| Patents |
| Genetic Elements |
NOVEL PLANT PLASTID PROMOTER SEQUENCE, P B Hiefetz (1999), Novartis AG, Switzerland & Novartis Erfind Verwalt GMBH, Austria. Patent #WO9946394.
A novel promoter isolated from the 5' flanking region upstream of the coding sequence of the Arabidopsis plastid clpP gene is described. Also described are a novel method for utilizing protein-coding regions of plastid genes to isolate intervening regulatory sequences and a novel method for improving plastid transformation efficiency using exogenous plastid promoters that differ in nucleotide sequence from native plastid promoters. |
TRANSIT PEPTIDE DNA SEQUENCE, M Lebrun, B Leroux & A Sailland (1999), Rhone Poulenc Agrochimie, France. Patent #EP0924299
A new ADN transit peptide sequence comprises at least one coding sequence for the transit peptide from a plant gene encoding an enzyme located in plastids, the mature part of the N-terminal sequence of the plant gene coding for an enzyme located in plastids, and/or a sequence coding for a second transit peptide from a plant gene coding for an enzyme located in plastids. Independent claims are also included for the following: (1) a chimeric gene useful in plants to confer herbicidal resistance having an EPSPS target comprising a promoter region, a transit peptide region (as above), a sequence coding for resistance to glyphosate and a polyadenylated signal region not 3' translated; (2) a process for the construction of the above chimeric gene comprising isolating at least 2 regions of the transit peptide and at least part a sequence of the mature plant plastid genes to achieve at least one sequence coding for glyphosate resistance and a polyadenylated signal region therefore altogether providing plant resistance; (3) a vector for the transformation of the plant comprising the above chimeric gene; (4) a transformed plant cell containing the above chimeric gene; (5) a transformed plant with an improved resistance to herbicides having an EPSPS target obtained using the above cell; (6) a transformed plant with an improved resistance to herbicides having an EPSPS target stems from crossing the above plants; (7) a process for obtaining hybrid cell lines having glyphosate resistance using the above plants as parent plants; and (8) a process for treating the above plants comprising applying a herbicide having an EPSPS target |
CONTROLLED EXPRESSION OF TRANSGENIC CONSTRUCTS IN PLANT PLASTIDS, K E McBride & D M Stalker David M (1995), Calgene Inc., USA. Patent #WO9516783
Novel compositions and methods useful for genetic engineering of plant cells to provide a method of controlling the timing or tissue pattern of expression of foreign DNA sequences inserted into the plant plastid genome are provided in the instant invention. Constructs include those for nuclear transformation which provide for expression of a viral single subunit RNA polymerase in plant cell tissues, and targeting of the expressed polymerase protein into the plant cell plastids. In addition, plastid expression constructs comprising a viral gene promoter region which is specific to the RNA polymerase expressed from the nuclear expression constructs described above, and a DNA sequence of interest to be expressed in the transformed plastid cells are provided. Plant cells and plants comprising the nuclear and/or plastid constructs described herein are of interest. Of particular interest is a method of controlling expression of the inserted plastid gene constructs in a tissue and/or developmental specific manner in plants comprising both the nuclear polymerase construct and the plastid expression constructs.
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CHIMERIC GENES FOR TRANSFORMING PLANT CELLS USING VIRAL PROMOTERS, R T Fraley, R B Horsch & S G Rogers (1994), Monsanto Co., USA. Patent #US5352605
In one aspect the present invention relates to the use of viral promoters in the expression of chimeric genes in plant cells. In another aspect this invention relates to chimeric genes which are capable of being expressed in plant cells, which utilise promoter regions derived from viruses which are capable of infecting plant cells. One such virus comprises the cauliflower mosaic virus (CaMV). Two different promoter regions have been derived from the CaMV genome and ligated to heterologous coding sequences to form chimeric genes. These chimeric genes have been shown to be expressed in plant cells. This invention also relates to plant cells, plant tissue, and differentiated plants which contain and express the chimeric genes of this invention.
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DNA CONSTRUCT FOR ENHANCING THE EFFICIENCY OF TRANSCRIPTION, J C McPherson & R Kay (1992), University of British Columbia, Canada. Patent #EP0189707
Novel transcription initiation regions that provide for enhanced transcription of a DNA sequence, particularly a plant sequence, are provided.
Associated patents: Patent # US5196525, 1993, University of British Columbia, Cananda and Patent # US5322938, 1994, Monsanto Co., USA. |
RECOMBINANT DNA WHICH CAN BE INTRODUCED INTO PLANT CELLS, L Herrera-Estralla, G van de Broeck, M van Montagu, P Schreier, J Schell, H J Bohnert, A R Cashmore, M P Timko & A P Kausch (1986), Bayer AG, Germany & Plant Genetic Systems NV, Belgium. Patent #EP0189707
The invention relates to chimaeric genes which encode a fusion protein consisting of a transit peptide of a chloroplast protein-precursor, linked directly or indirectly to another protein. It also relates to recombinant vectors containing said chimaeric genes suitable for the transformation of plant cells, whereby said another protein can be translocated and processed in the chloroplast. |
DNA AND VECTOR FOR TRANSFORMATION OF PLANT CELL, S-B. Lee, B-Y Lee, M-Y Eun, T-Y Jung, Y-H Park & I-K Lee (1997), Rural Development Administration, S. Korea. Patent #kr9700686
The chloroplast autoreplicating sequence (ARS) was isolated from a chloroplast of rape. A vector containing the ARS is prepared by recombining a selection marker and a promotor sequence with the said ARS. pECTRHG vector used for transforming plants or bacteria was prepared by a recombination of a replicative origin of E. coli plasmid, pUC19 having ampicillin resistant gene as the selection factor of the plant cell, and PrbcLp and PatpB as a promotor for expression in a plant microorgan.
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| Genetic Use Restriction Technologies (GURTs) |
CONTROL OF PLANT GENE EXPRESSION, M J OLIVER, J E Quisenberry, N L G Trolinder & D L Keim (1996), Delta & Pine Land Co., USA. Patents #CA2196410 & EP0775212
A method for making a genetically modified plant comprising regenerating a whole plant from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked on either side by specific excision sequences, a second gene that encodes a recombinase specific for the specific excision sequences linked to a repressible promoter, and a third gene that encodes the repressor specific for the repressible promoter. Also a method for making a genetically modified hybrid plant by hybridising a first plant regenerated from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked on either side by specific excision sequences to a second plant regenerated from a second plant cell that has been transfected with DNA sequences comprising a second gene that encodes a recombinase specific for the specific excision sequences linked to a promoter that is active during seed germination, and growing a hybrid plant from the hybrid seed. Plant cells, plant tissues, plant seed and whole plants containing the above DNA sequences are also claimed.
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