| Patents |
| Herbicide Tolerance |
EXPRESSION OF HYDROXYPHENYLPYRUVATE DIOXYGENASE IN PLASTIDS OF PLANTS FOR HERBICIDE TOLERANCE, G Tissot, J-P Wisniewski & J-M Ferullo (2004). Biogemma, France. Patent # WO2004055191.
The invention relates to transplastomic plants with enhanced tolerance for herbicides based on inhibitors of hydroxyphenylpyruvate dioxygenase (HPPD), a method for generating such plants, and the vectors used. |
ISOLATED POLYNUCLEOTIDE ABLE TO CONFER TO PLANT RESISTANCE OR TOLERANCE TO GLYPHOSATE HERBICIDE, VECTOR, METHOD FOR PREPARING PLANTS WITH TOLERANCE OR RESISTANCE TO GLYPHOSATE HERBICIDE, METHOD FOR REGENERATION OF TRANSFORMED PLANT AND METHOD FOR SELECTIVE CONTROL OF WEEDS, T R Hawkes, S A J Warner, J Christopher, S Bachoo & A P Pickerill (2004). Syngenta Ltd., Switzerland. Patent # RU2235778.
The invention relates to technology of recombinant DNAs that are required for preparing transgenic plants with resistance or eliciting significant tolerance to herbicides. Method involves isolation of polynucleotide encoding chloroplast signal peptide and glyphosate-resistant rice 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) located by course of transcription after site encoding chloroplast signal peptide. Expression of indicated site is under control of vegetable functional promoter that is not heterologous with respect to indicated site and chloroplast signal promoter is not heterologous with respect to indicated synthase. Indicated components are in sequence by direction 5' 3' of transcription with possibility of variants in disposition of enhancers. Taken together, the sequence encoding rice EPSPS is modified by such manner that by the first position in conservative site GNAGTAMRPLTAAV in enzyme of wild type residue Thr is replaced for residue Ile and by the second position residue Pro is replaced for Ser and thus the modified sequence represents GNAGIAMRSLTAAV. Indicated polynucleotide is inserted in vector construction that is used for transformation of vegetable material. Prepared transformed material is subjected for preparing whole plants with resistance or tolerance to glyfosate herbicide. Regeneration involves preparing plant tissue that will be transformed, placing tissue transformed with above indicated polynucleotide or vector in nutrient medium containing compound providing possibilities for identification or selection of transformed tissues taken for regeneration, transfer of formed, at least one, sprout in the second medium promoting to formation of roots and culturing indicated sprout up to stage of adult plant that is able to multiplication. Field with agriculture transgenic plants and contaminated with weeds is treated with glyfosate taken in effective dose to control weeds and without significant effect on agricultural plants. |
HERBICIDE TOLERANCE ACHIEVED THROUGH PLASTID TRANSFORMATION, P B Heifetz, S L Volrath, M A Johnson & E R Ward (2002). Syngenta Biotechnology Inc., USA. Patent # US2002073443.
Disclosed are novel DNA sequences isolated from soybean, wheat, cotton, sugar beet, rape, rice, sorghum, and sugar cane encode enzymes having protoporphyrinogen oxidase (protox) activity. In addition, modified, herbicide-tolerant forms of protox enzymes are disclosed. Plants expressing herbicide-tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an inhibitor-resistant from of a plant protox enzyme. Further disclosed is a method of achieving herbicide tolerance through plastid transformation, which involves transforming plastids with a chimeric gene that comprises a plastid-active promoter operatively linked to a DNA molecule that encodes a plastid-targeted enzyme whose native plastid transit peptide is either mutated so as to be nonfunctional or is absent altogether. The encoded enzyme may be for example a herbicide-tolerant protox enzyme, whereby the resulting transplastomic plants are resistant to protox inhibitors.
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METHOD FOR PRODUCING TRANSGENIC PLANTS WITH MODIFIED 5-AMINOLEVULINIC ACID BIOSYNTHESIS, METHOD FOR IDENTIFYING 5-AMINOLEVULINIC ACID SYNTHESIS EFFECTORS, F Schmidt & G Donn (2002). Aventis Cropscience GMBH, Germany. Patent # US6603062.
Provided is a method for producing transgenic monocotyledonous plants, plant cells, plant parts, seeds, and reproduction material with modified 5-aminolevulinic acid biosynthesis. This is achieved by stably integrating one or several nucleic acid molecules coding for a protein with a 5-aminolevulinic acid synthase function (ALAS) isolated from the alpha group of purple bacteria, an active fragment thereof or an antisense or complementary sequence thereof, into the plant genome in stable form. This method can also be used to control undesired vegetation. Also provided is a method for producing transgenic plants or plant cells whose glutamate-1-semialdehyde transferase (GSAAT) expression is suppressed or inhibited by stable integration of at least one nucleic acid molecule encoding an ALAS isolated from the alpha group of purple bacteria into the plant plastome by plastid transformation. |
HERBICIDE RESISTANT PLANTS, T R Hawkes, S A J Warner, J Christopher, S Bachoo & A P Pickerill (2000). Zeneca Ltd., UK. Patent # WO0066748.
The present invention provides, inter alia, an isolated polynucleotide comprising a region encoding a chloroplast transit peptide and a glyphosate resistant 5-enolpyruvylshikimate phosphate synthase (EPSPS) 3' of the peptide, the said region being under expression control of a plant operable promoter, with the provisos that the said promoter is not heterologous with respect to the said region, and the chloroplast transit peptide is not heterologous with respect to the said synthase.
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IMPROVED PLASTID TRANSFORMATION OF HIGHER PLANTS AND PRODUCTION OF TRANSGENIC PLANTS WITH HERBICIDE RESISTANCE, A D Blowers, J Sanford & A M Bailey (1999). Sanford Scientific Inc., USA & Invest Y De Estudios Avanzados, Mexico. Patent # WO9905265.
The present invention provides a method of producing a herbicide-resistant plant, which method entails delivering one or more herbicide resistance-conferring selectable marker genes into and expressing the same within the plastid of the plant, both in photosynthetic as well as non-photosynthetic cells. Nucleic acids for transformation and multicellular plants whose plastids have been transformed are also provided. |
CHIMAERIC GENE FOR THE TRANSFORMATION OF PLANTS, B Leroux, A Sailland & M Lebrun (1996). Rhone Poulenc Agrochimie, France. Patent # US5510471.
Chimaeric gene for conferring to plants an increased tolerance to a herbicide having as its target EPSPS. It comprises, in the direction of transcription, a promoter region, a transit peptide region, a coding sequence for glyphosate tolerance and a polyadenylation signal region, wherein the transit peptide region comprises, in the direction of translation, at least one transit peptide of a plant gene encoding a plastid-localised enzyme, a partial sequence of the N-terminal mature part of a plant gene encoding a plastid-localised enzyme and then a second transit peptide of a plant gene encoding a plastid-localised enzyme. Production of glyphosate-tolerant plants.
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GLYPHOSATE-RESISTANT PLANTS, D M Shah, S G Rogers, R B Horsch & R T Fraley (1987). Monsanto Co. Ltd., USA. Patent # EP0218571.
This invention involves a cloning or expression vector comprising a gene which encodes 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide which, when expressed in a plant cell contains a chloroplast transit peptide which allows the polypeptide, or an enzymatically active portion thereof, to be transported from the cytoplasm of the plant cell into a chloroplast in the plant cell, and confers a substantial degree of glyphosate resistance upon the plant cell and plants regenerated therefrom. The EPSPS coding sequence may be ligated to a strong promoter, such as the 35S promoter from cauliflower mosaic virus, to create a chimeric gene. Such genes can be inserted into plant transformation vectors, and subsequently introduced into plant cells. Plant cells transformed using such genes and plants regenerated therefrom have been shown to exhibit a substantial degree of glyphosate resistance.
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