Research Groups

Dinkar Sahal

Malaria Drug Discovery

Group Leader

Research Interests and Description

Dinkar Sahal

International Centre for Genetic Engineering and Biotechnology
Aruna Asaf Ali Marg
110 067 New Delhi, India

E-mail: dinkaricgeb.res.in
Office tel: +91-11-26742357
Office fax: +91-11-26742316

Education

All India Institute of Medical Sciences, New Delhi, India, PhD, 1984
MS University, Baroda, India, MSc, 1977

Career History

Since 2016, Group Leader, Malaria Drug Discovery, International Centre for Genetic Engineering and Biotechnology, ICGEB New Delhi, India
1992-2015, Staff Research Scientist, Malaria Group, ICGEB New Delhi, India
1988-1992, Assistant Professor, Department of Biophysics, University of Delhi, South Campus, New Delhi, India
1986-1988, Research Fellow, Department of Molecular Genetics, Beckman Research Institute of the City of Hope, Duarte, California
1984-1986, Research Associate, Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India
1977-1984, Senior Research Fellow, Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India

Scientific Activity

His major interest focuses the beauty of Natural Products and Synthetic molecules to ask fundamental questions in biology and to harness the power of the two to tackle the serious problems of antibiotic resistance posed by microbes and drug resistance in parasites like the malaria parasite. With major goal as discovery of safe, potent and affordable novel drugs against diseases, the major tools deployed in pursuit of the above goals are high through put screening methods, activity guided chromatographic isolation. Over the last decade, his researches have been funded majorly by Department of Biotechnology, Department of Science and Technology and the Indian Council of Medical Research. His laboratory has derived immense strength through excellent collaborations with several sister laboratories in India and abroad.

Sahal has been reviewing manuscripts written by friends, and papers or proposals received from grant giving agencies like the DBT/DST/CSIR. He has been a reviewer of papers sent by various journals like Biochimie, Analytical Biochemistry,Antimicrobial Agents and Chemotherapy, Process Biochemistry, Blood, Current Science, J PhotoBiol & Photochem, J of Biomedicine and Biotechnology,  PLOS 1, Pharmaceutical Biology, European Journal of Medicinal Chemistry, European Journal of Medicinal Plants, Journal of Complementary and Integrative Medicine, ACS Medicinal Chemistry Letters, Bioorganic & Medicinal Chemistry, Pharmacognosy and Phytochemistry, FEBS Journal, BMC Complementary and Alternative Medicine etc. He has reviewed proposals received from professors of Delhi University, National Medical Research Council and has made Site Visits as a DBT expert to various pharma companies all across India. Sahal has been a member of several task forces of the DBT including Medicinal and Aromatic Plants and Aquaculture and Marine Biotechnology and (c) Task force on MAP committee of Twinning R&D program (Drug Discovery & MAP) for NER. (d) Member of the Technical Evaluation Committee (Translational Research), Ministry of Health and Family Welfare, Government of India, Department of Health Research, (e) Expert Committee Meeting of Twinning R&D program for NER Medicinal and Aromatic Plants Biotechnology and Drug Development (MAP). He is a Member of the special committee of the School of Biotechnology of the Jawahar Lal Nehru University, New Delhi. Sahal is a member of the Indian Biophysical Society and the Indian Peptide Society. Sahal is an avid reader, has a special penchant for writing and is quite fond of speaking at scientific meetings. Dinkar Sahal is the author of Synthetic Life and Artificial Organisms (History of Science, Philosophy and Culture in Indian Civilization) and together with Dr.Vijay Kumar he has authored the chapter on Genetic Engineering for the Ullman’s encyplopedia  of Industrial Chemistry.

Teaching Activity

1988 -1992: Taught students of M.Sc. (Biochemistry) courses in buffers, chromatography, peptide and protein structure and function, spectroscopy and bioenergetics.

1993-2018: Taught Ph.D.students at ICGEB, New Delhi courses in chromatography with special reference to  purification and characterization of recombinant proteins, Introduction and fundamental questions in cell biology and Printing live cells. At ICGEB workshops I have taught subjects like NMR of bioactive natural products and purification and characterization of recombinant proteins. At Mahabaleshwar seminars I have taught Courses in Design of peptides for structure and function.

Selected publications

Srinivasan Sankarana ,  Muthukumaran Malarvel, Gopalakrishnan Sethumadhavan, Dinkar Sahal. 2017. Quantitation of Malarial parasitemia in Giemsa stained thin blood smears using Six Sigma threshold as preprocessor.  Optik 145 225–239.

Varatharajan Sabareesh and Dinkar Sahal. 2015. Explorations into conformationally distinct isomeric peptides of opposite directionalities by high resolution electrospray collision induced dissociation tandem mass spectrometry. International Journal of Mass spectrometry. 391, 31-40.

Niharika Singh, Naveen Kumar Kaushik, Dinesh Mohanakrishnan, Santosh Kumar Tiwari, Dinkar Sahal. 2015. Antiplasmodial Activity of Medicinal Plants from Chhotanagpur Plateau, Jharkhand, India. Journal of Ethnopharmacology 165:152-62.

Abhijeet Mishra, Naveen Kumar Kaushik, Meryam Sardara, Dinkar Sahal. 2013. Evaluation of antiplasmodial activity of green synthesized silver Nanoparticles. Colloids and Surfaces B: Biointerfaces 111 713– 718.

Naveen K   Kaushik, Jyotsna Sharma and Dinkar Sahal. 2012. Anti-plasmodial action of de novo-designed, cationic, lysine-branched, amphipathic, helical peptides. Malaria Journal 11:256.

Vijay Kumar and Dinkar Sahal. 2014. Genetic Engineering, Ullmann’s Encyclopedia of Industrial Chemistry

Sharma, N, Mohanakrishnan D, Shard A, Sharma A, Malik S, Sinha, A, Sahal D. Stilbene-Chalcone Hybrids: Design, Synthesis and Evaluation as a New Class of Antimalarial Scaffolds that Trigger Cell Death through Stage Specific Apoptosis. 2012. J Med Chem., 55, 297-311.

Sayani Dasgupta, Sharmishtha Samantaray, Dinkar Sahaland Rajendra P Roy. 2011. Isopeptide Ligation Catalyzed by Quintessential Sortase A: Mechanistic Cues from Cyclic and Branched Oligomers of Indolicidin. THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 286, NO. 27, 23996–24006.

Ipsita Pal-Bhaumick, Ramendra Pati Pandey, Gotam K. Jarori, Santosh Kar, Dinkar Sahal. 2007. Structural and functional studies on Ribonuclease S, Retro S and Retro Inverso S peptides.  Biochem Biophys Res Commun. 364(3):608-13.

Sahal, D. 2009. “Synthetic Life and Artificial Organisms”, chapter in the volume “Life and Organicism”: Project on History of Indian Science, Philosophy and Culture (PHISPC) Rangaswamy, N.S. (ed)

Pooja C.Dewan, Aparna Anantharaman, Virander S Chauhan and Dinkar Sahal 2009. Antimicrobial Action of Prototypic Amphipathic Cationic Decapeptides and Their Branched Dimers. Biochemistry, 48, 5642-5657.

Anantharaman, Meryam Sardar and Dinkar Sahal. 2010. Synergy with rifampicin and kanamycin enhances potency, kill kinetics and selectivity of de novo designed antimicrobial peptides. Antimicrobial Agents and Chemotherapy, 54,1693-1699.

Aparna Anantharaman and Dinkar Sahal. 2010.Reverse Engineering Truncations of an antimicrobial peptide dimer to identify the origins of potency and broad spectrum of action. J Med Chem, 53 (16), 6079–6088.

Sahal, D, Kannan R, Chauhan V.S.2003. Applying malaria parasite's heme detoxification system for screening potential antimalarial drugs. Anal Biochem. 312(2):258-60.

Rudresh; Ramagopal, U.A.; Inai, Y.; Goel, S.; Sahal, D.; Chauhan, V.S.2004.  De novo design and characterization of a helical hairpin eicosapeptide: Emergence of an anion receptor in the linker region. Structure 12, 389-396.

Udupi A. Ramagopal,Suryanarayanrao Ramakumar, Dinkar Sahal and Virander  S.Chauhan. 2001. De novo design and characterization of an apolar helical hairpin peptide at atomic resolution: Compaction mediated by weak interactions. Proc. Natl. Acad. Sci (USA) 98 (3), 870 - 874.

Hasan Korkaya, Shahid Jameel, Dinesh Gupta, Shweta Tyagi, Ravinder Kumar, Mohammad Zafrullah, Manjari Mazumdar, Sunil Kumar Lal, Li Xiaofang, Deepak Sehgal, Suman Ranjan Das, and Dinkar Sahal 2001. The ORF3 protein of hepatitis E virus binds to Src homology 3 domains and activates   MAPK. J Biol Chem. Nov 9; 276(45):42389-400.

Sahal D, Kannan R, Sinha A, Babbarwal V, Gnana Prakash B, Singh G, Chauhan VS. 2002. Specific and instantaneous one-step   chemodetection of histidine-rich proteins by Pauly's stain. Anal Biochem. 308 (2):405-8.

Kannan R, Sahal, D, Chauhan V.S. 2002. Heme-artemisinin adducts are crucial mediators of the ability of artemisinin to inhibit heme polymerization. Chem Biol. Mar; 9 (3): 321-32.

Dinkar Sahal and Yoko Fujita-Yamaguchi. 1989. Solid-phase tyrosine specific protein kinase assay in multiwall substrate-immobilized polyacrylamide gel. Anal. Biochem 182, 37-43.

Dinkar Sahal, Shu-Lian Li and Yoko Fujita-Yamaguchi. 1991. Solid-phase protein kinase assay. Methods in Enzymology 200, 90-98.

Sahal, D; and Balaram, P. 1986. Peptide models of electrostatic interactions in proteins:nmr studies on two beta turn tetra peptides containing Asp---His and Asp---Lys salt bridges. Biochemistry 25, 6004-6013.

Sahal, D; and Fujita-Yamaguchi,Y. 1987. Protein kinase assay by paper trichloroacetic acid method: high performance using phosphocellulose paper and washing an ensemble of samples on flat sheets. Anal. Biochem 167, 23-30.

Talwar,G.P; Gupta S.K ; Singh,V; Sahal,D; Iyer,K.S.N; and Singh,O. 1985. Bioeffective monoclonal antibody against the decapeptide gonadotropin release hormone: reacting determinants and action on ovulation and estrus suppression. Proc.Natl.Acad.Sci.U.S.A 82,1228-1231.

ICGEB New Delhi

ICGEB Campus
Aruna Asaf Ali Marg
110 067 New Delhi
INDIA
Tel: +91-11-26741358/1007
Fax: +91-11-26742316
icgebicgeb.res.in

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