Selected references on membrane topology of P450s

Last modified: Wed Apr 14 12:57:12 BST 2004

• Black, S.D., Martin, S.T. and Smith, C.A. (1994) Membrane topology of liver microsomal cytochrome P450 2B4 determined via monoclonal antibodies directed to the halt-transfer signal. Biochemistry 33, 6945-6951.
• Brown, C.A. and Black, S.D. (1989) Membrane topology of mammalian cytochromes P-450 from liver endoplasmic reticulum. Determination by trypsinolysis of phenobarbital-treated microsomes. J. Biol. Chem. 264, 4442-4449.
• Chen, C.-D. and Kemper, B. (1997) Different structural requirements at specific proline residue positions in the conserved proline-rich region of cytochrome P450 2C2. J. Biol. Chem. 264, 28607-28611.
• Chen, C.-D., Doray, B. and Kemper, B. (1997) Efficient assembly of functional cytochrome P450 2C2 requires a spacer sequence between the N-terminal signal anchor and catalytic domains. J. Biol. Chem. 272, 22891-22897.
• Dong, M.-S., Bell, L.C., Guo, Z., Phillips, D.R., Blair, I.A. and Guengerich, F.P. (1996) Identification of retained N-formylmethionine in bacterial recombinant mammalian cytochrome P450 proteins with the N-terminal sequence MALLLAVFL...: Roles of residues 3-5 in retention and membrane topology. Biochemistry 35, 10031-10040.
• Edwards, R.J., Murray, B.P., Singleton, A.M. and Boobis, A.R. (1991) Orientation of cytochromes P450 in the endoplasmic reticulum. Biochemistry 30, 71-76.
• Hu, M.-C., Hsu, L.-C., Hsu, N.-C. and Chung, B.-c. (1996) Function and membrane topology of wild-type and mutated cytochrome P-450c21. Biochem. J. 316, 325-329.
• Hsu, L.-C., Hu, M.-C., Cheng, H.-C., Lu, J.-C. and Chung, B.-c. (1993) The N-terminal hydrophobic domain of P450c21 is required for membrane insertion and enzyme stability. J. Biol. Chem. 268, 14682-14686.
• Hudecek, J. and Anzenbacher, P. (1988) Secondary structure prediction of liver microsomal cytochrome P-450; proposed model of spatial arrangement in a membrane. Biochim. Biophys. Acta 955, 361-370.
• Kemper, B. and Szczesna-Skorupa, E. (1989) Cytochrome P-450 membrane signals. Drug Metab. Rev. 20, 811-820.
• Kempf, A.C., Zanger, U.M. and Meyer, U.A. (1995) Truncated human P450 2D6: expression in Escherichia coli, Ni²⁺-chelate affinity purification, and characterization of solubility and aggregation. Arch. Biochem. Biophys. 321, 277-288.
• Kunz, B.C., Rehorek, M., Hauser, H., Winterhalter, K.H. and Richter, C. (1985) Decreased lipid order induced by microsomal cytochrome P-450 and NADPH-cytochrome P-450 reductase in model membranes: fluorescence and electron spin resonance studies. Biochemistry 24, 2889-2895.
• Menzel, R., Kärgel, E., Vogel, F., Böttcher, C. and Schunck, W.-H. (1996) Topogenesis of a microsomal cytochrome P450 and induction of endoplasmic reticulum membrane proliferation in Saccharomyces cerevisiae. Arch. Biochem. Biophys. 330, 97-109.
• Miller, J.P., Herbette, L.G. and White, R.E. (1996) X-ray diffraction analysis of cytochrome P450 2B4 reconstituted into liposomes. Biochemistry 35, 1466-1474.
• Nelson, D.R. and Strobel, H.W. (1988) On the membrane topology of vertebrate cytochrome P-450 proteins. J. Biol. Chem. 263, 6038-6050.
• Nelson, D.R. and Strobel, H.W. (1989) Secondary structure prediction of 52 membrane-bound cytochromes P450 shows a strong structural similarity to P450cam. Biochemistry 28, 656-660.
• Neve, E.P.A., Eliasson, E., Pronzato, M.A., Albano, E., Marinari, U. and Ingelman-Sundberg, M. (1996) Enzyme-specific transport of rat liver cytochrome P450 to the Golgi apparatus. Arch. Biochem. Biophys. 333, 459-465.
• Pernecky, S.J. and Coon, M.J. (1996) N-terminal modifications that alter P450 membrane targeting and function. Methods Enzymol. 272, 25-34.
• Pernecky, S.J., Larson, J.R., Philpot, R.M. and Coon, M.J. (1993) Expression of truncated forms of liver microsomal P450 cytochromes 2B4 and 2E1 in Escherichia coli: influence of NH₂-terminal region on localization in cytosol and membranes. Proc. Natl. Acad. Sci. USA 90, 2651-2655.
• Pernecky, S.J., Olken, N.M., Bestervelt, L.L. and Coon, M.J. (1995) Subcellular localization, aggregation state, and catalytic activity of microsomal P450 cytochromes modified in the NH₂-terminal region and expressed in Escherichia coli. Arch. Biochem. Biophys. 318, 446-456.
• Sakaki, T., Akiyoshi-Shibata, M., Yabusaki, Y. and Ohkawa, H. (1992) Organella-targeted expression of rat liver cytochrome P450c27 in yeast. Genetically engineered alteration of mitochondrial P450 into a microsomal form creates a novel functional electron transport chain. J. Biol. Chem. 267, 16497-16502.
• Scheller, U., Kraft, R., Schroder, K.L. and Schunck, W.-H. (1994) Generation of the soluble and functional cytosolic domain of microsomal cytochrome P450 52A3. J. Biol. Chem. 269, 12779-12783.
• Scheller, U., Juretzek, T. and Schunck, W.-H. (1996) Generation of the cytosolic domain of microsomal P450 52A3 after high-level expression in Saccharomyces cerevisiae. Methods Enzymol. 272, 65-75.
• Schwarz, D., Chernogolov, A. and Kisselev, P. (1999) Complex formation in vesicle-reconstituted mitochondrial cytochrome P450 systems (CYP11A1 and CYP11B1) as evidenced by rotational diffusion experiments using EPR and ST-EPR. Biochemistry 38, 9456-9464.
• Shank-Retzlaff, M.L., Raner, G.M., Coon, M.J. and Sligar, S.G. (1998) Membrane topology of cytochrome P450 2B4 in Langmuir-Blodgett monolayers. Arch. Biochem. Biophys . 359, 82-88.
• Shumyantseva, V.V., Kuznetsova, G.P., Uvarov, V.Yu. and Archakov, A.I. (1994) Membrane topology of N-terminal residues of cytochromes P-450 2B4 and 1A2. Biochem. Mol. Biol. Int. 34, 183-190.
• Sueyoshi, T., Park, L., Moore, R., Juvonen, R.O. and Negishi, M. (1995) Molecular engineering of microsomal P450 2a-4 to a stable, water-soluble enzyme. Arch. Biochem. Biophys. 322, 265-271.
• Szczesna-Skorupa, E. and Kemper, B. (1989) NH₂-terminal substitutions of basic amino acids induce translocation across the microsomal membrane and glycosylation of rabbit cytochrome P450IIC2. J. Cell Biol. 108, 1237-1243.
• Szczesna-Skorupa, E. and Kemper, B. (1991) Cell-free analysis of targeting of cytochrome P450 to microsomal membranes. Methods Enzymol. 206, 64-75.
• Szczesna-Skorupa, E. and Kemper, B. (1993) An N-terminal glycosylation signal on cytochrome P450 is restricted to the endoplasmic reticulum in a luminal orientation. J. Biol. Chem. 268, 1757-1762.
• Szczesna-Skorupa, E. and Kemper, B. (2000) Endoplasmic reticulum retention determinants in the transmembrane and linker domains of cytochrome P450 2C1. J. Biol. Chem. 275, 19409-19415.
• Szczesna-Skorupa, E., Browne, N., Mead, D. and Kemper, B. (1988) Positive charges at the NH₂ terminus convert the membrane-anchor signal peptide of cytochrome P-450 to a secretory signal peptide. Proc. Natl. Acad. Sci. USA 85, 738-742.
• Szczesna-Skorupa, E., Straub, P. and Kemper, B. (1993) Deletion of a conserved tetrapeptide, PPGP, in P450 2C2 results in loss of enzymatic activity without a change in its cellular location. Arch. Biochem. Biophys. 304, 170-175.
• Szczesna-Skorupa, E., Ahn, K., Chen, C.-D., Doray, B. and Kemper, B. (1995) The cytoplasmic and N-terminal transmembrane domains of cytochrome P450 contain independent signals for retention in the endoplasmic reticulum. J. Biol. Chem. 270, 24327-24333.
• Szczesna-Skorupa, E., Chen, C.-D., Rogers, S. and Kemper, B. (1998) Mobility of cytochrome P450 in the endoplasmic reticulum membrane. Proc. Natl. Acad. Sci. USA 95, 14793-14798.
• Tretiakov, V.E., Degtyarenko, K.N., Uvarov, V.Yu. and Archakov, A.I. (1989) Secondary structure and membrane topology of cytochrome P450s. Arch. Biochem. Biophys. 275, 429-439.
• Uvarov, V.Yu., Sotnichenko, A.I., Vodovozova, E.L., Molotkovsky, J.G., Kolesanova, E.F., Lyulkin, Yu.A., Stier, A., Krüger, V. and Archakov, A.I. (1994) Determination of membrane-bound fragments of cytochrome P-450 2B4. Eur. J. Biochem. 222, 483-489.
• Uvarov, V.Yu., Kolesanova, E.F., Apletalina, E.V., Kozin, S.A. and Archakov, A.I. (1995) Surface of cytochrome P-450 2B4: structure and function. Vestnik Ross. Akad. Med. Nauk (Moscow) 2, 29-35.
• Uvarov, V.Yu., Ivanov, Yu.D., Romanov, A.N., Gallyamov, M.O., Kiselyova, O.I. and Yaminski, I.V. (1996) Scanning tunneling microscopy study of cytochrome P450 2B4 incorporated in proteoliposomes. Biochimie 78, 780-784.

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