Research Groups

Molecular Virology

Research Interests and Description

Group Leader: Alessandro Marcello, PhD

Group Members

Research Interests

Virus, tick-borne encephalitis, Dengue, Zika, Chikungunya, virus-host interaction, diagnostics, point of care testing.

Description of Research

The main focus of the laboratory is the molecular and cell biology of virus-host interactions. The Group has a long-standing interest in HIV-1 transcriptional and post-transcriptional nuclear regulation and has recently become interested in hepatitis C virus (HCV) and in vector-borne viruses, such as tick-borne encephalitis virus (TBEV) and Zika virus (ZIKV). To this end, the lab has developed a platform of tools for the study of the virus-host crosstalk: from single protein-protein interactions to the 3D dynamics of host and viral components. Great effort has been devoted to the development of techniques that enable the study of virus-host interactions at high resolution and in living cells. During the past few years, the lab, in addition to photobleaching techniques, has developed a unique method to visualize viral RNAs in real time in living cells.
We are also interested in implementing viral diagnostics for developing countries by technically supporting local scientists and by developing point-of-care portable devices in collaboration with industrial partners.
Finally, chemical compounds synthesized in collaborating laboratories are tested for antiviral activity. Related to the group are the following:

Recent Publications

Çevik, R.E., Cesarec, M., Filipe, A., Licastro, D., McLauchlan, J., Marcello, A. 2017. Hepatitis C virus NS5A targets the nucleosome assembly protein NAP1L1 to control the innate cellular response, J Virol. 91 PubMed link

Tagliabue, G., Faoro, V., Rizzo, S., Sblattero, D., Saccani, A., Riccio, G., Bellini, T., Salina, M., Buscaglia, M., Marcello, A 2017. A label-free immunoassay for Flavivirus detection by the Reflective Phantom Interface technology. Biochem Biophys Res Commun. May 10. doi: 10.1016/j.bbrc.2017.05.025 PubMed link

Maistriau, M., Carletti, T., Zakaria, M.K., Braga, L., Faoro, V., Vasileiadis, V., Marcello, A. 2017. A method for the detection of virus infectivity in single cells and real time: towards an automated fluorescence neutralization test. Virus Res 237, 1-6 PubMed link

Albornoz, A., Carletti, T., Corazza, G,. Marcello, A. 2014. The stress granule component TIA-1 binds Tickborne Encephalitis Virus RNA and is Recruited to Perinuclear Sites of Viral Replication to inhibit viral translation. J Virol 88 (12):6611-22. PubMed link

Miorin, L., Romero-Brey, I., Maiuri, P., Hoppe, S., Krijnse-Locker, J., Bartenschlager, R., Marcello, A. 2013. Three dimensional architecture of tick-borne encephalitis virus replication sites and trafficking of the replicated RNA. Journal of Virology. PubMed link

Van Lint, C., Bouchat, S., Marcello, A. 2013. HIV-1 transcription and latency: an update. Retrovirology 26;10:67.PubMed link

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AREA Science Park
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34149 Trieste, ITALY
Tel: +39-040-37571
Fax: +39-040-226555
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