Research Groups

Sneh Lata Singla-Pareek

Plant Stress Biology

Group Leader

Research Interests and Description

Sneh Lata Singla-Pareek

International Centre for Genetic Engineering and Biotechnology
Aruna Asaf Ali Marg
110 067 New Delhi, India

E-mail: snehicgeb.res.in
Office Tel: +91-11-26742357
Office Fax: +91-11-26742316

Education

Department of Botany, University of Delhi, India, BSc (Hons), 1989
Plant Molecular Biology, University of Delhi, India, MSc, 1991
Plant Molecular Biology, University of Delhi, India, PhD, 1997

Career History

Since 2001, Staff Research Scientist, Plant Molecular Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
2005, Visiting Scientist, Cornell University, Ithaca, NY, USA.
2000-2001, Research Scientist, Plant Molecular Biology, ICGEB, New Delhi.
1999, Research Scientist, Department of Genetics, University of Delhi, South Campus, New Delhi.
1998, Postdoc Fellow, Dept. of Biology, University of North Carolina, NC, USA.
1997, Scientist, Centre for Plant Molecular Biology, University of Delhi, South Campus, New Delhi.

Awards
2006, Innovative Young Biotechnologist, DBT, Ministry of Science and Technology, Government of India.
2005, Visiting Scientist Fellowship Award, Cornell University, USA.
1995, “Travel Fellowship by UNESCO” Award.
1994, Indian Scientist Award, International Congress of Biochemistry and Molecular Biology, New Delhi, India.
1989, South Campus Scholarship.

Teaching Activity

ICGEB Ph.D. Course, ICGEB New Delhi.

Scientific Activity

Soil salinity is a global problem and a solution to this problem by any method, e.g. conventional breeding, molecular markers or transgenic approach, will create an opportunity for cultivation of crops in soils affected by salinity. In an effort to contribute towards the goal of raising plants with better suitability with respect to deteriorating edaphic factors, we have isolated several novel stress responsive genes, which have been characterized employing the contemporary tools and techniques. Out of these set of genes, a few have been functionally validated employing the tools of functional genomics. Among these, those involved in removing the cellular toxicity, as mediated via glyoxalase pathway warrants special mention. This is so because the transgenic plants overexpressing both Gly I and Gly II enzymes, have been found to be able to tolerate multiple stresses such as salinity, drought as well as heavy metals. The biochemical basis of this improved tolerance has also been addressed to an extent. At this moment, we have joined hands with a commercial company for development of product(s) that can withstand and successfully grow in saline soils. Successful development of salinity tolerant rice plants will not only bring uncultivable land under active cultivation, but also pave way for use of this technology in other crops for similar use.

Selected publications

Ghosh A, Kushwaha HR, Hasan MR, Pareek A, Sopory SK, Singla-Pareek SL. 2015. Presence of unique glyoxalase III proteins in plants indicates the existence of shorter route for methylglyoxal detoxification. Sci Reports (In Press)

Nongpiur RC, Singla-Pareek SL, Pareek A. 2015. Genomics Approaches for Improving Salinity Stress Tolerance in Crop Plants.  Current Genomics (In Press)

Joshi R, Karan R, Singla-Pareek SL, Pareek A. 2015. Ectopic expression of Pokkali phosphoglycerate kinase-2 (OsPGK2-P) improves yield in tobacco plants under salinity stress. Plant Cell Rep. 35: 27-41

Kaur C, Kushwaha HR, Mustafiz A, Pareek A, Sopory SK, Singla-Pareek SL. 2015. Analysis of global gene expression profile of rice in response to methylglyoxal indicates its possible role as a stress signal molecule. Front Plant Sci. 6:682.

Singh AK, Kumar R, Tripathi AK, Gupta BK, Pareek A, Singla-Pareek SL. 2015. Genome-wide investigation and expression analysis of Sodium/Calcium exchanger gene family in rice and Arabidopsis. Rice (N Y). 8(1):54.

Singh A, Kushwaha HR, Soni P, Gupta H, Singla-Pareek SL, Pareek A. 2015. Tissue specific and abiotic stress regulated transcription of histidine kinases in plants is also influenced by diurnal rhythm. Front Plant Sci. 6:711

Das P, Nutan KK, Singla-Pareek SL, Pareek A. 2015.  Understanding salinity responses and adopting 'omics-based' approaches to generate salinity tolerant cultivars of rice. Front Plant Sci. 6:712.

Tripathi, A.K., Singh, K., Pareek, A., Singla-Pareek, S.L. 2015. Histone chaperones in Arabidopsis and rice: genome-wide identification, phylogeny, architecture and transcriptional regulation. BMC Plant Biol 15:42.

Kaur, C., Kumar, G., Pareek, A., Singla-Pareek, S.L. 2015. Molecular cloning and characterization of Salt Overly Sensitive gene promoter from Brassica juncea (BjSOS2). Mol Biol Rep 42(6): 1139-1148.

Sharma, R., Mishra, M., Gupta, B., Parsania, C., Singla-Pareek, S.L., Pareek, A. 2015. De Novo assembly and characterization of stress transcriptome in a salinity tolerant variety CS52 of Brassica juncea. PLoS One 10.1371/journal.pone.0126783.

Ghosh A, Pareek A and Singla-Pareek SL (2015). Stress Tolerance Assay at the Seed Germination Stage for Tobacco. Bio-protocol 5(7): e1441.

Ghosh A, Pareek A and Singla-Pareek SL (2015). Leaf Disc Stress Tolerance Assay for Tobacco. Bio-protocol 5(7): e1440.

Das, P., Nutan, K.K., Singla-Pareek, S.L., Pareek, A. 2015. Oxidative environment and redox homeostasis in plants: dissecting out significant contribution of major cellular organelles. Front Environ Sci 2, 70.                      

Lakra, N., Nutan, K., Das, P., Anwar, K., Singla-Pareek, S.L., Pareek, A. 2015. A nuclear-localized histone-gene binding protein from rice (OsHBP1b) functions in salinity and drought stress tolerance by maintaining chlorophyll content and improving the antioxidant machinery. J Plant Physiol 176:36-46.

Kumari, S., Joshi, R., Singh, K., Roy, S., Tripathi, A.K., Singla-Pareek, S.L., Pareek, A. 2015. Expression of a cyclophilin OsCyp2-P isolated from a salt-tolerant landrace of rice in tobacco alleviates stress via ion homeostasis and limiting ROS accumulation. Funct Integr Genomics 15: 395-412.

Ghosh, A., Pareek, A., Sopory, S.K., Singla-Pareek, S.L. 2014. A glutathione responsive rice glyoxalase II, OsGLYII-2, functions in salinity adaptation by maintaining better photosynthesis efficiency and anti-oxidant pool. Plant J 80(1):93-105.

Mustafiz, A., Ghosh, A., Tripathi, A.K., Kaur, C., Ganguly, A.K., Bhavesh, N.S., Tripathi, J.K., Pareek, A., Sopory, S.K., Singla-Pareek, S.L. 2014. A unique Ni2+-dependent and methylglyoxal-inducible rice glyoxalase I possesses a single active site and functions in abiotic stress response. Plant J 78(6), 951-963.

Kaur, C., Singla-Pareek, S.L., Sopory, S.K. 2014. Stress response of OsETHE1 is altered in response to light and dark conditions. Plant Signal Behav 9(11):e973820.

Kaur, C., Singla-Pareek, S.L., Sopory, S.K. 2014. Glyoxalase and methylglyoxal as biomarkers for plant stress tolerance. Crit Rev Plant Sci 33(6): 429-456.

Kaur, C., Ghosh, A., Pareek, A., Sopory, S.K., Singla-Pareek, S.L. 2014. Glyoxalases and Stress Tolerance in Plants. Biochem Soc Trans 42(2), 485-490.

Kaur, C., Mustafiz, A., Sarkar, A., Ariyadasa, T., Singla-Pareek, S.L., Sopory, S.K. 2014. Expression of multiple stress inducible ETHE1-like protein from rice is higher in roots and is regulated by calcium. Physiol Plant 152(1), 1-16.

Kushwaha, H.R., Singla-Pareek, S.L., Pareek, A. 2014. Putative osmosensor - OsHK3b – a histidine kinase protein from rice show high structural conservation with its ortholog AtHK1 from Arabidopsis. J Biomol Struct Dyn 32(8), 1318-1332.

Kaur, C., Vishnoi, A., Ariyadasa, T.U., Bhattacharya, A., Singla-Pareek, S.L., Sopory, S.K. 2013. Episodes of horizontal gene-transfer and gene-fusion led to co-existence of different metal-ion specific glyoxalase I. Sci Rep 3, 3076.

Soda, N., Kushwaha, H.R., Soni, P., Singla-Pareek, S.L., Pareek, A. 2013. A suite of new genes defining salinity stress tolerance in seedlings of contrasting rice genotypes. Funct Integr Genomics 13(3), 351-365.

Soni, P., Kumar, G., Soda, N., Singla-Pareek, S.L., Pareek, A. 2013. Salt overly sensitive pathway members are influenced by diurnal rhythm in rice. Plant Signal Behav 8(7).

Mukhopadhyay, P., Singla-Pareek, S.L., Reddy, M.K., Sopory, S.K. 2013. Stress-mediated alterations in chromatin architecture correlate with down-regulation of a gene encoding 60S rpL32 in rice. Plant Cell Physiol 54(4), 528-540.

Sahoo, K.K., Tripathi, A.K., Pareek, A., Singla-Pareek, S.L. 2013. Taming Drought stress in rice through genetic engineering and transcription factors and protein kinases. Plant Stress Sp. Issue 1: 60-72.

Lakra, N., Nutan, K.K., Singla-Pareek, S.L., Pareek, A. 2013. Modulating the expression of transcription factors: an attractive strategy for raising abiotic stress tolerant plants. Plant Stress Sp. Issue 1: 84-99.

Mukhopadhyay, P., Singla-Pareek, S.L., Reddy, M.K., Sopory, S.K. 2013. Regulation of stress responsive genes in plants: Involvement of epigenetic mechanisms. Plant Stress Sp. Issue 1: 35-46.

Kumari, S., Roy, S., Singh, P., Singla-Pareek, S.L., Pareek, A. 2013. Cyclophilins: Proteins in search of function. Plant Signal Behav 8(1).

Tripathi, A.K., Pareek, A., Sopory, S.K., Singla-Pareek, S.L. 2012. Narrowing down the targets for yield improvement in rice under normal and abiotic stress conditions via expression profiling of yield-related genes. Rice 5(1), 37.

Kumar, G., Kushwaha, H.R., Panjabi-Sabharwal, V., Kumari, S., Joshi, R., Karan, R., Mittal, S., Singla-Pareek, S.L., Pareek, A. 2012. Clustered metallothionein genes are co-regulated in rice and ectopic expression of OsMT1e-P confers multiple abiotic stress tolerance in tobacco via ROS scavenging. BMC Plant Biol 12, 107.

Nongpiur, R., Soni, P., Karan, R., Singla-Pareek, S.L., Pareek, A. 2012. Histidine kinases in plants: Cross talk between hormone and stress responses. Plant Signal Behav 7(10), 1230-1237.

Kumar, R., Mustafiz, A., Sahoo, K.K., Sharma, V., Samanta, S., Sopory, S.K., Pareek, A.  Singla-Pareek, S.L. 2012. Functional screening of cDNA library from a salt tolerant rice genotype Pokkali identifies mannose-1-phosphate guanyl transferase gene (OsMPG1) as a key member of salinity stress response. Plant Mol Biol 79(6), 555-568.

Singh, A.K., Kumar, R., Pareek, A., Sopory, S.K. Singla-Pareek, S.L. 2012. Overexpression of Rice CBS Domain Containing Protein Improves Salinity, Oxidative, and Heavy Metal Tolerance in Transgenic Tobacco. Mol Biotechnol 52(3), 205-216.

Sharma, S., Mustafiz, A., Singla-Pareek, S.L., Srivastava, P.S., Sopory, S.K. 2012. Characterization of stress and methylglyoxal inducible triose phosphate isomerase (OscTPI) from rice. Plant Signal Behav 7(10), 1337-1345.

Kumar, G., Kushwaha, H.R., Singla-Pareek, S.L., Pareek, A. 2012. Cloning, structural and expression analysis of OsSOS2 in contrasting cultivars of rice under salinity stress. Genes Genomes Genomics 6, 34-41.

Tripathy, M.K., Tyagi, W., Goswami, M., Kaul, T., Singla-Pareek, S.L., Deswal, R., reddy, M.K., Sopory, S.K. 2012. Characterization and Functional Validation of Tobacco PLC Delta for Abiotic Stress Tolerance. Plant Mol Biol Rep 30(2), 488-497.

Sahoo, K.K., Tripathi, A.K., Pareek, A., Sopory, S.K., Singla-Pareek, S.L. 2011. An improved protocol for efficient transformation and regeneration of diverse indica rice cultivars. Plant Methods 7(1), 49.

Singh, K., Singla-Pareek, S.L., Pareek, A. 2011. Dissecting out the crosstalk between salinity and hormones in roots of Arabidopsis. OMICS 15(12), 913-924.

Kushwaha, H.R., Kumar, G., Verma, P.K., Singla-Pareek, S.L., Pareek, A. 2011. Analysis of a salinity induced BjSOS3 protein from Brassica indicate it to be structurally and functionally related to its ortholog from Arabidopsis. Plant Physiol Biochem 49(9), 996-1004.

Samanta, S., Reddy, M.K., Singla-Pareek, S.L., Sopory, S.K. 2011. Stress and development specific regulation of alternative splicing in plants. J Plant Biol 37(1), 19-30.

Mukhopadhyay, P., Reddy, M.K., Singla-Pareek, S.L., Sopory, S.K. 2011. Transcriptional Downregulation of Rice rpL32 Gene under Abiotic Stress Is Associated with Removal of Transcription Factors within the Promoter Region. PLoS One 6(11), e28058.

Saxena, M., Roy, S.D., Singla-Pareek, S.L., Sopory, S.K., Bhalla-Sarin, N. 2011. Overexpression of the Glyoxalase II gene leads to enhanced salinity tolerance in Brassica juncea. Open Plant Sci J 5, 23-28.

Soda, N., Lakra, N., Singla-Pareek, S.L., Pareek, A. 2011. Abiotic stress-related studies in plants: Journey towards proteomics era. J Plant Physiol 37, 31-46

Mustafiz, A., Singh, A.K., Pareek, A., Sopory, S.K., Singla-Pareek, S.L. 2011. Genome-wide identification of glyoxalase genes and their expression profiling during development and in response to abiotic stresses in Arabidopsis and rice. Funct Integr Genomics 11(2), 293-305.

Mustafiz, A., Sahoo, K.K., Singla-Pareek, S.L., Sopory, S.K. 2010. Metabolic engineering of glyoxalase pathway for enhancing stress tolerance in plants. Methods Mol Biol 639, 95-118.

Touhidul Islam, S.M., Tammi, R.S., Singla-Pareek, S.L., Seraj, Z.I. 2010. Enhanced salinity tolerance and improved yield properties in Bangladeshi rice Binnatoa through Agrobacterium-mediated transformation of PgNHX1 from Pennisetum glaucum. Acta Physiol Plant 32, 657-663.

El-Shabrawi, H., Kumar, B., Kaul, T., Reddy, M.K., Singla-Pareek, S.L., Sopory, S.K. 2010. Redox homeostasis, antioxidant defense, and methylglyoxal detoxification as markers for salt tolerance in Pokkali rice. Protoplasma 245, 85-96.

Kushwaha, H.R., Singh, A.K., Sopory, S.K., Singla-Pareek, S.L., Pareek, A. 2009. Genome wide expression analysis of CBS domain containing proteins in Arabidopsis thaliana (L.) Heynh and Oryza sativa L. reveals their developmental and stress regulation. BMC Genomics 10, 200.

Singla-Pareek, S.L., Yadav, S.K., Mustafiz, A., Sopory, S.K. 2009. Role of the glyoxalase pathway in delaying plant senescence under stress conditions. SEB Exp Biol Series 62,171-185.

Karan, R., Singla-Pareek, S.L., Pareek, A. 2009. Histidine kinase and response regulator genes as they relate to salinity tolerance in rice. Funct Integr Genomics 9(3), 411-417.

Kumari, S., Singh, P., Singla-Pareek, S.L., Pareek, A. 2009. Heterologous expression of a salinity and developmentally regulated rice cyclophilin gene (OsCyp2) in E. coli and S. cerevisiae confers tolerance towards multiple abiotic stresses. Mol Biotechnol 42(2), 195-204.

Kumar, G., Purty, R.S., Singla-Pareek, S.L., Pareek, A. 2009. Maintenance of stress related transcripts in tolerant cultivar at a level higher then sensitive one appears to be a conserved salinity response among plants. Plant Signal Behav 4(5), 431-434.

Kumar, G., Purty, R.S., Sharma, M.P., Singla-Pareek, S.L., Pareek, A. 2009. Physiological responses among Brassica species under salinity stress show strong correlation with transcript abundance for SOS pathway related genes. J Plant Physiol 166(5), 507-520.

Kumari, S., Panjabi nee Sabharwal, V., Kushwaha, H.R., Sopory, S.K., Singla-Pareek, S.L., Pareek, A. 2009. Transcriptome map for seedling stage specific salinity stress response indicates a specific set of genes as candidate for saline tolerance in Oryza sativa L. Funct Integr Genomics  9(1), 109-123.

Tohidul Islam, S.M., Tammi, R.S., Singla-Pareek, S.L., Seraj, Z.I. 2009. Agrobacterium-mediated transformation and constitutive expression of PgNHX1 from Pennisetum glaucum L. in Oryza sativa L. cv. Binnatoa. Plant Tissue Cult Biotech 19, 25-33.

Singla-Pareek, S.L., Yadav, S.K., Mustafiz, A., Sopory, S.K. 2008. Detoxification of methylglyoxal by genetic manipulation of glyoxalase pathway leads to salt stress tolerance in crop plants. Suppl Plant Cell Physiol Vol 49 pp S0026-S0026

Singla-Pareek, S.L., Yadav, S.K., Pareek, A., Reddy, M.K., Sopory, S.K. 2008. Enhancing  salt tolerance in a crop plant by overexpression of glyoxalase II. Transgenic Res 17(2), 171-180.

Yadav, S.K., Singla-Pareek, S.L., Sopory, S.K. 2008. An overview on the role of methylglyoxal and glyoxalases in plants. Drug Metabol Drug Interac 23 (1-2), 51-68.

Singh, A.K., Ansari, M.W., Pareek, A., Singla-Pareek, S.L. 2008. Raising Salinity Tolerant Rice: Recent Progress and Future Perspectives. Physiol Mol Biol Plants 14(1-2), 137-154.

Purty, R.S., Kumar, G., Singla-Pareek, S.L., Pareek, A. 2008. Towards Salinity Tolerance in Brassica: an Overview. Physiol Mol Biol Plants 14(1-2), 39-49.

Verma, D., Singla-Pareek, S.L., Rajagopal, D., Reddy, M.K., Sopory, S.K. 2007. Functional validation of a novel isoform of Na+/H+ antiporter from Pennisetum glaucum for enhancing salinity tolerance in rice. J Biosci 32 (3), 621-628.

Rajgopal, D., Agarwal, P., Tyagi, W., Singla-Pareek, S.L., Reddy, M.K., Sopory, S.K. 2007. Characterization of an isoform of Na/H antiporter from Pennisetum glaucum that confers high level of salinity tolerance in transgenic Brassica juncea. Mol Breeding 19, 137-151.

Yadav, S.K., Singla-Pareek, S.L., Kumar, M., Pareek, A., Saxena, M., Sarin, N.B., Sopory, S.K. 2007. Characterization and functional validation of glyoxalase II from rice. Protein Expr Purif 51(1), 126-132.

Singla-Pareek, S.L., Yadav, S.K., Pareek, A., Reddy, M.K., Sopory, S.K. 2006. Transgenic tobacco overexpressing glyoxalase pathway enzymes grow and set viable seeds in zinc spiked soils. Plant Physiol 140(2), 613-623.

Pareek, A., Singh, A., Kumar, M., Kushwaha, H.R., Lynn, A.M., Singla-Pareek, S.L. 2006. Whole genome analysis of Oryza sativa L. reveals similar architecture of two-component-signaling machinery with Arabidopsis. Plant Physiol 142(2), 380-397.

Tyagi, W., Singla-Pareek, S.L., Nair, S., Reddy, M.K., Sopory, S.K. 2006. A novel isoform of ATPase c subunit from pearl millet that is differentially regulated in response to salinity and calcium. Plant Cell Rep 25(2), 156-163.

Yadav, S.K., Singla-Pareek, S.L., Reddy, M.K., Sopory, S.K. 2005. Transgenic tobacco plants overexpressing glyoxalase enzymes resist an increase in methylglyoxal and maintain higher reduced glutathione levels under salinity stress. FEBS Letters 579(27), 6265-6271.

Yadav, S.K., Singla-Pareek, S.L., Ray, M., Reddy, M.K., Sopory, S.K. 2005. Methylglyoxal levels in plants under salinity stress are dependent on glyoxalase I and glutathione. Biochem Biophys Res Commun 337(1), 61-67.

Tyagi, W., Rajagopal, D., Singla-Pareek, S.L., Reddy, M.K., Sopory, S.K. 2005. Cloning and regulation of a stress regulated Pennisetum glaucum Vacuolar ATPase c gene and characterization of its promoter that expresses in shoot hairs and floral organs. Plant Cell Physiol 46(8), 1411-1422.

Yadav, S.K., Singla-Pareek, S.L., Reddy, M.K., Sopory, S.K. 2005. Methylglyoxal detoxification by glyoxalase system: A survival strategy during environmental stresses. Physiol Mol Biol Plants 11(1), 1-11.

Singla-Pareek, S.L., Reddy, M.K., Sopory, S.K. 2003. Genetic engineering of glyoxalase pathway in tobacco leads to enhanced salinity tolerance. Proc Natl Acad Sci U S A 100(25), 14672-14677.

Belanger, K.D., Wyman, A.J., Sudol, M.N., Singla-Pareek, S.L., Quatrano Ralph, S. 2003. A signal peptide secretion screen in Fucus distichus embryos reveals expression of glucanase, EGF domain-containing,and LRR receptor kinase-like polypeptides during asymmetric cell growth. Planta 217(6), 931-950.

Kumar, B., Singla-Pareek, S.L., Reddy, M.K., Sopory, S.K. 2003. Glutathione: Biosynthesis, Homeostasis and its role in abiotic stresses. J Plant Biol 30, 179-188.

Mishra, R.N, Singla-Pareek, S.L, Nair, S., Sopory, S.K, Reddy, M.K. 2002. Directional genome walking using PCR. Biotechniques 33, 830-832, 834

ICGEB New Delhi

ICGEB Campus
Aruna Asaf Ali Marg
110 067 New Delhi
INDIA
Tel: +91-11-26741358/1007
Fax: +91-11-26742316
icgebicgeb.res.in

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