Age-dependent changes in liver microsomal cytochrome P-450.

Compiled by Andrey L. Sukhodub. All comments are welcomed.

last modified:

CYP P-450	Species	Character of changes observed	Method of estimation used	Ref
IA1	rat	Absent in fetal liver till 14 day of pregnancy	IMA *	[1]
		From 15th day of pregnancy constitutive gene expression and further developmental mRNA accumulation occurs	mRNA	[2]
		Detected at low levels in rats of both sexes at ages 1 week-24 months.	IMA, 6b-testosterone-hydroxylase	[3]
		Remain unchanged in 12-36 months period	IMA	[4]
	hamster	Peak expression was observed in 6-month old hamsters and declined in older ones	IMA	[44]
	mouse	In B10.RIII mice progressively decrease between 4 and 28 months; in C57BL/10 mice little or no changes with age	mRNA	[5, 6]
	rabbit	Absent in embryos, in newborn - low level; sharply accumulates till 1-2 months after birth; maximal level in 2-6 months, then decline	mRNA	[7, 8]
	man	In adult liver microsomes was not detected. Located in lungs and placenta.	IMA	[9, 35]
		Immunoreactive antibodies detected in fetal liver.	IMA	[39]
IA2	rat	Constitutive level versus total content of P-450 is higher in 1-day then in 15-day rats, but the amount of immunoreactive form is not depends upon age	IMA	[10]
		Detected at low levels in rats of both sexes and ages (1 week-24 months)	IMA, 6b-testosterone-hydroxylase	[3]
		Remains unchanged in the age 12-36 months	IMA, mRNA	[4, 11]
	hamster	Peak expression was observed in 6-month old hamsters and declined in older ones	IMA	[44]
	mouse	Decrease between age 4-5 months and 30-31 months	mRNA	[6]
	rabbit	Present at low level or absent in the new born animal up to week 3, sharply accumulated between weeks 3 and 4 to reach a maximum by week 2. After 2nd month of life begin to fall.	mRNA, IMA	[7, 8, 40]
	man	Absent in the microsomes from fetal and neonatal livers. Increased in infants aged 1-3 months to attain 50% of the adult value at one year.	IMA, imipramine demethylation, mRNA	[9, 12]
		Unaltered with advancing age. Up to 60-fold variability between livers.	IMA	[13, 35]
		Activity falls in elderly	caffeine clearance	[34]
IB1	man	The level of expression was very low in adult liver and only three out of six fetal livers expressed CYP1B1.	mRNA	[43]
IIA1	rat	The level was maximal at 3 weeks of age in rats of both sexes	IMA, testosterone 7a-hydroxylation	[3, 33]
		In males decrease in postsuckling period	testosterone 7a-hydroxylation	[14, 15]
		The level and activity elevated in the old rats. Female predominant	IMA, mRNA, testosterone 7a-hydroxylation	[16]
IIA2	rat	Detected in male rats only and was induced developmentally. Disappeared at 24 months of age.	IMA, testosterone 15a-hydroxylation	[3]
		Had no changes with aging	IMA	[4]
IIA6	rat	3-week-old rats had the highest activity	coumarin 7-hydroxylation	[34]
	man	No differences between perinatal and infant and after infancy patients	IMA	[17]
		About 4% of total content. Minor form. Large, (up to 170-fold) variability.	IMA, specific probes	[18, 35]
IIB1/B2	rat	4 fold increase in 24 hours after birth	mRNA	[19]
		Decrease with aging	mRNA	[20]
		Detected at low levels in rats of both sexes at 1 week to 24 months. Had no changes with aging	IMA, testosterone 16b-hydroxylation	[3, 4]
	hamster	Content reached the maximum at the age of 6 months and maintained almost the same level thereafter (18 month-old)	IMA	[44]
IIB4	rabbit	Developmentally increase in postnatal period till 2 months. In 2-6 months period the level is constant, then decrease	IMA	[7]
IIB6	man	Expressed highly in subjects over 1 year old.	IMA	[17]
		The minor P-450 form (less then 1% of total content)	IMA, specific substrates	[18]
IIC3	rabbit	Increased in postnatal period between 2-3 weeks, then between 1-2 months	IMA	[7]
IIC6	rat	In males appears in a 4 weeks after birth, then permanently increase between 4-12 weeks. In females mRNA discovered in a small amounts on the 2 week, the level increase till 4 weeks old, after 7 weeks begin to fall.	mRNA	[22]
		The amount is equal in male and female liver.	IMA	[3]
	hamster	Peak expression was observed in 6-month old hamsters and declined in older ones	IMA	[44]
IIC7	rat	Increases gradually over the first 6 weeks of life.	mRNA	[22]
		The content increase from less then 1% in the young (3 weeks old) males and females to 7-14% in adult (6 weeks). Female predominant.	IMA	[23]
IIC8	man	Absent in fetal liver. Appears in a month after birth. Bimodal distribution in the liver.	IMA, mRNA	[12, 24, 35]
IIC9	man	Absent in fetal liver. Appears in a month after birth.	IMA, mRNA	[12, 24]
		Had no changes with age	IMA	[17]
IIC11	rat	Detected in male rats only and induced developmentally. Disappears at 24 months of age.	IMA, testosterone 2a-hydroxylation	[3]
		Dramatic increase at puberty, between 4,5-5,5 weeks of life.	mRNA	[22, 25]
	hamster	Content reached the maximum at the age 6 month and maintained almost the same level thereafter (18 month-old)	IMA	[44]
IIC12	rat	Female-specific form. Induced developmentally in female rats and was also detected in male rats at 3 weeks and 24 months of age.	testosterone 15a-hydroxylation	[3, 25]
IIC13	rat	The content increase from 1% in 3 weeks animals to 17% in 6 weeks old of total P-450 content. Male predominant.	IMA	[23]
IIC18	man	Absent in fetal liver and appears in 1 months after birth. Polymorphic.	IMA, mRNA	[24, 35]
IIC23	rat	Maximal expression in early neonates and remained quite stable.	mRNA	[26]
IID6	man	Appears soon after birth.	IMA	[9]
		Had no changes with age.	IMA	[17]
		About 2% of the total P-450 content. Polymorphic.	IMA, specific substrates	[18, 35]
IIE1	rat	Had a little volume or absent in fetal liver, increase in a 4 days after birth and remains high during the first 17 days of neonate.	N-nitrosodimethylamine demethylation	[27]
		In postsuckling period decrease more in males then in females. Female predominant.		[14]
	hamster	Peak expression was observed in 6-month old hamsters and declined in older ones	IMA	[44]
	rabbit	Not detectable until 2 weeks and increase rapidly to approximately twice the adult level at 5 weeks of age.	mRNA	[28]
		Adult age isoform.		[7]
	man	Protein and its associated activity could not be detected in fetal liver and rise during the first few hours following birth independently of the gestational age (between 25-40 weeks). During this period mRNA level remains fairly low. From 1 months to 1 year the protein content gradually increases and is accompanied by the accumulation of mRNA.	IMA, mRNA	[29]
		Present in liver samples from perinatal, infant and 1 year old patients and no differences was observed between these groups.	IMA	[17]
		In fetal liver present a protein immunoreactive with CCYP2E1 antibodies that exhibited a slightly lower molecular weight than that found in adult liver samples..	IMA	[41]
		Negative association between age and protein content.	IMA	[13]
		About 7% from total P-450 content in adult liver. Up to 50-fold variability.	IMA	[18, 35]
IIE2	rabbit	Specific young age isoform	IMA	[7]
		mRNA detectable immediately after birth and reaches slightly greater than the adult level at 2 weeks of age.	mRNA	[28]
IIIA1	rat	The main constitutive male form. 10 fold increase during the first 24 hours after birth.	mRNA	[19, 42]
IIIA2	rat	Constitutively expressed early after birth and characterized by an extinction in the adult females after 3 weeks. In males disappeared when the rats were 24 months of age. In female was detected only at 1 and 3 weeks of age.	IMA, testosterone 6b-hydroxylation, mRNA	[3, 36, 42]
	hamster	Peak expression was observed in 6-month old hamsters and declined in older ones	IMA	[44]
IIIA3/4	man	Absent in fetal liver	IMA	[30]
		Extremely weak in the fetus and began to raise after birth to reach 30-40% of the adult activity after 1 month. mRNA accumulation displays a similar pattern of evolution. Major P-450 isoform present in the adult liver. Up to 60-fold variability. Constitutive, about 50% of total content.	testosterone 6b-hydroxylation, mRNA, IMA	[31, 9, 35]
IIIA5	man	Detected in statistically higher percentage of children and adolescents (19 years old and under. Detected in 1 of 10 man fetal livers. Polymorphic - present in 27% of population.	IMA	[32]
IIIA6	rabbit	Present at low level in the new born animal up to week 3, sharply accumulated between weeks 3 and 4 to reach maximum by week 4 and decreased in adult.	mRNA	[7, 8]
IIIA7	mice	The mRNA epression could be detected as early as the 15th embryonic day and increased gradually with advancing gestation, but then so after birth. The protein epression was also detectable postnatally and increased.	MRNA, IMA	[38]
	man	Expressed in the fetal liver. Activity high in the fetal liver and maximal during the first week following birth before to progressively decline and reached a very low level in adult livers.	16a-hydroxylation of dehydroepiandrosterone, IMA	[31, 17]
		Found in all tested samples of fetal liver as in 7 of 13 adult livers. Expressed at similar levels in fetal liver from either gender.	mRNA	[37]
IIIA9	rat	Detected only in adult rats, which a nearly twofold higher expression in females.	mRNA	[36]
IIIA18	rat	Observed before and after puberty at fairly constant	mRNA	[36]
IIIA23		levels that were about 20% higher in males than in females
IVA1	hamster	Level did not change from 3 to 6 months, and then declined to about 40% of the younger level at 12 and 18 months of age.	IMA	[44]
IVA2	rat	Detected in male rats only and was induced developmentally. Disappeared in male at 24 months of age.	IMA	[3]
IVA3	rat	Induced developmentally and detected at similar level in rats of both sexes at age 1 week-2 months.	IMA	[3]

* IMA - immunoanalyse

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