bp)
Matsukawa,N., Nonaka,Y., Ying,Z., Higaki,J., Ogihara,T. and
Okamoto,M.
Molecular cloning and expression of cDNAs encoding rat aldosterone
synthase: variants of cytochrome P-450 11beta
Biochem. Biophys. Res. Commun. 169, 245-252 (1990)
CYP11B2 hamster
GenEMBL S73810 (1503bp)
LeHoux,J.G., Mason,J.I., Bernard,H., Ducharme,L., LeHoux,J.,
Veronneau,S. and Lefebvre,A.
The presence of two cytochrome P450 aldosterone synthase mRNAs in
the hamster adrenal.
J. Steroid Biochem. Mol. Biol. 49, 131-137 (1994)
CYP11B2 cavia porcellus (domestic guinea pig)
GenEMBL AF018569
Buelow,H.E. and Bernhardt,R.
Molecular Cloning and Functional Expression of the Guinea Pig
Aldosterone Synthase.
Unpublished
CYP11B2 Rana catesbeiana (bullfrog)
GenEMBL D10984 (1919bp)
Nonaka,Y., Takemori,H., Halder,S.K., Sun,T., Ohta,M., Hatano,O.,
Takakusa,A. and Okamoto,M.
Frog Cytochrome P-450 (11 beta, aldo), a single enzyme involved in
the final steps
of glucocorticoid and mineralocorticoid biosynthesis.
Eur. J. Biochem. 229, 249-256 (1995)
Cyp11b2 mouse
GenEMBL S85260 (2804bp)
Domalik,L.J., Chaplin,D.D., Kirkman,M.S., Wu,R.C., Liu,W.W.,
Howard,T.A., Seldin,M.F. and Parker,K.L.
Different isozymes of mouse 11 beta-hydroxylase produce
mineralocorticoids and glucocorticoids.
Mol. Endocrinol. 5, 1853-1861 (1991)
CYP11B3 rat
PIR JX0253 (498 amino acids)
Nomura,M., Morohashi, K.-i., Kirita, S., Nonaka, Y.,
Okamoto,M., Nawata,H. and Omura,T.
Three forms of rat CYP11B genes: 11 beta-hydroxylase gene,
aldosterone synthase gene, and a novel gene.
J. Biochem. 113, 144-152 (1993)
CYP11B3 rat
GenEMBL U14907 (1497bp)
Zhou,M., Gomez-Sanchez,E.P., Foecking,M. and Gomez-Sanchez,C.E.
Cloning of the cytochrome P-450 CYP11B3 complementary DNA in the
rat.
unpublished
CYP11B3 rat
GenEMBL S59144 D14098 to D14103
Mukai,K., Imai,M., Shimada,H. and Ishimura,Y.
Isolation and characterization of rat CYP11B genes involved
in late steps of mineralo- and glucocorticoid syntheses.
J. Biol. Chem. 268, 9130-9137 (1993)
Note: only one amino acid difference with Nomura's 11B3
CYP11B3 rat
GenEMBL U17082(1497bp)
Mellon,S.H., Bair,S.R. and Monis,H.
P450c11B3 mRNA, transcribed from a third P450c11 gene, is expressed
in a tissue-specific, developmentally, and hormonally regulated
fashion in the rodent adrenal and encodes a protein with both
11-hydroxylase and 18-hydroxylase activities.
J. Biol. Chem. 270, 1643-1649 (1995)
CYP11B8P rat pseudogene
GenEMBL D14104 to D14108
Mukai,K., Imai,M., Shimada,H. and Ishimura,Y.
Isolation and characterization of rat CYP11B genes involved
in late steps of mineralo- and glucocorticoid syntheses.
J. Biol. Chem. 268, 9130-9137 (1993)
Note: authors call this sequence 11B4
12A Subfamily
CYP12A1 Musca domestica (housefly)
GenEMBL U86618
Rene Feyereisen
MIKYKQYSRAIVALRQRGAQQYSTNVTNASQPDVKATTTTTISP
EWQEAKPFEEMPSMNSWPIIKNMLPWGKYGKMEPTQFLMALRDDMGPIVRTAAFMGRP
PTVITHNPHDFEMVFRNEGIWPIRPGGDAQMYHRTVLREDFFQGVTGLVSVNGEKWGN
FRSTVNPVLMQPKNVRLYLNKMAQVNDEFMARIRQIRDPETLEVPASFQEEMNRWTLE
SVSVVALDKQLGLITTNRDNPDLKKLIGLLNDFFELGQKIEFGLPFWKYIKTPTFKLF
MKTLDGLLEIGNKYVNEAIDRLEAERQSGVPEKPENEKSVLEKLIKIDRKIATVMAID
MILAGVDTTSTTFTALLLCLAKNPEKQEKLREEIRQILPRKDSQFEPSSLNHIPYTRA
CIKEALRMYPLTLGNARILANDTVLSGYRVPKGTLVSMISTGLLQDDNHYTKAKEYLP
ERWMRPTKEETEDSATCPHALKASSPFIYLPFGFGPRSCVGRRIVEMELELGIARLVR
NFRIEFNYPTENAFKFKLINVPNIPLKFKFTDVEN
CYP12A2 Musca domestica (housefly)
GenEMBL U94698
Rene Feyereisen
CYP12A3 Musca domestica (housefly)
GenEMBL U94699
Rene Feyereisen
cyp12a4 Drosophila melanogaster
GenEMBL AC006091 85973-87865 also AC015190 AC008141
chromosome 3 clone BACR48G05 (D475)
MLKVRSALSLIQSQKATLSLATQKHTEYFKILLYIYINKLYYQRWQTNVATAEAREDSEW
LQAKPFEQIPRLNMWALSMKMSMPGGKYKNMELMEMFEAMRQDYGDIFFMPGIMGNPPFL
STHNPQDFEVVFRNEGVWPNRPGNYTLLYHREEYRKDFYQGVMGVIPTQGKPWGDFRTVV
NPVLMQPKNVRLYYKKMSQVNQEFVQRILELRDPDTLEAPDDFIDTINRWTLESVSVVAL
DKQLGLLKNSNKESEALKLFHYLDEFFIVSIDLEMKPSPWRYIKTPKLKRLMRALDGIQE
VTLAYVDEAIERLDKEAKEGVVRPENEQSVLEKLLKVDRKVATVMAMDMLMAGVDTTSST
FTALLLCLAKNPEKQARLREEVMKVLPNKNPEFTEASMKNVPYLRACIKESQRLHPLIVG
NARVLARDAVLSGYRVPAGTYVNIVPLNALTRDEYFPQASEFLPERWLRSPKDSESKCPA
NELKSTNPFVFLPFGFGPRMCVGKRIVEMELELGTARLIRNFNVEFNYPTENAFRSALIN
LPNIPLKFKFIDLPN
cyp12a5 Drosophila melanogaster
GenEMBL AC006091 83449-85296 also AC015190 AC008141
chromosome 3 clone BACR48G05 (D475)
76% identical to other AC006091 SEQ. 58% TO 12A1, 12A2
MLKGRIALNILQSQKPIVFSASQQ*RWQTNVPTAEIRNDPEWLQAKPFEE
IPKANILSLFAKSALPGGKYKNLEMMEMIDALRQDYGNIIFLPGMMGRDG
LVMTHNPKDFEVVFRNEGVWPFRPGSDILRYHRTVYRKDFFDGVQGIIPS
QGKSWGDFRSIVNPVLMQPKNVRLYFKKMSQVNQEFIKEIRDASTQEVPG
NFLETINRWTLESVSVVALDKQLGLLRESGKNSEATKLFKYLDEFFLHSA
DLEMKPSLWRYFKTPLLKKMLRTMDSVQEVTLKYVDEAIERLEKEAKEGV
VRPEHEQSVLEKLLKVDKKVATVMAMDMLMAGVDTTSSTFTALLLCLAKN
PEKQARLREEVMKVLPNKDSEFTEASMKNVPYLRACIKESQRVYPLVIGN
ARGLTRDSVISGYRVPAGTIVSMIPINSLYSEEYFPKPTEFLPERWLRNA
SDSAGKCPANDLKTKNPFVFLPFGFGPRMCVGKRIVEMELELGTARLIRN
FNVEFNHSTKNAFRSALINLPNIPLKFKFKFTDVPN*
CYP12A6 Drosophila wassermani
no accession number
Tina Yee and Phil Danielson
59% to 12A2 56% to 12A1
N-terminal does not match known P450 sequences may be in error
12B Subfamily
CYP12B1 Drosophila acanthoptera
no accession number
Phil Danielson
Ac40
submitted to nomenclature committee
Cyp12b2 Drosophila melanogaster
GenEMBL AC018326 7227-9141 also AC004345 AC004657
77% identical to 12b1
Cyp12c1 Drosophila melanogaster
GenEMBL AC009385 comp(57935-59646) also AC012807
Cyp12d1 Drosophila melanogaster
GenEMBL AC008187 comp(84371-86114)
Cyp12e1 Drosophila melanogaster
GenEMBL AC018294 2070-3932
CYP12F1 Anopheles gambiae (malaria vector)
No accession number
Submitted by Rene Feyereisen July 5, 2002
Low 40% range with other CYP12s 60% to 12F2
CYP12F1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPl3r4
CYP12F2 Anopheles gambiae (malaria vector)
No accession number
Submitted by Rene Feyereisen July 5, 2002
Low 40% range with other CYP12s 60% to 12F1
CYP12F2 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPl3r3
CYP12F3 Anopheles gambiae (malaria vector)
No accession number
Submitted by Rene Feyereisen July 5, 2002
Low 40% range with other CYP12s 60% to 12F2
CYP12F3 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPl3r2
CYP12F4 Anopheles gambiae (malaria vector)
No accession number
Submitted by Rene Feyereisen July 5, 2002
Low 40% range with other CYP12s 55% to 12F2
CYP12F4 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPl3r1
13A Subfamily
CYP13A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
Wilson,R., Ainscough,R., Anderson,K., Baynes,C., Berks,M.,
Bonfield,J., Burton,J., Connell,M., Copsey,T., Cooper,J.,
Coulson,A., Craxton,M., Dear,S., Du,Z., Durbin,R., Favello,A.,
Fulton,L., Gardner,A., Green,P., Hawkins,T., Hillier,L., Jier,M.,
Johnston,L., Jones,M., Kershaw,J., Kirsten,J., Laister,N.,
Latreille,P., Lightning,J., Lloyd,C., McMurray,A., Mortimore,B.,
O'Callaghan,M., Parsons,J., Percy,C., Rifken,L., Roopra,A.,
Saunders,D., Shownkeen,R., Smaldon,N., Smith,A., Sonnhammer,E.,
Staden,R., Sulston,J., Thierry-Mieg,J., Thomas,K., Vaudin,M.,
Vaughan,K., Waterston,R., Watson,A., Weinstock,L.,
Wilkinson-Sproat,J. and Wohldman,P.
2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
elegans.
Nature 368, 32-38 (1994)
Product T10B9.8
CYP13A2 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.7
CYP13A3 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.5
CYP13A4 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.1
CYP13A5 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.2
CYP13A6 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.3
CYP13A7 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.10
CYP13A8 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z83130 (ZK1325) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.4
CYP13A9P Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.6 pseudogene
Note: closer inspection suggests this may not be a pseudogene.
A complete gene can be assembled, but perhaps the genefinder program
did not see
it.
CYP13A10 Caenorhabditis elegans (nematode worm)
GenEMBL Z46934 (35989 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
product ZK1320.4
CYP13A11 Caenorhabditis elegans (nematode worm)
GenEMBL Z81503 (F14F7) and Z92819 (Y37D8) and Z94158 (Y39E4)
F14F7.a contig 666 10-13k region
CYP13A12 Caenorhabditis elegans (nematode worm)
GenEMBL Z81503 (F14F7) and Z92819 (Y37D8) and Z94158 (Y39E4)
F14F7.b contig 666 13-18k region
13B Subfamily
CYP13B1 Caenorhabditis elegans (nematode worm)
GenEMBL Z54269 (19692bp) and Z92827 (C29F7)
F02C12.5 also C29F7 has the end of this sequence
Formerly CYP16A1 but renamed after full length sequence sorted with
CYP13
CYP13B2 Caenorhabditis elegans (nematode worm)
GenEMBL Z81565
K06G5
14A Subfamily
CYP14A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z50742 (20856bp)
Wilson,R., Ainscough,R., Anderson,K., Baynes,C., Berks,M.,
Bonfield,J., Burton,J., Connell,M., Copsey,T., Cooper,J.,
Coulson,A., Craxton,M., Dear,S., Du,Z., Durbin,R., Favello,A.,
Fulton,L., Gardner,A., Green,P., Hawkins,T., Hillier,L., Jier,M.,
Johnston,L., Jones,M., Kershaw,J., Kirsten,J., Laister,N.,
Latreille,P., Lightning,J., Lloyd,C., McMurray,A., Mortimore,B.,
O'Callaghan,M., Parsons,J., Percy,C., Rifken,L., Roopra,A.,
Saunders,D., Shownkeen,R., Smaldon,N., Smith,A., Sonnhammer,E.,
Staden,R., Sulston,J., Thierry-Mieg,J., Thomas,K., Vaudin,M.,
Vaughan,K., Waterston,R., Watson,A., Weinstock,L.,
Wilkinson-Sproat,J. and Wohldman,P.
2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
elegans.
Nature 368 (6466), 32-38 (1994)
K09A11.2
CYP14A2 Caenorhabditis elegans (nematode worm)
GenEMBL Z50742 (20856bp)
see CYP14A1 for reference.
K09A11.3
CYP14A3 Caenorhabditis elegans (nematode worm)
GenEMBL Z50742 (20856bp)
see CYP14A1 for reference.
K09A11.4
CYP14A4 Caenorhabditis elegans (nematode worm)
GenEMBL Z50742 (20856bp) Z70212(R04D3.1 continuation of Z50742)
see CYP14A1 for reference.
after K09A11.5
partial sequence at end of cosmid continues on cosmid R04D3
CYP14A5 Caenorhabditis elegans (nematode worm)
GenEMBL U64847
F08F3.7
CYP15A1 cockroach
No accession number
Rene Feyereisen
CYP15B1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPj2l3
47% identical to 15A1
16A Subfamily
CYP16A1X Caenorhabditis elegans (nematode worm)
GenEMBL Z54269
number retired
This sequence is now CYP13B1
17A Subfamily
CYP17 human
GenEMBL NM_000102
MWELVALLLLTLAYLFWPKRRCPGAKYPKSLLSLPLVGSLPFLP
RHGHMHNNFFKLQKKYGPIYSVRMGTKTTVIVGHHQLAKEVLIKKGKDFSGRPQMATL
DIASNNRKGIAFADSGAHWQLHRRLAMATFALFKDGDQKLEKIICQEISTLCDMLATH
NGQSIDISFPVFVAVTNVISLICFNTSYKNGDPELNVIQNYNEGIIDNLSKDSLVDLV
PWLKIFPNKTLEKLKSHVKIRNDLLNKILENYKEKFRSDSITNMLDTLMQAKMNSDNG
NAGPDQDSELLSDNHILTTIGDIFGAGVETTTSVVKWTLAFLLHNPQVKKKLYEEIDQ
NVGFSRTPTISDRNRLLLLEATIREVLRLRPVAPMLIPHKANVDSSIGEFAVDKGTEV
IINLWALHHNEKEWHQPDQFMPERFLNPAGTQLISPSVSYLPFGAGPRSCIGEILARQ
ELFLIMAWLLQRFDLEVPDDGQLPSLEGIPKVVFLIDSFKVKIKVRQAWREAQAEGST
CYP17 human EST
GenEMBL Z19875 (235bp)
UK-HGMP (United Kingdom human genome mapping project)
covers amino acids 270-348 when translating the complementary
strand. The fragment goes through at least 6 frame shifts.
sequence ID AAAAWEO
CYP17 human EST
GenEMBL Z20209 (248bp)
UK-HGMP (United Kingdom human genome mapping project)
covers amino acids 265-349 when translating the complementary
strand. The fragment goes through at least 8 frame shifts.
sequence ID AAABPSZ
CYP17 human
GenEMBL S85459 (556bp)
Biason,A., Mantero,F., Scaroni,C., Simpson,E.R. and Waterman,M.R.
Deletion within the CYP17 gene together with insertion of foreign
DNA is the cause of combined complete 17
alpha-hydroxylase/17,20-lyase deficiency in an Italian patient
Mol. Endocrinol. 5, 2037-2045 (1991)
CYP17 rat
PIR A31359 (507 amino acids)
Namiki, M., Kitamura, M., Buczko, E. and Dufau, M.L.
Rat testis P-450-17-alpha cDNA: the deduced amino acid
sequence, expression and secondary structural configuration.
Biochem. Biophys. Res. Commun. 157, 705-712 (1988)
CYP17 rat
PIR D41425 (16 amino acids)
Imaoka, S., Kamataki, T. and Funae, Y.
Purification and characterization of six cytochromes P-450
from hepatic microsomes of immature female rats.
J. Biochem. 102, 843-851 (1987)
CYP17 rat
GenEMBL S50146 Z11902 (3345bp)
PIR S20655 (97 amino acids)
Nason,T.F., Han,X.G. and Hall,P.F.
Cyclic AMP regulates expression of the rat gene for steroid
17 alpha-hydroxylase/C17-20 lyase P-450 (CYP17) in rat Leydig
cells.
Biochim. Biophys Acta 1171, 73-80 (1992)
Note: have not been able to download S50146 from GCG or NCBI.
CYP17 rat
GenEMBL X69816 (7556bp)
Givens,C.R., Zhang,P., Bair,S.R. and Mellon,S.H.
Transcriptional regulation of rat cytochrome P450c17 expression in
mouse Leydig MA-10 and adrenal Y-1 cells: identification of a
single protein that mediates both basal and cAMP-induced activities.
DNA Cell Biol. 13, 1087-1098 (1994)
CYP17 rat
PIR S24316 (97 amino acids)
Zhang, P., Nason, T.F., Han, X.G. and Hall, P.F.
Gene for 17-alpha-hydroxylase/C(17-20) lyase P-450: complete
nucleotide sequence of the porcine gene and 5' upstream
sequence of the rat gene.
Biochim. Biophys. Acta 1131, 345-348 (1992)
CYP17 hamster
no accession number
Cloutier,M., Fleury,A., Courtemanche,J., Ducharme,L.
Mason,J.I. and Lehoux,J.G.
Cloning and expression of hamster adrenal cytochrome P450c17 cDNA.
Ann. N.Y. Acad. Sci. 774, 294-296 (1995)
CYP17 Cavia (guinea pig)
GenEMBL S75277(1732bp)
Tremblay,Y., Fleury,A., Beaudoin,C., Vallee,M. and Belanger,A.
Molecular cloning and expression of guinea pig cytochrome P450c17
cDNA (steroid 17 alpha-hydroxylase/17,20 lyase): tissue
distribution, regulation, and substrate specificity of the
expressed enzyme.
DNA Cell Biol. 13 (12), 1199-1212 (1994)
CYP17 Cavia porcellus (guinea pig)
PIR S52756 (508 amino acids)
Huang,Y., Voigt,J.M. and Colby,H.D.
unpublished
CYP17 pig
GenEMBL Z11854 to Z11856 GenEMBL S40341 (1858bp)
PIR S24233 (501 amino acids) PIR S30074 (501 amino acids)
Zhang,P. Nason,T.F., Han,X.G. and Hall,P.F.
Gene for 17 alpha-hydroxylasae/C(17-20) lyase P-450: complete
nucleotide sequence of the porcine gene and 5' upstream sequence of
the rat gene.
Biochim. Biophys. Acta 1131, 345-348 (1992)
CYP17 Sus scrofa (pig)
GenEMBL U41519 to U41525
Conley,A.J., Graham-Lorence,S.E., Kagimoto,M., Lorence,M.C.,
Murry,B.A., Oka,K., Sanders,D. and Mason,J.I.
Nucleotide sequence of a cDNA encoding porcine testis 17
alpha-hydroxylase cytochrome P-450.
Biochim. Biophys. Acta 1130, 75-77 (1992)
CYP17 bovine
GenEMBL M64646 (1725bp)
Zuber,M.X., John,M.E., Okamura,T., Simpson,E.R. and Waterman,M.R.
Bovine adrenocortical cytochrome P-450-17-alpha: Regulation of gene
expression by ACTH and elucidation of primary sequence
J. Biol. Chem. 261, 2475-2482 (1986)
CYP17 Ovis aries (sheep)
GenEMBL L40335 (1728bp)
Murry,B.A., Swart,P. and Mason,J.I.
Cloning and expression of ovine cytochrome P-450 17-alpha
hydroxylase/c17-20 lyase.
Unpublished (1995)
CYP17 Equus caballus (horse)
GenEMBL D30688(1906bp) D13818
Hasegawa,T., Mukoyama,H., Yoshida,S. and Takahashi,M.
Molecular cloning and nucleotide sequence of equine testicular
cytochrome P-450 steroid 17alpha-hydroxylase/C17,20-lyase messenger
ribonucleic acid.
Biol. Reprod. Mono. 1, 615-622 (1995)
CYP17 Equus caballus (horse)
GenEMBL D88184(6217bp)
Hasegawa,T.
Exon/Intron structure of Equine P450c17.
unpublished (1996)
CYP17 Oncorhynchus mykiss (rainbow trout)
GenEMBL S50356
Sakai,N., Tanaka,M., Adachi,S., Miller,W.L. and Nagahama,Y.
Rainbow trout cytochrome P-450c17 (17 alpha-hydroxylase/17-20
lyase) cDNA cloning, enzymatic properties and temporal pattern of
ovarian P-450c17 mRNA expression during oogenesis.
FEBS Lett. 301, 60-64 (1992)
Identical to X65800
CYP17 Oncorhynchus mykiss (rainbow trout)
GenEMBL X65800 (2287bp) Swiss P30437 (514 amino acids)
Sakai,N., Tanaka,M., Adachi,S., Miller,W.L. and Nagahama,Y.
Rainbow trout cytochrome P-450c17 (17
alpha-hydroxylase/17,20-lyase). cDNA cloning, enzymatic properties
and temporal pattern of ovarian P-450c17 mRNA expression during
oogenesis
FEBS Lett. 301, 60-64 (1992)
Identical to S50356
CYP17 Oryzias latipes (medaka)
GenEMBL D87121(2421bp)
Kobayashi,D., Matsuyama,M., Tanaka,M., Fukada,S. and Nagahama,Y.
Structural analysis of medaka P-450c17 and expression in the
ovarian follicle.
unpublished (1996)
CYP17 Oryzias latipes (medaka)
GenEMBL D87122(2302bp)
Kobayashi,D., Tanaka,M., Fukada,S. and Nagahama,Y.
Presence of a Novel Cytochrome P-450c17 Transcripts in Medaka
Gonads
unpublished (1996)
note: this sequence is missing exon 6
otherwise identical to D87121
CYP17 Squalus acanthias (dogfish)
GenEMBL S77384 (1964bp)
Trant,J.
Isolation and characterization of the cDNA encoding the spiny
dogfish shark
(Squalus acanthias) form of cytochrome P450c17
J. Exp. Zool. 272, 25-33 (1995)
CYP17 Ictalurus punctatus (channel catfish)
GenEMBL AF063837
Trant,J.M., Berard,C., Byrne,B.J. and Wunder,J.
Isolation and heterologous expression of the cDNA encoding the
cytochrome P450 17-hydroxylase from the channel catfish (Ictalurus
punctatus)
Unpublished (1998)
CYP17A1 Fugu rubripes (pufferfish)
No accession number
Scaffold_4175
80% to Oryzias latipes 17, 79% to trout 17 73% to catfish
61% to dogfish 48% to other CYP17 from Fugu
MDWVLFVYAFSAVNLALLALHLKFRTPASGPRGPPRLPALPLIGSLLSLRSPHPPHVLFKE
LQGKYGQTYSLMMGSHRVIIVNHHAHAKEVLLKKGKIFAGRPRS 11667 (0)
11572 VTTDVLSRDGKDIAFGDYSATWRFHRKIVHGALCMFGEGSASIEKI 11435 (1)
10871 ICAEAASLCSILSEAWTAGLALDLSPELTRAVTNVICSLCFSSSYRRGDAEFEAMLHYSQ 10692
10691 GIVDTVAKDSLVDIFPCLQ 10635 (0)
IFPNADLRLLKRCVSVRDKLLQKEYDKHK (0)
AAYSDHVQRDLLDALLRAKCSAENNNTTGINAESVGLTDDHLLMT
VGDIFGAGVETTTTVMKWAITYLIHHPQ 9765 (0)
9637 IQSRIQEELDSRVGMDRSPQLSDRGSLPYLEATIREVLRIRPVAPLFIPHVALSDT 9473 (2)
9369 SIGDFAVKKGTRVVINLWSLHHDEKEWENPERFDP 9265 (1)
GRFLNSEGTGLVIPSSSYLPFGAGVRVCLGEALAK 8558
8557 MELFLFLSWILQRFTLTVPSGHSLPSLEGKFGVVLQPTKYKVNATPRPGWEGKCKACWN* 8378
CYP17A2 Fugu rubripes (pufferfish)
No accession number
Scaffold_8086
50% to Oryzias latipes 17, 48% to trout 17 50% to catfish
49% to dogfish 48% to other CYP17 from Fugu
1198 MVTVGSFLIFRRPVRGSEPGSEAGPPRVKVPCISWVPVLGSLPWLRGGRPLHLIFTQLSYR 1380 (2)
1600 YGPLFALYLGPHLTVVVNNHQHAREVLLLRGKDFAGRPRM 1719 (0)
1797 VTTDLLTRGGKDIAFSDYCPLWKSHRRLVQNSFTLFGEGTSRLQDM 1934 (1)
2061 VLAAVDSLCEELLSMEGRGFDPAPAVTRAVTNVVCMLVFSATYRHGDSELQEVLRYNDGI 2240
2241 VQTIAGGGLVDIYPWMK 2291 (0)
2372 VFPNKTLSKLKACIAVRDRLLTHKLEEHK 2458 (0)
2622 ATLTDNQPRDLLDALLMGQVGRGRRKGSGRVEEDIITEDHVLMTAAEAFGAGVETTSTTLLWILAYLLHHPQ 2837 (0)
2925 VQERVQKELDDHVGSERPVRVSDRARLTYLDCVINEGMRIRPVSPVLIPHTAMTDSR 3074 (2)
3870 IGGHHISRGTRVLVNMWSIHHDSAHWDKPDLFNP 3971 (1)
4519 DRFRDHQGQRVTPSCFLPFGAGPRVCVGESLARLELFLFLSSLLQRMSFRLPNGA 4692
4693 SPPDLQGRMGVVLQPVPYKVVVTPRVG* 4776
CYP17 fragment a Fugu rubripes (pufferfish)
No accession number
Fc:c028I22x2 LPC.10549.x2 Length = 1007 61% to 17A1 Fugu
GAPRATTDNHHAHAKEARPKKGKKAAGRPRK exon 1
ATTDGASRDGTDTAEGDHSATRRDQR exon 2
CYP17 fragment b Fugu rubripes (pufferfish)
No accession number
Fc:c028I22x1 LPC.10549.x1 Length = 894
78% to Fc:c028I22x2 65% to 17A1 Fugu
KEQQAEHGQTTSRMMGSHRGNTDNQHAHAKEARQKKGKKGAGRPRK exon 1
ATTDGRSRDGTDIAEGDHSATWRHHRKIVQRARRRNGEGSAPSEKI exon 2 C-helix
18A Subfamily
Cyp18 Drosophila melanogaster
GenEMBL S66112 (63bp)
Hurban,P. and Thummel,C.
Isolation and characterization of fifteen ecdysone-inducible
drosophila genes reveal unexpected complexities in ecdysone regulation.
Molec. Cell Biol. 13, 7101-7111 (1993)
Note: very short ecdysone inducible fragment in the heme binding
region about 2/3 of amino acids are identical to 2D sequences.
called Eig 17-1
dimethylnitrosamine demethylase
Cyp18 Drosophila melanogaster
GenEMBL U44753(2539bp)
Bassett,M.H, Waterman,M.R., McCarthy,J.L. and Sliter,T.J.
Cloning and characterization of CYP18 in Drosophila melanogaster:
identification of an insect member of a new cytochrome P450 family.
unpublished (1996)
complete sequence from the Eig 17-1 fragment
Cyp18 Drosophila melanogaster
GenEMBL AC012164 114600-117669 also AC015216
CYP18 Spodoptera littoralis (cotton leafworm)
No accession number
Lyndsay Davies
Submitted to nomenclature committee 2/22/02
61% identical to CYP18 from Drosophila
since there is only one CYP18 seq in Drosophila this new sequence
will be called CYP18 without a subfamily or number like CYP18A1.
If more CYP18s are found in a single species this may have to change.
19A Subfamily
CYP19 human
GenEMBL S52034 (142bp) S52789 (106bp) S52793 (149bp)
S52794 (125bp)
Harada,N.
A unique aromatase (P-450AROM) mRNA formed by alternative
use of tissue-specific exons 1 in human skin fibroblasts.
Biochem. Biophys. Res. Commun. 189, 1001-1007 (1992)
CYP19 human
GenEMBL D14473 (295bp) S59092 S59095 S59171
Toda,K and Shizuta,Y.
Molecular cloning of a cDNA showing alternative splicing of
the 5'-untranslated sequence of mRNA for human aromatase P-450.
Eur. J. Biochem. 213, 383-389 (1993)
CYP19 human
GenEMBL D13391 (2238bp)
Katsumi,T. and Shizuta,Y.
Identification and characterization of cis-acting regulatory
elements for
the expression of the human aromatase cytochrome P-450 gene.
J. Biol. Chem. 269, 8099-8107 (1994)
CYP19 human
GenEMBL D21240 (794bp) D21241 (3231bp)
Harada,N., Utsumi,T. and Takagi,Y.
Tissue-specific expression of the human aromatase cytochrome P-450
gene by alternative use of multiple exons 1 and promoters, and
switching of tissue-specific exons 1 in carcinogenesis.
Proc. Natl. Acad. Sci. U.S.A. 90 (23), 11312-11316 (1993)
CYP19 human
GenEMBL X55983 (669bp)
Toda,K., Miyahara,K., Kawamoto,T., Ikeda,H., Sagara,Y. and
Shizuta,Y.
Characterization of a cis-acting regulatory element involved in
human-
aromatase P-450 gene expression.
Eur, J. Biochem. 205, 303-309 (1992)
exon 1
CYP19 human
GenEMBL S71536 (792bp)
Toda,K., Simpson,E.R., Mendelson,C.R., Shizuta,Y. and Kilgore,M.W.
Expression of the gene encoding aromatase cytochrome P450 (CYP19)
in fetal tissues
Mol. Endocrinol. 8, 210-217 (1994)
CYP19 human
GenEMBL M32245 (840bp)
Harada,N., Yamada,K., Saito,K., Kibe,N., Dohmae,S. and Takagi,Y.
Structural characterization of the human estrogen synthetase
(aromatase) gene.
Biochem. Biophys. Res. Commun. 166, 365-372 (1990)
CYP19 human
GenEMBL D29757 (875bp) PIR PC2041 (45 amino acids)
Honda,S.-I., Harada,N. and Takagi,Y.
Novel exon 1 of the aromatase gene specific for aromatase
transcripts
in human brain.
Biochem. Biophys. Res. Commun. 198, 1153-1160 (1994)
CYP19 human
GenEMBL M22246 (2966bp)
Harada,N.
Cloning of a complete cDNA encoding human aromatase: immunochemical
identification and sequence analysis
Biochem. Biophys. Res. Commun. 156, 725-732 (1988)
CYP19 human
GenEMBL L21982 (1166bp)
Mahendroo,M.S., Mendelson,C.R. and Simpson,E.R.
Tissue-specific and hormonally-controlled alternative promoters
regulate aromatase cytochrome P450 gene expression in human adipose
tissue
J. Biol. Chem. 268, 19463-19470 (1993)
CYP19 human
GenEMBL S85356 (1384bp)
Means,G.D., Kilgore,M.W., Mahendroo,M.S., Mendelson,C.R. and
Simpson,E.R.
Tissue-specific promoters regulate aromatase cytochrome P450 gene
expression in human ovary and fetal tissues.
Mol. Endocrinol. 5, 2005-2013 (1991)
CYP19 human
GenEMBL S96437 (971bp)
Kilgore,M.W., Means,G.D., Mendelson,C.R. and Simpson,E.R.
Alternative promotion of aromatase P-450 expression in the human
placenta.
Mol. Cell. Endocrinol. 83, R9-R16 (1992)
CYP19 human
PIR A40542 (48 amino acids)
Mahendroo, M.S., Means, G.D., Mendelson, C.R. and Simpson, E.R.
Tissue-specific expression of human P-450-AROM. The promoter
responsible for expression in adipose tissue is different
from that utilized in placenta.
J. Biol. Chem. 266, 11276-11281 (1991)
CYP19 Macaca fuscata (Japanese macaque)
GenEMBL S79807(369bp)
Yamada-Mouri,N., Hirata,S., Hayashi,M. and Kato,J.
Analysis of the expression and the first exon of aromatase mRNA in
monkey brain.
J. Steroid Biochem. Mol. Biol. 55 (1), 17-23 (1995)
CYP19 rat
GenEMBL S59505 (639bp)
Fitzpatrick,S.L. and Richards,J.S.
cis-acting elements of the rat aromatase promoter required for
adenosine 3',5'-monophosphate induction in ovarian granulosa cells
and constitutive expression in R2C Leydig cells.
Molec. Endocrinol. 7, 341-354 (1993)
Note: promoter
CYP19 rat
GenEMBL Z11815 (590bp)
Hickey,G.J., Krasnow,J.S., Beattie,W.G. and Richards,J.S.
Aromatase cytochrome P450 in rat ovarian granulosa cells before and
after luteinization: Adenosine 3',5'-monophosphate-dependent and
independent regulation. Cloning and sequencing of rat aromatase
cDNA and 5' genomic DNA
Mol. Endocrinol. 4, 3-12 (1990)
CYP19 Oryctolagus cuniculus (rabbit)
GenEMBL Z68271(1783bp)
Delarue,B., Mittre,H., Feral,C., Benhaim,A. and Leymarie,P.
Rapid sequencing of rabbit aromatase cDNA using RACE PCR without
cloning.
C. R. Acad. Sci. III, Sci. Vie 319, 663-670 (1996)
CYP19 Oryctolagus cuniculus (rabbit)
GenEMBL Z70302(1455bp)
Delarue,B., Mittre,H. and Leymarie,P.
Expression des transcrits codant pour l'aromatase de lapin dans
differents tissus.
unpublished (1996)
Cyp19 mouse
Swiss P28649 (503 amino acids) GenEMBL D00659 (2420bp)
Terashima,M., Toda,K., Kawamoto,T., Kuribayashi,I., Ogawa,Y.,
Maeda,T. and Shizuta,Y.
Isolation of a full-length cDNA encoding mouse aromatase P450
Arch. Biochem. Biophys. 285, 231-237 (1991)
CYP19 pig
no accession number
Corbin, C.J., Khalil, M.W. and Conley, A.J.
Functional ovarian and placental isoforms od porcine aromatase.
Mol. Cell. Endocrinol. 113, 29-37 (1995)
CYP19 Sus scrofa (pig)
GenEMBL L15471 (454bp)
Ko,Y., Choi,I., Green,M.L., Simmen,F.A. and Simmen,R.C.
Transient expression of the cytochrome P450 aromatase gene in
elongating porcine blastocysts is correlated with uterine
insulin-like growth factor levels during peri-implantation
development.
Mol. Reprod. Dev. 37 (1), 1-11 (1994)
Note: This is only a fragment of 80 amino acids, including
helix K and the EXXR conserved sequence.
CYP19 Sus scrofa (pig)
GenEMBL U52141(1133bp)
Choi,I., Collante,W., Simmen,R.C.M. and Simmen,F.A.
Molecular cloning of multiple forms of cytochrome p450 aromatase
and their developmental expression in porcine blastocysts,
endometrium, and placenta.
Unpublished (1997)
CYP19 Sus scrofa (pig)
GenEMBL U52142(1584bp)
Choi,I., Collante,W., Simmen,R.C.M. and Simmen,F.A.
Molecular cloning of multiple forms of cytochrome p450 aromatase
and their developmental expression in porcine blastocysts,
endometrium, and placenta.
Unpublished (1997)
CYP19 Sus scrofa (pig)
GenEMBL U37309 (417bp)
Choi,I., Simmen,R.C. and Simmen,F.A.
Molecular cloning of cytochrome P450 aromatase complementary
deoxyribonucleic acid from periimplantation porcine and equine
blastocysts identifies multiple novel 5'-untranslated exons
expressed in embryos, endometrium, and placenta.
Endocrinology 137 (4), 1457-1467 (1996)
CYP19 Sus scrofa (pig)
GenEMBL U37311(2470bp)
Choi,I., Simmen,R.C. and Simmen,F.A.
Molecular cloning of cytochrome P450 aromatase complementary
deoxyribonucleic acid from periimplantation porcine and equine
blastocysts identifies multiple novel 5'-untranslated exons
expressed in embryos, endometrium, and placenta.
Endocrinology 137 (4), 1457-1467 (1996)
CYP19 Sus scrofa (pig)
GenEMBL U57510
Choi,I., Collante,W., Simmen,R.C.M., Troyer,D. and Simmen,F.A.
Molecular cloning and structural characterization of porcine
cytochrome p450 aromatase chromosomal genes: evidence for the
existence of multiple, closely related genes that encode
developmental and tissue-specific isoforms of aromatase.
unpublished (1997)
CYP19 Sus scrofa (pig)
GenEMBL U57517(287bp) U57518(287bp) U57519(358bp)
Choi,I., Collante,W., Simmen,R.C.M., Troyer,D. and Simmen,F.A.
Molecular cloning and structural characterization of porcine
cytochrome p450 aromatase chromosomal genes: evidence for the
existence of multiple, closely related genes that encode
developmental and tissue-specific isoforms of aromatase.
unpublished (1997)
CYP19 Sus scrofa (pig)
GenEMBL U57520(517bp) U57521(495bp)
Choi,I., Collante,W., Simmen,R.C.M., Troyer,D. and Simmen,F.A.
Molecular cloning and structural characterization of porcine
cytochrome p450 aromatase chromosomal genes: evidence for the
existence of multiple, closely related genes that encode
developmental and tissue-specific isoforms of aromatase.
unpublished (1997)
CYP19 bovine
GenEMBL S66248 (2104bp)
Hinshelwood,M.M., Corbin,C.J., Tsang,P.C. and Simpson,E.R.
Isolation and characterization of a cDNA insert encoding bovine
aromatase
cytochrome P450.
Endocrinology 133, 1971-1977 (1993)
Note two amino acid differences with Z32741
CYP19 bovine
GenEMBL M64646 (1725bp)
Zuber,M.X., John,M.E., Okamura,T., Simpson,E.R. and Waterman,M.R.
Bovine adrenocortical cytochrome P-450-17-alpha: Regulation of gene
expression by ACTH and elucidation of primary sequence.
J. Biol. Chem. 261, 2475-2482 (1986)
CYP19 bovine
GenEMBL Z32741 (4226bp) PIR S44210 (503 amino acids)
Vanselow,J. and Furbass,R.
Aromatase cytochrome P450 gene and pseudogene
unpublished (1994)
CYP19 bovine
GenEMBL Z69241 to Z69250 (genomic sequences)
Furbass,R. and Vanselow,J.
unpublished (1996)
CYP19P bovine
GenEMBL Z32813 (1006bp)
Vanselow,J. and Furbass,R.
Aromatase cytochrome P450 gene and pseudogene
unpublished (1994)
CYP19 Equus caballus (horse)
GenEMBL U37313 (458bp)
Choi,I., Simmen,R.C. and Simmen,F.A.
Molecular cloning of cytochrome P450 aromatase complementary
deoxyribonucleic acid from periimplantation porcine and equine
blastocysts identifies multiple novel 5'-untranslated exons
expressed in embryos, endometrium, and placenta.
Endocrinology 137 (4), 1457-1467 (1996)
CYP19 chicken (three different strains of chicken)
GenEMBL M73277 to M73285, M73286 to M73294
M73295 to M73303
PIR A41063 (495 amino acids)
Matsumine,H., Herbst,M., Ou,S.-H.I., Wilson,J.D. and
McPhaul,M.J.
Aromatase mRNA in the extragonadal tissues of chickens with
the henny-feathering trait is derived from a distinctive
promoter structure that contains a segment of a retroviral
long terminal repeat.
J. Biol. Chem. 266, 19900-19907 (1991)
CYP19 Coturnix coturnix japonica (Japanese quail)
GenEMBL S46949 (692bp) PIR A48977(230 amino acids)
Harada,N., Yamada,K., Foidart,A. and Balthazart,J.
Regulation of aromatase cytochrome P-450 (estrogen synthetase)
transcripts in the quail brain by testosterone.
Brain Res. Mol. Brain Res. 15, 19-26 (1992)
Note: only three amino acid differences with chicken.
CYP19 Coturnix coturnix (quail)
GenEMBL D50336(4351bp)
Kudo,T., Yamamoto,H., Sato,S. and Sutou,S.
Comparison of 5' upstream regions of chicken and quail aromatase
gene.
Unpublished (1995)
CYP19 Poephila guttata (zebra finch)
GenEMBL S75898(3188bp)
Shen,P., Campagnoni,C.W., Kampf,K., Schlinger,B.A., Arnold,A.P. and
Campagnoni,A.T.
Isolation and characterization of a zebra finch aromatase cDNA: in
situ hybridization reveals high aromatase expression in brain
Brain Res. Mol. Brain Res. 24 (1-4), 227-237 (1994)
CYP19 Ictalurus punctatus (channel catfish)
GenEMBL S75715(2102bp)
Trant,J.
Isolation and characterization of the cDNA encoding the channel
catfish (Ictalurus punctatus) form of cytochrome P450arom
Gen. Comp. Endocrinol. 95 (2), 155-168 (1994)
CYP19 Onchorynchus mykiss (rainbow trout)
Tanaka, M., Telecky, T.M., Fukada, S., Adachi,S., Chen, S. and
Nagahama, Y.
Cloning and sequence analysis of the cDNA encoding P-450 aromatase
(P450arom) from a rainbow trout (Onchorynchus mykiss) ovary,
relationship
between the amount of P450arom mRNA and the production of
oestradiol-17-beta
in the ovary.
J. Mol. Endocrin. 8, 53-61 (1992)
CYP19 Carassius auratus (goldfish)
GenEMBL U18974(2939bp)
Gelinas,D.M., Pitoc,G.A. and Callard,G.V.
Isolation of goldfish brain aromatase cDNA and analysis of
expression during the reproductive cycle and after steroid
treatment in vivo.
unpublished (1996)
CYP19 Oryzias latipes (medaka)
GenEMBL D82968(1851bp)
Tanaka,M., Fukada,S., Matsuyama,M. and Nagahama,Y.
Structure and promoter analysis of the cytochrome P-450 aromatase
gene of the teleost fish, medaka (Oryzias latipes)
J. Biochem. 117 (4), 719-725 (1995)
CYP19 Tilapia nilotica (Cichlid fish)
GenEMBL U72071(1804bp)
Chang,X.T., Kobayashi,T., Nakamura,M., Kajura,H. and Nagahama,Y.
Isolation and characterization of cDNA encoding the tilapia
(Oreochromis niloticus) cytochrome P450 aromatase (P450arom):
Changes in P450arom mRNA, protein and enzyme activity in ovarian
follicles during oogenesis.
J. Mol. Endocrinol. (1996) In press
CYP19 Haplochromis burtoni (cichlid fish)
GenEMBL AF114716
White,R.B.
Aromatase expression during social change in the cichlid fish,
Haplochromis burtoni.
Unpublished
CYP19 Paralichthys olivaceus (Japanese Flounder)
GenEMBL AB017182
Kitano,T., Takamune,K., Kobayashi,T., Nagahama,Y. and Abe,S.
Suppression of P450 Aromatase (P450arom) Gene Expression in
Sex-Reversed Males Produced by Rearing Genetically Female Larvae at
High Water Temperature during a period of Sex Differentiation in
Japanese Flounder (Paralichthys olivaceus)
Unpublished (1998)
CYP19A1 Fugu rubripes (pufferfish)
No accession number
Scaffold_7098
65% to AF183906 ovary form of CYP19 from Zebrafish
60% to AF183908 brain form of CYP19 from Zebrafish
61% to other Fugu CYP19
this is probably the ovary form
9466 MAAVGLDAEVLVSVSPNATEAESPGSSAGTRALIILTCLLLLVWSHTEKKSVP 9308 (1)
9242 SLLGPSFCLGFGPLLTYVRFIWTGIGTASNYYNKKYGDIVRVWVNGEETLVISR 9081 (2)
8985 ASAVHHVLKSRQYTSRFGSKQGLSCIGMNERGIIFNNNVTEWRKIRGYFTK 8830 (1)
8759 ALTGPAVQNTVEVCNSSTQAHLDRLEDLAQVDVLSLLRCTVVDISNRLFLDIPIN 8595 (1)
8499 EKELLLKIHKYFDTWQTVLIKPDIYFKFGWIHQKHKTAA 8392 (2)
8296 RELQEAIEGLVEQKRRDLEQADKLENINFTAELLFAQ 8186 (0)
8084 NHGELSAENVMQCVLEMVIAAPDTLSVSLFFMLLLLKQNPDVELQLLQEIDAVVGK (0 expected, bad boundary)
RQLQNGDLQKLRVLETFINECLRFHPV 7719
7718 VDFTMRRSLSDDVIEGYRVPKGTNIILNTGHMHRTEFFLRPTEFCLQNFEKN 7563 (0)
APRRYFQPFGSGPRACVGKHIAMVMMKSILVTLLSQYSVCPHEGLT 7327
7326 LDCLPQTNNLSQQPVEHQEEAQQLSMRFLPRQRGSWQTV* 7207
CYP19A1 Danio rerio (zebrafish)
GenEMBL AF183906
Chiang,E.F.L., Yan,Y.L., Guiguen,Y., Postlethwait,J. and Chung,B.C.
Two Cyp19 (P450 Aromatase) Genes on Duplicated Zebrafish
Chromosomes Are Expressed in Ovary or Brain
Mol. Biol. Evol. 18 (4), 542-550 (2001)
Called CYP19a expressed in ovary
CYP19A2 Fugu rubripes (pufferfish)
No accession number
Scaffold_4200
65% to both brain and ovary forms of CYP19 from Zebrafish
61% to other Fugu CYP19
this is probably the brain form
2918 MKPKETLNITASGPFTPLPLPLMMMMMMLLLMMMMLFLTWNRPQRQHVP (1)
3451 GPLFLAGLGPLLSYCRFMWTGIGTACNFYNNKYGSLVRVWINGEETLILSR (2)
3674 SSAVYHVLRSAHYTARFGSRAGLECIGMEGQGVIFNSDVQLWRRARVYFSK (1)
3960 ALTGPGLQRTVGVCVTSTAKHLDCLVDMTDASGHVDALNLLRAIVVDISNRLFLRVPLN 4136 (1)
EKDLLTKIHNYFETWQAVLIKPDIFFKIGWLFDKHRRAA 4319 (2)
QELQDTMAALLKVKRKLVHEAEKLDDVLDFATELILAQ (0)
EAGEFSADNVRQCVLEMVIAAPDTLSISLFFMLMLLKQHPDVELRIVEELSTvsrt (0)
egeENIDYQRLKVMESFINESMRFHPVVDFTMRKALEDDTIEGIRIRKGTNIILNIGLMHKTE 5039
5040 FFPKPREFSLTNFEQT (0)
VPSRFFQPFGCGPRSCVG 5219
5220 KHIAMVMMKAILATLLSRYTVCPRHGCTLTSIRQTNNLSQQPVEDEHSLAMRFIPRTIQSPS* 5408
CYP19A1 Danio rerio (zebrafish)
GenEMBL AF183908
Chiang,E.F.L., Yan,Y.L., Guiguen,Y., Postlethwait,J. and Chung,B.C.
Two Cyp19 (P450 Aromatase) Genes on Duplicated Zebrafish
Chromosomes Are Expressed in Ovary or Brain
Mol. Biol. Evol. 18 (4), 542-550 (2001)
Called CYP19b expressed in brain
CYP19 Trachemys scripta (red-eared slider turtle)
no accession number
Crews,D.
Temperature-dependent sex determination: the interplay of steroid
hormones
and temperature.
Zool. Sci. 13, 1-13 (1996)
CYP20 human
GenEMBL AC011737.8 chromosome 2 clone RP11-33N4,
AC011737.8 chr 2 (missing exons 12,13)
AC080075.2 (missing exons 1,7,8)
MLDFAIFAVTFLLALVGAVLYLYP (0)
ASRQAAGIPGITPTEEK (2)
DGNLPDIVNSGSLHEFLVNLHERYGPVVSFWFGRRLVVSLGTVDVLKQHINPNKTS (1)
DPFETMLKSLLRYQSGGGSVSENHMRKKLYENGVTDSLKSNFALLLK (0)
LSEELLDKWLSYPETQHVPLSQHMLGFAMKSVTQMVMGSTFEDDQEVIRFQKNHGT (0)
VWSEIGKGFLDGSLDKNMTRKKQYED (1)
ALMQLESVLRNIIKERKGRNFSQHIFIDSLVQGNLNDQQ (0)
ILEDSMIFSLASCIITAK (1)
LCTWAICFLTTSEEVQKKLYEEINQVFGNGPVTPEKIEQLR (2)
YCQHVLCETVRTAKLTPVSAQLQDIEGKIDRFIIPRE (0)
TLVLYALGVVLQDPNTWPSPHK (2) genomic seq stops here the rest is cDNA
FDPDRFDDELVMKTFSSLGFSGTQECPELR (2) intron site based on fish genomic DNA
FAYMVTTVLLSVLVKRLHLLSVEGQVIETKYELVTSSREEAWITVSKRY
CYP20 mouse
GenEMBL AK020848 adult retina cDNA plus ESTs for C-term
MLDFAIFAVTFLLALVGAVLYLYPASRQASGIPGLTPTEEKDGN
LPDIVNSGSLHEFLVNLHERYGPVVSFWFGRRLVVSLGTTDVLKQHFNPNKTSDPFET
MLKSLLGYQSGGGSAGEDHVRRKLYGDAVTASLHSNFPLLLQLSEELLDKWLSYPETQ
HIPLSQHMLGFALKFVTRMVLGSTFEDEQEVIRFQKIHG
TVWSEIGKGFLDGSLDKNTTRKKQYQEALMQLESTLKKIIKERKGGNFRQHT
FIDSLTQGKLNEQQILEDCVVFSLASCIITAR
LCTWTIHFLTTTGEVQKK
LCKEIDQVLGEGPITSEKIEQLSYCQQVLFETVRTAKLTPVSARLQDIEGKVGPFVIPKE 360
TLVLYALGVVLQDPSTWPLPHRFDPDRFADEPVMKVFSSLGFSGTWECPELXFAYMVTAV 540
LVSVLLEKLRLLAVDRQVVEMKYELVTSAREEAWITVSKRH*
CYP20 Bos taurus (cow)
MLDFAIFAVTFLLALVGAVLYLYPASRQAAGIPGITPTEEKDGNLPDIV
NSGSLHEFLVNLHERYGPVVSFWFGRRLVVSLGTVDVLKQHINPNKTLDPFETMLKSLLR
YQSDSGNVSENHMRKKLYENGVTNCLRINFALLIKLSEELLDKWLSYPESQHVPLCQHML
GFAMKSVTQMVMGSTFEDEQEVIRFQKNHGTVWSEIGKGFLDGSLDKSTTRKKQYEDALM
QLESILKKIKERKGRNFSQHIFIDSLVQGNLNDQQILEDTMIFSLAS
CMITAKLCTWAVCFLTTYEEIQKKLYEEIDQVLGKGPITSEKIEELRYCRQVLCETVRTA
KLTPVSARLQDIEGKIDKFIIPRETLVLYALGVVLQEXGTWSSPYKFDPERFDDESVMKT
FSLLGFSGTRECPELRFAYMVTAVLLSVLLRRLHLLSVE
GQVIETKYELVTSSKEEAWITVSKRY 498
CYP20 Gallus gallus (chicken)
BU454844 603215388F1 CSEQRBN14 Gallus gallus cDNA clone ChEST201o21 5'.
BU111630 603125978F1 CSEQCHL13 Gallus gallus cDNA clone ChEST95k19 5'
BU356654 603474052F1 CSEQCHN70 Gallus gallus cDNA
MLDFAIFAVTFLLILVGAVLYLYP
ASRQASGIPGLAPTDDK
DGNLPDIIASRSLHEFLVNLHEKYGPLVSFWFGRRLVVSLGSIDLLKQHVNPNRSS
DPFEMMLKSFLRYQSSLNGDTGESHLRRKLYESGVSKSLQSNLALIQK
LSEELLAKWLSLPEAQHIPLCQHMLGFAMKSVTQTAMGSSFEDDQEVIRFRRHHDA
IWSEIGKGFLDGSLDKNATRKKLYED
ALKEMESTLRKVITGTPRQIIQQAFIDTLLQGNLSDQQ
ILEDTMIFSLAGCIITAN
LCTWAVYFLTTSEDVQQNLCKEVDHVLGKGPITHEKIEQLR
YCRQVLCETVRTAKLTPIAAQLQELEGRVDQHTVPKE
TLVLYALGVMLQDSSSWPSPYK
FDPERFSEDSAMTNFSLLGFSGSQECPELR
FAYMVATVLLSILVRKLYLHPVKGQVMETKYELVTSPKEEAWITVSKRS*
CYP20 Xenopus laevis (African clawed frog)
BJ037591.1 NIBB Mochii normalized Xenopus neurula library Xenopus laevis cDNA clone XL040p15 5'
BQ725586 BQ725586.1 AGENCOURT_8103453 NICHD XGC Emb2 Xenopus laevis cDNA clone
95% identical to tropicalis seq. 74% to chicken
BC044111.1 mRNA, complete cds Length = 2417
175 MLDFAIFAITFLLILVGAVLYLYPSSRQACGIPGLAPTEEKDGNLQDIVNSGS 333
334 LHEFLVNLHERFGPVASFWFGRRLVVSLGSLDLLKQHINPNKTSDPFQTMLKSLLGYQSG 513
514 VIGEAAESHVQKKLYENGITKALHSNFSVIIKLSEELLAKWGTYPQSQHVPLCQHMLGFA 693
694 MKSVTQTAMGSSFEDDQEVIHFRRNHDAIWSEIGKGFLDGSIERSPSRKKLYEDALMEME 873
874 TVLKKTIKERKGKNPGRHVFLDSLLQGNLSDKQVLEDSMIFSLAGCVITANLCTWAIYFL 1053
1054 TTSEEVQDKLYKEVNRVIGKGPITMDKLEQLSYCRQILCETVRTASLTPISARLQELEGR 1233
1234 VDQHIIPKETLVLYALGVVLQDNTAWPLAYRFDPDRFDDETAKQSLSLLGLSGSQECPEL 1413
1414 RFAYMVAMVLLCVLVRKLNLLPVKGQVMETKYELVTSPKEEAWITVSKRS 1563
CYP20 Xenopus tropicalis
From JGI blast server http://aluminum.jgi-psf.org/prod/bin/runBlast.pl?db=xenopus1&dump=1
And from Sanger http://www.sanger.ac.uk/Projects/X_tropicalis/blast_server.shtml
AL848968.1 Xenopus tropicalis EST, clone TEgg007o12 5'
AL870093 AL870093.1 Xenopus tropicalis EST, clone TEgg120l22 5'
60% to Fugu CYP20, 70% to human CYP20, 62% to zebrafish, 30% to ciona CYP20 like seq
72% to chicken
MLDFAIFAITFLLILVGAVLYLYP exon 1
SSRQACGIPGLAPTEEK exon 2
DGNLQDIVNSGSLHEFLVNLHERFGPVASFWFGRRLVVSLGSLDLLKQHINPNKTS exon 3
DPFQMMLKSLLGYQSGVIGEAAESHVQKKLFENGIIKALHSNFSVVIK exon 4
LSEDLLAKWLTYPQSQHVPLCQHMLGFAMKSVTQTAMGSSFDDDQEVIHFRRNHDA exon 5
IWSEIGKGFLDGSIERSPNRKKLYED exon 6
ALMEMETVLKKAIKERKVKNPGRHVFVDSLLQGNLSDKQ exon 7
VLEDSMIFSLAGcvitan exon 8
VCTWAIYFLTTSEEVQDKLFKEVTRVIGKGPITMDKLEQLS exon 9
YCRQILCETVRTASLTPISARLQELEGRVDQHIIPKE exon 10
tlvlyalgvvlqdntawplayr exon 11 X. laevis
FDPDRFNDETAKQSLTLLGFSGSQECPELR exon 12
FAYMVAMVLLSVLVRKLHLLPVKGQVMETKYELVTSPKEEAWITVSKRS exon 13
CYP20 Fugu rubripes (pufferfish)
No accession number
Scaffold_486
59% TO CYP20 human
MLDFAIFAVTFVIVLVGAVLYLYP (0)
SSRRASGIPGLNPTDEK (2)
11654 DGNLQDIVGRGSLHEFLVSLHQEFGPVASFWFGSRPVVSLGSLQQLRQHINPNHST 11487 (1)
DSFETMLKSLLGYHSGGGGASTDSIIRKKVYQGAIDTTLKNNFPLVLK (0)
LVDELVGKWKSFPEDQHTPLCAHQLVLAMKTITQLALGESFSEDARVIAFRKNHDV (0)
IWSEIGKGYMDGSLEKSTSRKGHYEK (1)
ALSEMESTLLSVVKERKSQRNKSVFVDSLIQSTLTERQ
IMEDCMVFMLAGCAITAN
VCIWALHFLSTSEEVQDRLYKEFEEVLGSSPVSLEKIPQLR
YCQQVLNETLRTAKLTPIAARLQEVEGKVDQHLIPKE
SLVIYALGVILQDSDTWNAPYR
FDPDRFEEESVKKSFHLLGFSGSQTCPELR
FAYTVATVLLSVLVRQLKLHRLKDTLMEVRSELVSTPRDETWITFNLRN*
CYP20 Tetraodon nigroviridis (freshwater pufferfish)
>FS_CONTIG_2529_2 Length = 13425 from http://fugu.hgmp.mrc.ac.uk/blast/
>FS_CONTIG_2529_1 Length = 1009 from http://fugu.hgmp.mrc.ac.uk/blast/
MLDFAIFAVTFVVILVGAVLYLYP
SSRRASGVPGLNPTDEK
XXXXXXXXXXXDIVARGSVQEFLVSLHQEFGPVASFWFGSRPVVSLGSLQQLQQHANPNRSS
DSFETMLKSLLGYHSGGGGASTENIIRKKVYQGAIDATLKNNFPLVLK
LVDELVGKWTSSPEDQHTPLCAHQLVLAMKSITQLALGESFSQDARVVSFRRNYDA
IWSEVGKGFMDGSLERSTSRKGRYEX
ALSEMEATLLSVVKDRKSQRKTSVLVDTLLQSTLTDRQ
IMEDCMVFTLAGCAITAN
VCIWALHFLSSYEDVQDRLHQELEEVLGSGSVSLEKIPQLR
YCQQVLNETVRTAKLTPVAAGLQEVEGKVDQHLIPKE
TLVIYALGVILQDSHTWDAPCR
FHPDRFEEESVRKSFRLLGFSGSQTCPELR
VAYTVATVLLSAVVRQLRLHRLEDTLVEVRSELVSTPREETWITFSRRN
CYP20 Danio rerio (zebrafish)
Assembled CYP20 from zebrafish ESTs
BQ259821 faa04d08.y1 zebrafish fin day3 regeneration
BM185037 fv16g05.x2 zebrafish adult brain
BG985721 2543 NICHD Zebrafish normalized I
BM070720 fu98e06.y1 zebrafish adult brain cDNA
CA472036 AGENCOURT_10739799 NCI_CGAP_ZKid1 cDNA (kidney?)
BI981894 fu52c12.y1 zebrafish adult brain Danio rerio cDNA
BM083017 fu28a11.y1 Campbell zebrafish ovary cDNA MLDFAIFAVTFVIILIGAVLYLYPSSRRASGVPGLNPTEEKDGNLQDIVNKGSLHEFLVG
LHDEFGSVASFWFGARPVVSLGAVNQLRQHINPNWTTDSFETMLKSLLGYQSGSGVGLTE
SMMRKKVYEGAINKTLENNFPLLLQQVEELVDKWASYPKSQHTPLCAHLLGLAMKAVTQL
AMGSRFRDDAEVIRFRKNHEAIWSQ
IGKGYLDGSLEKSSSRKAHYESALAEMESVLKSVAKQRPGQGSSQSFVNYLLQANLTER
QVMEDGMVFTLAGCVITANLCIWAVHFLSVSEAVQDRLYHELVEVLGDEPVSLEKIPQLR
YCQQVLNETVRTAKLTPVAARLQEVEGKVDQHIIPKETLVIYALGVVLQDADTWSLPYRF
NPDRFAEESVMKSFSLLGFSGSQACPELRFAYTVATVLL
STLVRRLRMHRVDGQVVEARYELVTTPKDDTWITVSKRN*
CYP20 Danio rerio (zebrafish)
>ctg10765 genomic contig CYP20 74% to fugu
9501 MLDFAIFAVTFVIILIGAVLYLYP (0) 9572
SSRRASGVPGLNPTEEK (2?)
DGNLQDIVNKGSLPEFLVGLH
DEFGSVASFWFGARPVVSLGAVNQLRQHINPNWT (1) 10024
12291 TDSFETMLKSLLGYQSGSGVGLTESMMRKKVYEGAINKTLENNFPLLLQ (0) 12439
12929 QVEELVDKWASYPKSQHTPLCAHFL 13003 frameshift
13003 GLAMKAVTQLAMGSRFRDDAEVIRFRKNHEA (0) 13095
15738 IWSEIGKGYLDGSLEKSSSRKAHYES (1?) 15815 AC boundary istead of AG
15897 ALAEMESVLKSVAKQRPGQGSSQSFVNYLLQANLTERQ (0) 16010
16583 VMEDGMVFTLAGCVITAN (1) 16636
17689 LCIWAVHFLSVSEAVQDRLYHELVEVLGDELVSLEKIPQLR (2) 17811
19293 YCQQVLNETVRTAKLTPVAARLQEVEGKVDQHVIPKE (0) 19403
21269 TLVIYALGVVLQDADTWSLPYR (2) 21334
21425 FNPDRFAEESVMKSFSLLGFSGSQACPELR (2) 21514
FAYTVATVLLSTLVRRLRMHRVDGQVVEARYELVTTPKDDTWITVSKRN* from wz10135.3
CYP20 Oryzias latipes (medaka fish)
BJ524824.1 MF01SSB cDNA Oryzias latipes cDNA
BJ003683.1 BJ003683 MF01SSA cDNA Oryzias latipes cDNA
MLDFAIFAVTFVVILVGAVLYLYPSSRRASGVPGLFPTDEKDGNLQDIVDRGSLHEFLV
GLHEQFGPVASFWFGRQPVVSLGSVDPLRQHINPNHTTDSFETMLKSLLGYQAGAGGGAN
ESVMRKKLYESAINNALKNSFPAVLKVAEELVDKWSSVPEDQHIPLCAHLLGLALKTV
TQLALGERFKDDAEVISFRKNHEAIWSEIGKGYMDGSLEKSSSRKRHYE SA
LSDMEATLLAV AKDRKAQRRQTA FVDALLQSGLTERQIMEDCMVFTLAGC
VITANLCIWALHFLSTAEDVQEKLCQEVEDLFGSDPVSLDRIPQLKYCQQVLNE
TVRTAKLTPVAARLXEVEGKVGQHVIPKETLVIYALG
VVLQDADTWSTPYRFDPDRFQDESARKSFCLLGFSGSQTCPELRFAYTVATVLLATLVRRLKLRPLK
About 26 aa missing at end
CYP20 Salmo salar (Atlantic salmon)
GenEMBL CA063128.1 ssalrgb509318 mixed_tissue Salmo salar cDNA.
GenEMBL CB516811.1 ssalrgb509318_rev mixed_tissue Salmo salar cDNA.
GenEMBL CB513409.1 ssalrgb531212_rev mixed_tissue Salmo salar cDNA.
BG935303.1 SL1-0624 Atlantic Salmon liver Salmo salar cDNA clone SL1-0624
MLDFAIFAVTFVIFLVGAVLYLYPSSRSASGIPGLNPTEEKDGNLQDIVNRGSLHEF
LASLHGQFGPVASFWFGGRPVVSLGSVDQLRQHINPNRTTDSFETMLKSLLGYQSGTGGG
ATEAVMRKKLYESAVNNTLEKNFPMLLKLVEELVGKWQSFPKDQHTPLCAHLLGLAMKAVTQ
18 amino acids missing here
RKNHEAIWSEIGKGYLDGSMEKSSIRKEHYESA
LAEMETVLMSVAKDRKGQRSQTAFVDTLLQSNLTERQVME
DSMVFTLAGCVITANLCIWAVHFLST
SEDVQEKLHQELEDVLGSEPVSLDKIPQLRYFQQVLNETVRTAKLTPIAARLQENEGKVD
QHIIPKETLVIYALGVVLQDADTWSCPYKFDPDRFTEDSARKSFSLLGFSGNQACPELRF
AYTVATVVLSTVVRQLKLYQVKGQVVEARSELVSTPKDDTWITVSRRS*
21A Subfamily
CYP21A1P human
GenEMBL M13935 (3206bp)
White,P.C., New,M.I. and Dupont,B.
Structure of human steroid 21-hydroxylase genes.
Proc. Natl. Acad. Sci. U.S.A. 83, 5111-5115 (1986)
97% to 21A2 NT_033167.1|Hs6_33343
329415 MLLLGLLLLLPLLAGARLLWNWWKLRSLHLLPLAPGFLHLLQPDLPIYLLGLTQKFGPIYRLHLGLQ 329215
329117 DVVVLNSKRTIEEAMVKKWADFAGRPEPLTCK 329022
LVSKNYPDLSL
328702 XXWSLLWKAHKKLTRSALLLGIRDSMEPVVEQLTQEFCE 328592
RMRAQPGTPVAIEEEFSLLTCSINCYLTFGDKIK 328383
328294 EDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLR 328193
328091 FFPNPGLRRLKQAIEKRDHNEEKQLRQHK
327835 ESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHLHMAAVDLLIGGTETTANTLSWAVV
327654 FLLHHPE 327634
IQQRL*EELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLWPVV
327292 PLALPHRTTRPS 327257
SISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWP 327069
326971 DRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPH
326791 CSVILKMQPFQVRLQPRGMGAHSPGQNQ 326708
CYP21A1P human with congenital adrenal hyperplasia
GenEMBL M26857 (4034bp) X05445
Rodrigues,N.R., Dunham,I., Yu,C.Y., Carroll,M.C.,
Porter,R.R. and Campbell,R.D.
Molecular characterization of the HLA-linked steroid
21-hydroxylase B gene from an individual with congenital
adrenal hyperplasia.
EMBO J. 6, 1653-1661 (1987)
CYP21A1P human
GenEMBL S60612 (426bp)
Collier,S., Tassabehji,M. and Strachan,T.
A de novo pathological point mutation at the 21-hydroxylase locus:
implications for gene conversion in the human genome.
Nature Genetics 3, 260-265 (1993)
CYP21A2 human
GenEMBL M21544 M21545 M21547 to M21550 M23224 M23225
M31022 M31023 (ten segments)
Globerman,H., Amor-Gueret,M., Parker,K.L., New,M.I. and White,P.C.
Nonsense mutation causing steroid 21-hydroxylase deficiency
J. Clin. Invest. 82, 139-144 (1988)
CYP21A2 human
GenEMBL M12792 M23280 (5141bp)
Higashi,Y., Tanae,A., Inoue,H., Hiromasa,T., and
Fujii-Kuriyama,Y.
Aberrant splicing and missense mutations cause steroid 21-
hydroxylase [P-450(C21)] deficiency in humans: possible gene
conversion products.
Proc. Natl. Acad.Sci USA 85, 7486-7490 (1988)
CYP21A2 human
GenEMBL X54940
Partanen,J. and Campbell,R.D.
Rapid characterisation of mutant P450c21 Genes by PCR
unpublished (1993)
CYP21A2 human
GenEMBL X58898 to X58908 PIR S26485 (97 amino acids)
PIR S26484 (371 amino acids) PIR S29670 (495 amino acids)
PIR S29671 (372 amino acids) PIR S26584 (371 amino acids)
PIR S29673 (372 amino acids)
Helmberg,A., Tabarelli,M., Dobler,G. and Kofler,R.
Identification of molecular defects causing congenital adrenal
hyperplasia by cloning of PCR-amplified 21-hydroxylase genes
unpublished (1992)
CYP21A2 human with congenital adrenal hyperplasia
GenEMBL M28548 (4044bp) X05449
Rodrigues,N.R., Dunham,I., Yu,C.Y., Carroll,M.C.,
Porter,R.R. and Campbell,R.D.
Molecular characterization of the HLA-linked steroid
21-hydroxylase B gene from an individual with congenital
adrenal hyperplasia.
EMBO J. 6, 1653-1661 (1987)
Note: mutant gene, 2 amino acid differences
CYP21A2 human with congenital adrenal hyperplasia
GenEMBL M26856 X05448 (4042bp)
Rodrigues,N.R., Dunham,I., Yu,C.Y., Carroll,M.C.,
Porter,R.R. and Campbell,R.D.
Molecular characterization of the HLA-linked steroid
21-hydroxylase B gene from an individual with congenital
adrenal hyperplasia.
EMBO J. 6, 1653-1661 (1987)
Note: normal gene
CYP21 rat
GenEMBL U56853(1964bp)
Zhou,M.Y., Vila,M.C., Gomez-Sanchez,E.P. and Gomez-Sanchez,C.E.
Cloning of two alternatively spliced 21-hydroxylase cDNAs in the
rat adrenal.
unpublished (1996)
MLLPGLLLLLLLLLLAGTRWLWGQWKLWKLRLPPLAPGFLHFLQPNLPVYLFGLAQKLGP
IYRIRLGLQDVVVLNSNKTIEEALIQKWVDFAGRPQILDGKMNFDLSMGDYSLTWKAHKK
LSRSALVLGMRDSMEPLVEQLTQEFCERMRAQAGASVAIHKEFSLLTCSIISCLTFGDKQ
DSTLLNATHSCVRDLLKAWNHWSVQILDIIPFLRFFPNPGLWKLKQFQESRDHIVMQELK
RHKDSLVAGQWKDMIDYMLQGVEKQRDARDPGQLHERHVHMSVVDLFVGGTETTAATLSW
AVAFLLHHPEIQKRLQEELDLKLAPSSQLLYKNRMQLPLLMATIAEVLRLRPVVPMALPH
RATKASSISGYDIPKDTIIIPNIQGANLDEMVWELPSKFWPDRFLESGKSPRIPTFGCGA
RVCLGEPLARLEFFVVLARLLQTFTLLPPPDGTLPSLQPLPYTGINLLIPPFQVRLQPRN
LAPQDQGQK
CYP21 rat
GenEMBL U56854
alternatively splice form
CYP21 pig
GenEMBL S53049 M83939 (4792bp) Swiss Q02390 (492 amino acids)
PIR S28169 (492 amino acids)
Burghelle-Mayeur,C., Geffrotin,C. and Vaiman,M.
Sequences of the swine 21-hydroxylase gene (Cyp21) and
a portion of the opposite-strand overlapping gene of unkown
function previously described in human.
Biochim. Biophys. Acta 1171, 153-161 (1992)
CYP21 sheep
PIR A43349 (497 amino acids) B43349 (479 amino acids)
Crawford, R.J., Hammond, V.E., Connell, J.M. and Coghlan, J.P.
The structure and activity of two cytochrome P450c21 proteins
encoded in the ovine adrenal cortex.
J. Biol. Chem. 267, 16212-16218 (1992)
B43349 is truncated early after alternate splicing
CYP21 Fugu rubripes (pufferfish)
No accession number
Scaffold_15
44% to CYP21 human over 454 amino acids
this is the first fish CYP21 sequence found
MCSFLFCSSFSAPPAVKRNLPLSLWQLPLRPSSPPIPGPPCRFLIGNMTE
28230 LMHDHLPIHLTNLAKRYGNIYRLKCGNTT 28144 (1)
28058 AMIVLNSSDIIREALVKKWSDFAGRAVSYT 27969 (1)
27888 ADIVSGGGRNISLGDYTEEWKALRRLVHGALQRCCKHSLHNVIERQALQLRK 27754 (0)
VLVDYRGGAVDLSEDFTVAASNVIITLVFGKE (0)
YDKSSSELQQLHRCLNEIVALWGSTWISALDTFPLLR (0)
KFPNPVFSRLLREVSRRDEIIRKHLNQFK (0)
CVLCCAQSEGHRRTDVITGSLLEG (0)
26896 VLTDMHVHMATVDLLIGGSETTAAWLNWTVAFLLHRPE (0)
FQTKVYEELCTVLEGRYPKYSDRQRLPILCSLIHEVLRLRPVAPLAVPHKAIRDS (2)
SIAGYFIPRNTIIIPNLFGAHHDPEVWSDPYSFKP (1)
26238 ERFLEGGGGSTRALIPFGGGARLCLGETVAKMELFLFTAYLLRDFCFVLPDSEAPLPDLR 26059
26058 GVASVVLKIKSFTVIARPRTGP* 25990
CYP21 Tetraodon nigroviridis (freshwater pufferfish)
GenEMBL AL281449.1 C0BG094DE12LP1 G Tetraodon nigroviridis
genomic clone 094J24 T7.Length = 895
AL233853.1 C0BG007BE04XD1 G Tetraodon nigroviridis
genomic clone 007I08 T7.Length = 1079
86% to Fugu CYP21
MGCIFFFFYLPFSAPPAVKRSLLQSLCGLLHRPSSPSIPGPPCRFLIGNMTE
LMQDHLPIHLTDLAKRYGNIYRLKCGNTS
AMVVLSSGDVIREALVKKWSDFAGRSVSYT
ADIVSGGGRTISLGDYTEEWKAHRRLVHSAL (frameshift) ERCXKQSLHDVIERQALQLRK
Missing exon 4 and part of exon 5
GSAWISALDTFPLLR
KFPNPVFSRLLREVTRRDEIIRKHLNQYK
CVLCCVQSQDNKSTDVITGSLLEG
VLTDVHVHMATVDLLIGGTETTAAWLNWTVAFLLHRPE
IQTKVYEELCTVLEGRYPKYSDRHRLPVLCSLVHEVLRLRPVAPLAVPHKAVRDS
SIAGYFIPKNTIIIPNLFGAHHDPXVWPDPYSFXX
Missing exon 11
22A Subfamily
CYP22A1 Caenorhabditis elegans (nematode worm)
GenEMBL U39648 (28581 bp) AF407572, NM_171699, NM_171698
cosmid T13C5.1
Jia,K., Albert,P.S. and Riddle,D.L.
DAF-9, a cytochrome P450 regulating C. elegans larval development
and adult longevity
Development 129 (1), 221-231 (2002)
daf-9 mutant This gene may be in the pathway to
synthesize a ligand for DAF-12, a nuclear receptor.
Daf-9 has a role in larval development.
DAF comes from abnormal DAuer Formation
23A Subfamily
CYP23A1 Caenorhabditis elegans (nematode worm)
GenEMBL U39472 (42282 bp)
see CYP14A1 for reference
cosmid B0304.3
24A Subfamily
CYP24 rat
GenEMBL L04608 to L04619, S52625 to S52636
Ohyama,Y., Noshiro,M., Eggertsen,G., Gotoh,O., Kato,Y.,
Bjorkhem,I. and Okuda,K.
Structural characterization of the gene encoding rat
25-hydroxyvitamin D3 24-hydroxylase.
Biochemistry 32, 76-82 (1993)
CYP24 rat
GenEMBL Z28351 (1419bp)
Hahn,C.N., Kerry,D.M., Omdahl,J.L. and May,B.K.
Identification of a vitamin D responsive element in the promoter of
the
gene for the rat 25-hydroxyvitamin D3 24-hydroxylase
Nuc. Acids Res. 22, 2410-2416 (1994)
CYP24 rat
GenEMBL X59506 (3209bp)
Ohyama,Y., Noshiro,M. and Okuda,K.
Cloning and expression of cDNA encoding 25-hydroxyvitamin D3 24-
hydroxylase.
FEBS Lett. 278, 195-198 (1991)
CYP24 rat
GenEMBL D17792 (?bp)
Ohyama, Y., Ozono,K., Uchida,M., Shinki,T., Kato,S., Suda,T.,
Yamamoto,O.,
Noshiro,M. and Kato,Y.
Identification of a vitamin D-responsive element in the 5'-flanking
region of the rat
25-hydroxyvitamin D3 24-hydroxylase gene.
J. Biol. Chem. 269, 10545-10550 (1994)
CYP24 rat
GenEMBL U03112 (?bp)
Zierold,C., Darwish,H.M. and DeLuca,H.F.
Identification of a vitamin D-response element in the rat calcidiol
(25-hydroxyvitamin D3) 24-hydroxylase gene.
Proc. Natl. Acad.Sci. USA 91, 900-902 (1994)
CYP24 human
GenEMBL S67623 (776bp)
Labuda,M., Lemieux,N., Tihy,F., Prinster,C. and Glorieux,F.H.
Human 25-hydroxyvitamin D 24-hydroxylase cytochrome P450
subunit maps to a different chromosomal location than that of
pseudovitamin D-deficient rickets.
J. Bone Miner. Res. 8, 1397-1406 (1993)
CYP24 human
GenEMBL L13286 (3254bp)
Chen,K.-S., Prahl,J.M. and DeLuca,H.F.
Isolation and expression of 1,25-dihydroxyvitamin D3
24-hydroxylase cDNA.
Proc. Natl. Acad. Sci. USA 90, 4543-4547 (1993)
CYP24 human
NM_000782
MSSPISKSRSLAAFLQQLRSPRQPPRLVTSTAYTSPQPREVPVC
PLTAGGETQNAAALPGPTSWPLLASLLQILWKGGLKKQHDTLVEYHKKYGKIFRMKLG
SFESVHLGSPCLLEALYRTESVPQRLEIKPWKAYRDYRKEGYGLLILEGEDWQRVRSA
FQKKLMKPGEVMKLDNKINEVLADFMGRIDELCDERGHVEDLYSELNKWSFESICLVL
YEKRFGLLQKNAGDEAVNFIMAIKTMMSTFGRMMVTPVELHKSLNTKVWQGHTLAWDT
IFKSVKACIDNRLEKYSQQPSADFLCDIYHQNRLSKKELYAAVTELQLAAVETTANSL
MWILYNLSRNPQVQQKLLKEIQSVLPENQRPREEDLRNMPYLKACLKESMRLTPGVPF
TTRTLDKATVLGEYALPKGTVLMLNTQVLGSSEDNFEDSSQFRPERWLQEKEKINPFA
HLPFGVGKRMCIGRRLAELQLHLALCWIVRKYDIQATDNEPVEMLHSGTLVPSRELPI
AFCQR
CYP24 Fugu rubripes (pufferfish)
No accession number
Scaffold_4128
MLWRLRGALTLPPELTVLDAIPGPTNWPLVGSLFELLRKGGLTRQHEAL
VDYHKKFGKIFRLKLGSFESVHIGAPCLLESLYRTEGSYPQRLEIKPWTAYRDMRDEAYGLLIL
EGKDWQRVRRAFQQKLMKPTEVVKLDRKINE
VLEDFVSRIGKTNIGGKIEDLYFELNKWSFES
ICLVLYDKRFGLLQDKVNEEAMNFITAVKT
MMSTFGLMMVTPVELHKSLNTKTWQDHTAAWDRIFST
AKVYIDKKLKRNSVIAPDDLIGDILHQSRLSKKELYAAITELQIGGVET
8608 TANSMLWAIFNLSRNPGAQRRLLEEIRTVVPPEQDPCGEHIKSMPYLKACLKESMR 8441 (2)
7830 ISPSVPFTSRTLDKDTVLGDYAIPKG 7753
TVLMINSHALGSSEDYFDDGKKFKPERWLREHGTINPFAH
VPFGIGKRMCIGRRLAELQMSLFLQLVRDFE
IVATDNEPLDVIHSGLLVPNRELPVAFIKR
25A Subfamily
CYP25A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z66495 ( 40145bp)
see CYP14A1 for reference
cosmid C36A4.1
CYP25A2 Caenorhabditis elegans (nematode worm)
GenEMBL Z66495 ( 40145bp)
see CYP14A1 for reference
cosmid C36A4.2
CYP25A3 Caenorhabditis elegans (nematode worm)
GenEMBL Z66495 ( 40145bp)
see CYP14A1 for reference
cosmid C36A4.3
CYP25A4 Caenorhabditis elegans (nematode worm)
GenEMBL Z66495 ( 40145bp)
see CYP14A1 for reference
cosmid C36A4.6
CYP25A5 Caenorhabditis elegans (nematode worm)
GenEMBL AF038613
see CYP14A1 for reference
F42A6
CYP25A6P Caenorhabditis elegans (nematode worm)
GenEMBL U50072
K06B9.1
missing C-terminal
26A Subfamily
Cyp26a1 mouse
No accession number
Jim Ray
submitted to nomenclature committee
Note: new family in mammals, homolog to human ESTs R51129 and R21282
Cyp26a1 mouse
No accession number
Martin Petkovich
submitted to nomenclature committee
Note: new family in mammals, homolog to human ESTs R51129 and R21282
Cyp26a1 mouse
GenEMBL Y12657
Fujii, H., Sato, T., Kaneko, S., Gotoh, O., Fujii-Kiriyama, Y.,
Osawa, K.,
Kato, S. and Hamada, H.
Metabolic inactivation of retinoic acid by a novel P450
differentially expressed in
developing mouse embryos.
EMBO J. 16, 4163-4173 (1997)
Note: new family in mammals, homolog to human ESTs R51129 and R21282
CYP26A1 human
GenEMBL NM_000783
White,J.A., Beckett-Jones,B., Guo,Y.D., Dilworth,F.J., Bonasoro,J.,
Jones,G. and Petkovich,M.
cDNA cloning of human retinoic acid-metabolizing enzyme (hP450RAI)
identifies a novel family of cytochromes P450
J. Biol. Chem. 272 (30), 18538-18541 (1997)
Note: new family in mammals, equal to human ESTs R51129 and R21282
CYP26A1 human
NM_000783
MGLPALLASALCTFVLPLLLFLAAIKLWDLYCVSGRDRSCALPL
PPGTMGFPFFGETLQMVLQRRKFLQMKRRKYGFIYKTHLFGRPTVRVMGADNVRRILL
GDDRLVSVHWPASVRTILGSGCLSNLHDSSHKQRKKVIMRAFSREALECYVPVITEEV
GSSLEQWLSCGERGLLVYPEVKRLMFRIAMRILLGCEPQLAGDGDSEQQLVEAFEEMT
RNLFSLPIDVPFSGLYRGMKARNLIHARIEQNIRAKICGLRASEAGQGCKDALQLLIE
HSWERGERLDMQALKQSSTELLFGGHETTASAATSLITYLGLYPHVLQKVREELKSKG
LLCKSNQDNKLDMEILEQLKYIGCVIKETLRLNPPVPGGFRVALKTFELNGYQIPKGW
NVIYSICDTHDVAEIFTNKEEFNPDRFMLPHPEDASRFSFIPFGGGLRSCVGKEFAKI
LLKIFTVELARHCDWQLLNGPPTMKTSPTVYPVDNLPARFTHFHGEI
CYP26A1 zebra fish
GenEMBL U68234
White, J.A., Guo, Y.-D., Baetz, K., Beckett-Jones, B., Bonasoro, J.
Hsu, K.E., Dilworth, F.E., Jones, G. and Petkovich, M.
Identification of the retinoic acid-inducible all trans retinoic
acid 4-hydroxylase.
J. Biol. Chem. 271, 29922-29927 (1996)
Note: new family in vertebrates, homolog to human ESTs R51129 and
R21282
CYP26A1 Fugu rubripes (pufferfish)
No accession number
Scaffold_12575
67% to 26A1 human
missing C-term zebrafish seq shown in lower case
2144 MAVSALLATFLCTIVLPLLLFLVTVKLWEVY (frameshift)
VIRERDSACP (frameshift)
SPLPPG (frameshift)
TMGLPFIGETLQLILQ (0)
1689 RRKFLRMKRQKYGYIYRTHLFGNPTVRVTGANNVRHILLGEHRLVAV 1552
1551 QWPASVRTILGSDTLSNVHGAQHKTKKK 1465 (0)
1230 AIMQAFSREALEFYIPAMQHEVQAAVQEWLAKDSCVLVY
PEMKRLMFRIAMQILLGFQLE 1051 (frameshift)
1049 QIKTDEQKLVEAFEEMIKNLFSLPIDMPFSGLYR 948 (0)
784 GLKARNFIHAKIEENIKRKLRESNSDSKCRDALQQLIDSSKKSGQVLSMQ 635 (0)
548 VLKESATELLFGGHETTASTATSLIMFLGLNPEVLDKLRHELSDKVMHKGF 396 (1)
329 LDLRSLNLETLEQLKYTSCVIKETLRMNPPVPGGFRVALKTFELG 195 (0)
100 GYQIPKGWNVIYSICDTHDVAEIFP 26 (frameshift)
24 NKEDFQPE 1 end of Scaffold_12575
RFMMKNCGDSSRFQYIPFGGG
srmcvgkefakvllkiflveltqhcnwilsngpptmktgptiypvdnlptkftsyvrn
CYP26A1 Xenopus laevis
GenEMBL AF057566
Hollemann,T., Chen,Y., Grunz,H. and Pieler,T.
Regionalized metabolic activity establishes boundaries of retinoic
acid signalling.
EMBO J. 17, 7361-7372 (1998)
CYP26B1 human
GenEMBL AC007002
Nelson, D.R. A second CYP26 P450 in humans and zebrafish: CYP26B1
Archives of Biochemistry and Biophysics 371, 345-347 (1999)
MLFEGLDLVSALATLAACLVSVTLLLAVSQQLWQLRWAATRDKSCKLPIPKGSMGFPLIGETGHWLLQ
GSGFQSSRREKYGNVFKTHLLGRPLIRVTGAENVRKILMGEHHLVSTEWPRSTRMLLGPNTVSNS
IGDIHRNKRKVFSKIFSHEALESYLPKIQLVIQDTLRAWSSHPEAINVYQEAQ
KLTFRMAIRVLLGFSIPEEDLGHLFEVYQQFVDNVFSLPVDLPFSGYRR
GIQARQILQKGLEKAIREKLQCTQGKDYLDALDLLIESSKEHGKEMTMQELKDGTLELIF
AAYATTASASTSLIMQLLKHPTVLEKLRDELRAHGILHSGGCPCEGTLRLDTLSGLRYLD
CVIKEVMRLFTPISGGYRTVLQTFELDGFQIPKGWSVMYSIRDTHDTAPVFKDVNVFDP
DRFSQARSEDKDGRFHYLPFGGGVRTCLGKHLAKLFLKVLAVELASTSRFELATRTFPRI
TLVPVLHPVDGLSVKFFGLDSNQNEILPETEAMLSATV
CYP26B1 Fugu rubripes (pufferfish)
No accession number
Scaffold_4267
74% to 26B1 human
8448 MLFDSFDLVSALATLAACLVSMALLLAVSQQLWQLRWTATRDRNCKLPMPKGSMGFPFIGETCHWLLQ 8651
17930 GSGFHASRRQKYGNVFKTHLLGRPLIRVTGAENIRKVLMGEHTLVTVDWPQSTSTLLGPNSLA 18118
18119 NSIGDIHRKKRK 18154
1487 VFAKVFSHEALESYLPKIQQVIQESLRVWSSNPEPINVYR 1606 (2)
1689 ESQRLSFTMAVRVLLGFRVSEEEMKHLFSTFQDFVDNLFSLPIDLPFSGYRK 1844 (0)
1921 GIRARDTLQKSIEKAIREKPLCSQGKDYSDALDVLMESAKENGSELTMQELK 2076 (0) exon 4
2851 ESTIELIFAAFATTASASTSLIMQLLRHPPVLERLREELRARGL exon 5,6 fused
LHNGCLCPEGELRLDTIVSLKYLDCVIKEVLRLFTPVSGAYRTAMQTFELD 3135 (0) exon 5,6 fused
3271 GVQIPKGWSVMYSIRDTHDTSTVFKDVDVFDPDRFSQERGEDKEGRFHYLPFGGGVRSCLGKQLA exon 7
TLFLRILAIELASTSRFELATRQFPRVITVPVVHPVDGLKVKFYGLDSNQNEIMAKSEELLGAAV* 3663 exon 7
CYP26C1 human
GenEMBL AL358613.11 May 2, 2001
522 amino acids, 6 exons, (0) = phase 0 intron
52% to 26B1 human, also 15 amino acid insertion in exon 5 vs. 26B1
MFPWGLSCLSVLGAAGTALLCAGLLLSLAQHLWTLRWMLSRDRASTLPLPKGSMGWPFFGETLHWLVQ (0)
GSRFHSSRRERYGTVFKTHLLGRPVIRVSGAENVRTILLGEHRLVRSQWPQSAHILLGSHTLLGAVGEPHRRRRK (0)
VLARVFSRAALERYVPRLQGALRHEVRSWCAAGGPVSVYDASKALTFRMAARILLGLRL
DEAQCATLARTFEQLVENLFSLPLDVPFSGLRK (0)
GIRARDQLHRHLEGAISEKLHEDKAAEPGDALDLIIHSARELGHEPSMQELK (0)
ESAVELLFAAFFTTASASTSLVLLLLQHPAAIAKIREELVAQGLGRACGCAPGAAGGSEGPPPD
CGCEPDLSLAALGRLRYVDCVVKEVLRLLPPVSGGYRTALRTFELD (0)
GYQIPKGWSVMYSIRDTHETAAVYRSPPEGFDPERFGAAREDSRGASSRLHYIPFGGGARSCLG
QELAQAVLQLLAVELVRTARWELATPAFPAMQTVPIVHPVDGLRLFFHPLTPSVAGNGLCL*
CYP26C1 mouse
GenEMBL AC110212.1
84% to 26C1 human exon 5
ELAVELLFAAFFTTASASTSLILLLLQHPAAITKIQQELSAQGLGRACTCTPRASGSPPDCGCEPDLSLAMLGRLRYVDCVVKEVLRLLPPVSGGYRTALRTFELD
CYP26C1 Fugu rubripes (pufferfish)
No accession number
Scaffold_11741
Equally similar to 26B1 and 26C1 human
But C-terminal is 68% to 26C1 while 58% to 26B1
Lower case region very poor match may not be correct exon structure here.
6362 MLGLVSALATALTTLLLLLLLLALTRQLWSFRWSLTRDRRCELPLPKGSMGWPLVGETFQWLFQ 6171 (0)
5571 GSNFHISRRKRHGNVFKTHLLGKPLVRVTGAENIRKILLGEHSLVCTQWPQSTRIILGPN 5392
5391 ALVNSIGELHKRKRK 5347 (0)
4963 ILAKVFSRKALESYLPRLQEVIKCEIAKWCAEPGSVDVYAATRSLTFRIAIGVLLGLHL 4781
4780 EEERIDYLAQIFGQLMSNLFSLPIDAPFSGLRK (0)
3972 GIKARKILHANMEKIIEKKMERQQEEEEYRDAFDYMLSTSKEQGQQISIQELK 3814 (0)
3581 ETAVELIFAAHSTTASAATSLVLQLLHHPEVVERVRVELEAQKLcynslnlpsqa 3417 (1)
3377 ctfpqsqchasnLSLDKLNQLHYIDCVIKEVLRFLPPVSGGYRTALQTFELD 3222 (0)
2770 GYQIPKGWTVMYSIRDTHETAEIFQNPELFDPDRFVTAQVESRSSRFSYVPFGGGVR 2600
2599 SCVGKELAQIILKTLTIELIRTCKWTLATEKFPKMQTVPIVHPVNGLHVNFMYKNLHEIDH* 2414
27A Subfamily
CYP27A1 human
Swiss Q02318 (531 amino acids)
Cali J.J., Russell D.W.
Characterization of the human sterol-27-hydroxylase: A mitochondrial
P450
that catalyzes multiple oxidation reactions in bile acid
biosynthesis.
J. Biol. Chem. 266, 7774-7778 (1991)
CYP27A1 human
GenEMBL X59812 (2107bp)
Guo,Y., Strugnell,S., Back,D.W. and Jones,G.
Transfected human liver cytochrome P-450 hydroxylates vitamin D
analogs at different side-chain positions
Proc. Natl. Acad. Sci. U.S.A. 90, 8668-8672 (1993)
CYP27A1 human
NM_000784
MAALGCARLRWALRGAGRGLCPHGARAKAAIPAALPSDKATGAP
GAGPGVRRRQRSLEEIPRLGQLRFFFQLFVQGYALQLHQLQVLYKAKYGPMWMSYLGP
QMHVNLASAPLLEQVMRQEGKYPVRNDMELWKEHRDQHDLTYGPFTTEGHHWYQLRQA
LNQRLLKPAEAALYTDAFNEVIDDFMTRLDQLRAESASGNQVSDMAQLFYYFALEAIC
YILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWN
AIFSFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASGQLSPREAMGSLPELLM
AGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKE
TLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQPHRWL
RNSQPATPRIQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGEL
KSVARIVLVPNKKVGLQFLQRQC
CYP27A1 rat
GenEMBL M73231 (2300bp)
Shayiq,R.M. and Avadhani,N.G.
Sequence complementarity between the 5'-terminal regions of mRNAs
for
rat mitochondrial cytochrome P-450c27/25 and a growth hormone-
inducible
serine protease inhibitor: a possible gene overlap.
J. Biol. Chem. 267, 2421-2428 (1992)
CYP27A1 rat
GenEMBL U17363, U17369 to U17376 genomic sequence
Mullick, J., Addya,S., Sucharov,C. and Avadhani,N.G.
Localization of a transcription promoter within the second exon of
the cytochrome P-450c27/25 gene for the expression of the major
species of two-kilobase mRNA.
Biochemistry 34, 13729-13742 (1995)
CYP27A1 rabbit
PIR A90152 (21 amino acids)
Dahlbaeck, H.
Characterization of the liver mitochondrial cytochrome P-450
catalyzing the 26-hydroxylation of 5beta-cholestane-3alpha,
7alpha,12alpha-triol.
Biochem. Biophys. Res. Commun. 157, 30-36 (1988)
PIR A90155 (21 amino acids)
Dahlbaeck, H.
Biochem. Biophys. Res. Commun. (1989) 159:370
CYP27A1 pig
no accession number
Kjell Wikvall
77% identical to human
CYP27A1 Fugu rubripes (pufferfish)
No accession number
Scaffold_3437
46% to 27A1 missing N-terminal exon upstream of scaffold
136 (0) ILEKGRYGPIYRNGMNAVSVSTAKLLGEVLRNDDKFPNRGDMSIWKEYRDLRGYGYGPFTE 321 (2)
536 KDERWYNLRAVLNKRMLRPKDALQYGDTIGEVVTDFIRRIYFLRQRSPTGDVVTDLNNELYHFSLE 733 (1)
816 AIASILFETRLGCLEEEIPTGTQDFINAISQMFSNNFQVFLMPKWSRGVLPYWRRYVAGWDGIFSF 1013 (1)
1206 ATRLIDRKMEFIQQHLDNNQNVEGEYLTYLLSNTQMSIKDVYGSVSELLLAGVDT 1370 (0)
1465 TSNTLTWTLHLLSKYPQCQEILFKEVSTSVPADRAPSAEEVTRMPYLRAVVKESLR 1632 (2)
1756 MFPVIPMNGRILADKDVMIGGYQFSKN 1836 (0)
1949 TAFNFSHYAIGRDEDTFPEPATFMPERWLQDSHNRPNAFGAIAFGFGVRGCVGRRIAELEMYSFLCH 2149 (0)
2308 LMRHFEIKPDPKMGELKSVCRTVLIPDKPVSLRFLDRGSGHAA* 2439
CYP27A2 Fugu rubripes (pufferfish)
No accession number
Scaffold_697
53% to 27A1 mouse
29906 MASFTALRCAAIGARNSALRPATLPSRNLNLQATSEAANLKGIADLPGPNTYKILYWLFVKGYGERSHLLQ 30118 (0)
30735 GKLKNIYGPMWRWKLGPYDFVSVASPELIARVIQQEGRYPVRVQLPHWKEYRDLRGQAYGLHVE 30926 (2)
31027 TGPEWSRLRSALKPRMLKLREVVALSPDESR 31119 frameshift EVDGDLL
29 aa of this exon missing in region of poor seq and a small sequence gap
31334 GISAILFETRLGCLGEKVDPNVQRFISGVNDMLSLSDITYLFPRWTRSFVPVWKRFAQAWDDISDV 31531 (1)
31614 ASSLIDRRIAEIDARVANGQSVEGLYLTYLLSSDKMSRAEISTCITDLLLGGVDT 31778 (0)
32763 TSNTLSWALYHLAKDPVAQDRLYDEVNSVCPNHHQPTTDDLANMPFLKAVIKEVLR 32930 (2)
33004 LYPVVHQNARFISENDVILNDYWFPKK (0)
TQFHLCHYSVCHDETQFKHAERFLPERWLRHSAPLSGYYQHHPYSFIPFGVGVRACVGKRVAELEMXXXXXX 33348
this exon runs into a sequence gap
YFALTR (0) this is a Fugu seq for the end of the upper exon that is no
longer in the databases. The last exon is missing
CYP27A3 Fugu rubripes (pufferfish)
No accession number
Scaffold_6002 Length = 16767
= LGS139924.x1 57% to 27A1 I-helix
= LGS125183.x1 Cyp27a1
First 16 aa and 49-81 supported by EST from AU050037 Paralichthys olivaceus
aa 49 on also supported by AW343479 zebrafish EST
MFRNRLLTVGLRASVPHREGLHRTAVNYAGARRRHASSAATEITEHNVR
QKTMEDLGGPSFLTTLNWLFLKGYLPKTQQMQ (0)
4619 VEHSKIYGPLWKSKYGPMVVVNVASADLIEQVLRQEGRHPVRTDMPHWRRYRALRNQAHGPLTE 4428
exons 3 and 4 in a sequence gap
1891 AEQMVQKKMEEIQNKVDLHQDVEGAYLTHLLLSEKMTVTEILGSITELLLAGVDT 1715
1661 TSNTISWALYQLAQNPSIQDQLYHEVRSVCPGNKMPDSDDIAQMPYLKAVIRETLR
LYPVVPGNARVTVDKEIVVGGYLFPKQ (0)
754 TLFHLCHYCVSHDENIFPNSRVFQPERWLRGREEKSKQHPFGSVPFGFGVRACLGRRV 581
580 AELEMYLLLSRVRAVQTGLTAAGEGNQEVL*
>CYP27A fragment a Fugu rubripes (pufferfish)
No accession number
a may be exon 4 of 27A3
LPC42076.x1 39% to 27A1 202-276 79% to LPC42075.x1 gene duplication? Exon 4
GISSVLFESRMGCLNDEVPEETQKFIYSVGEMFRLSAVVVLFPQSVWPYLPLWKKFVAAWDYLFKV
>CYP27A fragment b Fugu rubripes (pufferfish)
No accession number
may be exon 4 of 27A3
LPC42075.x1 35% to 27A1 79% to LPC42076.x1 gene duplication? Exon 4
GISSVLFESRLGCLNDEVPEETRRVIYSVGEMCRLSAVVVLFPQSGWPYLPVWARLGAAGDYLLQF
27B Subfamily
CYP27B1 rat
GenEMBL AF000139
St-Arnaud,R., Messerlian,S., Moir,J.M., Omdahl,J.L. and
Glorieux,F.H.
The 25-hydroxyvitamin D 1-alpha-hydroxylase gene maps to the
pseudovitamin D-deficiency rickets (PDDR) disease locus
J. Bone Miner. Res. 12, 1552-1559 (1997)
previously named CYP40. This sequence has errors in it.
CYP27B1 rat
GenEMBL AB001992
Cloning and expression of rat 25-hydroxyvitamin D3-1-alpha-
hydroxylase cDNA .
Shinki, T., Shimada, H., Wakino, S., Anazawa, H., Hayashi, M.,
Saruta, T., DeLuca, H.F. and Suda, T.
Proc. Natl. Acad. Sci. USA 94, 12920-12925 (1997)
Note: this sequence has been called CYP27B1 in this paper. The name
CYP40 was
given in May 1997 based on the sequence from John Omdahl that was
not
completely accurate at the time of submission for a name. It was
necessary to
revise the name CYP40 to CYP27B1.
CYP27B1 mouse
GenEMBL AB006034
Takeyama, K-i., Kitanaka, S., Sato, T., Kobori, M., Yanagisawa, J. and
Kato, S.
25-hydroxyvitamin D3 1 alpha hydroxylase and vitamin D synthesis.
Science 227, 1827-1830 (1997)
CYP27B1 human
GenEMBL AB005989 cDNA sequence
Takeyama,K., Kitanaka,S., Sato,T., Kobori,M., Yanagisawa,J. and
Kato,S.
25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis
Science 277, 1827-1830 (1997)
CYP27B1 human
GenEMBL AB005990 gene sequence
Murayama,A., Kitanaka,S., Takeyama,K. and Kato,S.
Human 25-hydroxyvitamin D3 1alpha-hydroxylase gene
Unpublished (1997)
CYP27B1 human
GenEMBL AB005038 cDNA sequence AB006987 gene sequence
Monkawa,T., Yoshida,T., Wakino,S., Shinki,T., Anazawa,H.,
Deluca,H.F., Suda,T., Hayashi,M. and Saruta,T.
Molecular cloning of cDNA and genomic DNA for human
25-hydroxyvitamin D3 1alpha-hydroxylase
Biochem. Biophys. Res. Commun. 239, 527-533 (1997)
CYP27B1 human
GenEMBL AF020192 cDNA sequence
Fu,G.K., Lin,D., Zhang,M.Y., Bikle,D.D., Shackleton,C.H.,
Miller,W.L. and Portale,A.A.
Cloning of human 25-hydroxyvitamin D-1alpha-hydroxylase and
mutations causing vitamin D-dependent rickets type 1
Mol. Endocrinol. 11, 1961-1970 (1997)
CYP27B1 human
GenEMBL AF0027152 gene sequence
Fu,G.K., Portale,A.A. and Miller,W.L.
Complete structure of the human gene for the vitamin D 1alpha-
hydroxylase,
P450c1alpha.
DNA Cell Biol. 16, 1499-1507 (1997)
CYP27B1 human
NM_000785
MTQTLKYASRVFHRVRWAPELGASLGYREYHSARRSLADIPGPS
TPSFLAELFCKGGLSRLHELQVQGAAHFGPVWLASFGTVRTVYVAAPALVEELLRQEG
PRPERCSFSPWTEHRRCRQRACGLLTAEGEEWQRLRSLLAPLLLRPQAAARYAGTLNN
VVCDLVRRLRRQRGRGTGPPALVRDVAGEFYKFGLEGIAAVLLGSRLGCLEAQVPPDT
ETFIRAVGSVFVSTLLTMAMPHWLRHLVPGPWGRLCRDWDQMFAFAQRHVERREAEAA
MRNGGQPEKDLESGAHLTHFLFREELPAQSILGNVTELLLAGVDTVSNTLSWALYELS
RHPEVQTALHSEITAALSPGSSAYPSATVLSQLPLLKAVVKEVLRLYPVVPGNSRVPD
KDIHVGDYIIPKNTLVTLCHYATSRDPAQFPEPNSFRPARWLGEGPTPHPFASLPFGF
GKRSCMGRRLAELELQMALAQ
ILTHFEVQPEPGAAPVRPKTRTVLVPERSINLQFLDR
CYP27B1 Fugu rubripes (pufferfish)
No accession number
Scaffold_470
52% to 27B1 human
MLQQALRVSCRSASPLVKWMERWAECASARPQAVKPLGDMPGPSVASFAWDLFAKRGLSRLHELQ (0)
LEGVRRYGPMWKASFGPILTVHVADPALIEQVLRKEGQHPMRSDLSSWKDYRRLRGHHYGLLTS (2)
51430 EGEEWQSIRSLLGKHMLRPKAVEAYDQTLNSVVDDLITKLRLRRSSQGLVTDIASEFYRFGLE 51630 (1)
51726 GVSSVLFESRIGCLDKIVPEETERFIQCINTMFVMTLLTMAMPSWMHQLFPKPWNVFCQCWDYMFDF (1)
AKGHIDQRMAAEAEKIARGEEVEGRYLTYFLSRTSLPMKTVYSNVTELLLAGVDT (0)
52280 ISSTLSWSLYELSRHQAVQASLREEVLSVLGGRRVPTAADVAQMPLLKATIKEVLR 52444 (2)
52527 LYPVIPANARVITERDIQVGGYLIPKN 52610 (0)
52697 TLITLCHYATSRDPAVFPRPDEFLPQRWLNKEQSHHPYASVPFGVGKRSCIGRRIAELELYLAVAR 52894 (0)
53237 ILLEFDIKPDPEGISVKPMTRTLLVPENVINLQFTER* 53347
CYP27C1 human
GenEMBL AC027142
BM562765 BI459427 ESTs
43% identical to 27A1 assembled gene
intron starting with QIH ending in VDT is from Celera's data
CRA_Gene|hCG42613 /len=10487. This Celera sequence is still missing the C-terminal. Probable last exon is now found in AC027142. AG Intron boundary is in the same location as CYP26B1. Stop codon is one codon away from 26B1s stop codon. Length is preserved from cys to intron. (n) = intron phase, 9 exons
1 85452 MQTSAMALLARILRAGLRPAPERGGLLGGGAPRRPQPAGARLPAGARAEDKGAGRPGSPPG 85634 61
62 85635 GGRAEGPRSLAAMPGPRTLANLAEFFCRDGFSRIHEIQ (0) 85748 99
100 39574 QKHTREYGKIFKSHFGPQFVVSIADRDMVAQVLRAEGAAPQRANMESWREYRDLRGRATGLISA (2) 39371 163
164 43984 EGEQWLKMRSVLRQRILKPKDVAIYSGEVNQVIADLIKRIYLLRSQAEDGETVTNVNDLFFKYSME (1) 43787 229
230 41743 GVATILYESRLGCLENSIPQLTVEYIEALELMFSMFKTSMYAGAIPRWLRPFIPKPWREFC 41564 290
291 41563 RSWDGLFKFS 41534 300 (1)
301 QIHVDNKLRDIQYQMDRGRRVSGGLLTYLFLSQALTLQEIYANVTEMLLAGVDT (0) 354 (Celera sequence)
355 110201 TSFTLSWTVYLLARHPEVQQTVYREIVKNLGERHVPTAADVPKVPLVRALLKETLR (2) 110034 410
411 108566 LFPVLPGNGRVTQEDLVIGGYLIPKG (0) 108489 436
437 108006 TQLALCHYATSYQDENFPRAKEFRPERWLRKGDLDRVDNFGSIPFGHGVRSCIGRRIAELEIHLVVIQ (0) 107794 504
505 102503 LLQHFEIKTSSQTNAVHAKTHGLLTPGGPIHVRFVNRK* 102619 542
CYP27C1 Fugu rubripes (pufferfish)
No accession number
Scaffold_1410
75% to 27C1 human
MSVMNKLTTTCWTNFYGDRRNKQMLFVLRCLHKSATSGTFGVAREEPLPERLITSTDATKKRLPKT
19233 LAEMPGPGTISNLFEFFWRDGFSRIHEIQ 19319 (0)
20076 IEHSKMYGKIFKSRFGPQLVVSVADRDLVAEVLRAE
GVAPQRANMESWHEYRDMRRRSTGLISA 20261 (2)
EGEDWLRMRSVLRQLIMRPRDVAVFSDDVSEVVDEVVDDLIKR
IVCLRSQSSDGTTICNINDLFFKYAME (1)
20741 GIAAILYECRLGCLSQKIPQETEDYIDALHLMFSSFKTTMYAGAIPKWLRPV 20911
20912 FPKPWEEFCDSWDGLFRF 20968 (1)
SVHVDKRLKQIESQLQRGEKVTGGLLTYMLVAKEMSVEEIYANVTEMLLAGvdt (0)
23004 TSFTLSWASYLLARHPDVQQQIHAEVMRVLGSEKVATAEDVQHLPFIRGLVKETLR 23183 (2)
LFPVLPGNGRITQDDMVLGGYFIPKG
23623 TQLALCHYSTSLDDENFPSSLEFRPDRWIRKHSSDRLDNFGSIPFGYGIRSCIGKRI 23793
23794 AELEMHLALIR (0)
23998 IIQKFHVCVSPLTTDVKAKTHGLLCPGAPINLQFIDREI* 24117
CYP27C1 Silurana tropicalis (frog)
GenEMBL BQ392731 AL629634 AL595312
74% to human 27C1 not counting the divergent N-terminal
87% to Xenopus 27C1
MAALGQLLRGSARLEGLARSFHRFPGAQAAGQALEHEQAEGVLGATVKGSPMVKN
LKEMPGPSTMANLVEFFWRDGFGRIQEIQQKHARQYGRIFKSHFGPQFVVSIADKDMVAQ
VLRAERDAPQRANMESWHEYRELRGRSTGLISAEGEKWLNMRSVLRQKILRPRDVAMYSG
GVNEVVEDLVKRIRKLRVQESDGLTVTNVNDLYFKYSMEAIATILYECRLGCLDDQIPQQ
TKEYIEALELMFSMFKTTMYAGAIPKWLRPLIPKPWREF
CRSWDGLFKFSQIHVDDRLRQIESQLEKGEEVQGGVLTHLLLSKELDLEEIYA
NMTEMLLAGVDTTSFTLSWATYLLAKNPGIQEAVYQQIVQNFGKDQVPTAEDVPKMPLVRAVVKETL
RLFPVLPGNGRVTQDDLVVGGYFIPKGTQLALCHYSTSYDAECFPAAEEFRPERWIRSGN
LERKENFGSIPFGYGIRSCIGRRVAELEMHLLLIQLLQ
NLEIKPSPQTTTVLPKTHGLLCPGGKINVRFVDRQ*
CYP27C1 Xenopus laevis (African clawed frog)
GenEMBL BJ086834 CA982419 BQ385474 BJ069739
87% to Silurana 27C1
MAVLGHLLKGSARLEGLARGFHQFPKIQAAGQALEQEQAEGEL
GARAKEAPMM KSLKDMPGPSTLANLVEFFWRDGFGRIHEIQQKHTRQYGRIFKSHFGPQF
VVSIADKDLVAQVIRAERDAPQRANMESWHEYRELRGRSTGLISAEGEKWLNMRSVLRQK
ILRPRDVAMYTGGVNEVIGDLVKKIHKLRAQESDGLTVTNVNDLYFKYSMEAIATVLYEC
RLGCLDDIIPQQTKEYIAALELMFSMFKTTMYAGGLPKWLRHHNFPNPWKEF
53aa gap see Silurana seq
HMTEMLLAGVDTTSFTLSWATYLLAKNPSIQESVYQQIVQNLGKDQVPTAEDVTKIPMVR
AVVKETLRLFPVLPGNGRVTQDDLVLDGYLIPKGTQLALCHYSTSYDDKCFPGAEEFKPE
RWIRSGYLERKENFGSIPFGNGIRSCIGRRVAELEIHLLLIQL
LQNFEIKLSPETMTVLPKTHGLLCPGEKINVRFLDRQ*
CYP27C1 Gallus gallus (chicken)
GenEMBL BU235126 BU209688
Partial seq missing N and C-terminals
78% to Silurana 27C1 82% to human 27C1
PGLSGSQSLEVRSGAENKAARPGELLEPSPQLGRV KSLHEMPGPNTLYNLYEFFWKDGFGRIHE
IQQKHTQEYGKIFKSHFGPQFVVSIADRDMVAQVLRSEGRAPQRANMESWQEYRDLRGR
ATGLISAEGEQWLKMRSVLRQKILKPKDVAVYSGGVNEVITDLIKRIYTLRSQEEDGETV
TNVNNLFFKYSMEGVATILYECRLGCLENNVPQQTVEYIEALELMFSMFKTTMYAGAIPR
WLRPFIPKPWREFCRSWDGLFKFSQIHVDNKLKSIQS
QLDQGEEVNGGLLTYLLVSKELTLEEIYA
NMTEMLLAGVDTTSFTLSWAIYMLAKHPEVQQRVYEEIINKLGKDQAPVNRDVPKLPLIR
AVLKETLR
CYP27C1 mouse and rat
Mouse is missing this sequence in blast searches. In humans it lies between
BIN1 and ERCC3 on chr 2. This region does not contain 27C1 in mouse (chr 18)
unless there is a sequence gap. Blast of the rat genome
(Rat Genome Sequencing Consortium Assembly Posted date: Dec 4, 2002)
also had no hits, so 27C1 may have been lost in rodent evolution.
It is present in human, Fugu, Xenopus, Silurana (another frog) and chicken.
The absence of 27C1 may be a useful synapomorphy for comparing rodents to other mammals.
28A Subfamily
CYP28A1 Drosophila mettleri
GenEMBL U89746 (1895bp)
Danielson,P.B., MacIntyre,R.J. and Fogleman,J.C.
Molecular cloning of a family of xenobiotic-inducible drosophilid
cytochrome p450s: evidence for involvement in host-plant
allelochemical resistance.
Proc. Natl. Acad. Sci. U.S.A. 94, 10797-10802 (1997)
note: 369mt, full length, one of 109 seqs submitted to Nomenclature
committee
CYP28A2 Drosophila mettleri
GenEMBL U89747 (1764bp)
Danielson,P.B., MacIntyre,R.J. and Fogleman,J.C.
Molecular cloning of a family of xenobiotic-inducible drosophilid
cytochrome p450s: evidence for involvement in host-plant
allelochemical resistance.
Proc. Natl. Acad. Sci. U.S.A. 94, 10797-10802 (1997)
note: 43mt, full length, one of 109 seqs submitted to Nomenclature
committee
CYP28A3 Drosophila nigrospiracula (a desert dwelling Drosophilid)
GenEMBL U91565 (451bp)
Danielson,P.B., MacIntyre,R.J. and Fogleman,J.C.
Molecular cloning of a family of xenobiotic-inducible drosophilid
cytochrome p450s: evidence for involvement in host-plant
allelochemical resistance.
Proc. Natl. Acad. Sci. U.S.A. 94, 10797-10802 (1997)
54 amino acids
CYP28A4 Drosophila hydei (a desert dwelling Drosophilid)
GenEMBL U91566 (366bp)
Danielson,P.B., MacIntyre,R.J. and Fogleman,J.C.
Molecular cloning of a family of xenobiotic-inducible drosophilid
cytochrome p450s: evidence for involvement in host-plant
allelochemical resistance.
Proc. Natl. Acad. Sci. U.S.A. 94, 10797-10802 (1997)
54 amino acids
Cyp28a5 Drosophila melanogaster
GenEMBL AC018242 6065-8082 also AC001660
CYP28B1 Musca domestica (housefly)
no accession number
Nannan Liu
44% identical to 28A1 over 498 amino acids
submitted to nomenclature committee 7/29/99
Cyp28c1 Drosophila melanogaster
GenEMBL AC014191 comp(8254-10002) also AL133495
Cyp28d1 Drosophila melanogaster
GenEMBL AC017780 comp(28385-30262) also AC009355 AC008324 AC008327
Cyp28d2 Drosophila melanogaster
GenEMBL AC017780 comp(31545-33456) AC008324
29A Subfamily
CYP29A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z69787 and AL022276 (Y102F5)
C44C10.2 (with modifications)
note: GeneFinder translation is incorrect in some regions.
First exon probably ends at 11181 coding for 35 amino acids as does
the first exon
of CYP29A2.
There is probably a sequence error in the stop codon at 11155-11157.
2nd exon probably runs from 11227-11293.
11400-11518 is the third exon.
12465 to 12979 should be one exon.
There is probably a sequence error in the stop codon at 12879-12881.
some segments around I-helix missed by genefinder. N-terminal of
genefinder translation is probably a different gene. Matches
F23A7.3, but this gene
does not have a P450 sequence downstream.
CYP29A2 C. elegans
GenEMBL Z74043
T19B10.1
nearly the whole gene of wEST00713, CEMSH91R, missing 5' end
CYP29A2 C. elegans
GenEMBL Z74040
T19B10.1
5' end of gene from Z74043
CYP29A3 C. elegans
no accession number
Y108G3 contig 541
CYP29A4 C. elegans
GenEMBL Z99102
B0331.1
30A Subfamily
CYP30 Mercenaria mercenaria (northern quahog, a clam)
GenEMBL AF014795 (1628bp)
Brown, D., Clark,G.C. and Van Beneden,R.J.
A novel cytochrome P450 from the clam, Mercenaria mercenaria
Unpublished
31A Subfamily
CYP31A1P C. elegans
GenEMBL Z68213
C01F6.3
missing 25 amino acids at C-helix plus in frame stop codon at ERK*
CYP31A2 C. elegans
GenEMBL Z68336 and Z92789
F22B3 and H02I12
This gene is definitely different than CYP31A3, there are 8 amino
acids differences
and the introns are divergent.
CYP31A3 C. elegans
no accession number
T16C6 this sequence revised according to Y17G9.contig 61
cosmid T16C6 lies 3' of E04A4 and 5' of R11E3. Y17G9 contig 61 covers
this
region. E04A4 ends at 29981 of Y17G9 contig 61 R11E3 starts at 49037
of Y17G9
contig 61 this sequence lies between 41545-45037
CYP31A4P C. elegans
no accession number
Pseudogene related to CYP31A sequences 44849-45028 of Y17G9.contig61
C-TERMINAL exon fragment, This sequence is inside the last intron of
CYP31A3
32A Subfamily
CYP32 C. elegans
GenEMBL U53148
C26F1.2
33A Subfamily
CYP33A1 C. elegans
GenEMBL U55365
C12D5.7
33B Subfamily
CYP33B1 C. elegans
GenEMBL U50311
C25E10.2
33C Subfamily
CYP33C1 Caenorhabditis elegans (nematode worm)
GenEMBL AF039053
C45H4.a near 35k
CYP33C2 Caenorhabditis elegans (nematode worm)
GenEMBL AF039053
C45H4.b near 38k
also on Y10C10 contig 60. Probable end of 33C2 gene is on contig 57,
but the intron ends of these two contigs do not overlap yet.
CYP33C3 Caenorhabditis elegans (nematode worm)
GenEMBL AF016676
F41B5.b 5k to 7k
CYP33C3 Caenorhabditis elegans (nematode worm)
GenEMBL D35162
EST 5 prime read with heme signature
3 prime fragment of this clone yk18a10 = D32508 (not in coding region)
this is a portion of clone YK824
CYP33C4 Caenorhabditis elegans (nematode worm)
GenEMBL AF016438
F44C8 whole gene of EST CEL10E1
CYP33C4 Caenorhabditis elegans
GenEMBL M88882 (501bp)
EST CEL10E1
2nd frame contains PPGP and KKYG from N-terminal region of
many P450s.
CYP33C5 Caenorhabditis elegans (nematode worm)
GenEMBL AF016676
F41B5.c
CYP33C6 Caenorhabditis elegans (nematode worm)
GenEMBL AF016676
F41B5.e
CYP33C7 Caenorhabditis elegans (nematode worm)
GenEMBL AF016676
F41B5.d
CYP33C8 Caenorhabditis elegans (nematode worm)
GenEMBL AF003385
R08F11
CYP33C9 Caenorhabditis elegans (nematode worm)
GenEMBL AF016449
C50H11
CYP33C10P Caenorhabditis elegans (nematode worm)
GenEMBL AF016676
F41B5.a
contains first two exons split by 3700bp and no C-terminal sequence.
CYP33C11 Caenorhabditis elegans (nematode worm)
no accession number
Y49C4 contig 103
33D Subfamily
CYP33D1 Caenorhabditis elegans (nematode worm)
GenEMBL Z92804
K05D4.4 (previously found on F10A3 and F11A5 at early stages of
sequencing)
CYP33D2X Caenorhabditis elegans (nematode worm)
GenEMBL Z92830
F11A5
this sequence is really the same gene as CYP33D1
CYP33D3 Caenorhabditis elegans (nematode worm)
GenEMBL Z81487 (C54E10), AL021470 (Y17D7A.4) and Z98877 (Y69H2)
GenEMBL AL020988 (Y80D3)
33E Subfamily
CYP33E1 Caenorhabditis elegans (nematode worm)
GenEMBL U61945
C49C8.4
CYP33E2 Caenorhabditis elegans (nematode worm)
GenEMBL U61952
F42A9.5
CYP33E3P Caenorhabditis elegans (nematode worm)
GenEMBL U61952
F42A9.4
missing C-terminal 3' neighbor is C49C8 (with CYP33E1)
34A Subfamily
CYP34A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z81119 and AL022301 (Y75B12)
T10H4.10
There are two P450s listed in this clone as one gene
This is the first one
CYP34A2 Caenorhabditis elegans (nematode worm)
GenEMBL Z81119 and AL022301 (Y75B12)
T10H4.11
There are two P450s listed in this clone as one gene
This is the second one
CYP34A3 Caenorhabditis elegans (nematode worm)
GenEMBL Z81047 and AL022301 (Y75B12)
C41G6.1
CYP34A4 Caenorhabditis elegans (nematode worm)
GenEMBL AF068712
T09H2.1
CYP34A5 Caenorhabditis elegans (nematode worm)
GenEMBL AF039050
B0213.10
CYP34A6 Caenorhabditis elegans (nematode worm)
GenEMBL AF039050
B0213.11
CYP34A7 Caenorhabditis elegans (nematode worm)
GenEMBL AF039050
B0213.12
CYP34A8 Caenorhabditis elegans (nematode worm)
GenEMBL AF039050
B0213.14
CYP34A9 Caenorhabditis elegans (nematode worm)
GenEMBL AF039050
B0213.15
CYP34A10 Caenorhabditis elegans (nematode worm)
GenEMBL AF039050
B0213.16
35A Subfamily
CYP35A1 Caenorhabditis elegans (nematode worm)
GenEMBL U97008
C03G6 near 19k
CYP35A2 Caenorhabditis elegans (nematode worm)
GenEMBL U97008
C03G6 near 22k
CYP35A3 Caenorhabditis elegans (nematode worm)
no accession number
K09D9
CYP35A4 Caenorhabditis elegans (nematode worm)
GenEMBL AF016418
C49G7.8
CYP35A5 Caenorhabditis elegans (nematode worm)
GenEMBL AF039049
K07C6.5 [and part of F40C5 contig 6 9-578 plus strand Note:
newest version of
F40C5 does not contain this P450. probably an error corrected by the
sequencers]
NOTE: K07C6 IS NOW IN GENBANK AND IT CONTAINS 4 P450S
It is part of a contig with T09H2 and B0213 that has 13 P450s in a
cluster
6 P450s on B0213 appear to have inserted themselves into a cluster
of
three olfactory receptor-like genes. K09D9 is the next clone 3' and
it has at least
one more P450. C49G7 is next with one more P450
Contig order K07C6 T09H2 B0213 K09D9 C49G7
35B Subfamily
CYP35B1 Caenorhabditis elegans (nematode worm)
GenEMBL AF039049
K07C6.4, [F40C5 contigs 5 and 14 Note: newest version of F40C5 does
not
contain this P450. probably an error corrected by the sequencers]
CYP35B2 Caenorhabditis elegans (nematode worm)
GenEMBL AF039049
K07C6.3 [and F40C5 contig 15 Note: newest version of F40C5 does not
contain
this P450. probably an error corrected by the sequencers]
CYP35B3 Caenorhabditis elegans (nematode worm)
GenEMBL AF039049
K07C6.2
35C Subfamily
CYP35C1 Caenorhabditis elegans (nematode worm)
GenEMBL Z77652
C06B3.3
35D Subfamily
CYP35D1 Caenorhabditis elegans (nematode worm)
GenEMBL Z83105
F14H3 near 20k
CYP35D2P Caenorhabditis elegans (nematode worm)
GenEMBL Z83105
F14H3 near 14k
only N-terminal is present
36A Subfamily
CYP36A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z83220
C34B7.3
37A Subfamily
CYP37A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z81493 (F01D5) and Z92851 (Y39G8)
37B Subfamily
CYP37B1 Caenorhabditis elegans (nematode worm)
GenEMBL Z93381 and Z93389
F28G4 and T13F3
CYP38A1 Suberite domuncula (sponge)
GenEMBL Y17816 (1789bp)
Mueller,W.E.G., Wiens,M., Batel,R., Steffen,R., Borojevic,R. and
Custodio,M.R.
Establishment of a primary cell culture from a sponge: Primmorphs
from Suberites domuncula
Mar. Ecol. Prog. Ser. In press
most similar to the CYP4 family
MLDFVIFAITAVAGLIGILLFFYFSRSTETKPVSSASPTSTIPR
WSAPPADIEKGDLDVMMKKHGSLHQFLLHLHDNGKTPVTSFWWGKTHVVSFCSPQAFK
ESAVFVNRPVELFVGFEPLITPFSIQYANDEDWVQRSKCLYHTLKGDDLKSYFHHFVQ
IAQEEESLWSSYTSDKEVSLTKEVFPMTIKGIARTCFGDIFKDENELSKMAESYHVCW
RTMEEGVPEAGSKRETEFLKHRRVLEDIIRRIIQERKEGEDLQELPFIDSMLQNYDSE
DKIIADAISFMVGGFHTSGYMFTWMLWYLSSHPESQDRLRTEIERETGGERGDRLKEY
SLRADTFLRQVQDETIRLSTLAPWAARYSDKKVTVCGYTIPAKTPMIHALGVGLKNKT
VWENTDSWDPDRFSPNGRRGNDFCPFGVHSRRKCPGYLFSYFEVGVFASILLSRFEIV
PVEGQTVIQVHGLVTEPKDDIKIYIRSRKED"
CYP39 human
GenEMBL EST R07010 R11279 and UNIGENE entry Hs.25121
covers the C-terminal part of a P450. The 2 ESTs with coding
regions
are not found in UNIGENE, but the opposite end of EST R11279 =
R11221 and it
is in UNIGENE with 13 EST sequences all from the 3 prime noncoding
region.
This sequence is most like CYP4A11, but the percent identity is only
39%. Since this is the most conserved region of P450s, the sequence
must be in a
new family. More sequence is known form the mouse homolog.
ENLLLIKWCVLETIRLKAPGVITRKVVKPVEILNYIIPSGDLLMLSPFWLHRNPKYFPEPELFKPERWEKGKFRRKHSFL
GTASWA
FGAGSSQCPGKVFALLEVQVC
CYP39A1 human
AC008104 AL035670 note heme region exon corrected 1/18/02
MELISPTVIIILGCLALFLLLQRKNLRRPPCIKGWIPWIGVGFEFGKAPLEFIEKARIK
YGPIFTVFAMGNRMTFVTEEEGINVFLKSKKVDFELAVQNIVYRT
ASIPKNVFLALHEKLYIMLKGKMGTVNLHQFTGQLTEELHEQLENLGTHGTMDLNNLVR
HLLYPVTVNMLFNKSLFSTNKKKIKEFHQYFQVYDEDFEYGSQLPECLLR
NWSKSKKWFLELFEKNIPDIKACKSAKDNSM
TLLQATLDIVETETSKENSPNYGLLLLWASLSNAVP
VAFWTLAYVLSHPDIHKAIMEGISSVFGKAG
KDKIKVSEDDLENLLLIKWCVLETIRLKAPGVITRKVVKPVEIL
NYIIPSGDLLMLSPFWLHRNPKYFPEPELFKPERW
KKANLEKHSFLDCFMAFGSGKFQCPARW
FALLEVQMCIILILYKYDCSLLDPLPKQ
SYLHLVGVPQPEGQCRIEYKQRI
CYP39 mouse
GenEMBL ESTs AA096922, AA606237
Note: this sequence has a poorly conserved I Helix and may not require
molecular
oxygen. The substrate may be a peroxide or some substance with its
own oxygen.
consensus
QFKTYDEGFEYGSQLPEWLLRNWSKSKRWLLALFEKNIGNIKAHGSAGHS
GTLLQAILEVVETETRQYSPNYGLVVLWAALANAPPIAFWTLGYILSHPDIHRTVL
ESISSVFGTAGKDKIKVSEDDLKKLLIIKWCILESVRLRAPGVITRKVVK
PVKILNHTVPSGDLLMLSPFWLHRNPKYFPEPESFKPERWKEANLDKYIF
LDYFMAFGGRKFQCPGKWFALLEIQLCIILVLYKYECSLLDPL
40A Subfamily
CYP40X rat, mouse, human (name changed to CYP27B1)
Note: This family was created based on the rat sequence AF000139 submitted to
the Nomenclature Committee in May 1997. This was the only sequence available at
the time and it was about 38% identical to rat CYP27. The sequence was
considered a new family and named CYP40. Since then, there have been additional
sequences determined for the rat mouse and human homologs. The rat sequence
AB001992 differs from the AF000139 sequence at 34 amino acids, mostly in two
regions that are probably frameshifts. The sequence AB001992 is 42.3% identical
to the rat CYP27A sequence. The mouse and human sequences are also more than 40%
identical to the CYP27A sequence. Therefore, they belong in the CYP27 family as
a new subfamily CYP27B1. Since there only appears to be one gene in each
species, all three species orthologs will be called CYP27B1. CYP40 has been
retired and this is indicated by the X in CYP40X.
41A Subfamily
CYP41 Boophilus microplus (southern cattle tick)
GenEMBL U92732 (414bp)
Crampton,A.L., Miller,C., Baxter,G.D. and Barker,S.C.
Expressed sequenced tags and new genes from the cattle tick,
Boophilus microplus.
Exp. Appl. Acarol. 22 (3), 177-186 (1998)
EST is from the middle of the P450
whole sequence known but still confidential.
42A Subfamily
CYP42 C. elegans
GenEMBL AL020988 (Y80D3), M89401 (EST cm08B12)
43A Subfamily
CYP43 C. elegans
GenEMBL AF026203
E03E2.1
44A Subfamily
CYP44 C. elegans
GenEMBL U21321
CELZK177
only mitochodrial-like P450 in C. elegans (missing part of I-helix
may be
pseudogene)
45A Subfamily
CYP45 Homarus americanus (American lobster)
GenEMBL AF065892 (1581bp)
Snyder,M.J.
Identification of a new cytochrome P450 family, CYP45, from the
lobster, Homarus americanus, and expression following hormone and
xenobiotic exposures
Arch. Biochem. Biophys. (1998) In press
46A Subfamily
CYP46 human
GenEMBL NM_006668
Lund EG, Guileyardo JM and Russell DW.
cDNA cloning of cholesterol 24-hydroxylase, a mediator of
cholesterol homeostasis in the brain.
Proc. Natl. Acad. Sci. U.S.A. 96, 7238-7243 (1999)
32% identity with Drosophila 4D2
ESTs H06539, H51951, R36281
mouse homolog EST AA096922
MSPGLLLLGSAVLLAFGLCCTFVHRARSRYEHIPGPPRPS
FLLGHLPCFWKKDEVGGRVLQDVFLDW
AKKYGPVVRVNVFHKTSVIVTSPESVK
KFLMSTKYNKDSKMYRALQTVFGER
LFGQGLVSECNYERWHKQRRVIDLAFSRSSLVSLMETFNEKAEQLVEILEAKADGQTPVSMQDMLTYTAMDILAK
AAFGMETSMLLGAQKPLSQAVKLMLEGITASRNTLAK
FLPGKRKQLREVRESIRFLRQVGRDWVQRRREALKRGEEVPADILTQILK
AEEGAQDDEGLLDNFVTFFIA
GHETSANHLAFTVMELSRQPEIVAR
LQAEVDEVIGSKRYLDFEDLGRLQYLSQ
VLKESLRLYPPAWGTFRLLEEETLIDGVRVPGNTPLL
FSTYVMGRMDTYFEDPLTFNPDRFGPGAPK
PRFTYFPFSLGHRSCIGQQFAQ
MEVKVVMAKLLQRLEFRLVPGQRFGLQEQATLKPLDPVLCTLRPRGWQPAPPPPPC
CYP46 Fugu rubripes (pufferfish)
No accession number
Scaffold_4537
60% to 46 human
MGVFNLIFGWISQASIFLLLLLFIALLGYCMYIKYTHMKYDHIPGPPRDS (2)
FFSGHSSKLLDIMKDDGVVHDMFLKW (2)
AETYGPVYKIYFLHHVMVFVSCPETTK (0)
EMLMSPKYTKDKFLHNRIGSLFGQR (2)
FLGNGLVTVRDHEKWYKQRRIMDPAFSSL (2)
YLRSLMGNFNETADKLMDKLSEIADNKTTANMLHLVNCVTMEV