P450s that have appeared since the 1993 P450 nomenclature update.

      This is Part B of the P450 bibiographic files.  
      Sequences covered are CYP3 to CYP9.

      This list now includes all Drosophila P450s

      This includes references that were incomplete and duplications
      of sequences that were already in the update.  If a sequence 
      is assigned an accession number that was not in the old update
      it is included in this list.  
      Added 4A23-4A25, 6X1 9J1, 9J2, 
      4F19 N-term (Jan 2002 added Fugu 3As and minipig 3A29v5)
      Added 6P2, 6P3, 6Y1, 6Z1, 9K1 added 6Z2, 6AA1, 6P4 and 9L1
      Added 4C31, 4C32, 4C33, 4C34, 4AN1, 4AN2, 4AN3, 4AP1, 4AP2

      Added all human genes and pseudogenes
      Added CYP8B1 pig
      Added 6AB3, 6AE1, 9A6 and 9A7, 4G19
      Added 25 Aedes and 104 Anopheles sequences
      Added CYP9G2
      This list was last revised on April 7, 2003. 
      Added Cyp3a58-ps to CYP3A62, wallaby 4B1, 4B3
      July 2, 2003 added 4F42, 3A64, 4T6, 4T7
      Last revised Aug 19 2003, added 9A12
      Last revised Sept 26, 2003 added accessions for some old seqs.

3A Subfamily

4A Subfamily

4B Subfamily

4C Subfamily

4D Subfamily

4E Subfamily

4F Subfamily

4G Subfamily

4H Subfamily

4J Subfamily

4K Subfamily

4L Subfamily

4M Subfamily

4N Subfamily

4P Subfamily

4Q Subfamily

4R Subfamily

4S Subfamily

4T Subfamily

5A Subfamily

6A Subfamily

6B Subfamily

6C Subfamily

6D Subfamily

6E Subfamily

6F Subfamily

7A Subfamily

7B Subfamily

8A Subfamily

8B Subfamily

9A Subfamily

9B Subfamily

9C Subfamily


3A Subfamily

CYP3A1      rat
            GenEMBL D13912 (2023bp)
            Kirita,S. and Matsubara,T.
            cDNA cloning and characterization of a novel member of steroid-
            induced cytochrome P450 3A in rats
            Arch. Biochem. Biophys. 307, 253-258 (1993)
            Note: only 11 differences and two missing amino acids with 
            3A1.  All changes are limited to a 113 amino
            acid region, probably represents a gene conversion.

CYP3A1      rat
            PIR S30378 (25 amino acids)
            Cooper, K.O., Reik, L.M., Jayyosi, Z., Bandiera, S., Kelley,
            M., Ryan, D.E., Daniel, R., McCluskey, S.A., Levin, W. and
            Thomas, P.E.
            Regulation of two members of the steroid-inducible cytochrome
            P450 subfamily (3A) in rats.
            Arch. Biochem. Biophys. 301, 345-354 (1993)

CYP3A1      rat
            GenEMBL L24207 (2015bp)
            Miyata,M., Nagata,K., Yamazoe,Y. and Kato,R.
            Nucleotide and deduced amino acid sequences of a new rat CYP3A form.
            Unpublished (1993)

CYP3A1      rat
            GenEMBL D29967 (2026bp) PIR S39797 (502 amino acids)
            Kirita,S. and Matsubara,T.
            cDNA cloning and characterization of a novel member of
            steroid-induced cytochrome P450 3A1 in rats.
            Arch. Biochem. Biophys. 307, 253-258 (1993)

CYP3A1      rat
            GenEMBL M28452 (897bp)
            Wrighton,S.A., Schuetz,E.G., Watkins,P.B., Maurel,P., Barwick,J.,
            Bailey,B.S., Hartle,H.T., Young,B. and Guzelian,P.
            Demonstration in multiple species of inducible hepatic cytochromes
            P-450 and their mRNAs related to the glucocorticoid-inducible
            cytochrome P-450 of the rat
            Mol. Pharmacol. 28, 312-321 (1985)

            Komori,M. and Oda,Y.
            PIR JX0334 (502 amino acids)
            A major glucocorticoid-inducible P450 in rat liver is not 3A1.
            J. Biochem. 116, 114-120 (1994)
            Note 12 amino acid diferences and 1 two amino acid deletion vs 3A1

CYP3A1      rat
            PIR S36137 (30 amino acids)
            Ribeiro, V. and Lechner, M.C.
            Cloning and characterization of a novel CYP3A1 allelic
            variant: Analysis of CYP3A1 and CYP3A2
            sex-hormone-dependent expression reveals that the CYP3A2
            gene is regulated by testosterone.
            Arch. Biochem. Biophys. 293, 147-152 (1992)
            This sequence agrees exactly with PIR JX0334, a 3A1 variant

CYP3A1      rat
            PIR S27107 (24 amino acids)
            PIR S21697 (504 amino acids)
            Telhada,M.B., Pereira,T.M. and Lechner,M.C.
            Effect of dexamethasone and phenobarbital on run-on transcription
            rate and CYP3A mRNA concentration in rat liver: changes during
            development
            Arch. Biochem. Biophys. 298, 715-725 (1992)

CYP3A2      rat
            GenEMBL M74443 (4230bp) U09725 to U09734 13 exons 
            Miyata,M., Nagata,K., Yamazoe,Y. and Kato,R.
            A gene structure of testosterone 6-beta-hydroxylase (P450IIIA)
            Biochem. Biophys. Res. Commun. 177, 68-73 (1991)
            U09725-U09734 report corrections to M74443

CYP3A2      rat
            Gen EMBL X79319 (8014bp)
            Miyata,M., Nagata,K., Shimada,M., Yamazoe,Y. and Kato,R.
            Structure of a gene and cDNA of a major constitutive form of       
            testosterone 6-beta-hydroxylase (P450/6-beta-A) encoding CYP3A2
            unpublished (1994)

CYP3A2      rat
            GenEMBL X79320 (1936bp) PIR S46938 (504 amino acids)
            Miyata,M., Nagata,K., Shimada,M., Yamazoe,Y. and Kato,R.
            Structure of a gene and cDNA of a major constitutive form of
            testosterone 6-beta-hydroxylase (P450/6-beta-A) encoding CYP3A2
            unpublished (1994)

CYP3A2      rat
            GenEMBL U09742 (1936bp)
            Miyata,M., Nagata,K., Shimada,M., Yamazoe,Y. and Kato,R.
            Structure of a gene and cDNA of a major constitutive form of
            testosterone 6-beta-hydroxylase (P450/6-beta-A) encoding CYP3A2: 
            Comparison of the cDNA with P450PCN2.
            unpublished (1994)

CYP3A2      rat
            Yamazoe,Y. et al. 
            Transcriptional elements directing a liver-specific expression of   
            P450/6bA(CYP3A2) gene encoding testosterone 6b-hydroxylase.
            Arch Biochem Biophys. 318, 71-79 (1995)

CYP3A2      rat
            PIR S30379 (25 amino acids)
            Cooper, K.O., Reik, L.M., Jayyosi, Z., Bandiera, S., Kelley,
            M., Ryan, D.E., Daniel, R., McCluskey, S.A., Levin, W. and
            Thomas, P.E.
            Regulation of two members of the steroid-inducible cytochrome
            P450 subfamily (3A) in rats.
            Arch. Biochem. Biophys. 301, 345-354 (1993)

CYP3A2      rat
            GenEMBL S45634 (1572bp)
            PIR S27108 (36 amino acids)
            Telhada,M.B., Pereira,T.M. and Lechner,M.C.
            Effect of dexamethasone and phenobarbital on run-on transcription
            rate and CYP3A mRNA concentration in rat liver: changes during
            development
            Arch. Biochem. Biophys. 298, 715-725 (1992)

CYP3A3      human
            PIR A25450 (504 amino acids) PIR A29410 (504 amino acids)
            Molowa, D.T., Schuetz, E.G., Wrighton, S.A., Watkins, P.B.,
            Kremers, P., Mendez-Picon, G., Parker, G.A. and Guzelian, P.S.
            Complete cDNA sequence of a cytochrome P-450 inducible by
            glucocorticoids in human liver.
            Proc. Natl. Acad. Sci. U.S.A. 83, 5311-5315 (1986)

CYP3A4      human
            GenEMBL D11131 (1345bp)
            Hashimoto,H. Toide,K., Kitamura,R., Fujita,M., Tagawa,S.,
            Kamataki,T. and Itoh, S.
            Gene structure of CYP3A4 an adult-specific form of cytochrome
            P450 in human 
            livers and its transcriptional control.
            Eur. J. Biochem. 218, 585-595 (1993)

CYP3A4      human
            GenEMBL J04449 (2849bp)
            Bork,P., Muto,T., Beaune,P.H., Srivastava,P.K., Lloyd,R.S. and 
            Guengerich,F.P.
            Characterization of mRNA species related to human liver cytochrome 
            P-450 nifedipine oxidase and the regulation of catalytic activity.
            J. Biol. Chem. 264, 910-919 (1989)
            nf-10 nifedipine oxidase (IIIA3)

CYP3A5P1    human
            GenEMBL L26985 (1599bp)
            Schuetz,J.D.
            unpublished (1994)

CYP3A5P2    human
            GenEMBL X90579 (?bp)
            Maurel,P. and Jounaidi,Y.
            unpublished (1995)
            note: a second pseudogene similar to CYP3A5P1

CYP3A5      human
            PIR A60558 (29 amino acids)
            Wrighton, S.A., Ring, B.J., Watkins, P.B. and Vandenbranden, M.
            Identification of a polymorphically expressed member of the
            human cytochrome P-450III family.
            Mol. Pharmacol. 36, 97-105 (1989)

CYP3A5      human
            GenEMBL S74699 (1526bp) S74700 (1667bp)
            Jounaidi,Y., Guzelian,P.S., Maurel,P. and Vilarem,M.J.
            Sequence of the 5'-flanking region of CYP3A5: comparative analysis
            with CYP3A4 and CYP3A7.
            Biochem. Biophys. Res. Commun. 205, 1741-1747 (1994)

CYP3A5      human
            GenEMBL AC005020 genomic sequence 13 exons
            Same intron exon structure as CYP3A43 from human
NT_033964.1|Hs7_34119 Homo sapiens chromosome 7 
alt exon 1 and 2 upstream of 3A5
239409 MDLIPNLAVETWLLLAVSLVLLYL + ex 1
243605 YGAHSHGLFKKLGIPGPTPLLFLGTTLSYHQ 243697 + ex2

CYP3A7      human
            NM_000765
MDLIPNLAVETWLLLAVSLILLYL
YGTRTHGLFKKLGIPGPTPL
PFLGNALSFRKGYWTFDMECYKKYRKVWGIYDCQQPMLAITDPDMIKTVLVKECYSVF
TNRRPFGPVGFMKNAISIAEDEEWKRIRSLLSPTFTSGKLKEMVPIIAQYGDVLVRNL
RREAETGKPVTLKHVFGAYSMDVITSTSFGVSIDSLNNPQDPFVENTKKLLRFNPLDP
FVLSIKVFPFLTPILEALNITVFPRKVISFLTKSVKQIKEGRLKETQKHRVDFLQLMI
DSQNSKDSETHKALSDLELMAQSIIFIFAGYETTSSVLSFIIYELATHPDVQQKVQKE
IDTVLPNKAPPTYDTVLQLEYLDMVVNETLRLFPVAMRLERVCKKDVEINGMFIPKGV
VVMIPSYVLHHDPKYWTEPEKFLPERFSKKNKDNIDPYIYTPFGSGPRNCIGMRFALV
NMKLALVRVLQNFSFKPCKETQIPLKLRFGGLLLTEKPIVLKAESRDETVSGA

NT_033964.1|Hs7_34119 chromosome 7 alt exon 1 and 2 upstream of 3A7
186958 xxLIPNLAVETWLLLAVSLVLHYL + ex 1
191087 VSLCRYETHSHGLFKNLGIPGPRPLLFLGTTLSYHQ 191194 + ex 2

CYP3A8      Macaca fasicularis (monkey)
            GenEMBL S53047 (2032bp) Swiss P33268 (503 amino acids)
            PIR S28168 (503 amino acids)
            Komori,M., Kikuchi,O., Sakuma,T., Funaki,J., Kitada,M.
            and Kamataki,T.
            Molecular cloning of monkey liver cytochrome P-450 cDNAs:
            similarity of the primary sequences to human cytochromes P-450.
            Biochim. Biophys. Acta 1171, 141-146 (1992)

CYP3A8      Macaca fasicularis (monkey)
            Swiss P25231 (22 amino acids)
            Ohta,K., Kitada,M., Hashizume,T., Komori M., Ohi,H., and   
            Kamataki,T.
            Biochim. Biophys. Acta 996, 142-145 (1989)

CYP3A8      Macaca fasicularis (monkey)
            PIR S36875 (13 amino acids)
            Ohmori, S., Horie, T., Guengerich, F.P., Kiuchi, M.and Kitada,M.
            Purification and characterization of two forms of hepatic microsomal
            cytochrome P450 from untreated cynomolgus monkeys.
            Arch. Biochem. Biophys. 305, 405-413 (1993)

CYP3A9      rat
            GenEMBL U46118(2101bp)
            Wang,H., Kawashima,H. and Strobel,H.W.
            cDNA cloning of a novel CYP3A from rat brain
            Biochem. Biophys. Res. Commun. 221 (1), 157-162 (1996)

CYP3A9      rat
            GenEMBL U60085(1882bp)
            Nef,P.
            Hepatic expression and inducibilty of CYP3A9 and other members of
            the rat CYP3A subfamily.
            unpublished (1996)

CYP3A10     hamster
            GenEMBL M73992 (2015bp)
            Miyata,M., Nagata, K., Yamazoe,Y. and Kato,R.
            Nucleotide and deduced amino acid sequence of a new rat CYP3A form
            unpublished (1993)

Cyp3a11     mouse
            PIR A60564 (24 amino acids)
            Bornheim, L.M. and Correia, M.A.
            Purification and characterization of a mouse liver cytochrome
            P-450 induced by cannabidiol.
            Mol. Pharmacol. 36, 377-383 (1989)

CYP3A12     dog
            PIR S04341 (28 amino acids)
            Ciaccio, P.J. and Halpert, J.R.
            Characterization of a phenobarbital-inducible dog liver
            cytochrome P450 structurally related to rat and human
            enzymes of the P450IIIA (steroid-inducible) gene subfamily.
            Arch. Biochem. Biophys. 271, 284-299 (1989)

Cyp3a13     mouse
            GenEMBL X63023(1881bp)
            Yanagimoto,T., Itoh,S., Sawada,M., Hashimoto,H. and Kamataki,T.
            Molecular cloning and functional expression of a mouse cytochrome
            P-450 (Cyp3a-13): examination of Cyp3a-13 enzyme to activate
            aflatoxin B1 (AFB1).
            Biochim. Biophys. Acta 1201 (3), 405-410 (1994)

CYP3A14     Cavia cobaya (guinea pig)
            GenEMBL D16363 (1827bp)
            Mori,T., Itoh,S. and Kamataki,T.
            cDNA and deduced amino acid sequence of a novel cytochrome
            P450 from male guinea pig liver mRNA with high homology to 
            CYP3a family
            unpublished (1993)

CYP3A14     Cavia porcellus (guinea pig)
            GenEMBL D49731 (2321bp)
            Mori,T., Itoh,S. and Kamataki,T.
            Differential effects of ascorbic acid deficiency on the expression
            of the forms of cytochrome P450.
            Unpublished (1995)

CYP3A15     Cavia cobaya (guinea pig)
            GenEMBL D26487 (2163bp)
            Mori,T., Itoh,S. and Kamataki,T.
            Differential effects of ascorbic acid deficiency on the expression
            of the forms of cytochrome P450.
            Unpublished (1995)

Cyp3a16     mouse
            GenEMBL D26137 (1701bp)
            Itoh,K., Satoh,M., Abe,Y., Hashimoto,H., Ynagimoto,T. and Kamataki T.
            A novel form of mouse cytochrome P450 3A (Cyp3a16). Its cDNA cloning 
            and expression in fetal liver.
            Eur. J. Biochem. 226, 877-882 (1994)

CYP3A17     guinea pig 
            GenEMBL D28515 
            Mori,T.
            submitted to Nomenclature Committee 
            Not in Genbank as of 6/26/95

CYP3A18     rat
            no accession number
            Kiyoshi Nagata
            6-beta-C
            submitted to Nomenclature Committee 

CYP3A18     rat
            GenEMBL X79991 (2005bp)
            Strotkamp,D., Roos,P.H. and Hanstein,W.G.
            A novel CYP3 from female rats.
            Biochim. Biophys. Acta 1260, 341-344 (1994)

CYP3A18     rat
            GenEMBL D38381 (?bp) (not in GenBank yet,1/30/97)
            Yamazoe,Y. et al. 
            Isolation and characterization of of a new rat P450 (CYP3A18) cDNA 
            encoding P4506b-2 catalyzing testosterone 6b- and 16a-hydroxylations.
            Pharmacogenetics in press (1995)

CYP3A19     Capra hircus (goat)
            GenEMBL X76503 (1084bp)
            Zeilmaker,W.M., Van 't Klooster,G.A.E., Gremmels-Gehrmann,F.J.,
            Van Miert,A.S.J.P.A. and Horbach,G.J.M.J.
            cDNA and deduced amino acid sequence of a dwarf goat liver
            cytochrome P450-fragment belonging to the CYP2C gene subfamily
            Biochem. Biophys. Res. Commun. 200, 120-125 (1994)
            C-terminal fragment from I-helix to end

CYP3A20     Guinea pig 
            GenEMBL 
            T. Mori
            unpublished

CYP3A21     Marmoset
            GenEMBL 
            Igarishi, T. 
            unpublished

CYP3A22     Miniature pig
            Shimojima, T.
            unpublished

CYP3A23     rat 
            GenEMBL D13912
            Kirita,S. and Matsubara,T.
            Cloning and characterization of a novel member of steroid induced 
            cytochrome P450 3A in rats.
            Arch. Biochem. Biophys. 307, 253-258 (1993)

CYP3A23     rat 
            GenEMBL D29967
            Komori,M. and Oda,Y.
            A major glucocorticoid inducible P450 in rat liver is not P4503A1
            J. Biochem. 116, 114-120 (1994)

CYP3A24     sheep
            GenEMBL U59378(1653bp)
            Ching,M.S., Chun-Jing,J., Ghabrial,H., Wookey,P.J., Smallwood,R.A.
            and Morgan,D.J.
            Ovine foetal liver CYP3A24
            unpublished (1996)

Cyp3a25     mouse
            GenEMBL AF204959
            Dai,D., Bai,R., Hodgson,E. and Rose,R.L.
            submitted to Nomenclature Committee 10/18/96
            Cloning, sequencing, heterologous expression and characterization
            of murine Cytochrome P450 3A25, a 6B-Testosterone hydroxylase
            Unpublished

Cyp3a25     mouse
            GenEMBL Y11995
            Mahnke,A., Roos,P. and Hanstein,W.G.
            one amino acid difference with Roses sequence at amino acid 215
            unpublished

CYP3A26     dog
            no accession number
            David Fraser
            submitted to Nomenclature Committee 
            95.6% identical to 3A12 from dog

CYP3A27     Oncorhynchus mykiss (rainbow trout)
            GenEMBL U96077
            Don R. Buhler
            Arch. Biochem. Biophys. 360, 000-000 (1998) in press
            56% identical to 3A12

CYP3A28     bovine
            GenEMBL Y10214(1870bp)
            Natsuhori,M., van Raak,M., Ligtenberg,M., Kleij,L., ten Berge,D.,
            Zweers-Zeilmaker,W.M., de Groene,E.M., Van Miert,A.S.J.P.A.,
            Witkamp,R.F. and Horbach,G.J.
            Isolation of a full length cytochrome P450 (CYP3A) cDNA sequence
            and its functional expression in V79 cells.
            unpublished (1997)

CYP3A29v1   pig
            GenEMBL Z93099 (2015bp)
            Nissen,P.H., Winteroe,A.K. and Fredholm,M.
            Characterization and mapping of three porcine genes belonging to
            the cytochrome P450 superfamily
            Unpublished
MDLIPGFSTETWVLLATSLVLLYLYGTYSHGLFKKLGIPGPRPL
PYFGNILGYRKGVDHFDKKCFQQYGKMWGVYDGRQPLLAVTDPNMIKSVLVKECYSVF
TNRRSFGPLGAMRNALSLAEDEEWKRIRTLLSPTFTSGKLKEMFPIISHYGDLLVSNL
RKEAEKGKPVTMKDIFGAYSMDVITSTAFGVNIDSLNNPQDPFVENSKKLLKFSFFDP
FLLSLIFFPFLTPIFEVLNITLFPKSSVNFFTKSVKRMKESRLTDQQKRRVDLLQLMI
NSQNSKEMDPHKSLSNEELVAQGIIFIFAGYETTSSALSLLAYELATHPDVQQKLQEE
IEATFPNKAPPTYDALAQMEYLDMVVNETLRLYPIAARLERACKKDVEIHGVFVPKGT
VVVVPVFVLHRDPDLWPEPEEFRPERFSKKHKDTINPYTYLPFGTGPRNCIGMRFALM
NMKLALVRVLQNFSFKPCKETQIPLKLTTQGLTQPEKPVVLKILPRDGTVSGA

CYP3A29v2   Sus scrofa (pig)
            No accession number 
            Misaki Kojima
            Submitted to nomenclature committee Oct. 27, 2000
            2 amino acid diffs with 3A29v1
              clone name 3Ac9

CYP3A29v3   Sus scrofa (pig)
            No accession number 
            Misaki Kojima
            Submitted to nomenclature committee Oct. 27, 2000
            1 amino acid diff with 3A29v1
              clone name 3Ac16

CYP3A29v4   Sus scrofa (pig)
            No accession number 
            Misaki Kojima
            Submitted to nomenclature committee Oct. 27, 2000
            1 amino acid diff with 3A29v1, 2 aa diffs with 3A29v3
              clone name 3Ac25

CYP3A29v5  Sus scrofa (mini pig)
     GenEMBL AF424780
     Soucek,P., Zuber,R., Anzenbacherova,E., Linka,M. and Anzenbacher,P.
     cDNA of minipig cytochrome P450 3A
     Unpublished
     75% to 3A4 human 98% to 3A29v1 8 diffs.  
     3 diffs with 3A29v3 and v4 (Partial seqs from 99-328)
     4 diffs with 329v2 (Partial seq from 99-328)
     
CYP3A30     Fundulus heteroclitus
            Genbank AF105068
            Malin Celander and John J. Stegeman
            Isolation of a cytochrome P450 3A cDNA sequence (CYP3A30) from the 
            marine teleost Fundulus heteroclitus and phylogenetic analyses of CYP3A 
            genes. 
            Biochem. Biophys. Res. Commun. 236, 306-312 (1997)

CYP3A31     Syrian hamster
            GenEMBL D86951
            Alabouch,S., Kurose,K., Tohkin,M., Bani,M.H., Fukuhara,M.,
            Nagata,K. and Yamazoe,Y.
            cDNA cloning and expression of a novel CYP3A from the Syrian
            hamster, CYP3A31
            Biochim. Biophys. Acta 1397, 9-13 (1998)
            clone name P450SH3A-1

CYP3A32     Balaenoptera acutorostrata  (Minke whale)
            no accession number
            Ikuko Teramitsu, Yukio Yamamoto, and Shoichi Fujita
            submitted to nomenclature committee

CYP3A33     Phocoenoides dalli (Dall's porpoise)
            no accession number
            Ikuko Teramitsu, Yukio Yamamoto, and Shoichi Fujita
            submitted to nomenclature committee

CYP3A34     Eumetopias jubatus (Steller sea lion)
            no accession number
            Ikuko Teramitsu, Yukio Yamamoto, and Shoichi Fujita
            submitted to nomenclature committee

CYP3A35     Phoca largha (Spotted seal)
            no accession number
            Ikuko Teramitsu, Yukio Yamamoto, and Shoichi Fujita
            submitted to nomenclature committee

CYP3A36     Phoca fasciata (Ribbon seal)
            no accession number
            Ikuko Teramitsu, Yukio Yamamoto, and Shoichi Fujita
            submitted to nomenclature committee

CYP3A37     Gallus gallus (chicken)
            no accession number
            Manuel Baader and Urs Meyer
            submitted to nomencalture committee
            60% identical to mammalian CYP3A sequences

CYP3A38     Medaka (fish)
            no accession number
            Seth Kullman
            submitted to nomenclature committee 11/6/98
            72% identical to CYP3A27 trout

CYP3A39v1   Sus scrofa (pig)
            GenEMBL AF109068
            Schuetz,E., Andag,R., Wieland,E. and Oellerich,M.
            Porcine Cytochrome-P450 with high protein homology to human CYP3A4
            (Nifedipine oxidase J04449)
            Unpublished
            88% identical to 3A29
MDLIPGFSTETWVLLATSLVLLYLYGTYSHGLFKKLGIPGPRPL
PYFGNILGYRKGVDHFDKKCFQQYGKMWGFFDGRQPVLAITDPDMIKTVLVKECYSVF
TNRRSFGPRGAMRTALSLAEDEEWKRIRTLLSPTFTSGKLKEMFPIISHYGDLLVSNL
RKEAEKGKPVTMKDIFGAYSMDVITSTAFGVNTDFLNNPQDPFVENSKKLLKFSFFSP
LFLSIIFFPFLTPILEVLNVTLFPKSVVNFFMRSIKRMKESRLKDKQTHRVDFLQLMI
NSQNSKETDTHKGLSDEELVAQGVFFIFAGYETTSSSLSLLVYELATHPDVQQKLQEE
IDATFPSKALPSYDALAQMEYLDMVVNEILRLYPIAARLERVCKKDVEIHGVSVPKGT
VMMVPVFSIHRDPELWPEPEEFRPERFSKKNKDSINPYTYLPFGTGPRNCIGMRFALM
NMKLALVRVLQNFSFKPCKETQTPLKLSSQGLIQPEKPILLKVVPRDGTVGGA

CYP3A39v2   Sus scrofa (pig)
            No accession number 
            Misaki Kojima
            Submitted to nomenclature committee Oct. 27, 2000
            3 amino acid diffs with 3A39v1, 1 aa diff with 3A39v3
              clone name 3Ac13

CYP3A39v3   Sus scrofa (pig)
            No accession number 
            Misaki Kojima
            Submitted to nomenclature committee Oct. 27, 2000
            1 amino acid diff with 3A39v2, 2 aa diffs with 3A39v1
              clone name 3Ac19

CYP3A39v4   Sus scrofa (pig)
            No accession number 
            Misaki Kojima
            Submitted to nomenclature committee Oct. 27, 2000
            4 aa diffs with 3A39v3, 6 aa diffs with 3A39v1
              clone name 3Ac32

CYP3A40     medaka
            no accession number
            Seth Kullman
            submitted to nomenclature committee 1/12/99
            90% identical to CYP3A38 trout

Cyp3a41     mouse 
            GenEMBL NM_017396 AB033414
            Sakuma,T., Takai,M., Endo,Y., Kuroiwa,M., Ohara,A., Jarukamjorn,K.,
            Honma,R. and Nemoto,N.A novel female-specific member of the CYP3A gene 
            subfamily in the mouse liver
            Arch. Biochem. Biophys. 377 (1), 153-162 (2000)            
            91% identical to 3a11 87% identical to 3a16 69% to 3a25 and 
            68% to 3a13 90% to 3a44
            submitted to nomenclature committee 6/1/99

CYP3A42     Python regius (ball python or royal python, African snake) 
            No accession number
            Mette Liljenberg, Tove Hegelund-Myrback and Malin Celander
            Submitted to nomenclature committee Oct 22, 1999
            59% to mouse 3a

CYP3A43     human
            GenEMBL AC011904 8902-46787 13 exons
            Gene assembled from genomic sequence by Henry Strobel 
            and David Nelson on Dec 11, 1999
            intron exon boundaries defined by comparison to rat 3A9
            and human 3A sequences.  GT AG pairs found for all introns
            ESTs AA417369 zu08d03.s1 AA416822 zu08d03.r1 Soares testis
            Opposite ends of same clone
            67% identical to rat 3A9

Assembled gene * = intron exon boundary ** = EST support for this boundary

MDLIPNFAMETWVLVATSLVLLYI*
YGTHSHKLFKKLGIPGPTPLPFLGTILFYLR*
GLWNFDRECNEKYGEMWG*
LYEGQQPMLVIMDPDMIKTVLVKECYSVFTNQM*
PLGPMGFLKSALSFAEDEEWKRIRTLLSPAFTSVKFKE*
MVPIISQCGDMLVRSLRQEAENSKSINLKE*
DFFGAYTMDVITGTLFGVNLDSLNNPQDPFLKNMKKLLKLDFLDPFLLLI* 
SLFPFLTPVFEALNIGLFPKDVTHFLKNSIERMKESRLKDKQK*
HRVDFFQQMIDSQNSKETKSHK*
ALSDLELVAQSIIIIFAAYDTTSTTLPFIMYELATHPDVQQKLQEEIDAVLPNK**
APVTYDALVQMEYLDMVVNETLRLFPVVSRVTRVCKKDIEINGVFIPKGLAVMVPIYALHHDPKYWTEPEKFCPE**
RFSKKNKDSIDLYRYIPFGAGPRNCIGMRFALTNIKLAVIRALQNFSFKPCKETQ**
IPLKLDNLPILQPEKPIVLKVHLRDGITSGP*

coding region 504 amino acids

exon 1 8902-8972
exon 2 17239-17332
exon 3 19906-19958
exon 4 24929-25028
exon 5 28274-28387
exon 6 28952-29040
exon 7 30332-30480
exon 8 36377-36504
exon 9 37619-37685
exon 10 40616-40776
exon 11 42399-42625
exon 12 44323-44485
exon 13 46692-46787

AC011904 alt exon 1 upstream of 3A43 83% to 3A43 exon 1
MDLIPNFAMEMCVLLATSLVLLYL

Cyp3a44     mouse
            GenEMBL AB039380
            Tsutomu Sakuma
            Submitted to nomenclature committee 2/29/2000
            This sequence differs from 3a11, 3a13, 3a16, 3a25 and 3a41 (90%)

CYP3A45     Oncorhynchus mykiss (rainbow trout)
            No accession number
            Su-Jun Lee, Don R. Buhler 
            96% to 3A27 but different 3 prime and 5 prime ends

CYP3A46v1   Sus scrofa (pig)
            No accession number 
            Misaki Kojima
            Submitted to nomenclature committee Oct. 27, 2000
            13 diffs with 3A39v1, 9 diffs with 3A46v2 all in the last 43 aa
            clone name 3Ac4

CYP3A46v2   Sus scrofa (pig)
            No accession number 
            Misaki Kojima
            Submitted to nomenclature committee Oct. 27, 2000
            9 diffs with 3A46v1 all in the last 43 aa, 20 diffs with 3A39v1
            clone name 3Ac28

CYP3A47X    Fugu rubripes (pufferfish)
            No accession number
            Scaffold 600
            Renamed CYP3D1
      MNYLPLFALETWILLITFTCLFVM (2)
      YGKRTFGIFEKLGISGPKPTMYFGSICKYNN (0)
14338 VYYLDQECAQKYGKIWG (2)
14472 TYELRKPMLVVMDPDLLKTILVKECFTHFTNRR (0)
      NFCLNGDLYDAVNIAEDDDWRRIRNVLSPLFTTGRIKQ 14879 (0)
      IFSLMKRQSSKLTSSLEPKAENEEIISIKD (2)
      FFGAYNMNVATGVLFGMEMEPSLPIKHASKLFKFPIPPLHDPSTSP (1)
      Cannot identify exon 8 in this region
  (0) EFDFLQHMISTQKNDGIYAVTS
  (1) GLTDHEIVSQLTVLLTGGYETSTLALTLSIYSLATNPGSMNRLQEEIDATFPDA (0)
16040 APVTYEALMQMEYVDCVINECLR (2)
      LYPPAARLERTAKETVEISGITIPKNMTVTVPIFALHRDPEHWPEPEEFKPDR 16334 (2)
      FSKQNKGRINPYTYLPFGIGPRKCLGMRLALVIVKLALVETLQKYSFSVCKETE (0)
      IPFKMDPNTFVGPINPIKLRVVRR*

CYP3A48     Fugu rubripes (pufferfish)
            No accession number
            Scaffold 1124a
44660 MDLLLNFSIETWTLLTLVFTLIIV 44589 (2)
44089 YGYAPYGFFKKVGIPGPKPWPFIGTFLNYRR 43997 (0)
43888 GVHHFDEECYKKYGKVWG 43835 (2)
43728 LYDGRQPLMCIMDTGIIKTILVKECYSNFTNRR 43630 (0)
43549 DLGLNGPLRDAVSVAEDEQWKRIRGILSPSFTSGRLKE 43436 (0)
43350 MYTIMLQHSKNLLNFLHKKVEADEVIDVKD 43261 (2)
42808 VFGPYSMDVVTSTAFSADIDSINHPSDPFVANIKKMVKFNFLNPLLIFV 42662 (1)
42612 VLFPFTQPIFDKVDFSFFPAHVIDFFYNFLRQIKSDRNKDKKK 42452 (0)
42378 SRVDFMQLMVNAQMQEGNEEGSSQK 42310 (1)
42109 GLTDNEILAQAMIFIFAGYETTSSSLGFLAYNLATNPKIQKKLQEEIDKTFPGK 41948 (0)
41869 VRPNYDDLMQLEYLDMVVNESMRVFPILSRLERMTKTSVEI 41747
41746 NGFTIPKGTVVAIPVYVLQHDKAYWPEPEAFKPER 41648 (2)
41162 FSKENKDNVDPYAYLPFGAGPRNCIGNRFALVLMKLAIAEILQHYSFVPCKETD 41001 (0)
40889 IPMVLNTEGLVAPKNPIKLKLKPRAVSS* 40803

CYP3A49     Fugu rubripes (pufferfish)
            No accession number
            Scaffold 1124b length 48103 gene runs off end of scaffold
48101 XXXXYEILAQ 48804 frameshift
48084 AIFFIFAGYELPAALLDFANNLATNPKIQKKLQEEIDKTFPGK 47956 (0)
47876 VRPNYDDLMQLEYLDMVVNESMRLYPIANRLERMTKTSVEINGLTIPKGTVVAIPVYALQ 47697
47696 RDPVLWPEPEAFKPER 47648 (2)
47492 FSKENKDNVDPYAYLPFGAGPRNCIGNRFALVLMKLAIAEILQHYSFVTCQETD (0) 47331
47246 IPMVLGNEGFVTPKNPIKLKLKLRDVFN* 47160

CYP3A50P    Fugu rubripes (pufferfish)
            No accession number
            Scaffold 10760 probable pseudogene
1847 MNFLPDFFLNTWTFLILVIVLSTT 1918 (2) exon 1
2258 bad boundary MDVLHMSSIKRVGIRRPKTWPIIGTYNRTDG 2350 bad boundary exon 2
     cannot identify exons 3-6
3091 XXXXXXXXXXXXXXFSIDIESLNNPSSPFLHYLQEVSKYNYMNLWRLLG 3195 (1) part of exon 7
3268 AIFPFLTPLMD 3300 start of exon 8 
     Seq gap in scaffold
3919 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYNLAVHPQTQTRLQAEIDRTFPGK 3990 exon 10
4078 CLITYEELLQLKYLDMVVTETSRLYPLGNRIERVAKSTVEVSGVIIPEGVAVAVPIYTLH 4257
4258 RDPTVWPDPDSFKPERFSKDNRDNIDPYGLLTR 4424 frameshift and 7 nuc. deletion 
4423 GPRSCTGTRMSMLVVKLTLVEILQHFSFVACKETM 4527
     IPMVLDDNGFVHPKTPIMLIPARTPGSCFSACTYVINVLLTVRRLICSLIN* 4787

CYP3A51P   human
           NT_033964.1|Hs7_34119 chromosome 7 
           lone exon 13 upstream of 3A5
254205 QIPLKLRLGGLLQPEKPIVLKVESRDGTVSG 254297 ex13 

CYP3A52P   human
           AC011904 part of exon 4 downstream of CYP3A43, 88% to 3A4 
MIKTVLVKECYSVFPNWR

CYP3A53P   human
           NT_033964.1|Hs7_34119 Homo sapiens chromosome 7 
           lone exon 6 downstream of 3A43
67275 EMLLIIL*YGDVFLRNLRQKVEKGKPLTLR 67186 ex 6

CYP3A54P   human
           NT_033967.1|Hs7_34122 chromosome 7 
           3A psuedogene Exon 2
2460622 LYRYGTCSHGLFKKMAIPGLTLLPF 2460548

CYP3A55P   human
           NT_033967.1|Hs7_34122 chromosome 7 
           3A psuedogene Exon 5
2246484 LESGLIITKHEKWKRLRSVISPAFPSGELQEV 2246579

CYP3A56    Fundulus heteroclitus (killifish)
           No accession number
           Tove Hegelund and Malin Celander
           64% to 3A48 Fugu 99% to 3A30
           submitted to nomenclature committee 6/20/02

Cyp3a57    mouse 
           GenEMBl AC115895.3, XM_144630 XP_144630 Genbank assembly incorrect
MELIPNLSIQTWVLLVTSLVFFYI
YGTYSHGLFKKLGIPGPKPLPLFGNIINYLD
GMWKFDDDCYKKYGKIWG
FYEGPQPILAIMDPEIIKIVLVKECYSVFTNRR
PFGPVGFLKKSITISEDEEWKRLRTLLSPTFTSSKLKE
MFPIMRQYGDILVRNLRREEEKEEPINMK
DIFGAYSMDVITGTSCGVNIDSLNNPQDPFVQKIKKILKFKFFDPFLLS 
VVLFPFLTPIYEMLNLSIFPRQSMNFFKKFVKKMKKDRLDYNQK
NRVDFLQLMMNTQNSKGQESLK
ALSDLEMAAQTLIFIFGGYDGTSTSISFIMYELATHPDVQKKLQDEIDRALPNK
APVTYDALMDMEYLDMVVNESLRLYPVVSRIDRFSKKDVEIHGVFIPKGTIVMIPIYPLHRDPEYWPEPEDFCPER
FSKENKGNIDPYIYMPFGNGPRNCIGMRFALISVKLAVTGVLQNFTVQPCEETQ
IPVKISRQPIFRPEKPIILKFVSRDKPRTGP

Cyp3a58-ps  mouse
            See mouse page

Cyp3a59     mouse
            See mouse page

Cyp3a60-ps  mouse
            See mouse page

Cyp3a61-ps  mouse
            See mouse page

CYP3A62     rat
            GenEMBL AB084894 
            Matsubara T, Kim H. J, Miyata M, Shimada M, Nagata K and Yamazoe Y.
            Isolation and characterization of a novel rat CYP3A, CYP3AL1, 
            predominantly expressed in intestinal tract  Unpublished 2003
            80% to CYP3A9, 78% to Cyp3a13
MDLIPNISLETWMLLATILVLLYLYGTSTHGNFKKLGISGPKPL
PFVGNILAYRHGFWEFDRHCHKKYGDIWGFYEGRQPILAITDPDIIKTVLVKECYSTF
TNRRSFGPAGILKKAITLSEDEEWKRLRTLLSPTFTSGKLKEMFPIINQYADLLVKNV
KHEAEKGNPITMKDIFGAYSMDVITGTSFGVNVDSLNNPQNPFVQKVKKLLKFNFLDP
FFLSVILFPFLTPVFEAFDITVFPKDVMKFFRTSVERMKENRMQEKVKQRLDFLQLMI
NSQSSGDKESHQGLTDVEIVAQSIFFIFAGYETTSSALSFALYLLATHPDLQKKLQDE
IDAALPNKAPVTYDVLVEMEYLDMVLNETLRLFPVGGRLERVCKKDVEINGVFIPKGT
VVMVPTFALHKDPKCWPEPEEFCPERFRKKNQDSINPYIYLPFGNGPRNCIGMRFALM
NMKIALVRVLQNFSFGLCKETQIPLKLRKKGFFQPEKPIILRAVSRD

Cyp3a63-ps  mouse
            See mouse page

CYP3A64     Macaca mulatta (rhesus monkey)
            No accession number
            Tom Rushmore, Merck & Co. Inc.
            Submitted to nomenclature committee 6/30/2003
            92% to CYP3A4

CYP3A65     Danio rerio (zebrafish)
            No accession number
            Hua-Bin Tseng, Hou-Chu Yin, Chin-Hwa Hu, 
            Jun-Lan Wang-Buhler, and Donald R. Buhler
            Submitted to nomenclature committee 10/10/2003
            71% to 3A27 trout, 71% to 3A45 trout, 69% to 3A40 Oryzias, 
            69% to 3A30 Fundulus, 69% to 3A56 fundulus,
            56% to 3A37 chicken, 58% to 3A48 fugu  59% to 3A49 fugu, 45% to 3B2

CYP3A       sheep
            PIR A60728 (20 amino acids)
            Pineau, T., Galtier, P., Bonfils, C., Derancourt, J. and Maurel,P.
            Purification of a sheep liver cytochrome P-450 from the
            P450IIIA gene subfamily. Its contribution to the
            N-dealkylation of veterinary drugs.
            Biochem. Pharmacol. 39, 901-909 (1990)
            only one amino acid difference with CYP3A24   (L9K)

CYP3A       baboon (Papio sp.)
            Swiss P80056 (20 amino acids) PIR S21176 (20 amino acids)
            Purification of two cytochrome P450 isozymes related to CYP2A
            and CYP3A gene families from monkey (baboon, Papio papio)
            liver microsomes. Cross reactivity with human forms.
            Dalet-Beluche I., Boulenc X., Fabre G., Maurel P., Bonfils C.
            Eur. J. Biochem. 204, 641-648 (1992)

CYP3B1      Fugu rubripes (pufferfish)
            No accession number
            Scaffold_2213a
6382   MFEFVLFSGTTWALLALFFALLLL 6311 (2)
6232   YGVWPYHHFKKLGIRGPRPLPFMGSTFYYRK 6134 (0)
6052   GIIPFESWCQAEYGDVWG 5999 (2)
       Exon 3 not detected may be in small seq. gap
5469   XFAGPLEDSVSAVKDERWKRIRSTISPCFTSGRLKN 5368 (0)
5259   AFPIVARYADRITKKLEQSNLDEPINVKE 5173 (2)
5069  FLAPYSLDAVTSVSFSVEADSINNPNDPLIVNLKKVFKFNFVVFFLV 4929 (1)
4846   AFFPFCARLFQFLGIDPIPRSSVNYFYNVIKNFKDQHHAD 4727 (bad boundary, 0 expected)
4124 TRGDFLQVLIQSEIPQSEIKSEQDQPPK 4041 (1)
4011  GLTEHEILSQAFIFIFGGYETTTTTLTNVLYGLAINPDVLQVLHKEIDTNIPSD 3850 (0)
3780   APISYEDLMGLQYLDQVLNESQRLYPTAPRLERACKKTVQIHG 3652
3651  LTILEGTIVGIPVHLLHKDPRFWSSPEEFRPER 3553 (2)
3416  FSKDSTEEVNPYAFMPFGLGPRNCVGMRYAILVMKMLIVRLLQSYTVETCKDTM 3252 (0)
3171   IPLEFDWKSQPLKPIKLSFIPRQK* 3097

CYP3B2      Fugu rubripes (pufferfish)
            No accession number
            Scaffold_2213b
12175 MFVFMCFSATTWTILVLFSTLLLL (2)
11998 YGHWPYRLFRKNGIPGPMPLPFIGTMWNLLK 11906 (0)
11778 GNMVFDRECQSKYGDVWG 11725 (2)
11412 VFEGRTPVLMVSDPGMIKTILVKECYSVFTNHRVR (0)
11044 IFSGPLETSVFSAKDETWKRMRTSISPCFTSGRLRQ (0)
10847 AFPIIARYADRFIAKLEQTKLEDSTDIKK 10731 (2)
10640 LFGPYSLDVIASSSFSVDADSINNPDDPFIINIKKVLNLNFWLLLIK 10500 (1)
10422 NVLPFSSYLFEFLHIDIIPRSIVDYFFNLIKQLKAQHDQS 10309 (0)
 9911 IKGDFLHVMIQNEIPQSEIKSDQDQPPK (1)
 8638 GLTEQEILSQSVLFIFGGYDTTTITITYLLYNLAINPDVLQILHSEIDSNFPKD 8483 (0)
 8391 TPFSYEDLVGFQYLDQVLNESQRLIPTAPALERFCKKTVQIHGLTIPEGTVVAVPVHLL 8215
 8214 HKDPRFWSSPEEFRPER 8164 (2)
      XXXXXXXXX in sequence gap 
      IELGTFMX frameshift
 8001 FGLGPRNCIGMRYAILVMKMILVRVLQSYTVETCKDTM 7888 (0)
 7808 IPLEFDWKLQPTKQIKLRLVPRQK* 7734

CYP3C1      Danio rerio (zebrafish)
            GenEMBL BC052130.1 dated 10/7/2003
            Also submitted by Don Buhler 10/2/2003
            Jun-Lan W. Buhler, Thuy Hoang, Quan D. Nguyen, 
            Chin-Hwa Hu, and Donald R. Buhler
            52% to 3A48, 53% to 3B1 fugu, 51% to 3B2 fugu 52% to 3A49 
            53% to 3A27 trout, 54% to 3A40 Oryzias, 53% to 3A45 trout, 
            54% to 3A56 fundulus, 54% to 3A30 fundulus, 49% to 3A37 chicken, 
            48% to 3A29 pig (47% to 50% to many mammal 3As)

CYP3D1      Fugu rubripes (pufferfish)
            No accession number
            Scaffold 600
            Formerly CYP3A47
      MNYLPLFALETWILLITFTCLFVM (2)
      YGKRTFGIFEKLGISGPKPTMYFGSICKYNN (0)
14338 VYYLDQECAQKYGKIWG (2)
14472 TYELRKPMLVVMDPDLLKTILVKECFTHFTNRR (0)
      NFCLNGDLYDAVNIAEDDDWRRIRNVLSPLFTTGRIKQ 14879 (0)
      IFSLMKRQSSKLTSSLEPKAENEEIISIKD (2)
      FFGAYNMNVATGVLFGMEMEPSLPIKHASKLFKFPIPLFMIQGASP (1)
      RCFPILLPLLQLMGFSLFPRDSFAFVKKIVEKIRAERDGGSHQ (0)
      EFDFLQHMISTQKNDGIYAVTS
  (1) GLTDHEIVSQLTVLLTGGYETSTLALTLSIYSLATNPGSMNRLQEEIDATFPDA (0)
16040 APVTYEALMQMEYVDCVINECLR (2)
      LYPPAARLERTAKETVEISGITIPKNMTVTVPIFALHRDPEHWPEPEEFKPDR 16334 (2)
      FSKQNKGRINPYTYLPFGIGPRKCLGMRLALVIVKLALVETLQKYSFSVCKETE (0)
      IPFKMDPNTFVGPINPIKLRVVRR*

4A Subfamily

CYP4A1      rat
            PIR S21711 (22 amino acids)
            Okita, R.T. and Okita, J.R.
            Characterization of a cytochrome P450 from Di(2-ethylhexyl)
            phthalate-treated rats which hydroxylates fatty acids.
            Arch. Biochem. Biophys. 294, 475-481 (1992)

CYP4A1      rat 
            Aldridge et al. 
            Biochem. J. 306, 473-479 (1995)
            note: identification of far upstream PPAR response element.

CYP4A1      rat
            GenEMBL M14972
            Hardwick,J.P., Song,B.-J., Huberman,E. and Gonzalez,F.J.
            Isolation, complementary DNA sequence, and regulation of rat
            hepatic lauric acid omega-hydroxylase (cytochrome P-450-LA-omega):
            Identification of a new cytochrome P-450 gene family
            J. Biol. Chem. 262, 801-810 (1987)

CYP4A1      rat
            GenEMBL X07259
            Earnshaw,D., Dale,J.W., Goldfarb,P.S. and Gibson,G.G.
            Differential splicing in the 3' non-coding region of rat cytochrome
            P-452 (P450 IVA1) mRNA
            FEBS Lett. 236 (2), 357-361 (1988)

CYP4A1      rat
            GenEMBL M33937 M57718
            Kimura,S., Hanioka,N., Matsunaga,E. and Gonzalez,F.J.
            The rat clofibrate-inducible CYP4A gene subfamily I. Complete
            intron and exon sequence of the CYP4A1 and CYP4A2 genes, unique
            exon organization, and identification of a conserved 19-bp upstream
            element
            DNA 8, 503-516 (1989)

CYP4A2      rat
            GenEMBL M33938 M57719
            Kimura,S., Hanioka,N., Matsunaga,E. and Gonzalez,F.J.
            The rat clofibrate-inducible CYP4A gene subfamily I. Complete
            intron and exon sequence of the CYP4A1 and CYP4A2 genes, unique
            exon organization, and identification of a conserved 19-bp upstream
            element
            DNA 8, 503-516 (1989)

CYP4A3      rat
            GenEMBL M33936
            Kimura,S., Hardwick,J.P., Kozak,C.A. and Gonzalez,F.J.
            The rat clofibrate-inducible CYP4A subfamily II. cDNA sequence of
            IVA3, mapping of the Cyp4a locus to mouse chromosome 4, and
            coordinate and tissue-specific regulation of the CYP4A genes
            DNA 8, 517-525 (1989)

CYP4A4      rabbit
            GenEMBL L04758 (1378bp) PIR S32315 (510 amino acids)
            PIR S32423 (64 amino acids)
            Palmer,C.N., Griffin,K.J. and Johnson,E.F.
            Rabbit prostaglandin omega-hydroxylase (CYP4A4):
            Gene structure and expression.
            Arch. Biochem. Biophys. 300, 670-676 (1993)
            Note: The translation is different from the 4A4 sequence
            in the alignment after AALLG. A frameshift occurs between
            nucleotides 1201 and 1203, with an extra "G" added.  After 1320
            P450 similarity stops.  1320-1378 is an intron sequence.

CYP4A4      rabbit
            PIR JN0089 (20 amino acids)
            Yoshimura, R., Kusunose, E., Yokotani, N., Yamamoto, S.,
            Kubota, I. and Kusunose, M.
            Purification and characterization of two forms of fatty acid
            omega-hydroxylase cytochrome P-450 from rabbit kidney
            cortex microsomes.
            J. Biochem. 108, 544-548 (1990)

CYP4A4      rabbit 
            GenEMBL J02818
            Matsubara,S., Yamamoto,S., Sogawa,K., Yokotani,N.,
            Fujii-Kuriyama,Y., Haniu,M., Shively,J.E., Gotoh,O., Kusunose,E.
            and Kusunose,M.
            cDNA cloning and inducible expression during pregnancy of the mRNA
            for rabbit pulmonary prostaglandin omega-hydroxylase (cytochrome
            P-450p-2)
            J. Biol. Chem. 262, 13366-13371 (1987)

CYP4A5      rabbit
            GenEMBL M28655
            Johnson,E.F., Walker,D.L., Griffin,K.J., Clark,J.E., Okita,R.T.,
            Muerhoff,A.S. and Masters,B.S.
            Cloning and expression of three rabbit kidney cDNAs encoding lauric
            acid omega-hydroxylases
            Biochemistry 29, 873-879 (1990)

CYP4A5      rabbit
            GenEMBL X57209
            Yokotani,N., Kusunose,E., Sogawa,K., Kawashima,H., Kinosaki,M.,
            Kusunose,M. and Fujii-Kuriyama,Y.
            cDNA cloning and expression of the mRNA for cytochrome P-450kd
            which shows a fatty acid omega-hydroxylating activity
            Eur. J. Biochem. 196 (3), 531-536 (1991)

CYP4A6      rabbit
            PIR S23949 (510 amino acids)
            Muerhoff, A.S., Griffin, K.J. and Johnson, E.F.
            Characterization of a rabbit gene encoding a
            clofibrate-inducible fatty acid omega-hydroxylase: CYP4A6.
            Arch. Biochem. Biophys. 296, 66-72 (1992)

CYP4A6      rabbit
            PIR PQ0047 (20 amino acids)
            Kikuta, Y., Kusunose, E., Okumoto, T., Kubota, I. and Kusunose,M.
            Purification and characterization of two forms of cytochrome
            P-450 with omega-hydroxylase activities toward
            prostaglandin A and fatty acids from rabbit liver
            microsomes.
            J. Biochem. 107, 280-286 (1990)

CYP4A6      rabbit 
            GenEMBL M29531 J05150
            Yokotani,N., Bernhardt,R., Sogawa,K., Kusunose,E., Gotoh,O.,
            Kusunose,M. and Fujii-Kuriyama,Y.
            Two forms of omega-hydroxylase toward prostaglandin A and laurate:
            cDNA cloning and their expression
            J. Biol. Chem. 264, 21665-21669 (1989)

CYP4A6      rabbit
            GenEMBL M28656
            Johnson,E.F., Walker,D.L., Griffin,K.J., Clark,J.E., Okita,R.T.,
            Muerhoff,A.S. and Masters,B.S.
            Cloning and expression of three rabbit kidney cDNAs encoding lauric
            acid omega-hydroxylases
            Biochemistry 29, 873-879 (1990)

CYP4A6      rabbit
            GenEMBL L04755
            Muerhoff,A.S., Griffin,K.J. and Johnson,E.F.
            Characterization of a rabbit gene encoding a clofibrate-inducible
            fatty acid omega-hydroxylase: CYP4A6
            Arch. Biochem. Biophys. 296, 66-72 (1992)

CYP4A7      rabbit
            PIR JN0090 (20 amino acids)
            Yoshimura, R., Kusunose, E., Yokotani, N., Yamamoto, S.,
            Kubota, I. and Kusunose, M.
            Purification and characterization of two forms of fatty acid
            omega-hydroxylase cytochrome P-450 from rabbit kidney
            cortex microsomes.
            J. Biochem. 108, 544-548 (1990)

CYP4A7      rabbit
            GenEMBL M29530 J05150
            Yokotani,N., Bernhardt,R., Sogawa,K., Kusunose,E., Gotoh,O.,
            Kusunose,M. and Fujii-Kuriyama,Y.
            Two forms of omega-hydroxylase toward prostaglandin A and laurate:
            cDNA cloning and their expression
            J. Biol. Chem. 264, 21665-21669 (1989)

CYP4A7      rabbit
            GenEMBL M28657
            Johnson,E.F., Walker,D.L., Griffin,K.J., Clark,J.E., Okita,R.T.,
            Muerhoff,A.S. and Masters,B.S.
            Cloning and expression of three rabbit kidney cDNAs encoding lauric
            acid omega-hydroxylases
            Biochemistry 29, 873-879 (1990)

CYP4A8      rat
            PIR S08300 (15 amino acids)
            Imaoka, S., Nagashima, K. and Funae, Y.
            Characterization of three cytochrome P450s purified from
            renal microsomes of untreated male rats and comparison with
            human renal cytochrome P450.
            Arch. Biochem. Biophys. 276, 473-480 (1990)

CYP4A8      rat
            GenEMBL M37828
            Stromstedt,M., Hayashi,S., Zaphiropoulos,P.G. and Gustafsson,J.A.
            Cloning and characterization of a novel member of the cytochrome
            P450 subfamily IVA in rat prostate
            DNA Cell Biol. 9 (8), 569-577 (1990)

Cyp4a10     mouse
            GenEMBL X69296 (1887bp)
            Colin J. Henderson, Theo Bammler & C. Roland Wolf
            Deduced amino acid sequence of a murine cytochrome P-450 Cyp4a 
            protein: developmental and hormonal regulation in liver and kidney
            Biochim. Biophys. Acta (1994) 1200, 182-190

Cyp4a10     mouse
            GenEMBL X71478 (129bp) PIR S35608 (43 amino acids)
            Bell,D.R., Plant,N.J., Rider,C.G., Na,L., Brown,S., Ateitalla,I.,
            Acharya,S.K., Davies,M.H., Elias,E.E., Jenkins,N.A.,
            Gilbert,D.J., Copeland,N.G. and Elcombe,C.R.
            Species-specific induction of cytochrome P-450 4A RNAs: PCR 
            cloning of partial guinea-pig, human and mouse cDNAs.
            Biochem. J. 294, 173-180 (1993)

            GenEMBL Y10221 (exons 10-12)
            Heng,Y.M., Kuo,C.W.S., Jones,P.S., Savory,R., Shultz,R.M.,
            Tomlinson,S.R., Gray,T.J.B. and Bell,D.R.
            A novel murine P-450 gene, Cyp4a14, is part of a cluster of Cyp4a
            and Cyp4b, but not of CYP4F, genes in mouse and humans.
            Biochem. J. 325, 741-749 (1997)
            Chromosomal assignment of this gene is given.

Cyp4a10     mouse
            GenEMBL AB018421
            Yasumura,N. and Ikeda,T.
            Polymorphism of cyp 4A10 sequence between C57BL/6 and ddY mouse
            Unpublished (1998)

CYP4A11     human
            GenEMBL L04751 (2576bp)
            PIR S29845 (519 amino acids)
            Palmer,C.N., Richardson,T.H., Griffin,K.J., Hsu,M.-H.,
            Muerhoff,A.S., Clark,J.E. and Johnson,E.F.
            Characterization of a cDNA encoding a human kidney,
            cytochome P450 4A fatty acid omega-hydroxylase and the
            cognate enzyme expressed in Escherichia coli.
            Biochim. Biophys. Acta 1172, 161-166 (1993)

CYP4A11     human
            GenEMBL X71480 (423bp) PIR S35609 (141 amino acids)
            Bell,D.R., Plant,N.J., Rider,C.G., Na,L., Brown,S., Ateitalla,I.,
            Acharya,S.K., Davies,M.H., Elias,E.E., Jenkins,N.A., 
            Gilbert,D.J., Copeland,N.G. and Elcombe,C.R.
            Species-specific induction of cytochrome P-450 4A RNAs: PCR 
            cloning of partial guinea-pig, human and mouse cDNAs.
            Biochem. J. 294, 173-180 (1993)

CYP4A11     human
            GenEMBL S67580 (2470bp) and S67581 (2469bp)
            Imaoka,S., Ogawa,H., Kimura,S. and Gonzalez,F.J.
            Complete cDNA sequence and cDNA-directed expression of
            CYP4A11, a fatty acid omega-hydroxylase expressed in human
            kidney.
            DNA Cell Biol. 12, 893-899 (1993)

            GenEMBL NM_000778
            Imaoka,S., Ogawa,H., Kimura,S. and Gonzalez,F.J.
            Complete cDNA sequence and cDNA-directed expression of CYP4A11, a
            fatty acid omega-hydroxylase expressed in human kidney
            DNA Cell Biol. 12, 893-899 (1993)

CYP4A11     human
            GenEMBL D26481 (2382bp) PIR JX0331, PC2194 (519 amino acids)
            Kawashima,H., Kusunose,E., Kikuta,Y., Kinoshita,H., Tanaka,S.,
            Yamamoto,S., Kishimoto,T. and Kusunose,M.
            Purification and cDNA cloning of human liver CYP4A fatty acid
            omega hydroxylase.
            J. Biochem. 116, 74-80 (1994)

CYP4A11     human kidney
            GenEMBL D13705 (1809bp)
            Kikuta,Y.
            unpublished (1994)
            P-450 HVK, fatty acid omega hydroxylase

CYP4A22       human
              GenEMBL AL390073.5, AF208532 AL135960 all genomic sequences
              new 4A11 like genomic sequence 95% identical to 4A11 
              three different gene sequences agree so this gene is different from 4A11
MSVSVLSPSRRLGGVSGILQVTSLLILLLLLIKAAQLYLHRQWLLKALQQFPCPPSHWLFGHIQE
FQHDQELQRIQERVKTFPSACPYWIWGGKVRVQLYDPDYMKVILGRS
DPKSHGSYKFLAPRI
GYGLLLLNGQTWFQHRRMLTPAFHNDILKPYVGLMADSVRVML
DKWEELLGQDSPLEVFQHVSLMTLDTIMKSAFSHQGSIQVDR
NSQSYIQAISDLNSLVFCCMRNAFHENDTIYSLTSAGRWTHRACQLAHQHT
DQVIQLRKAQLQKEGELEKIKRKRHLDFLDILLLAK
MENGSILSDKDLRAEVDTFMFEGHDTTASGISWILYALATHPKHQERCREEIHGLLGDGASITW
NHLDQMPYTTMCIKEALRLYPPVPGIGRELSTPVTFPDGRSLPKG
IMVLLSIYGLHHNPKVWPNLE
VFDPSRFAPGSAQHSHAFLPFSGGSR
NCIGKQFAMNQLKVARALTLLRFELLPDPTRIPIPMARLVLKSKNGIHLRLRRLPNPCEDKDQL*

Also AF208532
Homo sapiens fatty acid omega-hydroxylase CYP4A11 like gene, complete cds
CDS             join(2564..2758,5858..5999,6649..6693,7186..7313,
                     8347..8471,8840..8994,9435..9541,9628..9818,9915..10048,
                     10944..11008,11155..11231,13680..13875)

also AL135960 matches new 4A gene not 4A11 gene
Human DNA sequence from clone RP1-18D14 on chromosome 1 Contains genes for SIL (TAL1
 (SCL) interrupting locus ), TAL1 (T-cell acute lymphocytic leukemia 1), 17 KD membrane associated protein (DD96 protein), CYP4A11 -like gene
gi|6635875|emb|AL135960.1|AL135960[6635875]
CDS             complement(join(129154..129349,131798..131874,
                     132021..132085,132981..133147,133211..133401,
                     133488..133594,134035..134189,134558..134682,
                     135716..135843,136336..136380,137030..137171,
                     140271..140465))

cyp4a12     mouse
            GenEMBL X71479 (423bp) PIR S35610 (141 amino acids)
            Bell,D.R., Plant,N.J., Rider,C.G., Na,L., Brown,S., Ateitalla,I.,
            Acharya,S.K., Davies,M.H., Elias,E.E., Jenkins,N.A.,
            Gilbert,D.J., Copeland,N.G. and Elcombe,C.R.
            Species-specific induction of cytochrome P-450 4A RNAs: PCR 
            cloning of partial guinea-pig, human and mouse cDNAs.
            Biochem. J. 294, 173-180 (1993)
            PCR fragment

            GenEMBL Y10222 (exon 12)
            Heng,Y.M., Kuo,C.W.S., Jones,P.S., Savory,R., Shultz,R.M.,
            Tomlinson,S.R., Gray,T.J.B. and Bell,D.R.
            A novel murine P-450 gene, Cyp4a14, is part of a cluster of Cyp4a
            and Cyp4b, but not of CYP4F, genes in mouse and humans.
            Biochem. J. 325, 741-749 (1997)
            Chromosomal assignment of this gene is given.

Cyp4a12     mouse
            no accession number
            Jorge Capdevila
            submitted to nomenclature committee 12/4/96 fax
            complete sequence

CYP4A13     Cavia cobaya (guinea pig)
            GenEMBL X71481 (423bp)
            PIR S32714 (141 amino acids) PIR S35611 (141 amino acids)
            Bell,D.R., Plant,N.J., Rider,C.G., Na,L., Brown,S., Ateitalla,I.,
            Acharya,S.K., Davies,M.H., Elias,E.E., Jenkins,N.A.,
            Gilbert,D.J., Copeland,N.G. and Elcombe,C.R.
            Species-specific induction of cytochrome P-450 4A RNAs: PCR 
            cloning of partial guinea-pig, human and mouse cDNAs.
            Biochem. J. 294, 173-180 (1993)

Cyp4a14     mouse
            GenEMBL Y11639 to Y11642 exons 1-12
            Heng,Y.M., Kuo,C.W.S., Jones,P.S., Savory,R., Shultz,R.M.,
            Tomlinson,S.R., Gray,T.J.B. and Bell,D.R.
            A novel murine P-450 gene, Cyp4a14, is part of a cluster of Cyp4a
            and Cyp4b, but not of CYP4F, genes in mouse and humans.
            Biochem. J. 325, 741-749 (1997)

Cyp4a14     mouse
            no accession number
            Jorge Capdevila
            submitted to nomenclature committee
            complete sequence

CYP4A15     Koala 
            No accession number
            Ieva Stupans
            Submitted to nomenclature committee Oct. 20, 1999
            69% identical to human 4A11

CYP4A16     Felis cattus (domestic cat)
            GenEMBL U91787
            Gebremedhin,D.G., Lange,A.R., Narayanan,J., Aebly,M., Jacobs,E. and
            Harder,D.R.
            Cerebral vascular smooth muscle cells express cytochrome P450 4A2
            enzyme and produces 20-HETE which enhances L-type calcium current
            Unpublished

CYP4A17     hamster
            No accession number
            David Bell
            Submitted to nomenclature committee 12/1/99
            170 amino acid C-terminal fragment from I-helix to heme
            Clone name 4ai 90% to mouse Cyp4a10 95% to 4A19

CYP4A18     hamster
            No accession number
            David Bell
            Submitted to nomenclature committee 12/1/99
            170 amino acid C-terminal fragment from I-helix to heme
            Clone name 4aii 88% to rat CYP4A1 88% to 4A19

CYP4A19     hamster
            No accession number
            David Bell
            Submitted to nomenclature committee 12/1/99
            170 amino acid C-terminal fragment from I-helix to heme
            Clone name 4aiii 92% to rat CYP4A1 95% to 4A17

CYP4A20X    human 
            GenEMBL AJ131016 AC026935 161971-176942 
            Formerly named 4Z1 from a fragment renamed 4A20 then restored to 4Z1
            Full length seq now available on genomic DNA
            52% to 4A11 52% to 4X1 
            because the sequence is the same percent identity to
            4A11 and 4X1 and this is less than 55% 
            and the sequence does not cluster with CYP4As on a tree
            the earlier name of CYP4Z1 is being resurrected.
            This seems to be a more accurate name.
MEPSWLQELMAHPFLLLILLCMSLLLFQVIRLYQRRRWMIRALHLFPAPPAHWFYGHKE
FYPVKEFEVYHKLMEKYPCAVPLWVGPFTMFFSVHDPDYAKILLKRQDP
KSAVSHKILESWVGRGLVTLDGSKWKKHRQIVKPGFNISILKIFITMMSE
SVRMML
NKWEEHIAQNSRLELFQHVSLMTLDSIMKCAFSHQGSIQLDRS
SYLKAVFNLSKISNQRMNNFLHHNDLVFKFSSQGQIFSKFNQELHQFT
HLEKVIQDRKESLKDKLKQDTTQKRRWDFLDILLSAKV
ENTKDFSEADLQAEVKTFMFAGHDTTSSAISWILYCLAKYPEHQQRCRDEIRELLGDGSSITW
EHLSQMPYTTMCIKECLRLYAPVVNISRLLDKPITFPDGRSLPA
GITVFINIWALHHNPYFWEDPQV
FNPLRFSRENSEKIHPYAFIPFSAG
PRNCIGQHFAIIECKVAVALTLLRFKLAPDHSRPPQPVRQVVLKSKNGIHVFAKKV

CYP4A21     pig 
            GenEMBL AJ278474 
            Lundell K, Hansson R, Wikvall K.
            Cloning and expression of a pig liver taurochenodeoxycholic acid 
            6 alpha-hydroxylase (CYP4A21): a novel member of the CYP4A subfamily.
            J Biol Chem. 276, 9606-12 (2001)
            77% to rabbit 4A6
            submitted to nomenclature committee July 3, 2000
            This enzyme catalyzes a 6alpha-hydroxylation of chenodeoxycholic acid

CYP4A22       human
              GenEMBL AL390073.5, AF208532 AL135960 all genomic sequences
              new 4A11 like genomic sequence 95% identical to 4A11 
              three different gene sequences agree so this gene is different from 4A11
MSVSVLSPSRRLGGVSGILQVTSLLILLLLLIKAAQLYLHRQWLLKALQQFPCPPSHWLFGHIQE
FQHDQELQRIQERVKTFPSACPYWIWGGKVRVQLYDPDYMKVILGRS
DPKSHGSYKFLAPRI
GYGLLLLNGQTWFQHRRMLTPAFHNDILKPYVGLMADSVRVML
DKWEELLGQDSPLEVFQHVSLMTLDTIMKSAFSHQGSIQVDR
NSQSYIQAISDLNSLVFCCMRNAFHENDTIYSLTSAGRWTHRACQLAHQHT
DQVIQLRKAQLQKEGELEKIKRKRHLDFLDILLLAK
MENGSILSDKDLRAEVDTFMFEGHDTTASGISWILYALATHPKHQERCREEIHGLLGDGASITW
NHLDQMPYTTMCIKEALRLYPPVPGIGRELSTPVTFPDGRSLPKG
IMVLLSIYGLHHNPKVWPNLE
VFDPSRFAPGSAQHSHAFLPFSGGSR
NCIGKQFAMNQLKVARALTLLRFELLPDPTRIPIPMARLVLKSKNGIHLRLRRLPNPCEDKDQL*

Also AF208532
Homo sapiens fatty acid omega-hydroxylase CYP4A11 like gene, complete cds
CDS             join(2564..2758,5858..5999,6649..6693,7186..7313,
                     8347..8471,8840..8994,9435..9541,9628..9818,9915..10048,
                     10944..11008,11155..11231,13680..13875)

also AL135960 matches new 4A gene not 4A11 gene
Human DNA sequence from clone RP1-18D14 on chromosome 1 Contains genes for SIL (TAL1
 (SCL) interrupting locus ), TAL1 (T-cell acute lymphocytic leukemia 1), 17 KD membrane associated protein (DD96 protein), CYP4A11 -like gene
gi|6635875|emb|AL135960.1|AL135960[6635875]
CDS             complement(join(129154..129349,131798..131874,
                     132021..132085,132981..133147,133211..133401,
                     133488..133594,134035..134189,134558..134682,
                     135716..135843,136336..136380,137030..137171,
                     140271..140465))

CYP4A23     pig
            GenEMBL AF384031
            Van Es,A., Calleja,C. and Galtier,P.
            Cloning of a new member of the cyp4a subfamily in the pig renal
            cell line LLC-PK1
            Unpublished
            Note: CYP4A23, 24 and 25 are all on the border of being alleles of the same gene

CYP4A24     pig 
            GenEMBL AJ318096
            Kerstin Lundell 
            97% to pig 4A25, 97% to pig 4A23 AF384031, 90% to pig 4A21 AJ278474 
            77% to rabbit 4A6, 75% to human 4A11
            submitted to nomenclature committee June 12, 2001
            Note: CYP4A23, 24 and 25 are all on the border of being alleles of the same gene

CYP4A25     pig 
            GenEMBL AJ318097
            Kerstin Lundell 
            97% to pig 4A24, 96% to pig 4A23, 91% to pig 4A21, 78 % to rabbit 4a6, 
            75% to human 4A11
            submitted to nomenclature committee June 12, 2001
            Note: CYP4A23, 24 and 25 are all on the border of being alleles of the same gene

CYP4A26P    human
            AL731892.5 
            76% to 4A11 C-term
            SAWLRTCIGKKFAMNEL 135092
            135091 KVAMALTLLRFELLPDPTRIPVGIP*ILLKFKSGIHLHLRRV 134966

CYP4A27P    human
            AL731892.5 
            67% to 4A11 C-term
           168353 SAWLRNCIGKQFAMNELKVALTLLNFKLFSDPASIPILLPQMVLKFNNGIHLHVKRL 168183

Cyp4a28p    mouse
            AJ297131 
            lower case = 4a10, same contig as 4x1
4.112000001-113000000 from Ensembl mouse version 3 chr 4 add 112 million to numbers
943832 MSISALSPTRFLECVSGFLQVVSLIGLLLLLFKAAQHYLRRQWLLRALYQFPSPPSHWLFGH 943647 
939707 QFKEDEELRWVLRYVEKFPSAFLHWIWGN 939621 frameshift
939621 *VFIKIYDPDYMKVILGRA 939565                                   
939009 SDPKAGLYRFLTPWL 938965                                          
938111 GHGLFILNGHKWFQHRRMLTSAFHYDILKPYVGTMADSVQVIL 937983               
930960 DKWEQIACQDITLEIFHPISLMMLETIMKCAFSYQGSVQLDRS 930832               
930514 RSSQAYLQAVSDLNNLVTSRMTNVFLHNDIIYNLTSHSRRTNSACQIAHEHT 930359      
929925 DRVVKLRKAQLQDNKSMEKLRGKRCLDFLDILLLSRVSS 929809        
       DDGSSLSDKALRAEVDSLMFGDHDTPASGISWVFYALATHPNHQQRCTEEVQSLLGDGSPITW 929542 
       EHLHQMPYTTLCVKEAQRLYPTVPSVGRKLNTPVTFPDGRSLPKG 929424
(missing exon 10)
927997 VFDPSRFAMNSVQHSHAFLPFSGGS 927923                               
926387 RNCIGKQLAMNALKVAVALTLLRFELLPDPT*VPIPTHQLILKSTNGIHL 926238        

exact duplicate of exons 6-12
925899 RSSQAYLQAVSDLNNLVTSRMTNVFLHNDIIYNLTSHSRRTNSACQIAHEHT
       DRVVKLRKAQLQDNKSMEKLRGKRCLDFLDILLLSRVSS 925195
       DDGSSLSDKALRAEVDSLMFGDHDTPASGISWVFYALATHPNHQQRCTEEVQSLLGDGSPITW 924927 
       EHLHQMPYTTLCVKEAQRLYPTVPSVGRKLNTPVTFPDGRSLPKG 924809
923831 IESTVSCFIFSSAWKAFDKPE
923372 VFDPSRFAMNSVQHSHAFLPFSGGS 923298                               
922002 RNCIGKQLAMNALKVAVALTLLRFELLPDPT*VPIPTHQLILKSTNGIHLHLRKL 921838   

>Cyp4a29    mouse
4.113000001-114000000 chr 4 from mouse Ensembl version 3 add 113 million to numbers
91% to 4A28p
47095 MSISALSPIRFLECVSGFLQVIFLIGLLLLLFKAAQHYLRRQWLLRALYQFPSPPSHWLFGH 47280 
53123 QFKVDEELRWILRYLEKFPGAFLHWIWGSHVFIKVCDPDYMKVILGRA 53266          
53824 SDPKASLYRFLTPWL 53868                                           
54675 GHGLFFLNGDEWFQHRRLLTPAFHYDILKPYVGIMADSVQVML 54803
55849 EKWEQIACQDITLEIFHPISLMMLDTIMKYAFSYQGSVQLDRS 55977                 
56295 RSSQAYLQAVSDLNNLVTSRMKNVFLHNDIIYNLTSHSRRTNSACQIAHEHT 56450        
56880 DRVVKLRKAQLQDNKSMKKLRGKRCLDFLDILLLSRVSS 56996
57075 DDGSSLSDKALRAEVDSLMFGGHDTPASGISWVFYALATHPDHQQRCREEVQSLLGDGSPITW 57263 
57362 DHLHQMPYTTMCIKEALRLYPPIPSVGRKLSTPVTFPDGRSLPKG 57378
58567 IESTVSCFIFFSAWKAFDKPE
59045 VFDPSRFAMNSVQHSHAFLPFSGGS 59119                                 
60585 RNCIGKHLAMNVLKVAVALTLLRFELLPDPSRVPIPTQQLVLKSKNGIYLHLRKL 60749     

CYP4A       rabbit
            PIR PQ0048 (25 amino acids)
            Kikuta, Y., Kusunose, E., Okumoto, T., Kubota, I. and Kusunose,M.
            Purification and characterization of two forms of cytochrome
            P-450 with omega-hydroxylase activities toward
            prostaglandin A and fatty acids from rabbit liver
            microsomes.
            J. Biochem. 107, 280-286 (1990)
            This fragment has 2 differences with 4A7

4B Subfamily

CYP4B1      human
            GenEMBL NM_000779 J02871
            Nhamburo,P.T., Gonzalez,F.J., McBride,O.W., Gelboin,H.V. and
            Kimura,S.
            Identification of a new P450 expressed in human lung: Complete cDNA
            sequence, cDNA-directed expression and chromosome mapping
            Biochemistry 28, 8060-8066 (1989)

CYP4B1      human
            GenEMBL X16699
            Yokotani,N., Sogawa,K., Matsubara,S., Gotoh,O., Kusunose,E.,
            Kusunose,M. and Fujii-Kuriyama,Y.
            cDNA cloning of cytochrome P-450 related to P-450p-2 from the cDNA
            library of human placenta. Gene structure and expression
            Eur. J. Biochem. 187 (1), 23-29 (1990)

CYP4B1      rat 
            GenEMBL M29853
            Gasser,R. and Philpot,R.M.
            Primary structures of cytochrome P-450 isozyme 5 from rabbit and
            rat and regulation of species-dependent expression and induction in
            lung and liver: Identification of a new cytochrome P-450 gene
            subfamily IVB
            Mol. Pharmacol. 35, 617-625 (1989)

CYP4B1      rabbit 
            GenEMBL M29852
            Gasser,R. and Philpot,R.M.
            Primary structures of cytochrome P-450 isozyme 5 from rabbit and
            rat and regulation of species-dependent expression and induction in
            lung and liver: Identification of a new cytochrome P-450 gene
            subfamily IVB
            Mol. Pharmacol. 35, 617-625 (1989)

CYP4B1      rabbit
            GenEMBL AF176914
            Mastyugin,V., Aversa,E., Bonazzi,A., Vafaes,C., Mieyal,P. and
            Schwartzman,M.L.
            Hypoxia-induced production of 12-hydroxyeicosanoids in the corneal
            epithelium: involvement of a cytochrome P-4504B1 isoform
            J. Pharmacol. Exp. Ther. 289 (3), 1611-1619 (1999)

Cyp4b1      mouse
            GenEMBL D50834 (1871bp)
            Imaoka,S., Hiroi,T., Tamura,Y., Yamazaki,H., Shimada,T., Komori,M.,
            Degawa,M. and Funae,Y.
            Mutagenic activation of 3-methoxy-4-aminoazobenzene by mouse renal
            cytochrome P450, CYP4B1: Cloning and characterization of mouse
            Cyp4b1.
            Arch. Biochem. Biophys. 321, 255-262 (1995)

CYP4B1      wallaby
            No accession number
            Ross McKinnon
            Submitted to nomenclature committee Dec. 2, 2002
            Clone name W4B2
            73% to mouse Cyp4b1

CYP4B1      Bos taurus cattle
            GenEMBL AW347853
            Differs from 4B2 shown below
3 DGRSLPAGSLISLHIYALHRNSAVWPDPEVFDPLRFSLENMAGRHPFAFLPFSAGPRNC 179

CYP4B2      Capra hircus (goat)
            No accession number
            Garold Yost
            Submitted to nomenclature committee August 2002
            82% to human 4B1, 94% to bovine 4B2 (partial)

CYP4B2      Bos taurus cattle
            GenEMBL BF602740
            94% to goat 4B2 only 82% to human 4B1
3   AISWVLYCMSLYPEHQRRCREEIQEILGDRDTLKWDDLAEMTYLTMCIKESFRLYPPVPQ 182
183 VYRQLSQPVNFVDGRSLPEGSLISLHIYALHRNSTVWPDPEVFDPLRFSPENVARRHSFA 362
363 FIPFSAGPRNCIGQQFAMAEVKVVTALCLLRFEFAPDP 476

CYP4B3      wallaby
            No accession number
            Ross McKinnon
            Submitted to nomenclature committee Dec. 2, 2002
            Clone name W4B1
            69% to mouse Cyp4b1

4C Subfamily

CYP4C1      Blaberus discoidalis (cockroach)
            GenEMBL M63798 Swiss P29981 (511 amino acids)
            Bradfield,J.Y., Lee,Y.-H. and Keeley,L.L.
            Cytochrome P450 family 4 in a cockroach: Molecular cloning and
            regulation by hypertrehalosemic hormone
            Proc. Natl. Acad. Sci. U.S.A. 88, 4558-4562 (1991)

CYP4C2      Anopheles albimanus (mosquito)
            GenEMBL L38678 (379bp) PIR PC2257 (126 amino acids)
            Scott, J.A., Collins,F.H. and Feyereisen, R.
            Diversity of cytochrome P450 genes in the mosquito, Anopheles 
            albimanus.
            Biochem. Biophys. Res. Commun. 205, 1452-1459 (1994)

Cyp4c3      Drosophila melanogaster
            GenEMBL U34323(379bp)
            Dunkov,B.C., Rodriguez-Arniaz,R., Pittendrigh,B.,
            ffrench-Constant,R.H. and Feyereisen,R.
            Cytochrome P450 gene clusters in Drosophila melanogaster.
            Mol. Gen. Genet. 251, 290-297 (1996)

Cyp4c3      Drosophila melanogaster
            AC014779 73845-80196 also AC008219

CYP4C4      Diploptera punctata (Pacific beetle roach)
            GenEMBL AF071073
            Andersen,J.F. and Feyereisen,R.
            Cockroach fat body CYP4C4
            Unpublished

CYP4C5      Diploptera punctata (Pacific beetle roach)
            GenEMBL AF071074
            Andersen,J.F. and Feyereisen,R.
            Cockroach fat body CYP4C5
            Unpublished

CYP4C6      Diploptera punctata (Pacific beetle roach)
            GenEMBL AF071075
            Andersen,J.F. and Feyereisen,R.
            Cockroach fat body CYP4C6
            Unpublished

CYP4C7      Diploptera punctata (Pacific beetle roach)
            GenEMBL AF071072
            Andersen,J.F. and Feyereisen,R.
            CYP4C7, a P450 from cockroach corpora allata
            Unpublished

CYP4C8      Mastotermes darwiniensis (Australian termite)
            GenEMBL U77126(432bp)
            Falckh,P.H.J., Balcombe,W.M., Haritos,V.S. and Ahokas,J.T.
            Isolation, identification and sequence of a cytochrome P450 in an
            Australian termite, Mastotermes darwiniensis.
            unpublished (1996)
            note: 92 amino acid C-terminal fragment

CYP4C9      Coptotermes acinaciformis (termite)
            GenEMBL AF046008
            Patrick Falckh
            C4-8_9-A 

CYP4C10     Coptotermes acinaciformis (termite)
            GenEMBL AF046009
            Patrick Falckh
            C4-8_9-I 

CYP4C11     Mastotermes darwiniensis (Australian termite)
            no accession number
            Quoc Wu
            M4-150.4

CYP4C12     Mastotermes darwiniensis (Australian termite)
            no accession number
            Quoc Wu
            M4-100-8

CYP4C13     Mastotermes darwiniensis (Australian termite)
            no accession number
            Quoc Wu
            M4-240-2

CYP4C14     Coptotermes acinaciformis (termite)
            no accession number
            Quoc Wu
            4F-22_24

CYP4C15     Orconectes limosus (crayfish)
            GenEMBL AF091117
            Chantal Dauphin-Villemant
            submitted to nomenclature committee
            this is a tentative assignment, this sequence may require its own  
            subfamily
            50% identical to 4C1 over full length, 58% identical over C-terminal

CYP4C16     Penaeus setiferus (shrimp)
            GenEMBL AF072854
            Mark Snyder
            submitted to nomenclature committee

CYP4C17     Haliotis rufescens (red abalone)
            GenEMBL AF072853
            Mark Snyder
            submitted to nomenclature committee

CYP4C18     Homarus americanus (lobster)
            GenEMBL AF072852
            Mark Snyder
            submitted to nomenclature committee

CYP4C19     Lytechinus anamesis (sea urchin)
            GenEMBL AF072850
            Mark Snyder
            submitted to nomenclature committee
            first sequence from sea urchin

CYP4C20     Lytechinus anamesis (sea urchin)
            GenEMBL AF072851
            Mark Snyder
            submitted to nomenclature committee
            second sequence from sea urchin

CYP4C21     Blattella germanica (German cockroach)
            no accession number
            Jeff Scott
            submitted to nomenclature committee Dec. 30, 1998

CYP4C22v1   Culex pipiens pallens (mosquito)
            GenEMBL AF157093
            Zhu Changliang, Dept. of Parasitology, Nanjing University
            submitted to nomenclature committee 5/20/99
            PCR C-terminal fragment 150 amino acids
            clone name NYDR2 81% identical to 4C2 
            this sequence has one frameshift

CYP4C22v2  Culex pipiens
           No accession number
           Changliang Zhu
           Clone NYDS6 PCR fragment 97% identical to 4C22v1
           Submitted to nomenclature committee 9/30/99

CYP4C23    Culex pipiens pallens (mosquito)
           GenEMBL AF157090
           Zhu Changliang, Dept. of Parasitology, Nanjing University
           submitted to nomenclature committee 5/20/99
           PCR C-terminal fragment 167 amino acids
           clone name NYDS1 84% identical to AF117650 at C-terminal 44 amino acids 
           this sequence has numerous frameshifts and insertions/deletions it may
           be a pseudogene

CYP4C24    Orconectes limosus (crayfish)
           no accession number
           C. Dauphin-Villemant, M. Tom, M. Waterman and D. Bšcking
           submited to nomenclature committee 6/15/99

CYP4C25    Anopheles gambiae
           no accession number
           Hilary Ranson
           Submitted to nomenclature committee 8/9/99
           clone name P450Q 
           CYPb1x3 on chromosome 1
           91% identical to 4C2

CYP4C25     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPb1x3

CYP4C26    Anopheles gambiae
           no accession number
           Hilary Ranson
           Submitted to nomenclature committee 8/9/99
           clone name P450S
           CYPb1x1 on chromosome 1
           73% identical to 4C2

CYP4C26     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPb1x1

CYP4C27    Anopheles gambiae
           no accession number
           Hilary Ranson
           Submitted to nomenclature committee 8/9/99
           clone name P450I
           CYPo3r3 on chromosome 3
           58% identical to 4C6

CYP4C27     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPo3r3

CYP4C28    Anopheles gambiae
           no accession number
           Hilary Ranson
           Submitted to nomenclature committee 8/9/99
           clone name P450R
           CYPs3l1 on chromosome 3
           61% identical to 4C2

CYP4C28     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPs3l1

CYP4C29    Cherax quadricarinatus (crayfish)
           No accession number 
           Moshe Tom
           Submitted to nomenclature committee May 4, 2000
           Clusters with the other crayfish sequence 4C24 on trees

CYP4C30    Coscinasterias muricata Australian sea star (11 armed starfish)
           No accession number
           Karl Herron
           Submitted to nomenclature committee Oct. 9, 2001
           63% to 4C19 partial seq. from I-helix to heme

CYP4C31    Daphnia pulex (Daphnia, water flea)
           No accession number
           Dauphin-Villemant, C., David, J-P, Mesneau, A., Meyran, J-C.
           Submitted to nomenclature committee Feb. 27, 2002
           Clone name 2.5 203 aa
           65% to 4C24 crayfish 65% to 4C16 shrimp 69% to 4C18 lobster 
           68% to 4C17 abalone 82% to 4C33

CYP4C32    Daphnia pulex (Daphnia, water flea)
           No accession number
           Dauphin-Villemant, C., David, J-P, Mesneau, A., Meyran, J-C.
           Submitted to nomenclature committee Feb. 27, 2002
           Clone name 2.6 149 aa
           95% to 4C34

CYP4C33    Daphnia pulex (Daphnia, water flea)
           No accession number
           Dauphin-Villemant, C., David, J-P, Mesneau, A., Meyran, J-C.
           Submitted to nomenclature committee Feb. 27, 2002
           Clone name 2.14 200 aa
           62% to 4C24 crayfish 61% to 4C16 shrimp 66% to 4C18 lobster 
           66% to 4C17 abalone 82% to 4C31

CYP4C34    Daphnia pulex (Daphnia, water flea)
           No accession number
           Dauphin-Villemant, C., David, J-P, Mesneau, A., Meyran, J-C.
           Submitted to nomenclature committee Feb. 27, 2002
           Clone name 2.16 206 aa
           61% to 4C24 crayfish 63% to 4C16 shrimp 64% to 4C18 lobster 
           65% to 4C17 abalone 95% to 4C32

CYP4C35     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPo3r1

CYP4C36     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPo3r2

CYP4C37     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPb1x2

CYP4C38    Aedes aegypti (mosquito)
           No accession number
           David, J-P  and Dauphin-Villemant, C.
           I-helix to heme fragment
           Clone name 4T1.4

CYP4C39    Carcinus maenas (shore crab) 
           GenEMBL AY328467
           Kim Rewitz
           submitted to nomenclature committee 6/18/2003
           59.5% to 4C15

4D Subfamily

Cyp4d1      Drosophila melanogaster
            GenEMBL X67645 (1675bp) Swiss P33269 (511 amino acids)
            PIR S25707 (511 amino acids)
            Gandhi,R., Varak,E. and Goldberg,M.L. 
            Molecular analysis of a cytochrome P450 gene of family 4 on the
            Drosophia X chromosome.
            DNA Cell Biol. 11, 397-404 (1992)
            note: X67645 has a probable frameshifts at the C-terminal
            see Z98269 and AF016992 to AF017004 for correct end

Cyp4d1      Drosophila melanogaster
            GenEMBL AC017770 49988-51720
            Alternative splice AC017770 48817-51720

Cyp4d2      Drosophila melanogaster
            GenEMBL Z23005 (1481bp) X75955 (1883bp) PIR S41192 (496 amino acids)
            Frolov,M.V. and Alatortsev,V.E.
            Cluster of cytochrome P450 genes on the X-chromosome of
            Drosophila melanogaster
            DNA Cell Biol. 13, 663-668 (1994)
            probable frameshift at C-terminal
            see Al009194 and AF017006 to AF017019 for correct end

Cyp4d2      Drosophila melanogaster
            GenEMBL AC017770 comp(32267-34028)

CYP4D3      Musca domestica (house fly)
            Rene Feyereisen
            submitted to nomenclature committee

CYP4D4      Musca domestica (house fly)
            Rene Feyereisen
            submitted to nomenclature committee

CYP4D5      Anopheles albimanus (mosquito)
            GenEMBL L38679 (382bp) PIR PC2270 (127 amino acids)
            Scott, J.A., Collins,F.H. and Feyereisen, R.
            Diversity of cytochrome P450 genes in the mosquito, Anopheles 
            albimanus.
            Biochem. Biophys. Res. Commun. 205, 1452-1459 (1994)

CYP4D6      Anopheles albimanus (mosquito)
            GenEMBL L38680 (446bp) PIR PC2271 (127 amino acids)
            Scott, J.A., Collins,F.H. and Feyereisen, R.
            Diversity of cytochrome P450 genes in the mosquito, Anopheles 
            albimanus.
            Biochem. Biophys. Res. Commun. 205, 1452-1459 (1994)

CYP4D7      Anopheles albimanus (mosquito)
            GenEMBL L38681 (447bp) PIR PC2272 (127 amino acids)
            Scott, J.A., Collins,F.H. and Feyereisen, R.
            Diversity of cytochrome P450 genes in the mosquito, Anopheles 
            albimanus.
            Biochem. Biophys. Res. Commun. 205, 1452-1459 (1994)

Cyp4d8      Drosophila melanogaster
            GenEMBL U34329(385bp)
            Dunkov,B.C., Rodriguez-Arniaz,R., Pittendrigh,B.,
            ffrench-Constant,R.H. and Feyereisen,R.
            Cytochrome P450 gene clusters in Drosophila melanogaster.
            Mol. Gen. Genet. 251, 290-297 (1996)
            Dro-G453 (formerly called 4N3)

Cyp4d8      Drosophila melanogaster
            GenEMBL AC017388 comp(37292-39859) 
            also AC010557 AC010112 AC010113

CYP4D9      Musca domestica
            Rene Feyereisen
            no accession number
            6-full
            submitted to nomenclature committee

CYP4D10     Drosophila mettleri
            GenEMBL U91634
            Jim Fogleman
            full length sequence  288mt
            submitted to nomenclature committee (one of 109 sequences)

CYP4D11     Ceratitis capitata (Mediterranean fruit fly)
            Phil Danielson
            Submitted to nomenclature committee
            MD60 PCR of C-terminal

CYP4D12     Ceratitis capitata (Mediterranean fruit fly)
            Phil Danielson
            Submitted to nomenclature committee
            MD5 PCR of C-terminal

CYP4D13     Ceratitis capitata (Mediterranean fruit fly)
            Phil Danielson
            Submitted to nomenclature committee
            MD84 PCR of C-terminal

Cyp4d14     Drosophila melanogaster
            GenEMBL AL009194 comp(10685-12421)
            also submitted by Michael Goldberg
            55% identical to Cyp4d2

Cyp4d14     Drosophila melanogaster
            GenEMBL AC017770 35699-37438

CYP4D15     Anopheles gambiae
            no accession number
            Hilary Ranson
            Submitted to nomenclature committee 8/9/99
            clone name P450E
            77% identical to 4D6

CYP4D15     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPe2r3

CYP4D16    Anopheles gambiae
           no accession number
           Hilary Ranson
           Submitted to nomenclature committee 8/9/99
           clone name P450F
           84% identical to 4D6

CYP4D16     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPe2r5

CYP4D17    Anopheles gambiae
           no accession number
           Hilary Ranson
           Submitted to nomenclature committee 8/9/99
           clone name P450H
           79% identical to 4D5

CYP4D17     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPe2r4

CYP4D18v1  Culex pipiens
           GenEMBL AF190775
           Changliang Zhu
           Clone NYDS2 PCR fragment 95% identical to 4D18v2, 66% to 4D6
           Submitted to nomenclature committee 9/30/99

CYP4D18v2  Culex pipiens
           No accession number
           Changliang Zhu
           Clone NYDR16 PCR fragment 95% identical to 4D18v1
           Submitted to nomenclature committee 9/30/99

CYP4D19v1  Culex pipiens
           No accession number
           Changliang Zhu
           Clone NYDR11 PCR fragment 3 diffs with 4D19v2
           Submitted to nomenclature committee 9/30/99

CYP4D19v2  Culex pipiens
           No accession number
           Changliang Zhu
           Clone NYDR14 PCR fragment 3 diffs with 4D19v1
           Submitted to nomenclature committee 9/30/99

CYP4D19v3  Culex pipiens
           GenEMBL AF190777
           Changliang Zhu
           Clone NYDS4 PCR fragment 95% identical to 4D19v2, 54% to 4D5
           Submitted to nomenclature committee 9/30/99

Cyp4d20    Drosophila melanogaster
           GenEMBL AC019509 comp(19731-21636)

Cyp4d21    Drosophila melanogaster
           GenEMBL AC020004 55466-57280

CYP4D22    Anopheles gambiae (malaria mosquito)
           no accession number
           Hilary Ranson
           Submitted to nomenclature committee 8/9/99
           clone name P450O
           50% identical to 4H5, 4H7 
           note the 4H subfamily might really be considered two differernt 
           subfamilies 4H1,2,3,4,9 in one group and 4H5,6,7,8 in another.  
           4H20 falls between these. Revised seq now groups with CYP4Ds
           formerly CYP4H20

CYP4D22     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPe2r6

CYP4D23v1  Aedes aegypti (mosquito)
           No accession number
           David, J-P  and Dauphin-Villemant, C.
           I-helix to heme fragment
           Clone name 4T2.8

CYP4D23v2  Aedes aegypti (mosquito)
           No accession number
           David, J-P  and Dauphin-Villemant, C.
           I-helix to heme fragment
           Clone name 4T1.3

CYP4D23v3  Aedes aegypti (mosquito)
           No accession number
           David, J-P  and Dauphin-Villemant, C.
           I-helix to heme fragment
           Clone name 4T1.1

CYP4D24    Aedes aegypti (mosquito)
           No accession number
           David, J-P  and Dauphin-Villemant, C.
           I-helix to heme fragment
           Clone name 4T2.6

CYP4D or 4AR   Aedes aegypti (mosquito)
           No accession number
           David, J-P  and Dauphin-Villemant, C.
           I-helix to heme fragment
           Clone name 4I1.3

CYP4D or 4AR   Aedes aegypti (mosquito)
           No accession number
           David, J-P  and Dauphin-Villemant, C.
           I-helix to heme fragment
           Clone name 4T1.6

4E Subfamily

Cyp4e1      Drosophila melanogaster
            GenEMBL AC020402 44580-46698
            CYP4E1 HAS INSERT AT C-TERM VSKDGYISTTLGLQPAEKKSRDAHNHK

Cyp4e2      Drosophila melanogaster
            no accession number 
            Amichot, M., Brun, A., Cuany, A., Helvig, C., Salaun, J. P., Durst, F.
            and Berge, J. B.
            Expression study of CYP genes in drosophila strains
            resistant or sensitive to insecticides. In: Cytochrome P450 
            Lechner M.C. ed. John Libbey Eurotext, Paris.pp. 689-692 (1994)
            (PCR fragment) 144 amino acid region 

Cyp4e2      Drosophila melanogaster
            GenEMBL X86076(1408bp)
            Amichot,M., Brun,A., Cuany,A., LeMouel,T. and Berge,J.
            Cloning and expression study of CYP4E2 a novel P450 gene in
            drosophila.
            unpublished (1995)

Cyp4e2      Drosophila melanogaster
            GenEMBL U56957(1458bp) U34332(373bp)
            Dunkov,B.C., Rodriguez-Arniaz,R., Pittendrigh,B.,
            ffrench-Constant,R.H. and Feyereisen,R.
            Cytochrome P450 gene clusters in Drosophila melanogaster.
            Mol. Gen. Genet. 251, 290-297 (1996)

Cyp4e2      Drosophila melanogaster
            GenEMBL AC020402 41848-44099

Cyp4e3      Drosophila melanogaster
            GenEMBL U34330(370bp)
            Dunkov, B.C., Rodriguez-Arnaiz, R., Pittendrigh, B., 
            ffrench-Constant, R.H. and Feyereisen,R.
            Cytochrome P450 gene clusters in Drosophila melanogaster.
            Mol. Gen. Genetics 251, 290-297 (1996)
            DroG4-2

Cyp4e3      Drosophila melanogaster
            GenEMBL AC020324 73117-75125

Cyp4e4x     Drosophila melanogaster
            GenEMBL U34331(379bp)
            Dunkov,B.C., Rodriguez-Arniaz,R., Pittendrigh,B.,
            ffrench-Constant,R.H. and Feyereisen,R.
            Cytochrome P450 gene clusters in Drosophila melanogaster.
            Mol. Gen. Genet. 251, 290-297 (1996)
            formerly called 4N4
            note: this sequence is pretty far out in the 4E subfamily 
            it has been reassigned as Cyp4ae1

CYP4E5      Drosophila mettleri
            GenEMBL U78486
            Phil Danielson
            Mt1

CYP4E6      Ceratitis capitata (Mediterranean fruit fly)
            Phil Danielson
            Submitted to nomenclature committee
            MD21 PCR of C-terminal

CYP4E7     Musca domestica (house fly)
           no accession number (530 amino acids)
           Claus Tittiger and Gary Blomquist
           submitted to nomenclature committee

4F Subfamily

CYP4F1      rat
            GenEMBL S53039 M94548 (1977bp) Swiss P33274 (524 amino acids)
            PIR S29723 (524 amino acids) 
            Chen,L. and Hardwick,J.P.
            Identification of a new P450 subfamily, CYP4F1, expressed
            in rat hepatic tumors.
            Arch. Biochem. Biophys. 300, 18-23 (1993)

CYP4F2      human
            GenEMBL U02388 (2368bp)
            Chen,L. and Hardwick,J.P. 
            The human liver CYP4F2 cDNA sequence and expression in
            baculovirus-infected cells.
            unpublished (1993)

CYP4F2      human
            GenEMBL D26480 (2227bp)
            Kikuta,Y., Kusunose,E., Kondo,T., Yamamoto,S., Kinoshita,H. and
            Kusunose,M.
            Cloning and expression of a novel form of leukotriene B4
            omega-hydroxylase from human liver
            FEBS Lett. 348, 70-74 (1994)

CYP4F3      human
            GenEMBL D12620 (2073bp) D12621 (1185bp) S63604 (2073bp)
            Swiss Q08477 (520 amino acids)
            Kikuta,Y., Kusunose,E., Endo,K., Yamamoto,S., Sogawa,K,
            Fujii-Kuriyama,Y. and Kusunose,M.
            A novel form of cytochrome P-450 family 4 in human
            polymorphonuclear leukocytes. cDNA cloning and expression of
            leukotriene B4 omega-hydroxylase.
            J. Biol. Chem. 268, 9376-9380 (1993)
            Note: three overlapping clones F-22, A-4 and M-6 are identical.
            Clone S-8 is different at 4 nucleotides and one amino acid.

CYP4F3v2    human
            GenEMBL AF054821
            Kanga Morgan
            alternative splice variant of 4F3 with a cryptic exon at amino acids 
            67-114 this exon resembles the region in 4F2.

CYP4F3      human
            GenEMBL AD000685 12 exons from 26660-44629

CYP4F4      rat
            GenEMBL U39206
            Kawashima, H. and Strobel, H.W. (1995)
            BBRC 217, 1137-1144

CYP4F5      rat
            GenEMBL U39207
            Kawashima, H. and Strobel, H.W. (1995)
            BBRC 217, 1137-1144

CYP4F6      rat
            GenEMBL U39208
            Kawashima, H. and Strobel, H.W. (1995)
            BBRC 217, 1137-1144

CYP4F7      Dicentrarchus labrax (European sea bass)
            GenEMBL AF045925
            Sabourault C, Amichot M, Pauron D, Berge JB, Lafaurie M, Girard JP
            Cloning of CYP4F7, a kidney-specific P450 in the sea bass Dicentrarchus  
            labrax.
            Biochem Biophys Res Commun 258, 155-61 1999
            62% identical to human 4F3
            authors name CYP4Dl2

Query:     1 GHDTTASGLSWVLYHLAKHPEYQERCRQEVQELLKDREPKEIEWDDLAQLPFLTMCIKES 60
             GHDTTAS + W LY+LA+H +YQE+CRQEV EL++ R+  EIEW+DL+ LPF TMCI+ES
Sbjct:     1 GHDTTASAICWTLYNLARHDQYQEKCRQEVMELMEGRDRHEIEWEDLSNLPFTTMCIRES 180

Query:    61 LRLHPPVPAVSRCCTQDIVLPDGRVIPKGIICLISVFGTHHNPAVWPDPEVYDPFRFDPK 120
             LRLH PV AV+R  TQD+ LP    +PKG ICL+S++GTHHNP VW +P  + P RFDP 
Sbjct:   181 LRLHSPVQAVTRKYTQDMTLPGDCTVPKGAICLVSIYGTHHNPTVWTNPHEFHPLRFDPT 360

Query:   121 NIKERSPLAFIPFSAGPRNCIGQAFAMAE 149
             N +  +  AFIPFS+GPRNCIGQ FA+AE
Sbjct:   361 NKEGLAAHAFIPFSSGPRNCIGQKFALAE 447

Sequence of reported protein may have 2 frameshifts at the end 
See alignment below to human 4F3 as query

Possible corrected sequence

GHDTTASAICWTLYNLARHDQYQEKCRQEVMELMEGRDRHEIEWEDLSNLPFTTMCIRES
LRLHSPVQAVTRKYTQDMTLPGDCTVPKGAICLVSIYGTHHNPTVWTNPHEFHPLRFDPT
NKEGLAAHAFIPFSSGPRNCIGQKFALAELRVVVALTLLRFRLTLGVNPEVGPSSGEVRR
LPQLVLRAEGGMWLQVEPL

 Score = 85 (29.9 bits), Expect = 6.3e-62, Sum P(2) = 6.3e-62
 Identities = 23/59 (38%), Positives = 31/59 (52%), Frame = +2

Query:   132 PFSAGPRNCIGQAFAMAEMKVVLGLTLLAFRVL--------PDHTEPRRKPELVLRAEG 182
             PF   P   + ++     ++VV+ LTLL FR+         P   E RR P+LVLRAEG
Sbjct:   395 PFPQAPGTALVRSSPWQRLRVVVALTLLRFRLTLGVNPEVGPSSGEVRRLPQLVLRAEG 571

 Score = 54 (19.0 bits), Expect = 1.2e-58, Sum P(2) = 1.2e-58
 Identities = 10/18 (55%), Positives = 12/18 (66%), Frame = +3

Query:   174 PELVLRAEGGLWLRVEPL 191
             P L     GG+WL+VEPL
Sbjct:   546 PSLSCVQRGGMWLQVEPL 599

CYP4F8      human
            GenEMBL AD000685 13 exons from 862-14599
            GenEMBL AF133298
            Bylund, J., Finnstršm, N. and Oliw, E. H. (1999) Gene Expression of a 
            Novel Cytochrome P450 of the CYP4F subfamily in Human Seminal Vesicles. 
            Biochem. Biophys. Res. Commun. 261, 169-174.
            submitted to nomenclature committee 10/30/98
            81.2% identity with CYP4F2 and 76.7% with CYP4F3.
            EST AI401776, AI349730

CYP4F9P     HUMAN
            GenEMBL AC004609 complement(13903-33104 region)
            pseudogene N-terminal not on this cosmid, may be on adjacent cosmid
            missing 71 amino acids at N-terminal
            69% identical to 4F3 67% identical to 4F8
EGMRVLTQLVATYPQGFKIWMNPITPIIRLCHPNIIWSVINASATIAPKD
EAFYKFLKPWLGDGLLVNASDKWSCHRQMLMPAFHFNMLKPYMKFFTDSV
NIMHAKWQLLASGGSAHLDMFEHTSLMTLDSVQKCVFSFDSHCQEKPSQYIATI
LELSSLFSXXXXXXXLCMDFLYYLIPSGWRFRRACCLVHDFTEAIIQEQRH
TLTSQGVDYFHEVKAKSKTLDFTDVLLLSKXXXXXXXXXXXXXXXXXXXXXX
GHNTTASGLSWVLYYLARHPEYQEHCWQEVQKLLKDHEPKEIEX
DDLAQLPFLTMYIKDSLWLHPPVPVISRCCTQDIVLPGG*VIPKGIVCLF
SNFETHHNPTVWLDPEVYDPFRFDPENSKERSPLAFIPFSAGSX
NCIGQAFAMAEMKVVLALTLLCFRVCPDHMEPRRKPEVIMYAEGGLWLWVKPLS

CYP4F10P   human
           GenEMBL AD000685 exons 3-6 (cosmid ends in middle of exon 6) 
           49350 to end of cosmid.  Cannot recognize exons 1 and 2 after 4F3 gene 
           ends at 44623 and before exon 3 starts at 49350.  May be a pseudogene 
           (No ESTs)
SATIAPKDKVFYSFLKPWLG
DGFLLSAGDKWSCHRGMLMPAFHFNILKPYMKIFDESVNIMH
AKWHLLTLEHNACLDMFEHMNLMTLDSLQKCVFSFDSQCQE
KPSEYIASVLELSSLVAKRNQQ

CYP4F11    human
           GenEMBL AC005336 cosmid F20129 end of cosmid
           ESTs T56269 and T56204 opposite ends of clone yb89b03
           ESTs T69576 and T69645 opposite ends of clone yc44c03
           EST AA991369 and EST W23003
           N-terminal up though C helix and C-terminal from I helix to end is 
           Present middle region is not present.  The rest of this gene should be 
           on cosmid R28342 this is not in the database yet. About 22 amino acids 
           missing at N-terminal
N-terminal is on AC011517 rest is on AC020950
MPQLSLSWLGLGPVAASPWLLLLLVGGSWLLARVLAWTYTFYDNCRRLQC
FPQPPKQNWFWGHQGLVTPTEEGMKTLTQLVTTYPQGFKLWLGPTFPLLI
LCHPDIIRPITSASAAVAPKDMIFYGFLKPWLGDGLLLSGGDKWSRHRRML
TPAFHFNLKPYMKIFNKSVNIMHDKWQRLASEGSARLDMFEHISLMTLDS
LQKCVFSFESNCQEKPSEYIAAILELSAFVEKRNQQILLHTDFLYYLTPDGQR
FRRACHLVHDFTDAVIQERRRTLPTQGIDDFLKNKAKSKTLDFIDVLLLSKD
EDGKELSDEDIRAEADTFMFEGHDTTASGLSWVLYHLAKHPEYQEQCRQEV
QELLKDREPIEIEWDDLAQLPFLTMCIKESLRLHPPVPVISRCCTQDFVLPDG
RVIPKGIVCLINIIGIHYNPTVWPDPEVYDPFRFNQENIKERSPLAFIPFSAGP
RNCIGQAFAMAEMKVVLALTLLHFRILPTHIEPRRKPELILRAEGGLWLRVEPLGANSQ

CYP4F12    human
           GenEMBL AC004523  missing N-terminal
           UNIGENE entry Hs.110130 (12 ESTs)
           UNIGENE Hs.168862 R08175 and R08229 (opposite end)
           Complete cDNA sequence submitted by Johan Bylund June 16, 2000
mRNA for cytochrome P450, complete cds. AB035130
MSLLSLPWLGLRPVAMSPWLLLLLVVGSWLLARILAWTYAFYNN
CRRLQCFPQPPKRNWFWGHLGL
ITPTEEGLKDSTQMSATYSQGFTVWLGPIIPFIVLC
HPDTIRSITNASAAIAPKDNLFIRFLKPWLGEGILLSGGDKWSRHRRMLTPAFHFNIL
KSYITIFNKSANIMLDKWQHLASEGSSRLDMFEHISLMTLDSLQKCIFSFDSHCQERP
SEYIATILELSALVEKRSQHILQHMDFLYYLSHDGRRFHRACRLVHDFTDAVIRERRR
TLPTQGIDDFFKDKAKSKTLDFIDVLLLSKDEDGKALSDEDIRAEADTFMFGGHDTTA
SGLSWVLYNLARHPEYQERCRQEVQELLKDRDPKEIEWDDLAQLPFLTMCVKESLRLH
PPAPFISRCCTQDIVLPDGRVIPKGITCLIDIIGVHHNPTVWPDPEVYDPFRFDPENS
KGRSPLAFIPFSAGPRNCIGQAFAMAEMKVVLALMLLHFRFLPDHTEPRRKLELIMRA
EGGLWLRVEPLNVGLQ

Cyp4f13    mouse
           GenEMBL ESTs AI304244 ui64h08.y1, AA277579 va77c04.r1
           AA238458 my34d11.r1, AA277631 va81d08.r1
           AI265156 uj03h07.y1, AI120320 ub69c04.r1
           AI327511 mb31b06.y1, AA245188 mx09e08.r1
           AA799238 vx69h12.r1, AA797204 vw22d08.r1
           AI196488 ui64h08.x1, AI196852 ui67e09.x1
           AA511038 vh55g04.r1,AI323999 mb31b06.x1
           W64560   md70f05.r1, AI121221 ud70e01.x1
           Unigene Mm.22045 
           89% identical to rat CYP4F6 probable ortholog
CONSENSUS
MLQLCLSWLGMGSLTASPWHLLLLGGASWILARILAWIYAFYDNCSRLRCFPQPPKPSWF
WGHLALMKNNEESMQFITHLGHDFHDVHLSWVGPVYPILRLVHPNFIAPLLQASAAVAPKEMTLYGFLKP 
WLGDGLLMSAGDKWSHHRRLLTPAFHFDILKSYVKIFNKSVNIMHAKWQCLASKGTSRLD 
MFEHISLMTLDSLQKCIFSVDSNCQESDSKYIAAILELSSLVVKRHRQP 
FLYLDLLYYLTADGRRFRKACDLVHNFTDAVIKERRSTLNTQGVEFLKAKAKTKTLD 
FIDVLLMAEDEHGKGLSNEDIRAEADTFMFGGHDTTTSALSWILYNXXXXXXXXXXXXX 
EVQELLRDRDSEEIEWDDLAQLPFLTMCIKESLRLHPPVLLISRCCTQDVLLPDGRAI
PKGNICVISIFGVHHNPSVWPDPEVYNPFRFDPENPQKRSPLAFIPFSAGTRNCIGQTFA
MSEIKVALALTLLRFRILPDDKEPRRKPELILRAEGGLWLRVEPLSAGAQ* 

Cyp4f14    mouse
           GenEMBL ESTs 
AI098768 ue38c01.y1 
AI047403 ud65h03.y1 
AI099192 ue40b11.y1
AA237519 mx10h12.r1 
AA793220 vl85g08.r1 
AA869706 vq44d03.r1
AI118365 ue40b11.x1 chimeric clone contains a-L-Fucosidase
AA880205 vx39b01.r1 

consensus N-terminal to 370
MSQLSLSWLGLGPEVAFPWKTLLLLGASWILARILIQIYAAYRNYRHLHGFPQPPKRNWL 
MGHVGMVTPTEQGLKELTRLVGTYPQGFLMWIGPMVPVITLCHSDIVRSILNASAAVALK 
DVIFYSILKPWLGDGLLVSAGDKWSRHRRMLTPAFHFNILKPYVKIFNDSTNIMHAKWQR 
VISDGSAPLDMFEHVSLMTLDSLQKCVVFSFDSNCQEKSSEYIAAILELSALVAK 
KDQQPLMFMDLLYNLTPDGMRFRKACNVVHEFTDAVIRERHRTLPDQGLDDFLKSKAKSKTLD 
FIDVLLLSKDEDGKELSDEDIRAEADTFMFEGHDTTASGLSWILYNLARHPEYQERCRQE 
VQELLRGREPEE 

gap of 69 amino acids

Cyp4f14 Cterminal 439-524
AI046446 ud65h03.x1 C-term 
AA274495 vb07d01.r1 C-term
AI121221 ud70e01.x1 C-term
consensus
VYDXFRFDPENIKDSSPLAFIPFSAGPRNCIGQTFAMSEMKVALALTLLRFRLLPDDKEPRRQPELILRAEGGLWLRVE
PLSAGAH*

UNIGENE entry Mm.10976 (C-terminal part only)
92% identical to rat CYP4F1 probable ortholog

Cyp4f15     mouse
           GenEMBL ESTs
AI315840 uj47c01.y1
AI046615 ud70e01.y1 
AI048594 ud61g02.y1 this clone skips over exons
MPQLDLSWLGLRLEASSPWLLLLLIGASWLLARVLTQTYIFYRTYHHLCDFPQPPKWNWF
LGHLGMITPTEHGLKEVTNLVATYPQGFMTWLGPIIPIITLCHPDIIRSVLNASASVALK
EVVFYSFLKPWLGDGLLLSDGDKWSSHRRMLTPAFHFNILKPYVKIFNDSTNIMHAKWQH
LALGGSARLDVFENISLMTLD

AI048594 ud61g02.y1 407-480
AI121221 ud70e01.x1 C-term this fragment also is an exact match to 4f13 and 4f14
There may have been gene conversion at the C-terminal of these three genes.
LLPDGRVIPKGVICIINIFATHHNPTVWPDPEVYDPFRFDPENIKDRSPLAFIPFSAGPR 
NCIGQTFAMNEMXVXXXXXXXXXXXXPDDKEPRRKPELILRAEGGLWLRVEPLSTQ

No UNIGENE entry for these ESTs
92% identical to rat CYP4F4 probable ortholog

Cyp4f16  mouse
         GenEMBL ESTs AA059667 mj77a08.r1 
         AA863889 vx15d11.r1, AA673923 vo83a09.r1
         AA798644 vw34b12.r1, AA896484 vx63d12.r1
MLRLSVSGLDLGSVVTSSWHLLLLGVASWILARILAWTYSFYENCSRLSCFPQPPKKNWF
SGHLGMIQSNEEGMQLVTEMGQTFQDVHLFWLGPVIPVLRIVDPAFVAPLLQAPALVAPKDMTFLRFLKPWL 
GDGLFLSSGDKWSRHRRLLTPAFHFDILKPYVKIFNQSVNIMHAKWKHLSSEGSARLEMF 
EHISLMTLDSLQKCLFGFDSNCQESPSEYISAILELSSXXXXXXXXXXXXXXX 

Cyp4f fragment a 93% identical to 4F5 88% to 4F6, 86% to 4F1 83% to 4F4
This fragment belongs to Cyp4f16
AA671839 vl02c09.r1
AA755081 vu02b08.r1 
LYYHTADGRRFRKACDLVHNFTDAVIRERRHTLSSQNHDEFLKSKTKSKTLDFIDVL
LLAKDEHGKELSDEDIRAEADTFMFGGHDTTASALSWILYNLARHPEYQERCRQRVQE
LLRDREPEEIEWDDLAQLPFLTMCIKESLRLHSPVIDXXXXXXX

Cyp4f fragment c 91% identical to 4F6, 86% to 4F1 and 4F5, 85% to 4F4
This fragment is part of Cyp4f16
AA688499 vq53e04.r1
AA791143 vv91d10.r1
AI036012 vz68a09.r1
AA794692 vu63c06.r1
DIVLXXXXXXPKGNICVISIFGIHHNPSVWPDPEXXX
PFRFDPENPQKRSPLAFIPFSAGPRNCIGQTFAMSEM
KVALALTLLRFRILPDDKEPRRKPEIILRAEGGLWLRVEPLSKGAQ*

92% identical to rat CYP4F5 probable ortholog

Cyp4f17   mouse
          GenEMBL ESTs AA387122 vc22e06.r1, AA386914 vc23b12.r1
          AA387149 vc22f06.r1
MLQLGLSWLGLGPGAAFPWQLLQLVGASLFLARILTWICAFYDNYCRLRCFPEPPSRHWF 
WGHMSMVKNNEEGLQLLTERSHQFHDVHLCWIGPFYPILRLIHPKFIGSILQASAAVAPK 
EMIFYGFLKPWLGDGLLVSAGEKWSRH 
66% to CYP4F6 but 81% identical to a rat EST AI030199
The rat homolog to this gene has not been cloned yet.

Cyp4f18   frag.b      mouse
          GenEMBL ESTs AA716998 vu61b04.r1 AI006742 ua82h10.r1
frag. d AI006742 ua82h10.r1
PKRNWILGHLGLIQSSEEGLLYIQSLVRTFRDACCWWVGPLHPVIRIFHPAFIKXVVLA
frag. b AA716998 vu61b04.r1
LLSKDEHGKALSDEDIRAEADTFMFGGHDTTASGLSWILYNLARHPEYQERCRQEVRD 
LLRDREPEEIEWDDLAQLPFLTMCIKESLRLHPPVTAISRCCTQDIVLPDGRVIPKGVIS 
RISIFGTHHNPAVWPDPEV 
Cyp4f18 fragments b and d 90% identical to 4F6, 89% to 4F4, 88% to 4F5, 87% to 4F1
complete sequence known but confidential (Henry Strobel)

CYP4F19     rat
            GenEMBL AI030199 
            probable rat ortholog to 4f17 81% identical
            opposite end of this clone = BF554573 N-terminal
4f17     1 MLQLGLSWLGLGPGAAFPWQLLQLVGASLFLARILTWICAFYDNYCRLRCFPEPPS 56
           MLQL LS LG GP    PWQLL +VG SL LARIL WI AFYDNYCRLRCFP+PPS
4f19     1 MLQLSLSCLGRGPVTVSPWQLLLVVGTSLLLARILGWISAFYDNYCRLRCFPQPPS 56

4f17 53 EPPSRHWFWGHMSMVKNNEEGLQLLTERSHQFHDVHLCWIGPFYPILRLIHPKFIGSILQA 113
        +P SRHWFWGH+++VKNNEEGLQLL E SHQF D+HLCWIG FYPILRLIHPKFIG ILQA
4F19 53 QPLSRHWFWGHLNLVKNNEEGLQLLAEMSHQFQDIHLCWIGIFYPILRLIHPKFIGPILQA 113
          * one amino acid difference in overlap

sequence = MLQLSLSCLGRGPVTVSPWQLLLVVGTSLLLARILGWISAFYDNYCRLRCFPQPPS
           RHWFWGHLNLVKNNEEGLQLLAEMSHQFQDIHLCWIGIFYPILRLIHPKFIGPILQA

Cacgaggcattaacagagaagagcggaagtctctgggactcctgagaaaagg  (start met)
atgctacagctgagcctg tcctgcctgg gccgggggcc agtgactgtc tccccatggc agcttctgct
ggtggttggg acctccttgc ttctggctcg aattctgggc tggatcagtg ctttctatga
caactactgc cgtctccgct gcttccctca gcccccttca
CGG CAC TGG TTT TGG GGT CAC TTG AAC TTG GTC AAG AAC AAT GAA GAA GGC CTG CAG TTG TTG GCT GAA ATG AGC CAC CAG TTC CAG GAT ATC CAC CTC TGT TGG ATT GGG ATC TTC TAC CCC ATC CTG AGG CTT ATC CAC CCC AAG TTT ATT GGC CCC ATA CTC CAG GCC

CYP4F20X starfish
         Quoc Wu
         Given its own subfamily renamed CYP4FA1

CYP4F21  sheep
         no accession number
         Johan Bylund
         submitted to nomenclature committee 8/3/99

CYP4F22  human
         GenEMBL AC011492
assembled gene 13 exons 114537-140651 66% to 4F3, 65% to 4F11, 63% to 4F2,
59% to 4F8, 64% to 4F12, 57% to AC011537 exact intron boundaries need checking no ESTs
MLPITDRLLHLLGLEKTAFRIYAVSTLLLFLLFFLFRLLLRFLRLCRSFYITCRRLRCFPQPPRRNWLLGHLGMVS
PNEAGLQDEKKVLDNMHHVLLVWMGPVLPLLVLVHPDYIKPLLGAS
AAIAPKDDLFYGFLKPWLG
DGLLLSKGDKWSRHRRLLTPAFHFDILKPYMKIFNQSADIMH
AKWRHLAEGSAVSLDMFEHISLMTLDSLQKCVFSYNSNCQE
KMSDYISAIIELSALSVRRQYRLHHYLDFIYYRSADGRRFRQACDMVHHFTTEVIQERRR
ALRQQGAEAWLKAKQGKTLDFIDVLLLAR
DEDGKELSDEDIRAEADTFMFEG
HDTTSSGISWMLFNLAKYPEYQEKCREEIQEVMKGRELEELEW
DDLTQLPFTTMCIKESLRQYPPVTLVSRQCTEDIKLPDGRIIPK
GIICLVSIYGTHHNPTVWPDSK
VYNPYRFDPDNPQQRSPLAYVPFSAGPR
NCIGQSFAMAELRVVVALTLLRFRLSVDRTRKVRPELILRTENGLWLKVEPLPPRA*

CYP4F23P human
GenEMBL AC011492 
assembled gene 76% to 4F3, 76% to 4F8, 76% to 4F11, 73% to 4F2, 75% to 4F12, 
77% to 4F11, 60% to other 4F on this accession no ESTs also on AD000091
MSLLSLSWLGLGPVAASPWLLLLLVGASWLLARVLAWTYAFYDNCHRLQCFQQPPKRNCF*GHLSLVS
GNEEDMRLMEDLGHYFRDVQLWWLGSFYPVLHLVHPTFTAPVLQAS AAVALKDMSFYGFLKPWLG
DGLLISAGDKWRWHRHLLTPAFHFKILKPYVKIFNESTNIMH
AKWQRLALEGSVRLEMFEHISLMTLDSLQKCIFSFDSNCQE
KPSEYIDAILELSALSLKRHQHIFLLTDFLYFLTPNGRRFCRACDIVHNFTDAVIQERRR
TLTSQGVDDFLQAKAKSKTLDFIDVLLLAK DENGKKLSDENIRAEADTFMSG
GHDTTASGLSWVLYNLARYPEYQEHCRQEVQELLKNGDPKEIEW
DDLAQLPFLTMCLKESLRLHSPVSRIHRCCPQDGVLPDGRVIPK GNTCTISIFGIHHNPSVWPDPEV
YDPFRFDPENLQKTSPLAFIPFSAVPR
NCIGQTFAMAEMKVVLALTLLRFRVLPDHAEPRRKLELIVRAEDGLWLRVEPLSADLQ* 

CYP4F24P   human
GenEMBL AC011537.4 
77% to 4F11 missing last exon two in frame stops and a gap.
24346 MPQLSLSWLGLGQVAAFPWLLLLLAGASRLLAGFLAWTYAFYDNCRRLQYFPQPPKQKWF 24167
24166 WGQPGX 24152
6633 IIATEEGLKNLTQMSATYPQGFRIWLGPIFPFIVLCHPDIVRSITNAS 6788
8837 AAIAPKDDLSIRFLKPWLGEGILLSGGDK 9016
9017 WSRHRRMLTPAFHFNILKPYIKIFNRSVNIMH 9112
11018 DKWQHLASEGSSRLDMFEHISLMTLDSLQKCIFSFDSHCQE 11140
11921 RPSEYIATILELSALVEKRNQHILQHMDFLYYLSHDGWRFRRACRLVHDFTDAVIQERRH 12100
12101 TLPTQGI 12121
12115 CDFLKNKAKS*TLDFIDVLLLSKXXXXXXXXXXXXXXXXXXXXXX 12189
13460 GHDTTASGLSWVLYNLARHPEYQEHCRQEVQELLKDRDPKEIEWX 13591
21804 DLAQLPFLTMCVKESLRLHPPVPYTSRHRIWDIVLPDGRVIPK 21932
22019 GIICIINIIGIHHNPTV*PDPEV 22165
22263 YNPFRFNSENSKERSPLAFIPFSAGPR 22361

CYP4F25P   HUMAN
           GenEMBL AC018949 
           62% to 4F3 pseudogene
95242 LWRCFLSPTSPCRNPSEYIATILELSALIV*QHQQICLCTDFLYYLTPEGRCFCRACDL 95066
95065 VHNF 95054
95052 DTIILERHCTLTSQGVDDFLNAKATFKIFDFSDAFVLSK 94936

CYP4F26P   Human
           GenEMBL AL139008 
           Homo sapiens chromosome 9 clone RP11-255A11 81% TO 4F25P
SDYVATILELHALIV*WHQQICLCMDFLYYLTPEGRCFCRAYDLVHNF FRAME SHIFT
DTIILEQHCTLTSQSVDILKARATFKTLDFIDALVLSK

CYP4F27P   human
           GenEMBL AC018804.2 
           Homo sapiens clone RP11-397H17 62% TO CYPF25P
21476 LRSCFLSLASPCRNASEHTADILELSTLIV*RRQ*ICLCLDFLYYPFLRGDASAGPVTW 21300
21299 CTTSDTIILQQHRTLTSQGVDDFLKAKATFKASDFIDALVLSK 21171

CYP4F28     Fugu rubripes (pufferfish)
            No accession number
            Scaffold_2425
            76% to 4F7 
8381 MSVLQLALSWTSVCPLLAVLAAAPVALLTFWTAKLLVRHAWYTHRMACFSKPHARSWLLGHLGQ 8190 (0)
7561 MQSTEEGLLQVDELVQMFTYCCSWFIGPFYHLVRVFHPDYVKPLLMAP 7418 (1)
7238 ASITVKDELIYDHLRPWL 7185 (1)
7049 GNSLLLSNGEAWSRRRRLLTPAFHFDILKNYVTKFNTSTNTLH 6921 (0)
6727 DKWHRLLVEGTTNIEVFEHFTLMTLDSLLKCAFSYDSDCQQ 6605 (2)
6527 SSSEYASAIIELSDLIIERRQRILHHWDWIYWRTQQGKRFKKALSIVHK 6381 (2)
6307 FTRDVVQKRLALISQKGVAELAAHRKRDFVDIILLTK 6197 (0)
6115 DEDGKGLTDEELQAEANTFMFA 6050 (1)
5813 GHDTTASAICWSLYNLAHHNHYQEQCRQEVMDLMEGRDVDEIKW 5682 (2)
5607 EDLSSLPFTTMCIRESLRLHSPVQAVTRRYIQDVKLPGERTVPK 5476 (1)
5248 GAICLVSIYGTHHNPAVWTNPH 5183 (0) 
5101 DFDPLRFDPKNQEGLSSHAFIPFSSGPR 5018 (2)
4948 NCIGQKFALAELRVVVALTLLRFRLLPGNDPKRETPFEKVRRLPQLVLRAEGGLWLQIKPVTQTQ* 4751

CYP4F29P    human
            AL109748 chr 21 
            designated CYP4F3LP pseudogene Nature 405, 311-319 2000 
PSEYIATLFELSALIV*WHQQICLCMDFLYYPF 
PEG*CFCRACDLVHNF
DTIILEQPCTLTSQGVHDFLKTKATFKTWDFSDALVLGK 
DENGKELSDEDI*MEAGIFMST
GYDSTATGLY*ILCNLRRHPGVMPSCGARVLRDHNPEDIEW

CYP4F30P    human
            NT_022140.9|Hs2_22296 Homo sapiens chromosome 2 
270169 RRQQICLCWDFLYYPFLRGDASAGSVTWCTTSDTIILQQHRTLTSQGVDDFLKAKATFKA 269990
269989 SDFIDALVLSK 269957

CYP4F31P    human
            NT_019306.9|Hs2_19462 Homo sapiens chromosome 2 
2940797 PEG*CFYKACDLVHNF 2940844

CYP4F32P    human
            NT_033006.1|Hs2_33182 chromosome 2 4F pseudgene 88% to 4F25P
22206 HQQICLCTDFLYYLTPEGRCFCRAYDLVHNF 22114
22112 DTIILEQHLTLTCRGVDDFLKAKATFKILDFSDAFLLSK 21996

CYP4F32P    human
            NT_022300.8|Hs2_22456 chromosome 2 88% to 4F25P
740635 HQQICLCTDFLYYLTPEGRCFCRAYDLVHNF 740727
740729 DTIILEQHLTLTCRGVDDFLKAKATFKILDFSDAFLLSK 740845

CYP4F33P    human
            NT_030040.6|Hs9_30295 chromosome 9 78% TO 4F26P
6944743 HQQICLCTDFLYYLTPEGRCFCGPATWCSTLDTIILERHCTLTSQGVDVLKAKATFKTL 6944567
6944566 DFIDALVPSK 6944537

CYP4F34P    human
            NT_009799.9|Hs13_9956 Homo sapiens chromosome 13 
343178 DTIILEQHCTLTSQGVHDFLKAKATFKT*DFSDALMLGK 343062

CYP4F35P    human
            NT_010859.8|Hs18_11016 chromosome 18 4F pseudogene 84% to 4F12
623264 HQQICLCMDFLYYPFLRGDASAGPMTWCTTLDTIILEQHCTLTSQGVHDFLKAKATFKTW 623085
623084 DFSDALVLGK 623055

CYP4F36P    human
            NT_011281.4|Hs19_11438 chromosome lone last exon 75% TO 4F11
66609 ICMGQVFAVAEMKVVLALTLLGFRFLRHHMEPGRKPELIMHAEGGL*LLVEPLS 66448

Cyp4f37     mouse
            AC073754 comp(28012-47256) 
            93% identical to 4f16  
MLRLSVSGLDLGSVVTLSWHLLLLGAASWILARILAWTYSFCENCSRLRCFPQPPKRNWFLGHLGM
IEHNEEGMQLMTEAGQTYRDVHLCWLGPVIPVLRIVDPAFVAPLLLA TALVAPKDTTFHTFVKPWL
GDGLFLNSGDKWSRHRRLLTPAFHFDILKPYVKIFNQSVNIMH
AKWKHLSSEGSARLEMFEHISLMTLDSLQKCLFGFDSNCQE
SPSEYISAILELSSLVIKRSHQLFLFVDFLYYHTADGRRFRKACDLVHNFTDAVIR
ERRHTLSSQNHDEFLKSKTKSKTLDFIDVLLLAK
DEHGKELSDEDIRAEADTFMFGG
HDTTASALSWILYNLARHPEYQERCRQEVQELLRGREPQEIEW
DDLAQLPFLTMCIKESLRLHPPVIDLLRRCTRDIVLPDGRVIPK
GNICVISIFGIHHNPSVWPDPE
VYDPFRFDPENAHKRPPLAFIPFSAGP
RNCIGQTFAMNEMMVALALTLLRFRILPDDKEPRRKPEIILRAEGGLWLRVEPLS

Cyp4f38p     mouse
             AC073754 comp(9555-20475) 
             partial pseudogene 52% to 4f14
AHILIQVYAAYGNYPHIPKWNWLMGHVSM
PQRRARRMLLNW*GNYP*GFIL*MIPLVSILKPCHPGIAKSV
VSAGDKWSHHCHMLTPAFHFNILKLYVKIFNDSTNIVH
LNISDHVILMTLGNFQKCIFSFDSNCQD
AIMELSMLISRRNKEPLLHMDLLGHLTPDGMCCCKACSMMHGLDDAVI
QERGHILSRQGLDGFFKDKVKFKPLNFIAVLLLTK
DDMSQLPFLTMCISLWLHPPDLLISYYCI*DVVLPDGWIIPKG

Cyp4f39      mouse
             AC073754 range 185296-209241 
             minus strand 62% to 4F14
MLPLTDYLLQLLGLEKTAFRVYV
VSALLLLVLFFFFRLLVRAFKLFSDFRITCRKLSCFPEPPGRHWLLGHMSMVS
PNEKGLQNEKKVLDTMHHIILAWVGPFLPLLVLVHPDYIKPVLGAS
AAIAPKDEFFYSFLKPWLG
DGLLISKGNKWSRHRRLLTPAFHFDILKPYMKIFNQCTNIMH
AKWRRHLAEGSVTSFDMFEHISLMTLDSLQKCVFSYNSDCQE
SMSDYISSIIELSALVVRRQYRLHHYLDFMYYLTADGRRFRQACDTVHNFTTEVI
QERRQALRQQGAEAWLKAKQGKTLDFIDVLLLAK
DEEGKELSDEDIRAEADTFMFEG
HDTTSSGLSWALFNLAKYPEYQEKCREEIQEVMKGRELEELDW
DDLTQLPFTTMCIKESLRQFPPVTLISRRCTEDIKLPDGRVIPK
GIICLVSIYGTHHNPIVWPDSK
VYNPYRFDPDTPQQRSPLAFVPFSAGP
RNCIGQSFAMAEMRVVVALTLLRFRLSVDRTHKVRRKPELILRTENGLWLNVEPLPSRA*

Cyp4f40      mouse
             AC073754 range <1-3688 
             minus strand two N-terminal exons no ESTs
             continues on AC073720 comp(118300-137027) 79% to 4F14
MRYLDLSWLGLGPMSASPWLLFFLVGISWFLARFLTQLYTLYAKCQRLCGFPQPPKKNWFWGHLGMVS
PTEEGMKQVTELVTTYPQGFMTWLGPIVPLITLCHPDIIRSVLSAS
SAAVAPKDDIFYSFLKPWL
GDGLLVSAGDKWNRHRRMLTPAFHFNILKPYVKIFNDSTNIMH
AKWLRLASGGSTRLNMFENISLMTLDTLQKCVFSFNSNCQE
KPSQYIAAILELSTLAVKRNEQLLMHVDLLYRLTPDGMRFYKACRLVHDF
TDAVIQERRRTLLKHGGDDIIKAKAKSKTLDFIDVLLLTK
DEDGKELSDEDIRAEADTFMFRG
HDTTASGLSWILYNLARHPEHQERCRQEVQELLRDRDPKEIEW
DDLAQLPFLTMCIKESLRLHPPVTMVSRCCTQDISLPDGRIIPK
GVICIINIFGTHHNPTVWRDPE
VYDPFRFDPENIQARSPLSFIPFSAGP
RNCIGQTFAMSEMKVALALTLLRFRILPDDKEPRRKPELILRAEGGLWLRVEPLSTQA

Cyp4f41-ps mouse
           Pseudogene (more details later)

CYP4F42    Xenopus laevis
           No accession number
           Yoshiaki Fujita and Hiroaki Ohi
           Submitted to nomenclature committee 7/1/2003
           64% to 4F22 in humans (full length)

CYP4FA1    see bottom of Cyp4 family entries

4G Subfamily

Cyp4g1      Drosophila melanogaster
            no accession number
            Waters,L.C. personal communication (1993)
            Note: called P450-A1 starts at amino acid 192 and goes to 
            the end.  

Cyp4g1      Drosophila melanogaster
            GenEMBL U34328(385bp)
            Dunkov,B.C., Rodriguez-Arniaz,R., Pittendrigh,B.,
            ffrench-Constant,R.H. and Feyereisen,R.
            Cytochrome P450 gene clusters in Drosophila melanogaster.
            Mol. Gen. Genet. 251, 290-297 (1996)

Cyp4g1      Drosophila melanogaster
            GenEMBL AL009188 comp (26402-24747) region genomic

Cyp4g1      Drosophila melanogaster
            GenEMBL AC020093 comp(33262-34932) no introns

CYP4G2      Musca domestica (house fly)
            Dunkov, B.C., R. Rodriguez-Arnaiz, B. Pittendrigh, 
            R. H. ffrench-Constant and R. Feyereisen.
            1996. Cytochrome P450 gene clusters in Drosophila
            melanogaster. Mol. Gen. Genetics 251: 290-297.
            Dta1
            corrected from error, previously listed as 4G6

CYP4G2      Musca domestica (house fly)
            no accession number (559 amino acids)
            Claus Tittiger and Gary Blomquist
            submitted to nomenclature committee

CYP4G3      Musca domestica (house fly)
            Dunkov, B.C., R. Rodriguez-Arnaiz, B. Pittendrigh, 
            R. H. ffrench-Constant and R. Feyereisen. 1996. 
            Cytochrome P450 gene clusters in Drosophila melanogaster. 
            Mol. Gen. Genetics 251: 290-297.
            Dt6-139
            corrected from error, previously listed as 4G5

CYP4G4      Manduca
            Rene Feyereisen
            submitted to nomenclature committee

CYP4G5      Heliothis virescens(tobacco budworm)
            no accession number (382bp)
            Richard ffrench-Constant
            submitted to nomenclature committee
            corrected from error, previously listed as 4G2
            Hv-13a

CYP4G6      Heliothis virescens(tobacco budworm)
            no accession number (386bp)
            Richard ffrench-Constant
            submitted to nomenclature committee
            corrected from error, previously listed as 4G3
            Hv-1

CYP4G7     Tribolium castaneum (red flour beetle)
           no accession number
           Jeff Stuart
           R39
           submitted to nomenclature committee

CYP4G8     Helicoverpa armigera (Australian cotton bollworm) 
           no accesssion number
           Pittendrigh, B., Aronstein, K., Zinkovsky, E., Andreev, O., 
           Campbell, B., Daly, J., Trowell, S. and ffrench-Constant, R.H.
           Cytochrome P450 genes from Helicoverpa armigera: expression in a 
           Pyrethroid susceptible and resistant strain. Submitted
           hacyp24
           submitted to nomenclature committee

CYP4G8v2   Helicoverpa armigera (Australian cotton bollworm) 
           no accesssion number
           Pittendrigh, B., Aronstein, K., Zinkovsky, E., Andreev, O.
           Campbell, B., Daly, J., Trowell, S. and ffrench-Constant, R.H.
           Cytochrome P450 genes from Helicoverpa armigera: expression in a 
           Pyrethroid susceptible and resistant strain. Submitted
           hacyp433
           submitted to nomenclature committee

CYP4G8v3   Helicoverpa armigera (Australian cotton bollworm) 
           no accesssion number
           Pittendrigh, B., Aronstein, K., Zinkovsky, E., Andreev, O., 
           Campbell, B., Daly, J., Trowell, S. and ffrench-Constant, R.H.
           Cytochrome P450 genes from Helicoverpa armigera: expression in a 
           Pyrethroid susceptible and resistant strain. Submitted
           hacyp477g
           submitted to nomenclature committee

CYP4G8v4   Helicoverpa armigera (Australian cotton bollworm) 
           no accesssion number
           Pittendrigh, B., Aronstein, K., Zinkovsky, E., Andreev, O., 
           Campbell, B., Daly, J., Trowell, S. and ffrench-Constant, R.H.
           Cytochrome P450 genes from Helicoverpa armigera: expression in a 
           Pyrethroid susceptible and resistant strain. Submitted
           hacyp73g
           submitted to nomenclature committee

CYP4G9     Helicoverpa armigera (Australian cotton bollworm) 
           no accesssion number
           Pittendrigh, B., Aronstein, K., Zinkovsky, E., Andreev, O., 
           Campbell, B., Daly, J., Trowell, S. and ffrench-Constant, R.H.
           Cytochrome P450 genes from Helicoverpa armigera: expression in a 
           Pyrethroid susceptible and resistant strain. Submitted
           hacyp60
           submitted to nomenclature committee

CYP4G10    Helicoverpa armigera (Australian cotton bollworm) 
           no accesssion number
           Pittendrigh, B., Aronstein, K., Zinkovsky, E., Andreev, O., 
           Campbell, B., Daly, J., Trowell, S. and ffrench-Constant, R.H.
           Cytochrome P450 genes from Helicoverpa armigera: expression in a 
           Pyrethroid susceptible and resistant strain. Submitted
           hacyp5g
           submitted to nomenclature committee

CYP4G11    Apis mellifera (honey bee)
           GenEMBL AF207948
           S. Tares, J.B. BergŽ and M. Amichot.           
           Cloning and expression of cytochrome P450 genes belonging to the 
           CYP4 family and to a novel family, CYP48, in two hymenopteran insects, 
           Trichogramma cacĪciae and Apis mellifera  
           BBRC 268, 677-682 (2000) 
           clone 18Am

CYP4G11v2  Apis mellifera mellifera (honeybee)
           No accession number 
           S. Tares, J.B. BergŽ and M. Amichot.           
           Cloning and expression of cytochrome P450 genes belonging to the 
           CYP4 family and to a novel family, CYP48, in two hymenopteran insects, 
           Trichogramma cacĪciae and Apis mellifera  
           BBRC 268, 677-682 (2000) 
           clone 9Am only 2 amino acid differences, one in EXXR motif, possible PCR 
           errors

CYP4G12    Trichogramma cacĪciae  (a parasitoid wasp)
           GenEMBL AF207949
           S. Tares, J.B. BergŽ and M. Amichot.           
           Cloning and expression of cytochrome P450 genes belonging to the 
           CYP4 family and to a novel family, CYP48, in two hymenopteran insects, 
           Trichogramma cacĪciae and Apis mellifera  
           BBRC 268, 677-682 (2000) 
           clone 3Tc 76% identical to CYP4G8
           clone 6Tc is identical to 3Tc in the coding region but not in a short 
           intron this probably represents a recent gene duplication rather than an 
           allele

CYP4G13     Musca domestica (house fly)
            no accession number
            Xiaohua Shen, Gary Blomquist
            68% identical to 4G1 full length, but different from 4G2 fragment (75%) 
            and 4G3 fragment (71%)
            submitted to nomenclature committee 10/9/98

CYP4G13v2   Musca domestica (house fly)
            no accession number
            Nannan Liu
            98% identical to 4G13, may be same gene with some errors between the 
            two
            submitted to nomenclature committee 7/29/99

CYP4G14     Tribolium castaneum (red flour beetle)
            no accession number
            Jeff Stuart
            S19
            submitted to nomenclature committee

Cyp4g15     Drosophila melanogaster
            no accession number
            M. Ma•bche, D. Bšcking, S. Aragon and C. Dauphin-Villemant.
            submitted to nomenclature committee 6/15/99
            ESTs AI134510, AI388987, AI403684

Cyp4g15     Drosophila melanogaster
            GenEMBL AC013897 comp(8972-10574 and 5306-6643) two pieces

CYP4G16     Anopheles gambiae
            no accession number
            Hilary Ranson
            Submitted to nomenclature committee 8/9/99
            clone name P450A
            79% identical to 4G8

CYP4G16     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPs1x5

CYP4G17     Anopheles gambiae
            no accession number
            Hilary Ranson
            Submitted to nomenclature committee 8/9/99
            clone name P450L
            69% identical to 4G9

CYP4G17     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPs1x3

CYP4G18     Diabrotica virgifera (western corn rootworm) a beetle, Order Coleoptera
            GenEMBL AF243507
            Scharf,M.E., Srinivas,P., Siegfried,B.D. and Meinke,L.J.
            Insecticide Resistance Associated Cytochrome P450 Gene Fragments
            from D. v. virgifera
            Unpublished
            DvP450-2 

CYP4G19     Blattella germanica (German cockroach)
            No accession number 
            Nannan Liu
            Submitted to nomenclature committee 9/24/02
            About 65% identical to Cyp4g15

CYP4G20     Mamestra brassicae (moth)
            GenEMBL deposited but not released yet
            Martine Maibeche 
            73% to 4G16 
            submitted to nomenclature committee Sept. 12, 2003

4H Subfamily

CYP4H1      Anopheles albimanus (mosquito)
            GenEMBL L38682 (382bp) PIR PC2261 (127 amino acids)
            Scott, J.A., Collins,F.H. and Feyereisen, R.
            Diversity of cytochrome P450 genes in the mosquito, Anopheles 
            albimanus.
            Biochem. Biophys. Res. Commun. 205, 1452-1459 (1994)

CYP4H2      Anopheles albimanus (mosquito)
            GenEMBL L38683 (382bp) PIR PC2262 (127 amino acids)
            Scott, J.A., Collins,F.H. and Feyereisen, R.
            Diversity of cytochrome P450 genes in the mosquito, Anopheles 
            albimanus.
            Biochem. Biophys. Res. Commun. 205, 1452-1459 (1994)

CYP4H3      Anopheles albimanus (mosquito)
            GenEMBL L38684 (382bp) PIR PC2263 (127 amino acids)
            Scott, J.A., Collins,F.H. and Feyereisen, R.
            Diversity of cytochrome P450 genes in the mosquito, Anopheles
            albimanus.
            Biochem. Biophys. Res. Commun. 205, 1452-1459 (1994)

CYP4H4      Anopheles albimanus (mosquito)
            GenEMBL L38685 (382bp) PIR PC2264 (127 amino acids)
            Scott, J.A., Collins,F.H. and Feyereisen, R.
            Diversity of cytochrome P450 genes in the mosquito, Anopheles 
            albimanus.
            Biochem. Biophys. Res. Commun. 205, 1452-1459 (1994)

CYP4H5      Anopheles albimanus (mosquito)
            GenEMBL L38686 (382bp) PIR PC2265 (127 amino acids)
            Scott, J.A., Collins,F.H. and Feyereisen, R.
            Diversity of cytochrome P450 genes in the mosquito, Anopheles 
            albimanus.
            Biochem. Biophys. Res. Commun. 205, 1452-1459 (1994)

CYP4H6      Anopheles albimanus (mosquito)
            GenEMBL L38687 (452bp) PIR PC2266 (127 amino acids)
            Scott, J.A., Collins,F.H. and Feyereisen, R.
            Diversity of cytochrome P450 genes in the mosquito, Anopheles 
            albimanus.
            Biochem. Biophys. Res. Commun. 205, 1452-1459 (1994)

CYP4H7      Anopheles albimanus (mosquito)
            GenEMBL L38688 (382bp) PIR PC2267 (127 amino acids)
            Scott, J.A., Collins,F.H. and Feyereisen, R.
            Diversity of cytochrome P450 genes in the mosquito, Anopheles 
            albimanus.
            Biochem. Biophys. Res. Commun. 205, 1452-1459 (1994)

CYP4H8      Anopheles albimanus (mosquito)
            GenEMBL L38689 (461bp) PIR PC2268 (127 amino acids)
            Scott, J.A., Collins,F.H. and Feyereisen, R.
            Diversity of cytochrome P450 genes in the mosquito, Anopheles 
            albimanus.
            Biochem. Biophys. Res. Commun. 205, 1452-1459 (1994)

CYP4H9      Anopheles albimanus (mosquito)
            GenEMBL L38690 (406bp) PIR PC2269 (135 amino acids)
            Scott, J.A., Collins,F.H. and Feyereisen, R.
            Diversity of cytochrome P450 genes in the mosquito, Anopheles 
            albimanus.
            Biochem. Biophys. Res. Commun. 205, 1452-1459 (1994)

CYP4H10    Tribolium castaneum (red flour beetle)
           no accession number
           Jeff Stuart
           R26
           submitted to nomenclature committee

CYP4H11    Tribolium castaneum (red flour beetle)
           no accession number
           Jeff Stuart
           R18
           submitted to nomenclature committee

CYP4H12v1  Culex pipiens pallens (mosquito)
           GenEMBL AF157091
           Zhu Changliang, Dept. of Parasitology, Nanjing University
           submitted to nomenclature committee 5/20/99
           PCR C-terminal fragment 125 amino acids
           clone name NYDR1 73% identical to 4H7 
           this sequence has three frameshifts and some poor quality sequence at    
           the 5 prime end

CYP4H12v2  Culex pipiens
           No accession number
           Changliang Zhu
           Clone NYDR19 PCR fragment 98% identical to 4H12v1
           Submitted to nomenclature committee 9/30/99

CYP4H13    Culex pipiens pallens (mosquito)
           GenEMBL AF160259
           Zhu Changliang, Dept. of Parasitology, Nanjing University
           submitted to nomenclature committee 5/20/99
           PCR C-terminal fragment 151 amino acids
           clone name NYDR3 74% identical to 4H5 
           This sequence has a retained intron

CYP4H14    Anopheles gambiae
           no accession number
           Hilary Ranson
           Submitted to nomenclature committee 8/9/99
           clone name P450P
           94% identical to 4H8

CYP4H14     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPc2r1

CYP4H15    Anopheles gambiae
           no accession number
           Hilary Ranson
           Submitted to nomenclature committee 8/9/99
           clone name P450B
           77% identical to 4H3

CYP4H15     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPc2r2

CYP4H15v2  Anopheles gambiae
           no accession number
           Hilary Ranson
           Submitted to nomenclature committee 8/9/99
           clone name P450Y
           one amino acid difference with 4H15, DNA is 95% identical

CYP4H16    Anopheles gambiae
           no accession number
           Hilary Ranson
           Submitted to nomenclature committee 8/9/99
           clone name P450C
           78% identical to 4H6

CYP4H16     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPn3r1

CYP4H17    Anopheles gambiae
           no accession number
           Hilary Ranson
           Submitted to nomenclature committee 8/9/99
           clone name P450D
           83% identical to 4H16

CYP4H17     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPn3r3

CYP4H18    Anopheles gambiae
           no accession number
           Hilary Ranson
           Submitted to nomenclature committee 8/9/99
           clone name P450G
           77% identical to 4H16

CYP4H18     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.
            Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
            Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
            Science 298, 179-181 (2002)
            Anopheles map name CYPn3r2

CYP4H19    Anopheles gambiae
           no accession number
           Hilary Ranson
           Submitted to nomenclature committee 8/9/99
           clone name P450K
           78% identical to 4H7

CYP4H19     Anopheles gambiae (malaria mosquito)
            GenEMBL 
            Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
            See the paper: Evolution of supergene families associated with 
            insecticide resistance.