Research Groups

Shams

Microbial Engineering

Group Leader

Research Interests and Description

Syed Shams Yazdani

International Centre for Genetic Engineering and Biotechnology
Aruna Asaf Ali Marg
110 067 New Delhi, India

E-mail: shamsicgeb.res.in

Link to DBT-ICGEB Bioenergy Centre

Education

Jawaharlal Nehru University, New Delhi, India, PhD (Biotechnology), 2000
Jawaharlal Nehru University, New Delhi, India, MSc (Biotechnology), 1994
Aligarh Muslim University, Aligarh, India, BSc (Hons) Chemistry, 1992

Career History

Since 2012, Coordinator of the DBT-ICGEB Centre for Advanced Bioenery Research, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India 
Since 2011, Group Leader, Synthetic Biology and Biofuel Group, ICGEB, New Delhi
2007-2008, Postdoctoral Associate, Department of Chemical and Biomolecular Engineering, Rice University, Houston, USA
2003-2010, Staff Research Scientist, Malaria Group, ICGEB, New Delhi
1999-2003, Research Scientist, Malaria Group, ICGEB, New Delhi

Teaching Activity

Tutoring activities in the ICGEB PhD program.

Scientific Activity

Cellulosic biomass, such as those present in agricultural and forestry residues, has become a desirable non-food feedstock for the production of fuels and chemicals due to its abundant availability and low price.  The cellulosic biomass is first hydrolyzed to release monomeric sugars, then fermented to ethanol or other products. The high cost of saccharifying enzymes and the inability of traditional yeast, Saccharomyces cerevisiae, to ferment pentose sugars present in biomass in significant quantity into ethanol are the major roadblocks in the commercialization of lignocellulosic ethanol.

Insects living on the agricultural and woody residues harbor variety microbial flora in their guts to help them digest their food. We have studied various kinds of insects living in diverse ecological niches and developed assay procedure to identify and characterize gut celluloytic microbes. Since mixture of enzymes will be needed to hydrolyze the cellulosic biomass, we made bi-functional chimera to introduce the function of two enzymes into single polypeptide and shown that the activity of both the functional domains of the chimera remain intact. We also studied the rational for some of the fungi being hyper active in degrading plant biomass.

Lignocellulosic biomass may consists of 30% of pentose sugars, and therefore its effective fermentation will certainly have a positive impact on the economy of biofuel production. E. coli is a robust host for various genetic manipulations and has been used commonly for bioconversion of hexose and pentose sugars into valuable products. One of the products that E. coli makes under fermentative condition is ethanol. However, availability of limited reducing equivalence and generation of competing co-products undermine ethanol yield and productivity. In our lab, we have constructed an E. coli strain to produce ethanol from hexose and pentose sugars by modulating the expression of pyruvate dehydrogenase and acetate kinase and by deleting pathways for competing co-products. The engineered E. coli strain constructed via endogenous pathway engineering fermented glucose and xylose to ethanol with high yield and productivity. This strain lacking any foreign gene for ethanol fermentation is likely to be genetically more stable and is a better candidate for testing further for the fermentation of lignocellulosic hydrolysate at higher scale. This engineered E. coli strain also showed capabilities to ferment cellobiose present in the hydrolysate into ethanol when a gene responsible for secretion of β-glucosidase was cloned in it. The engineering effort was further extended for production of short chain alcohols and alkane.


See related news item on research concerning Biofuels

Selected publications

Shakeel, T., Fatma, Z., Fatma, T., Yazdani, S.S. 2015. Heterogeneity of alkane chain length in freshwater and marine cyanobacteria. Frontiers in Bioengineering and Biotechnology 16, 3-34 Link to article

Munjal, N., Jawed, K., Wajid, S., Yazdani, S.S. 2015. A Constitutive Expression System for Cellulase Secretion in Escherichia coli and Its Use in Bioethanol Production. PLoS One 10(3):e0119917 PubMed link

Adlakha, N., Yazdani, S.S. 2015. Efficient production of (R,R)-2,3-butanediol fromcellulosic hydrolysate using Paenibacillus polymyxa ICGEB2008. JIMB. 42, 21-8 Link

Singh, R., Mattam, A.J., Jutur, P.P., Yazdani, S.S. 2015. Synthetic Biology in Biofuels Production. Advances in Molecular Cell Biology and Molecular Medicine: Synthetic Biology. edited by R.A. Meyers. Vol. II Wiley-VCH Verlag GmbH & Co., pp. 665-698 Link to text

Adlakha, N., Pfau, T., Ebenhöh, O., Yazdani, S.S. 2015. Insight into metabolic pathways of the potential biofuel producer, Paenibacillus polymyxa ICGEB2008. Biotechnol Biofuels. 8:159. doi: 10.1186/s13068-015-0338-4. eCollection 2015.
Ogunmolu, F.E., Kaur, I., Gupta, M., Bashir, Z., Pasari, N., Yazdani, S,S. 2015. Proteomics Insights Into The Biomass Hydrolysis Potentials of a Hypercellulolytic Fungus Penicillium funiculosum. J. Proteome Res. DOI: 10.1021/acs.jproteome.5b00542 Link to article

Ogunmolu, F.E., Kaur, I., Gupta, M., Bashir, Z., Pasari, N., Yazdani, S,S. 2015. Proteomics Insights Into The Biomass Hydrolysis Potentials of a Hypercellulolytic Fungus Penicillium funiculosum. J. Proteome Res. DOI: 10.1021/acs.jproteome.5b00542 Link to article

Chitnis, C.E., Mukherjee, P., Mehta, S., Yazdani, S.S., Dhawan, S., Shakri, A.R., Bharadwaj, R., Gupta, P.K., Hans, D., Mazumdar, S., Singh, B., Kumar, S., Pandey, G., Parulekar, V., Imbault, N., Shivyogi, P., Godbole, G., Mohan, K., Leroy, O., Singh, K., Chauhan, V.S. 2015. Phase I Clinical Trial of a Recombinant Blood Stage Vaccine Candidate for Plasmodium falciparum Malaria Based on MSP1 and EBA175. PLoS One. 10(4):e0117820 Link to article

Shakeel, T., Fatma, Z., Fatma, T., Yazdani, S.S. 2015. Heterogeneity of alkane chain length in freshwater and marine cyanobacteria. Frontiers in Bioengineering and Biotechnology 16, 3-34 Link to article

Munjal, N., Jawed, K., Wajid, S., Yazdani, S.S. 2015. A Constitutive Expression System for Cellulase Secretion in Escherichia coli and Its Use in Bioethanol Production. PLoS One 10(3):e0119917 PubMed link

Adlakha, N., Yazdani, S.S. 2015. Efficient production of (R,R)-2,3-butanediol fromcellulosic hydrolysate using Paenibacillus polymyxa ICGEB2008. JIMB. 42, 21-8 Link

Singh, R., Mattam, A.J., Jutur, P.P., Yazdani, S.S. 2015. Synthetic Biology in Biofuels Production. Advances in Molecular Cell Biology and Molecular Medicine: Synthetic Biology. edited by R.A. Meyers. Vol. II Wiley-VCH Verlag GmbH & Co., pp. 665-698 Link to text

Mattam, A.J., Yazdani, S.S. 2013. Engineering E. coli strain for conversion of short chain fatty acids to bioalcohols. Biotechnol Biofuels 10, 128 (PubMed link)

Bashir, Z., Kondapalli, V.K., Adlakha, N., Sharma, A., Bhatnagar, R.K., Chandel, G., Yazdani, S.S. 2013. Diversity and functional significance of cellulolytic microbes living in termite, pill-bug and stem-borer guts. Sci Rep 3, 2558 (PubMed link)

Mattam, A.J., Clomburg, J.M., Gonzalez, R., Yazdani, S.S. 2013. Fermentation of glycerol and production of valuable chemical and biofuel molecules. Biotechnol Lett. 35, 831-842 (PubMed link)

Garg, S., Agarwal, S., Kumar, S., Yazdani, S.S., Chitnis, C.E., Singh, S. 2013. Calcium-dependent permeabilization of erythrocytes by a perforin-like protein during egress of malaria parasites. Nat Commun 4, 1736 (PubMed link)

Adlakha, N., Ritturaj Kushwaha, H., Rajagopal, R., Yazdani, S.S. 2013. Draft Genome Sequence of the Paenibacillus sp. Strain ICGEB2008 (MTCC 5639) Isolated from the Gut of Helicoverpa armigera. Genome Announc 1 (PubMed link)

Gupta, S., Adlakha, N., Yazdani, S.S. 2013. Efficient extracellular secretion of an endoglucanase and a β-glucosidase in E. coli. Protein Expr Purif 88, 20-25 (PubMed link)

Ahmad, I., Fatma, Z., Yazdani, S.S., Kumar, S. 2013. DNA barcode and lipid analysis of new marine algae potential for biofuel. Algal Research 2: 10-15

Munjal, N., Mattam, A.J., Pramanik, D., Srivastava, P.S., Yazdani, S.S. 2012. Modulation of endogenous pathways enhances bioethanol yield and productivity in Escherichia coli. Microb Cell Fact.11(1):145. [Epub ahead of print]

Adlakha, N., Sawant, S., Anil, A., Lali, A., Yazdani, S.S. 2012. Specific fusion of β-1,4- endoglucanase and β-1,4- glucosidase enhances the cellulolytic activity and helps in channeling of the intermediates. Appl Environ Microbiol 78, 7447-7454 PubMed link

Adlakha, N., Rajagopal, R., Kumar, S., Reddy, V.S., Yazdani, S.S. 2011. Synthesis and characterization of chimeric proteins based on cellulase and xylanase from an insect gut bacterium. Appl Environ Microbiol 77, 4859-4866 PubMed link

Yazdani, S.S., Mattam, A.J., Gonzalez, R. 2010. Fuel and chemical production from glycerol, a biodiesel waste product. In: “Biofuels from Agricultural Wastes and Byproducts”. Blaschek H., Ezeji T., and Scheffran, J. (Eds.). Blackwell Publishing, Ames, IA, USA

Chauhan, V.S., Yazdani, S.S., Gaur, D. 2010. Malaria vaccine development based on merozoite surface proteins of Plasmodium falciparum. Hum Vaccin 6 [Epub ahead of print] PubMed PMID: 20861668 PubMed link

Mazumdar, S., Sachdeva, S., Chauhan, V.S., Yazdani, S.S. 2010. Identification of cultivation condition to produce correctly folded form of a malaria vaccine based on Plasmodium falciparum merozoite surface protein-1 in Escherichia coli. Bioprocess Biosyst Eng. 33, 719-730 PubMed link

Yazdani, S.S., Mattam, A.J., Gonzalez, R. 2010. Fuel and chemical production from glycerol, a biodiesel waste product. In: “Biofuels from Agricultural Wastes and Byproducts”. Blaschek H., Ezeji T., Scheffran, J. Eds. Blackwell Publishing, Ames, IA, USA

Gonzalez, R., Murarka, A., Dharmadi, Y., Yazdani, S.S. 2008. A new model for the anaerobic fermentation of glycerol in enteric bacteria: trunk and auxiliary pathways in Escherichia coli. Metabolic Engineering 10, 234-245 PubMed link

Yazdani, S.S., Gonzalez, R. 2008. Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products. Metab Eng 10, 340-351 PubMed link

Yazdani, S.S., Gonzalez, R. 2007. Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. Curr Opin Biotechnol 18, 213-219 PubMed link

Yazdani, S.S., Mukherjee, P., Chauhan, V.S., Chitnis, C.E. 2006. Immune responses to asexual blood-stages of malaria parasites. Curr. Mol. Med. 6, 187-203

Yazdani, S.S., Shakri, A.R., Pattnaik, P., Rizvi, M.M., Chitnis, C.E. 2006. Improvement in yield and purity of a recombinant malaria vaccine candidate based on the receptor-binding domain of Plasmodium vivax Duffy binding protein by codon optimization. Biotechnol. Lett. 28, 1109-1114

Yazdani, S.S., Shakri, A.R., Chitnis, C.E. 2004. A high cell density fermentation strategy to produce recombinant malarial antigen in E. coli. Biotechnol. Lett. 26, 1891-1895

Yazdani, S.S., Shakri, A.R., Mukherjee, P., Baniwal, S.K., Chitnis, C.E. 2004.  Evaluation of immune responses elicited in mice against a recombinant malaria vaccine based on Plasmodium vivax Duffy binding protein. Vaccine 22, 3727-3737

Patents

Yazdani, S.S., Mattam, A.J. 2013. Engineering E. coli strain for conversion of short chain fatty aids to bioalcohols. Indian Patent Application No. 2651/DEL/2013 Link

Yazdani, S.S., Munjal, N., Mattam, A.J. 2013. Modified bacteria for the production of bioalcohols. International Application No. WO2014033759A1 Link

Yazdani SS, Adlakha N. 2012. Novel cellulolytic enzymes and their chimera for hydrolysis of lignocellulosic biomass. Indian Patent Application No. 2695/DEL/2012 Link

Yazdani, S.S., Munjal, N., Mattam, A.J. 2012 Modified bacteria for the production of bioalcohols. Indian Patent Application No. 2696/DEL/2012 Link

ICGEB New Delhi

ICGEB Campus
Aruna Asaf Ali Marg
110 067 New Delhi
INDIA
Tel: +91-11-26741358/1007
Fax: +91-11-26742316
icgebicgeb.res.in

tl_files/iTunes_Badge_Color_master_10251024.png

 

 

tl_files/FB.png tl_files/Twitter_logo_blue-1def.png tl_files/rss.png
tl_files/LinkedIn-InBug-2CRev2.png tl_files/YouTube.png