Research Groups

Molecular Medicine: ICGEB-EMORY Vaccine Program

Research Interests and Description

Group Leader: Anmol Chandele, PhD

Group Members

Research Interests

Human immunology of infectious diseases, vaccine research and therapeutics.

Description of Research

The ICGEB-Emory Vaccine Program, is a unique partnership between ICGEB and Emory Vaccine Center, Emory University (Atlanta, GA, USA).  The goal of this partnership is to facilitate international collaborations in vaccine research for tackling infectious diseases of public health importance in developing countries. This partnership provides an opportunity for students, fellows, scientists and clinicians in India, to collaborate with international experts in human immunology of infectious diseases and vaccine research in order to address complex biological questions that are pertinent to infectious diseases of public health importance in India.

With support from NIH, DBT and Indo-US Vaccine Action Program, our present focus is to understand human immunity to infectious diseases, with particular emphasis on Dengue in India. We will use this knowledge for identifying novel biomarkers, therapeutics and for improving vaccine design, testing and evaluation. 

Our Program has recently received a National Institutes of Health (NIH, USA) award for the study of Dengue virus infection in India.  The award is part of the NIH International Collaborations in Infectious Disease Research (ICIDR) program, a competitive grant program aimed at U.S. and collaborating foreign institutions studying infectious diseases of public health significance in resource-constrained countries.

By training young scientists in setting up state of the art immunology tools and techniques (eg high throughput human monoclonal antibody production, single cell sorting, MHC tetramer technology, T cell epitope mapping, single cell intracellular cytokine assays, and somatic hypermutation analysis) in the lab at ICGEB, the goals of the ongoing research include:

1. Using systems biology to define the molecular signatures of innate and adaptive immune responses during dengue infection.
2. To determine whether IgG Fc glycan composition is a determinant of antibody-dependent enhancement of secondary dengue virus infection and whether Fc glycan composition may have a role in the pathogenesis of severe dengue disease
3. To characterize the human B cell response during dengue infection and generate dengue virus specific monoclonal antibodies from plasmablasts.
4. To characterize CD4 and CD8 T cell responses in dengue patients
5. To generate human monoclonal antibodies that may provide novel avenues towards dengue therapeutics.  

Relevant links to Emory:

http://www.vaccines.emory.edu/

http://vaccines.emory.edu/faculty/kaja_murali_krishna.html

http://vaccines.emory.edu/faculty/affiliate/chandele_anmol.html

Recent Publications

Nayak, K., Jing, L., Russell, R.M., Davies, D.H., Hermanson, G., Molina, D.M., Liang, X., Sherman, D.R., Kwok, W.W., Yang, J., Kenneth, J., Ahamed, S.F., Chandele, A., Murali-Krishna, K., Koelle, D.M. 2015. Identification of novel Mycobacterium tuberculosis CD4 T-cell antigens via high throughput proteome screening. Tuberculosis (Edinb) 95, 275-87 PubMed link

Carpenter, C., Sidney, J., Kolla, R., Nayak, K., Tomiyama, H., Tomiyama, C., Padilla, O.A., Rozot, V., Ahamed, S.F., Ponte, C., Rolla, V., Antas, P.R., Chandele, A., Kenneth, J., Laxmi, S., Makgotlho, E., Vanini, V., Ippolito, G., Kazanova, A.S., Panteleev, A.V., Hanekom, W., Mayanja-Kizza, H., Lewinsohn, D., Saito, M., McElrath, M.J., Boom, W.H., Goletti, D., Gilman, R., Lyadova, I.V., Scriba, T.J., Kallas, E.G., Murali-Krishna, K., Sette, A., Lindestam Arlehamn, C.S. 2015. A side-by-side comparison of T cell reactivity to fifty-nine Mycobacterium tuberculosis antigens in diverse populations from five continents. Tuberculosis (Edinb). 95, 713-21. PubMed link

Wrammert, J., Murali-Krishna, K. 2014. Cellular screens to interrogate the human T and B cell repertoires and design better vaccines. Vaccine design 

Kulkarni RR, Rasheed MA, Bhaumik SK, Ranjan P, Cao W, Davis C, Marisetti K, Thomas S, Gangappa S, Sambhara S, Murali-Krishna K. 2014. Activation of the RIG-I pathway during influenza vaccination enhances the germinal center reaction, promotes T follicular helper cell induction, and provides a dose-sparing effect and protective immunity. J Virol. 88:13990-4001 PubMed Link

Wrammert J, Onlamoon N, Akondy RS, Perng GC, Polsrila K, Chandele A, Kwissa M, Pulendran B, Wilson PC, Wittawatmongkol O, Yoksan S, Angkasekwinai N, Pattanapanyasat K, Chokephaibulkit K, Ahmed R. 2012. Rapid and massive virus-specific plasmablast responses during acute dengue virus infection in humans. J Virol. 86: 2911-2918 PubMed link


PubMed links:

Murali-Krishna Kaja: http://www.ncbi.nlm.nih.gov/pubmed/?term=murali-krishna+K

Anmol Chandele: http://www.ncbi.nlm.nih.gov/pubmed/?term=Chandele+A

ICGEB New Delhi

ICGEB Campus
Aruna Asaf Ali Marg
110 067 New Delhi
INDIA
Tel: +91-11-26741358/1007
Fax: +91-11-26742316
icgebicgeb.res.in

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