Accession numbers for reductase component of three component-type P450-containing systems

Compiled by Kirill N. Degtyarenko (

last modified:

NADPH:adrenodoxin reductases
(P450-specific) ferredoxin:NADH reductases
NADH:cytochrome b5 reductases

Species abbreviations

Mitochondrial (1) and most bacterial P450-containing systems (2) have three components: an FAD-containing flavoprotein (NADPH or NADH-dependent reductase), an iron-sulphur protein, and P450.

   NADPH -->  adrenodoxin reductase  -->  adrenodoxin  --> P450    (1)
    NADH -->  ferredoxin  reductase  -->  ferredoxin   --> P450    (2)
It was shown that in microsomal P450-containing systems cytochrome b5 can serve as electron donor for P450s:
    NADH --> cytochrome b5 reductase --> cytochrome b5 --> P450    (3) 
Despite functional similarities, reductase components of redox chains (1, 2, 3) are not homologous. Bacterial P450-specific ferredoxin reductases belong to FAD-dependent pyridine nucleotide reductase family, whereas cytochrome b5 reductase belongs to flavoprotein pyridine nucleotide cytochrome reductase family. Adrenodoxin reductase shows no sequence similarity with other known ferredoxin reductases over the entire length of sequence.

NAD(P)H:Fe-S reductases:

 Gene   Species      PIR     SwissProt       GenBank/EMBL/DDBJ            PRF   

A. NADPH:adrenodoxin reductase (EC

adxR      bov  A29604  JT0751  P08165  M17029  D00211  X13736          1309175A
                                       D83472  D83473  D83474  D83475  1314208A
          hum  A36482  A40487  P22570  M58508  M58509  J03826          1413353A
               B40487                                                  1701475A
          mou  I49671                  D49920
          Sce                  P48360  U28038  U28373  U38689

MTCY31.14 Mtu                  Q10547  Z73101

B. (P450-specific) ferredoxin:NADH reductases (EC 1.18.1.-)

camA      Psp  JX0078          P16640  J05406  D00528  M12546          1603298A

terpA     Pse  S27654  D42971  P33009  M91440                          

forA      Ser                          L38646                          2110282B

thcD      Rho                  P43494  U17130                          2104333K

To the FAD-dependent pyridine nucleotide oxidoreductases page ( ).

C. NADH:cytochrome b5 reductases (EC

 Gene   Species      PIR     SwissProt       GenBank/EMBL/DDBJ            PRF   
DIA1      bov  A23896          P07514  M83104                          1111250A
          hum  JS0468  B26616  P00387  M16461  M16462  T09077  M28705  1203280A
                                       M28706  M28707  M28708  M28709  1516206A
                                       M28710  M28711  M28712  M28713  1707155A
                                       S79503  S79504
          rat  JX0119          P20070  J03867  X77117  D00718  D00636  1413360A
               S23641  S42567          X65190  X65191                  1612299A
          ART                          U24681
cbr1      Sce  S49935          P38626  Z28365                          2006246A
mcr1      Sce  A55196          P36060  Z28150  Z26877                  2102246A

MIPS protein superfamily: 0120.0

MIPS protein domain family: 00050

Pfam family: PF00175

To the ferredoxin:NADP+ reductase superfamily page ( ).

Species abbreviations

ART = artificial gene
bov = bovine
hum = human
Mtu = Mycobacterium tuberculosis
Pse = Pseudomonas species
Psp = Pseudomonas putida
rat = rat
Rho = Rhodococcus species
Sce = Saccharomyces cerevisiae (baker's yeast)
Ser = Saccharopolyspora erythraea

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