Research Groups

Plant RNAi Biology

Group Leader

Research Interests and Description

Neeti Sanan-Mishra

International Centre for Genetic Engineering and Biotechnology
Aruna Asaf Ali Marg
110 067 New Delhi, India

E-mail: neetiicgeb.res.in
Tel: +91-11-26741358, ext. 280
Fax: +91-11-26742316

Education

Jawaharlal Nehru University, New Delhi, India, PhD, 1997
Jawaharlal Nehru University, New Delhi, India, MSc, 1992
University of Delhi, India, BSc, 1990

Career History

Since 2006, Staff Research Scientist, Plant Molecular Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
2000-2005, Research Associate, ICGEB, New Delhi, India
1998-1999, Research Scientist, University of Delhi, South Campus, New Delhi, India

Teaching Activity

ICGEB PhD Course on "Molecular Biology of Plant Stress"

Scientific Activity

The group is involved in understanding the RNAi mechanisms operative in rice plants in response to increasing soil salinity, high temperature and virus infection. The endeavour is to use this knowledge for crop improvement and protection through employing RNAi based technology. This assumes importance in the era of global climate change, as new approaches are required to address the emerging challenges for sustainable agriculture. 

microRNAs (miRNAs) have been recognized as important regulatory factors in plant development and disease expression. We have adopted a two-pronged strategy of using bioinformatic predictions along with the traditional molecular biology and biochemical approaches to understand the miRNA regulatory networks operative in rice during abiotic (salt and temperature) and biotic (viruses) stresses. We have deep sequenced and analyzed the small RNAs in response to salt stress, viral infection and developmental cues from different local rice varieties. Work has been initiated to analyze the spatial and temporal accumulation of miRNAs to understand the fine- tuning of target gene expression in plant development. Few novel sequences have been identified and validated. Already 14 sequences have been deposited in mirBase (Osa-miR 2918-2932). This information is being used to tap the natural variation existent in the Indian rice cultivars and generate a small RNA database for rice in the Indian context. The translation of this knowledge by raising miRNA over-expressing rice plants is in progress. This will be useful to gain fundamental knowledge of plant responses to the environment leading to a range of applications for improving plant adaptation and sustaining yields. To gain an insight into the mechanism of miRNA (dis)-regulation studies are being done using viral suppressors of RNAi. This work involves understanding the role of suppressors in local and systemic silencing of miRNAs and identification of host interacting factors to gain an understanding of the suppressor mediated action. Suppressor overexpressing transgenic lines have been generated to follow the effects on miRNA expression profiles.

Selected publications

Mittal D., Sharma N., Sharma V., Sopory S.K., Sanan-Mishra N. 2016. Role of microRNAs in rice plant under salt-stress. Annals Applied Biol 168, 2-18

Saraf S., Sanan-Mishra N., Gursanscky N.R., Carroll B.J., Gupta D., Mukherjee S.K. 2015. 3’ and 5’ microRNA-end post-biogenesis modifications in plant transcriptomes: evidences from small RNA next generation sequencing data analysis. Biochem Biophys Res Commun 467, 892-899 PubMed link

Shafrin F., Das S.S., Sanan-Mishra N., Khan H. 2015. Artificial miRNA- mediated down-regulation of two monolignoid biosynthetic genes (C3H and F5H) cause reduction in lignin content in jute. Plant Mol Biol 89, 511-552 PubMed link

Tripathi A., Goswami K., Sanan-Mishra N. 2015. Role of bioinformatics in establishing miRs as modulators of abiotic stress responses: the new revolution. Front Physiol 6, 286 PubMed link

Das S.S., Karmakar P., Nandi A.K., Sanan-Mishra N. 2015. Small RNA mediated regulation of seed germination. Front Plant Sci 6, 828 PubMed link

Kumar V., Karjee S., Rehman J., Taneja J., Sundaresan G., Sanan-Mishra N., S.K. Mukherjee. 2015. Mungbean yellow mosaic Indian virus encoded AC2 protein suppresses RNA silencing by inhibiting Arabidopsis RDR6 and AGO1 activities. Virology. 486, 158–172 PubMed link

Ngoc T.T., Sanan-Mishra N. (2015) Effect of antibiotics on callus regeneration during transformation of IR 64 rice. Biotechnology Reports. 7, 143-149

Sharma N., Panchal S., Sanan-Mishra N. (2015) Protocol for artificial microRNA mediated over-expression of miR820 in indica rice. American Journal of Plant Sciences. 6, 1951

Sharma N., Tripathi A., Sanan-Mishra N. (2015) Profiling the expression domains of a rice-specific microRNA under stress. Frontiers in Plant Science. 6, 333

El Sanousi R.S., Abdelmula A.A., Mohammed I.A., Sanan-Mishra N., Hamza N.B. (2015) Evaluation of Performance and Variability of Six Sorghum Genotypes under Salinity Stress. International Journal of Research Studies in Biosciences. 3, 23-27

Das S.S., Sanan-Mishra N. (2015) A direct method for genetically transforming rice seeds with suppressors of RNAi. Plant Cell Tissue and Organ Culture: (Journal of Plant Biotechnology). 120, 277-289

Rahman J., Sanan-Mishra N., Mukherjee S.K. (2014) RNAi-hairpin mediated gene silencing and suppression of the silencing with Geminivrial MYMIV-AC2 protein. Plant Biotechnology Reports 8, 337-347

Das S.S., Sanan-Mishra N. (2014) Comparative analysis of RNAi Suppression Activity of proteins from two disparate viruses. American Journal of Plant Sciences 5, 1789-1798

Kumar, V., Anand, A., Mukherjee, S.K., Sanan-Mishra, N. 2013. Engineering Viral Suppressors of RNA Silencing: requirement and applications. In “Genetically Engineered Crops in Developing Countries.” Eds A. Kumar, G. Lobenstein and D.V.R. Reddy, Studium Press Texas, USA.

Ngoc, T.T., Ta, H.A., Nang, V.D., Sanan-Mishra, N. 2013. Isolation and molecular analysis of SRBSDV isolates infecting rice in Vietnam. Asian Journal of Agriculture and Food Sciences. 1, 5, 258-263

Anand, A., Mukherjee, S.K., Sanan-Mishra, N. 2013. Tools for pathogenicity: virus encoded RNA silencing suppressors. In “Recent Microbial pathogens and strategies for combating them: science, technology and education” Microbiology Book Series, number 4. Ed A. Mendez-Vilas, Formatex Research Center, Spain 751-763

Sanan-Mishra, N., Karjee, S., Mukherjee, S.K. 2013. RNAi technology. In “Biotechnology in Medicine and Agriculture: Principles and Practices”, Eds Kumar A., Pareek A., Gupta SM., I.K. International Publishing House, India pp 316-337

Mittal, D., Mukherjee, S.K., Vasudevan, M., Sanan-Mishra, N. 2013. Identification of tissue-preferential expression patterns of rice miRNAs. Journal of Cellular Biochem 114, 2071–2081 PubMed link

Sanan-Mishra, N., Varanasi, S.P.R.M., Mukherjee, S.K. 2013. Microregulators of Auxin action. Plant Cell Rep 32, 6, 733-740 PubMed link

ICGEB New Delhi

ICGEB Campus
Aruna Asaf Ali Marg
110 067 New Delhi
INDIA
Tel: +91-11-26741358/1007
Fax: +91-11-26742316
icgebicgeb.res.in

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