Accession numbers for NADPH:P450 reductases

Compiled by Kirill N. Degtyarenko (kirill@icgeb.trieste.it)

last modified:

NADPH:P450 reductases
Haemoprotein/NADPH:P450 reductase fusion proteins
NADPH:sulphite reductases flavoprotein alpha-component

Species abbreviations

The eukaryotic microsomal P450 system contains two components: NADPH:P450 reductase (CPR), a flavoprotein containing both FAD and FMN, and P450. CPR appears to be a fusion protein consisting of domains which are homologous to flavodoxin (FMN domain) and ferredoxin:NADP+ reductase (FAD domain). A soluble monooxygenase P450BM-3 from Bacillus megaterium exists as a single polypeptide chain with two functional parts, the haem (P450) and flavin (CPR) domains, and represents a unique bacterial one-component P450 system. Mammalian nitric oxide synthase (NOS) contains FAD, FMN and haem and was the first eukaryotic self-sufficient P450-like system described. The C-terminal domain of NOS is clearly homologous to CPR, whereas its N-terminal (oxygenase) domain has a unique fold different from that of P450s. alpha-Component of bacterial NADPH:sulphite reductase contains FAD and FMN and is similar to CPR.


A. NADPH:P450 reductases (EC 1.6.2.4)

                                                                           
 Gene   Species      PIR     SwissProt       GenBank/EMBL/DDBJ            PRF   
                                                                                
CPR       rat  A36073          P00388  M10068  M12516  J00718  S70373  1701250A
                                                                       1103351A
                                                                       1203241A
          rab  A25505          P00389  D00101  X04610                  1211284A
          mou                  P37040  D17571                          2017207A
          ham                  Q60451  D83230
          hum  A60557          P16435  T08991  T15414                  1515351A
                                                                       1516146A
                                                                       1808183A
                               Q16455  S90469
          gpi  S27158          P37039  D10498                          1904143A
          pig  A25584          P04175  L33893                          1302145A
                                                                       1107181A
                                                                       1207184A
                                                                       1103184A
          tro  A28577          P19618                                  1309284A
          Dme                  Q27597  X93090
          Dmt                  P91655  U65749
          Mdo                  Q07994  L19897  S62221  S62726  S62730  2005235A
          Cel                  Q09590  U21322
                                       Z35595
          Sce  A41447          P16603  D00316  D13788                  1408205A
          Ctr  A37890          P37201  M35199                          1616317A
          Cma  S49140          P50126  X76226  D25327                         
          Spo  S29123          P36587  X64702  S46250                  1819195A
                               Q09744  Z54140
          Ani  S38427          Q00141  Z26938                          2119198A
          Ath  S21530                  X66016                         
          Ath  S21531                  X66017                         
          Cro  S31502          Q05001  X69791  Y09417
          Htu  S37157          Q96561  Z26250
          Htu  S37156          Q96560  Z26251
          Htu                  Q42830  U58629
          Pau  A47298          P37116  L07843                          1911469A
          Psm                  Q40916  Z49767
          Vsa  S37159          Q43235  Z26252
          pea                  O04434  AF002698

MIPS protein superfamily: 0121.0

MIPS protein domain family: 00062


B. Haemoprotein/NADPH:P450 reductase fusion proteins

                                                                           
 Gene   Species      PIR     SwissProt       GenBank/EMBL/DDBJ            PRF   
                                                                                

P450/NADPH:P450 reductase (EC 1.14.14.1/1.6.2.4)


CYP102    Bme  A34286          P14779  J04832                          1510269A
CYP102    Bsu                  O08336  U93874
Cyp2b-10  mou                          X99715
 + CPR                                

Nitric Oxide Synthases (EC 1.14.13.39)


eNOS      bov  A42841  A46033  P29473  M99057  L27056  M95674  M89952  1815263A
                                                                       1817310A
                                                                       1905357A
          hum  S24052  A42867  P29474  L10693  L10694  L10695  L10696  1816206A
                                       L10697  L10698  L10699  L10700  1817302A
                                       L10701  L10702  L10703  L10704  1920328A
                                       L10705  L10706  L10707  L10708 
                                       L10709                         
                                       X76303  X76304  X76305  X76306 
                                       X76307  X76308  X76309  X76310 
                                       X76311  X76312  X76313  X76314 
                                       X76315  X76316                 
                                       M95296  L23210  M93718  L26914 
                                       D26607 
          gpi                          U76736
          mou                          U53142
          pig                          U59924
          rat                  Q62600  U02534
          she                          U76738                  

nNOS      hum  S28878          P29475  L02881  D16408  U31466
                                       U17299  U17300  U17301  U17302
                                       U17303  U17304  U17305  U17306
                                       U17307  U17308  U17309  U17310
                                       U17311  U17312  U17313  U17314
                                       U17315  U17316  U17317  U17318
                                       U17319  U17320  U17321  U17322
                                       U17323  U17324  U17325  U17326
                                       U66360  U66361  U66362
          gpi                          U76737
          mou  JN0609                  D14552                         
          rat  S16233          P29476  X59949  U67309                  1712320A
          she                          X99042
          Dme                          U25117
          Rhp                          U59389

iNOS      hum  A47475  S47566  P35228  U05810  X73029  L09210  L26055  2001203A
                                       D29675  L24553  T15817  D26525 
                                       U31511  X81701  X81702  Z49251
                                       X85759  X85760  X85761  X85762
                                       X85763  X85764  X85765  X85766
                                       X85767  X85768  X85769  X85770 
                                       X85771  X85772  X85773  X85774 
                                       X85775  X85776  X85777  X85778 
                                       X85779  X85780  X85781
          mou  A43271  A46186  P29477  M87039  L09126  L23806  M84373  1807286A
               JN0458                  M92649  U43428                  1808352A
          rat  JC1472  JN0457  Q06518  D14051  X76881  L12562  U26686  1909128A
               S40126  S47647          D12520  U03699  L36063  S71597
               S38253                  X95629  Z69839  D44591  U48829
                                       D83661
          pig                          U59390
          bov                  Q27995  U18331
          got                          U29085
          chi                  Q90703  U34045  U46504  D85422
          gol                          X97603
          tro                          X97013

iNOSP     hum                          U65689  U65690  U65691  U65692
                                       U65693  U65694  U65695  U65696
                                       U65697  U65698  U65699

C. NADPH:sulphite reductases flavoprotein alpha-component (EC 1.8.1.2)


 Gene   Species      PIR     SwissProt       GenBank/EMBL/DDBJ            PRF  

cysJ      Sty  A34231          P38039  M23007  J05009                  1515279A
cysJ      Eco  B34231          P38038  M23008  J05057
cysJ      Trp  S34190                  Z23169

met10     Sce  S45174  JC2514  P39692  L26503  D44610  U05820          2020353A
met10     Scb                          L26504                          2020353B

MIPS protein superfamily: 3633.0

MIPS protein domain family: 00062

Pfam families: PF00175, PF00258

To the flavodoxins page ( ).

To the ferredoxin:NADP+ reductase superfamily page ( ).

To the Todd Porter's CPR page


Species abbreviations

Ani = Aspergillus niger
Ath = Arabidopsis thaliana
Bme = Bacillus megaterium
bov = bovine
Cal = Candida albicans
Cel = Caenorhabditis elegans (nematode worm)
chi = chicken
Cma = Candida maltosa
Ctr = Candida tropicalis
Dme = Drosophila melanogaster (fruit fly)
Dmt = Drosophila mettleri
Eco = Escherichia coli
Fox = Fusarium oxysporum
gol = goldfish (Carassius auratus)
got = goat
gpi = guinea pig
ham = hamster
Htu = Helianthus tuberosus (Jerusalem artichoke)
hum = human
Mdo = Musca domestica (house fly)
mou = mouse
Pau = Phaseolus aureus (syn. Vigna radiata, mung bean)
pig = pig
Psm = Pseudotsuga menziesii
rab = rabbit
rat = rat
Rhp = Rhodnius prolixus
Scb = Saccharomyces carlsbergensis
Sce = Saccharomyces cerevisiae (baker's yeast)
she = sheep
Spo = Schizosaccharomyces pombe
Sty = Salmonella typhimurium
tro = trout
Trp = Thiocapsa roseopersicina
Vsa = Vicia sativa


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