Table I

Families of structural domains of P450-containing systems

By Kirill Degtyarenko

Last modified: Mon May 10 11:23:36 BST 2004


P450 enzyme system FAD domain Flavodoxin Fe-S protein Cyt b5 P450

FNR-like

Rabbit microsomal 1
NADPH:P450 reductase
cyt b5* P450
FAD domain FMN domain
S. carbophilus
NADPH:P450 reductase
P450sca
FAD domain? FMN domain?
B. megaterium P450BM-3 (CYP102)
flavin domain
P450 domain
FAD domain FMN domain
F. oxysporum fatty acid hydroxylase
flavin domain
P450 domain
FAD domain? FMN domain?
Mouse macrophage nitric oxide synthase
flavin domain
haem domain
FAD domain FMN domain
Rat microsomal 2 NADH:cyt b5 reductase cyt b5 P450*

ADXR-like [Fe2S2]

Human mitochondrial adx reductase adrenodoxin P450scc (CYP11A), P45011b (CYP11B), P450c27 (CYP27)

GR-like [Fe2S2]

P. putida camphor hydroxylase putidaredoxin reductase putidaredoxin P450cam (CYP101)
Pseudomonas sp. terpineol hydroxylase terpredoxin reductase terpredoxin P450terp (CYP108)
Rhodococcus sp. thiocarbamate hydroxylase rhodocoxin reductase rhodocoxin P450 (CYP116)

GR-like [Fe3S4]

S. griseolus SU1 ? Fd-1 P450 SU1 (CYP105A1)
S. griseolus SU2 ? Fd-2 P450 SU2 (CYP105B1)
S. griseus ? Fd soy P450soy (CYP105D1)
R. fascians ? Fd (FAS2) P450 (FAS1)
S. erythraea Fd reductase erythrodoxin P450eryF (CYP107)

F. oxysporum nitric oxide reductase ? ? ? ? P450nor (CYP55)

* Additional component of redox chain
Abbreviations used: adx, adrenodoxin; ADXR, adrenodoxin reductase; cyt b5, cytochrome b5; Fd, ferredoxin; FNR, ferredoxin:NADP+ reductase; GR, glutathione reductase

Table II

Other enzyme systems sharing domains typical for P450-containing systems


Enzyme system FAD domain Flavodoxin Fe-S protein Cyt b5 Other proteins/domains

FNR-like

Rat microsomal fatty acid desaturase NADH:cyt b5 reductase cyt b5 acyl-CoA desaturase
Human erythrocyte soluble methaemoglobin reductase NADH:cyt b5 reductase cyt b5 globin
Anabaena sp. photosynthetic electron transport chain FNR flavodoxin* [Fe2S2] Fd
N. crassa NADPH:nitrate reductase flavin domain cyt b domain Mo-pterin domain
E. coli sulphite reductase
alpha-subunit (flavoprotein)
ß-subunit (haemoprotein)
FAD domain FMN domain
P. cepacia phthalate dioxygenase
phthalate dioxygenase reductase
oxygenase
flavin domain** [Fe2S2] Fd domain
M. capsulatus soluble methane monooxygenase
component C
component A (alpha-chain, ß-chain, gamma-chain) component B
flavin domain [Fe2S2] Fd domain
Yeast flavohaemoglobin flavin domain globin domain

GR-like

P. putida benzene 1,2-dioxygenase Fd reductase (bnzD) [Fe2S2] Fd (bnzC) oxygenase alpha (bnzA) and ß (bnzB) subunits
P. putida toluene 1,2-dioxygenase Fd reductase (todA) [Fe2S2] Fd (todB) oxygenase alpha (todC1) and ß (todC2) subunits
Pseudomonas sp. biphenyl dioxygenase Fd reductase (bphG) [Fe2S2] Fd (bphF) oxygenase large (bphA) and small (bphE) subunits
P. oleovorans alkane hydroxylase rubredoxin reductase (alkT) rubredoxin (alkG) alkane 1-monooxygenase (alkB)
E. coli thioredoxin system thioredoxin reductase thioredoxin

Yeast flavocytochrome b2 cyt b domain (ß/alpha)8-barrel
H. annuus acyl lipid desaturase cyt b domain acyl-CoA desaturase domain
Chicken sulphite oxidase cyt b domain Mo-pterin domain

* Additional component of redox chain
** Flavin domain of PDR binds FMN
Abbreviations used: cyt b5, cytochrome b5; Fd, ferredoxin; FNR, ferredoxin:NADP+ reductase; GR, glutathione reductase


* To the
graphic representation of P450-containing systems domain structure


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