| Patents |
| Species/Family-specific Procedures |
CHLOROPHYLLOUS TOTIPOTENT CELL CULTURES, S P Jayakumar, N Hopkins, J F Petolino & D Pareddy (2005). Dow Agrosciences LLC, USA. Patent # WO2005070066.
The subject invention provides totipotent, chlorophyllous, cell cultures of maize. In addition, the methods of producing such cultures are applicable to other related species, including cereals such as rice, oats, barley, and wheat. The subject cultures are valuable for herbicide studies, studies for enhancing photosynthesis, and genetic manipulation, such as plastid transformation. The methods of the subject invention are capable of providing high percentages of totipotent cells. These cells are capable of sustained cell division and are competent for regeneration over long periods; they provide high-quality target tissue for nuclear and organelle transformation. The invention also describes methods for the introduction of heterologous DNA into the chloroplast genome. The present invention also provides methods, vectors, and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. |
CHLOROPLAST TRANSFORMATION OF DUCKWEED, K M Cox & C G Peele (2005). Biolex Inc., USA. Patent # WO2005005643.
The present invention provides methods and compositions for the transformation of duckweed plastids. The compositions and methods of the invention are useful in increasing the recombinant protein production capacity of the duckweed expression system. The compositions of the invention include transformed duckweed plastids and transplastomic duckweed cells and plants, as well as nucleic acid constructs useful for transforming duckweed plastids. The invention also provides methods for introducing one or more heterologous nucleotide sequences into a duckweed plastome. |
PLASTID TRANSFORMATION OF TOBACCO SUSPENSION CELLS, C Langbecker, J M Staub & G Ye (2004). Monsanto Technology LLC, USA. Patent # US2004199937.
A method is provided for transforming undeveloped plastids in tobacco suspension culture to produce a transplastomic plant. |
METHOD OF PLASTID TRANSFORMATION IN ASTERACEAE, VECTOR FOR USE THEREIN AND PLANTS THUS OBTAINED, J M Nugent, C L C Lelivelt & M S McCabe (2004). Rijk Zwaan Zaadteelt En Zaadha BV, the Netherlands. Patent # CA2494986.
The present invention relates to a method for the transformation of plastid genomes of plant species, in particular Asteraceae plant species, comprising the steps of providing a transformation vector carrying a DNA sequence of interest; subjecting a plant material, which comprises plastids, to a transformation treatment in order to allow the plastids to receive the transformation vector; placing the thus treated plant material for a period of time into contact with a culture medium without selection agent; subsequently placing the plant material into contact with a culture medium comprising a selection agent; and refreshing the culture medium comprising a selection agent to allow plant material comprising plastids that have acquired the DNA of interest to grow into transformant. |
FERTILE, TRANSPLASTOMIC LEGUMINOUS PLANTS, USEFUL E.G. FOR PREPARING PHARMACEUTICALS, ALSO FOR IMPROVING PLANT RESISTANCE OR AGRONOMIC PROPERTIES, AND VECTORS FOR HOMOLOGOUS RECOMBINATION WITH PLASTID SEQUENCES, G Tissot, N Dufourmantel, F Garcon, J M Ferrulo & B Pelissier (2004). Bayer Cropscience SA, France. Patent # FR2848064.
Fertile, transplastomic leguminous plants (A), are new. Independent claims are also included for: (1) vector (V) for transformation of leguminous plastids comprising at least two sequences (I) that are homologous with a region of the plastome being transformed and flanking at least one expression cassette (EC); and (2) producing (A). |
PLASTID TRANSFORMATION OF SOLANACEOUS PLANTS, N S Nehra, D J Schaaf, V Sidorov, D J Stalker & G Ye (2003). Calgene LLC, USA. Patent # US2003177521.
A method is provided for transforming Solanaceous plants to express DNA sequences interest from the plant cell plastid. The improved method allows the transformation of Solanaceous plant tissue which is not obtained from tobacco with DNA constructs. Such DNA constructs comprise, in the 5' to 3' direction of transcription, a promoter region functional in a plant plastid and a DNA sequence of interest. The method can be utilised in the transformation of Solanaceous plants, such as potato and petunia. The invention further provides constructs and methods for the expression of green fluorescent protein from the plant cell plastid. |
PLASTID TRANSFORMATION IN LESQUERELLA FENDLERI, AN OILSEED BRASSICA (), P Maliga, M Skarjinskaia & Z S Maliga (2003). Rutgers University, USA. Patent # US2003200568.
This invention relates to methods and compositions for obtaining Arabidopsis and Brassica plants. Specifically, the method provides culturing protocols and compositions that facilitate the regeneration of transformed plants following delivery of beneficial DNA molecules. |
POPLAR CHLOROPLAST SITE-SPECIFIC TRANSFORMATION METHOD AND DNA SEQUENCE FOR SAID METHOD, Y Zhou, L Zhang & Z Chen (2002). Institute of Genetics, China. Patent # CN1380417.
The present invention provides a method for site-directed transforming exogeneous gene into poplar chloroplast, and said method includes the folloiwng steps: using DNa recombinant fragment constituted by using sequence indicated by SEQ ID No:1 or its fragment which is not less than 1Kb, sequence indicated by SEQ ID No:2 or its fragment which is not less than 1 kb and an exogenous gene; positioning the exogenous gene between SEQ ID No:1 or its fragment and SEQ ID No:2 or its fragment; inserting the obtained DNA recombinant fragment into a cloning vector to obtain the poplar chloroplast genome site-directed recombinant transforming vector, using obtained vector to transformer poplar cell; and breeding the transformed poplar cell to obtain plant. It also provides the DNA sequence used for said method. |
PLASTID TRANSFORMATION OF LYCOPERSICON PLANTS, R Bock & H Carrer (2002). Greenovation Biotech GMBH, Germany. Patent # WO02078429.
The present invention provides methods for obtaining stably transformed transplastomic plant cell material belonging to the genus Lycopersicon (tomato) and having the potential to regenerate into mature fertile plants. The method generally comprises (a) transforming plastids of said plant cell material with a DNA molecule carrying an expression cassette encoding at least one protein of interest, and a target sequence enabling homologous recombination; (b) selecting plant cell material until substantially all plastids have been transformed with said DNA molecule, thereby obtaining said stably transformed transplastomic plant cell material; and, optionally (c) regenerating said transplastomic plant cell material into mature fertile plants. Furthermore, the present invention provides stably transformed transplastomic Lycopersicon plant cells, seeds, tissues and organs having the potential to regenerate into mature fertile plants, as well as mature fertile plants, obtainable or obtained by carrying out the methods according to the invention. |
METHODS FOR GENOTYPE-INDEPENDENT NUCLEAR AND PLASTID TRANSFORMATION COUPLED WITH CLONAL REGENERATION UTILIZING MATURE ZYGOTIC EMBRYOS IN RICE (ORYZA SATIVA) SEEDS, N A Reichert, V Rudraswamy & L M-J Chen (2000). University of Mississippi, USA. Patent # US6153813.
A method for transforming rice plants to express heterologous DNA which involves the biolistic bombardment of mature rice seeds (embryo bearing) and results in expression of the heterologous DNA coated on the biolistic particle in the post-bombardment embryo. The heterologous DNA may be introduced as a plastid, as opposed to bare DNA. The transformed rice embryo is regenerated into a rice plantlet which can be transferred from greenhouse to field. |
PLASTID TRANSFORMATION OF BRASSICA, S Chaudhuri & J V Oakes (2000). Calgene LLC, USA. Patent # WO0039313.
A method is provided for transforming Brassica plants to express DNA sequences of interest from the plant cell plastid. The method allows the transformation of Brassica plant tissue with heterologous DNA constructs. Such DNA constructs comprise, in the 5' to 3' direction of transcription, a promoter region functional in a plant plastid and a DNA sequence of interest. The invention further provides for Brassica cells in which the plastids contain heterologous DNA constructs. |
PLASTID TRANSFORMATION IN ARABIDOPSIS THALIANA, P Maliga, S R Sikdar & S V Reddy, (1997). Rutgers University, USA. Patent # WO9732977.
This invention provides methods and compositions for obtaining transplastomic Arabidopsis and Brassica plants. Specifically, the method provides culturing protocols and compositions that facilitate the regeneration of transformed plants following delivery of exogenous, beneficial DNA molecules. |
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